Abstract：
:Ubb+1, a ubiquitin (Ub) mutant protein originating from misreading of the Ub B gene, is found accumulated in brain tissues of Alzheimer's disease patients. The mutant attracts strong interest due to its possible participation in the molecular events leading to neurodegeneration. Ubb+1 is composed of the globular domain of Ub, linked to a 19-residue C-terminal peptide. Based on NMR relaxation and solvent accessibility measurements we obtained new insight into the molecular properties of Ubb+1. We further determined the thermal stability of Ubb+1 in the monomeric form, and in Lys48- and Lys63-linked dimers. Finally, we explored the influence of the C-terminal fragment on the interactions of Ubb+1 with an isolated UBA2 domain and with membrane mimics. Our data indicate that the C-terminal fragment of Ubb+1 is overall highly flexible, except for a short stretch which appears less solvent-exposed. While influencing the hydrodynamic properties of the globular domain, the fragment does not establish long-lived interactions with the globular domain. It results that the structure and stability of Ub are minimally perturbed by the peptide extension. However, binding to UBA2 and to membrane mimics are both affected, exemplifying possible changes in biomolecular recognition experienced by the disease-associated Ubb+1 compared to the wild-type protein.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Munari F,Bortot A,Assfalg M,D'Onofrio Mdoi
10.1016/j.ijbiomac.2017.11.121subject
Has Abstractpub_date
2018-03-01 00:00:00pages
24-31eissn
0141-8130issn
1879-0003pii
S0141-8130(17)33065-9journal_volume
108pub_type
杂志文章abstract：:The purpose of this study was to investigate the immune and antioxidant activities of Glycyrrhiza glabra polysaccharides (GGP) in rats fed high-fat diet. The experiment was performed on four groups of growing Kunming mice. The results of the experiment showed a statistically significant decrease in serum antioxidant e...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2009.04.001
更新日期：2009-07-01 00:00:00
abstract：:Several models have been proposed for the quaternary structure of alpha-crystallin. Some suggest the subunits are arranged in concentric shells. Others propose that the subunits are in a micelle-like arrangement. However, none is able to satisfactorily account for all observations on the protein and the quaternary str...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(98)00023-3
更新日期：1998-05-01 00:00:00
abstract：:The purpose of this study was to develop a new therapeutic approach for atorvastatin (ATV) adopting nanostructured polymeric micelles for its controlled delivery to the cancer cells. Amphiphilic block copolymers of stearyl chitosan (SC) and sulfated stearyl chitosan (S-SC) that could self assemble to form polymeric mi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.05.026
更新日期：2012-11-01 00:00:00
abstract：:Lavender essential oil (OEL) was added to starch, furcellaran and gelatin (S/F/G) films in concentrations of 2%, 4% and 6%. The films were examined in terms of physical properties (thickness, density, water solubility, water absorption and degree of swelling) and mechanical properties (tensile strength, elongation at ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.04.014
更新日期：2018-07-15 00:00:00
abstract：:Lectins are a group of widely distributed and structurally heterogeneous proteins of nonimmune origin. These proteins have the ability to interact with glycans present on cell surfaces and elicit diverse biological activities. Machaerium acutifolium lectin (MaL) is an N-acetyl-D-glucosamine-binding lectin that exhibit...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.06.236
更新日期：2020-11-15 00:00:00
abstract：:The mitochondrial genome (mitogenome) can provide information for phylogenetic analyses and evolutionary biology. We sequenced, annotated, and characterized the mitogenome of Euproctis similis. The complete mitogenome is 15,437bp in length, containing 13 protein-coding genes (PCGs), 22 transfer RNA genes, two ribosoma...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.07.033
更新日期：2017-12-01 00:00:00
abstract：:In this study, the microbiological, physicochemical, and flavor changes of turbot (Scophthalmus maximus) coated with a composite active coating of locust bean gum (LBG) and sodium alginate (SA) supplemented with daphnetin emulsions (0.16, 0.32, 0.64 mg·mL-1) were determined during 18 days of refrigerated storage (4 ± ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.12.089
更新日期：2021-02-15 00:00:00
