当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Functional expression and pharmaceutical efficacy of cardiac-specific ion channels in human embryonic stem cell-derived cardiomyocytes.

Functional expression and pharmaceutical efficacy of cardiac-specific ion channels in human embryonic stem cell-derived cardiomyocytes.

Abstract：

:Cardiomyocytes differentiated from human pluripotent stem cells provide promising tools for screening of cardiotoxic drugs. For evaluation of human pluripotent stem cell-derived cardiomyocytes for cardiotoxicity test, in the present study, human embryonic stem cells (hESCs) were differentiated to cardiomyocytes, followed by metabolic selection to enrich the differentiated cardiomyocytes. The highly purified hESC-derived cardiomyocytes (hESC-CMs) expressed several cardiomyocyte-specific markers including cTnT, MLC2a, and α-SA, but not pluripotency markers, such as OCT4 and NANOG. Patch clamp technique and RT-PCR revealed the expression of cardiomyocyte-specific Na+, Ca2+, and K+ channels and cardiac action potential in hESC-CMs. To explore the potential use of hESC-CMs as functional cardiomyocytes for drug discovery and cardiotoxicity screening, we examined the effects of bisindolylmaleimide (BIM) (I), which inhibits native cardiac Ca2+ channels, on the Ca2+ channel activity of hESC-CMs. We observed a similar response for the BIM (I)-induced modulation of Ca2+ channels between hESC-CMs and native cardiomyocytes through L-type Ca2+ channel current. These results suggest that hESC-CMs can be useful for evaluation of pharmaceutical efficacy and safety of novel drug candidate in cardiac research.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Kim HS,Yoon JW,Li H,Jeong GO,Park JJ,Shin SE,Jang IH,Kim JH,Park WS

doi

10.1038/s41598-017-14198-y

subject

Has Abstract

pub_date

2017-10-23 00:00:00

pages

13821

issue

1

issn

2045-2322

pii

10.1038/s41598-017-14198-y

journal_volume

7

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Zolpidem is a potent stoichiometry-selective modulator of α1β3 GABAA receptors: evidence of a novel benzodiazepine site in the α1-α1 interface.

    abstract：:Zolpidem is not a typical GABAA receptor hypnotic. Unlike benzodiazepines, zolpidem modulates tonic GABA currents in the rat dorsal motor nucleus of the vagus, exhibits residual effects in mice lacking the benzodiazepine binding site, and improves speech, cognitive and motor function in human patients with severe brai...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep28674

    authors： Che Has AT,Absalom N,van Nieuwenhuijzen PS,Clarkson AN,Ahring PK,Chebib M

    更新日期：2016-06-27 00:00:00

  • The reunion of two lineages of the Neotropical brown stink bug on soybean lands in the heart of Brazil.

    abstract：:The rapid pace of conversion of natural areas to agricultural systems is highly concerning, and the consequences for conservation and pest management are not yet fully understood. We examined mitochondrial (COI and Cytb) and nuclear (ITS1) gene regions of 21 populations of the stink bug Euschistus heros, to investigat...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-20187-6

    authors： Soares PL,Cordeiro EMG,Santos FNS,Omoto C,Correa AS

    更新日期：2018-02-06 00:00:00

  • Gearing Up the Human Ankle-Foot System to Reduce Energy Cost of Fast Walking.

    abstract：:During locomotion, the human ankle-foot system dynamically alters its gearing, or leverage of the ankle joint on the ground. Shifting ankle-foot gearing regulates speed of plantarflexor (i.e., calf muscle) contraction, which influences economy of force production. Here, we tested the hypothesis that manipulating ankle...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-65626-5

    authors： Ray SF,Takahashi KZ

    更新日期：2020-05-29 00:00:00

  • Regulation of the translation activity of antigen-specific mRNA is responsible for antigen loss and tumor immune escape in a HER2-expressing tumor model.

    abstract：:Tumor cells tend to behave differently in response to immune selective conditions. Contrary to those in therapeutic antitumor conditions, tumor cells in prophylactic antitumor conditions lose antigen expression for antitumor immune escape. Here, using a CT26/HER2 tumor model, we investigate the underlying mechanism(s)...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-39557-9

    authors： Han BS,Ji S,Woo S,Lee JH,Sin JI

    更新日期：2019-02-27 00:00:00

  • Adeno-associated-virus-mediated transduction of the mammary gland enables sustained production of recombinant proteins in milk.

    abstract：:Biopharming for the production of recombinant pharmaceutical proteins in the mammary gland of transgenic animals is an attractive but laborious alternative compared to mammalian cell fermentation. The disadvantage of the lengthy process of genetically modifying an entire animal could be circumvented with somatic trans...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep15115

    authors： Wagner S,Thresher R,Bland R,Laible G

    更新日期：2015-10-14 00:00:00

  • Association Between Vitamin D and Adrenal Parameters with Metabolic and Inflammatory Markers in Polycystic Ovary Syndrome.

