Effects of nicotine administration in rats on MMP2 and VEGF levels in periodontal membrane.

Abstract:

BACKGROUND:Nicotine is associated with increased incidence of periodontal disease and poor response to therapy. This article aimed at identifying the expression of matrix metalloproteinases 2 (MMPs2) and vascular endothelial growth factor (VEGF) proteins on extracellular matrix, fibrous distribution and angiogenetic development in periodontitis caused by nicotine effects on periodontal membrane. MATERIALS AND METHODS:In this experimental study, rats were divided into nicotine and control groups. While the rats in the nicotine group (n = 6) were administered 2 mg/kg nicotine sulphate for 28 days, the animals in the control group (n = 6) were only administered 1.5 mL physiologic saline solution subcutaneously for 28 days. RESULTS:Histological sections were prepared and immunohistochemically stained for MMP2 and VEGF. The sections stained with Trichrome-Masson were observed under light microscope. VEGF and MMP2 immunoreactivity of periodontal gingiva and dentin was assessed by immunohistochemical staining. CONCLUSIONS:Nicotine reduces MMP production, disrupts collagen synthesis and causes periodontitis. We observed that nicotine increases periodontitis by disrupting periodontal membrane and prevents tooth to anchor in dental alveoli by disrupting epithelial structure.

journal_name

Folia Morphol (Warsz)

journal_title

Folia morphologica

authors

Deveci B,Ayna B,Tacir IH,Deveci E,Tuncer MC,Pala A

doi

10.5603/FM.a2018.0004

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

471-477

issue

3

eissn

0015-5659

issn

1644-3284

pii

VM/OJS/J/56553

journal_volume

77

pub_type

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