abstract：:Aqueous solutions of reline and glyceline, the most common deep eutectic solvents, were used as a medium for Catalase reaction. By some spectroscopic methods such as UV-vis, fluorescence and circular dichroism (CD) function and structure of Catalase were investigated in aqueous solutions of reline and glyceline. These...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.11.043
更新日期：2017-02-01 00:00:00
abstract：:Naked barley grains were germinated at 25 °C for 12, 24, and 36 h, followed by hot air and infrared drying. Changes in structural, physicochemical, and functional properties of naked barley flour and starch after germination and drying were evaluated. Germinated and dried flour showed loose structure in starch granule...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.10.114
更新日期：2020-12-15 00:00:00
abstract：:In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode. The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC)...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.06.019
更新日期：2015-09-01 00:00:00
abstract：:The application of iron oxide nanoparticles [IONPs] in biomedical research is progressively increasing, leading to the rapid development of biocompatible and surface modified IONPs. However, there is still a need of information pertaining to its cellular and acute toxicity profile. This work reports the synthesis of β...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.09.067
更新日期：2018-04-15 00:00:00
abstract：:Chitin is a ubiquitous renewable biopolymer that is significantly distributed in the natural world. Biopolymeric nanoparticles (Nps) have been developed for various biomedical applications by researchers. Here, chitin derivative, dibutyrylchitin Nps (DBC) was synthesized as a nanocarrier for drug delivery using butyri...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.11.031
更新日期：2016-01-01 00:00:00
abstract：:Regenerated chitin (RCh)/poly (lactic acid) (PLA) blends with enhanced properties were successfully prepared via the Pickering emulsion approach. An oil-in-water Pickering emulsion of PLA/dichloromethane (DCM) stabilized with RCh via an ultrasonic homogenizer was formed. A uniformly dispersed blends were obtained upon...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.08.117
更新日期：2019-11-01 00:00:00
abstract：:A novel biopolymer-based superporous hydrogel (SPH) was synthesized through chemical crosslinking by graft copolymerization of 2-hydroxyethyl methacrylate (HEMA) and acrylic acid (AA) on to xanthan gum (XG) via redox initiator system of ammonium persulfate (APS) and N, N, N', N'-tetramethylethylenediamine (TMED), in t...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2009.07.007
更新日期：2009-11-01 00:00:00
abstract：:Curcumin and Rutin are natural polyphenolic molecules exhibits several pharmacological actives like antibacterial, anticancer, antioxidant, chemo-preventive and anti-inflammatory properties. However till date, no studies have been reported on their combination efficacy, especially in treating multi-drug resistance of ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.06.112
更新日期：2017-11-01 00:00:00
abstract：:Many restoring formulations for damaged hair keratin have been developed. Some patents claim that the hair repair occurs through the reconstruction of disulfide bridges of keratin, through α,β-unsaturated Michael acceptors, such as shikimic acid and bis-aminopropyl diglycol dimaleate. To gain more insights into the po...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.11.194
更新日期：2021-01-15 00:00:00
abstract：:Ultrafine fibers were successfully fabricated from Channel catfish skin-extracted gelatin via electrospinning (ES). Important ES parameters, such as concentration of aqueous acid and fish gelatin solutions, and electric field intensity were examined to investigate the effects on the morphology of the gelatin nanofiber...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2010.06.002
更新日期：2010-10-01 00:00:00
abstract：:Protein phosphorylations have essential regulatory roles in visual signaling. Previously, we found that phosphorylation of several proteins in the retina and retinal pigment epithelium (RPE) is involved in anti-apoptotic signaling under oxidative stress conditions, including light exposure. In this study, we used a ph...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2010.11.006
更新日期：2011-01-01 00:00:00
abstract：:Two kinds of water-insoluble (1-->3)-alpha-D-glucan samples, ab-PCM3-I and ac-PCM3-I, isolated from different Poria cocos mycelia were sulfated, to produce two series of water-soluble derivatives ab-PCM3-I-S1-S5 and ac-PCM3-I-S1-S5, respectively. The derivatives having different weight-average molecular mass (Mw) were...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2004.08.001