    abstract：:Vitamin D deficiency has been related with metabolic alterations in polycystic ovary syndrome (PCOS). As well, hyperactivation of adrenal axis can be programmed early in life and could be related later with PCOS development. Our aim was to establish the relationship between vitamin D and adrenal parameters with metabo...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-40653-z

    authors： Maidana P,Fritzler A,Mocarbel Y,Perez Lana MB,González D,Rosales M,González de Chazal F,Sternberg K,Lamas Majek E,Mallea-Gil S,Genovesi E,Pelayo M,Fabre B,Mesch V

    更新日期：2019-03-08 00:00:00

  • Author Correction: Antibiotic drug-resistance as a complex system driven by socio-economic growth and antibiotic misuse.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-019-50846-1

    authors： Malik B,Bhattacharyya S

    更新日期：2019-10-18 00:00:00

  • Random allocation of pies promotes the evolution of fairness in the Ultimatum Game.

    abstract：:In the Ultimatum Game, two players are offered a chance to win a pie. The proposer suggests how to split the pie. The responder can either accept or reject the deal. If an agreement is not reached, neither player gets anything. Both game theory and evolutionary game theory predict the rational solution that the propos...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep04534

    authors： Wang X,Chen X,Wang L

    更新日期：2014-04-01 00:00:00

  • A New Strategy for Analyzing Time-Series Data Using Dynamic Networks: Identifying Prospective Biomarkers of Hepatocellular Carcinoma.

    abstract：:Time-series metabolomics studies can provide insight into the dynamics of disease development and facilitate the discovery of prospective biomarkers. To improve the performance of early risk identification, a new strategy for analyzing time-series data based on dynamic networks (ATSD-DN) in a systematic time dimension...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep32448

    authors： Huang X,Zeng J,Zhou L,Hu C,Yin P,Lin X

    更新日期：2016-08-31 00:00:00

  • Severe Fever with Thrombocytopenia Syndrome Phlebovirus causes lethal viral hemorrhagic fever in cats.

    abstract：:Severe fever with thrombocytopenia syndrome (SFTS) is an emerging hemorrhagic fever caused by the SFTS phlebovirus (SFTSV). SFTS patients were first reported in China, followed by Japan and South Korea. In 2017, cats were diagnosed with SFTS for the first time, suggesting that these animals are susceptible to SFTSV. T...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-48317-8

    authors： Park ES,Shimojima M,Nagata N,Ami Y,Yoshikawa T,Iwata-Yoshikawa N,Fukushi S,Watanabe S,Kurosu T,Kataoka M,Okutani A,Kimura M,Imaoka K,Hanaki K,Suzuki T,Hasegawa H,Saijo M,Maeda K,Morikawa S

    更新日期：2019-08-19 00:00:00

  • New insight into the residual inactivation of Microcystis aeruginosa by dielectric barrier discharge.

    abstract：:We report the new insight into the dielectric barrier discharge (DBD) induced inactivation of Microcystis aeruginosa, the dominant algae which caused harmful cyanobacterial blooms in many developing countries. In contrast with the previous work, we employed flow cytometry to examine the algal cells, so that we could a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep13683

    authors： Li L,Zhang H,Huang Q

    更新日期：2015-09-08 00:00:00

  • Multiparty Quantum Key Agreement Based on Quantum Search Algorithm.

    abstract：:Quantum key agreement is an important topic that the shared key must be negotiated equally by all participants, and any nontrivial subset of participants cannot fully determine the shared key. To date, the embed modes of subkey in all the previously proposed quantum key agreement protocols are based on either BB84 or ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep45046

    authors： Cao H,Ma W

    更新日期：2017-03-23 00:00:00

  • L290P/V mutations increase ERK3's cytoplasmic localization and migration/invasion-promoting capability in cancer cells.

    abstract：:Protein kinases are frequently mutated in human cancers, which leads to altered signaling pathways and contributes to tumor growth and progression. ERK3 is an atypical mitogen-activated protein kinase (MAPK) containing an S-E-G activation motif rather than the conserved T-X-Y motif in conventional MAPKs such as ERK1/2...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-15135-9

    authors： Alsaran H,Elkhadragy L,Shakya A,Long W

    更新日期：2017-11-03 00:00:00

  • Magnetic metamaterial superlens for increased range wireless power transfer.

    abstract：:The ability to wirelessly power electrical devices is becoming of greater urgency as a component of energy conservation and sustainability efforts. Due to health and safety concerns, most wireless power transfer (WPT) schemes utilize very low frequency, quasi-static, magnetic fields; power transfer occurs via magneto-...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep03642

    authors： Lipworth G,Ensworth J,Seetharam K,Huang D,Lee JS,Schmalenberg P,Nomura T,Reynolds MS,Smith DR,Urzhumov Y

