Abstract:
:N6-methyladenosine (m6A) represents one of the most common RNA modifications in eukaryotes. Specific m6A writer, eraser, and reader proteins have been identified. As an m6A eraser, ALKBH5 specifically removes m6A from target mRNAs and inactivation of Alkbh5 leads to male infertility in mice. However, the underlying molecular mechanism remains unknown. Here, we report that ALKBH5-mediated m6A erasure in the nuclei of spermatocytes and round spermatids is essential for correct splicing and the production of longer 3'-UTR mRNAs, and failure to do so leads to aberrant splicing and production of shorter transcripts with elevated levels of m6A that are rapidly degraded. Our study identified reversible m6A modification as a critical mechanism of posttranscriptional control of mRNA fate in late meiotic and haploid spermatogenic cells.
journal_name
Proc Natl Acad Sci U S Aauthors
Tang C,Klukovich R,Peng H,Wang Z,Yu T,Zhang Y,Zheng H,Klungland A,Yan Wdoi
10.1073/pnas.1717794115subject
Has Abstractpub_date
2018-01-09 00:00:00pages
E325-E333issue
2eissn
0027-8424issn
1091-6490pii
1717794115journal_volume
115pub_type
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