更新日期：2004-10-01 00:00:00
abstract：:Annealing processes, involving specific heating protocols, have been used by man for centuries to impart desirable properties to materials - especially metals and particularly tools and weapons. The terminology has also been applied to biopolymers such as starches, where the effects of the processing have been known f...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/s0141-8130(99)00121-x
更新日期：2000-03-16 00:00:00
abstract：:Sequences of 40 very diverse representatives of the alpha-crystallin-small heat-shock protein (alpha-Hsp) superfamily are compared. Their characteristic C-terminal 'alpha-crystallin domain' of 80-100 residues contains short consensus sequences that are highly conserved from prokaryotes to eukaryotes. There are, in add...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/s0141-8130(98)00013-0
更新日期：1998-05-01 00:00:00
abstract：:Neurodegenerative diseases are incongruous, commonly age-related disorders characterized by progressive neuronal loss, comprising the most prevalent being Alzheimer's disease, Parkinson's disease, and Huntington's disease. Perilous health states are anticipated following the neurodegeneration. Their etiology remains l...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2021.01.042
更新日期：2021-01-10 00:00:00
abstract：:Edible films were developed from seaweed polysaccharides (Kappaphycus alvarezii and Ulva fasciata). The total extracted yield of semi refined carrageenan (SRC) and ulvan polysaccharide was 31.55%, and 28.86%, with total carbohydrate being 78.08% and 82.43% in SRC and ulvan polysaccharides. Results of viscometric studi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.02.089
更新日期：2018-06-01 00:00:00
abstract：:The present study aimed at developing edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends. Central composite rotatable design of response surface methodology was used to optimize concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%) for the development of composite ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.09.111
更新日期：2020-01-15 00:00:00
abstract：:Four kinds of astragalus polysaccharides (APSs), APS(t), APS(40), APS(50) and APS(60), were extracted by water decoction and one-step or stepwise ethanol precipitation methods, and modified by chlorosulfonic acid-pyridine method to obtain four sulfated APSs (sAPSs) (sAPS(t), sAPS(40), sAPS(50), sAPS(60)), respectively...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2007.10.019
更新日期：2008-03-01 00:00:00
abstract：:The purpose of this study is to clarify that the amino acid residues (Asp62 and Arg193) are responsible for the activity and stability of arginine kinase (AK). The amino acid residues Asp62 (D62) and Arg193 (R193) are strictly conserved in monomeric AKs and form an ion pair in the transition state analogue complex. In...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2011.05.022
更新日期：2011-10-01 00:00:00
abstract：:The exploration of new methods to produce food packaging with excellent physicochemical and antibacterial properties is of great scientific and technological interest. Here, we successfully prepare the food packaging films that are composed of konjac glucomannan (KGM), poly(ε-caprolactone) (PCL) and silver nanoparticl...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.01.242
更新日期：2020-04-15 00:00:00
abstract：:Due to the limited options for topical management of skin cancer, this study aimed at developing and evaluating nanoemulsions (NE) for topical delivery of the cytotoxic agent piplartine (piperlongumine). NEs were modified with chitosan or sodium alginate, and the effects on the physicochemical properties, piplartine d...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.09.167
更新日期：2020-12-15 00:00:00
abstract：:Chitosan, a naturally occurring biopolymer, has received considerable attention for pharmaceutical applications due to its biocompatible, biodegradable and less toxic properties. In this study, sulfated chitooligosaccharides (COS-S) with different degrees of substitution (DS) were prepared to improve both its water-so...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.09.032
更新日期：2013-01-01 00:00:00
abstract：:An analysis of the possible modes of motion of water molecules in polysaccharide gels indicates that the interpretation of NMR results does not require the supposition of a class of 'bound water' with highly perturbed motional states. There may, however, be water molecules associated with junction zones in the gels wh...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/s0141-8130(97)00045-7
更新日期：1997-08-01 00:00:00