    更新日期：2014-01-10 00:00:00

  • Cicada-inspired cell-instructive nanopatterned arrays.

    abstract：:Biocompatible surfaces hold key to a variety of biomedical problems that are directly related to the competition between host-tissue cell integration and bacterial colonisation. A saving solution to this is seen in the ability of cells to uniquely respond to physical cues on such surfaces thus prompting the search for...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep07122

    authors： Diu T,Faruqui N,Sjöström T,Lamarre B,Jenkinson HF,Su B,Ryadnov MG

    更新日期：2014-11-20 00:00:00

  • Staphylococcal enterotoxins modulate the effector CD4+ T cell response by reshaping the gene expression profile in adults with atopic dermatitis.

    abstract：:Staphylococcus aureus colonizes the skin of atopic dermatitis (AD) individuals, but the impact of its enterotoxins on the chronic activation of CD4+ T cells demands further analysis. We aimed to analyze the CD4+ T cell anergy profile and their phenotypic and functional features through differential expression of cellu...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-49421-5

    authors： Orfali RL,Yoshikawa FSY,Oliveira LMDS,Pereira NZ,de Lima JF,Ramos YÁL,Duarte AJDS,Sato MN,Aoki V

    更新日期：2019-09-11 00:00:00

  • The last Palaeoproteus (Urodela: Batrachosauroididae) of Europe.

    abstract：:The Batrachosauroididae are an enigmatic group of salamanders known from the Cretaceous and Tertiary of North America and Europe. In Europe, the family is known only by two species of the genus Palaeoproteus. The genus has limited distribution in Western and Central Europe. In the present paper, we describe a new spec...

    journal_title：Scientific reports

    pub_type： 历史文章,杂志文章

    doi：10.1038/s41598-020-59255-1

    authors： Vasilyan D,Yanenko V

    更新日期：2020-02-17 00:00:00

  • Publisher Correction: Natural and directed antigenic drift of the H1 influenza virus hemagglutinin stalk domain.

    abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-018-22372-z

    authors： Anderson CS,Ortega S,Chaves FA,Clark AM,Yang H,Topham DJ,DeDiego ML

    更新日期：2018-03-06 00:00:00

  • Dramatic pressure-driven enhancement of bulk skyrmion stability.

    abstract：:The recent discovery of magnetic skyrmion lattices initiated a surge of interest in the scientific community. Several novel phenomena have been shown to emerge from the interaction of conducting electrons with the skyrmion lattice, such as a topological Hall-effect and a spin-transfer torque at ultra-low current densi...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep21347

    authors： Levatić I,Popčević P,Šurija V,Kruchkov A,Berger H,Magrez A,White JS,Rønnow HM,Živković I

    更新日期：2016-02-19 00:00:00

  • CVD graphene/Ge interface: morphological and electronic characterization of ripples.

    abstract：:Graphene grown directly on germanium is a possible route for the integration of graphene into nanoelectronic devices as well as it is of great interest for materials science. The morphology of the interface between graphene and germanium influences the electronic properties and has not already been completely elucidat...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-48998-1

    authors： Mendoza CD,Figueroa NS,Maia da Costa MEH,Freire FL Jr

    更新日期：2019-08-29 00:00:00

  • Noise-induced switching from a symmetry-protected shallow metastable state.

    abstract：:We consider escape from a metastable state of a nonlinear oscillator driven close to triple its eigenfrequency. The oscillator can have three stable states of period-3 vibrations and a zero-amplitude state. Because of the symmetry of period-tripling, the zero-amplitude state remains stable as the driving increases. Ho...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66243-y

    authors： Tadokoro Y,Tanaka H,Dykman MI

    更新日期：2020-06-26 00:00:00

  • Mild synthesis of isoxazoline derivatives via an efficient [4 + 1] annulation reaction of transient nitrosoalkenes and sulfur ylides.

    abstract：:An efficient [4 + 1] annulation between α-bromooximes and sulfur ylides via in situ generation of nitrosoalkenes under mild basic reaction conditions has been developed, providing an expeditious and scalable approach to synthesize biologically interesting isoxazoline derivatives with good to excellent yields. ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-021-81370-w

    authors： Hua TB,Liu CX,Hu WM,Wang L,Yang QQ

    更新日期：2021-01-22 00:00:00

  • De Novo Transcriptome Assembly and Functional Annotation in Five Species of Bats.

    abstract：:High-throughput RNA sequencing is a powerful tool that allows us to perform gene prediction and analyze tissue-specific overexpression of genes, but also at species level comparisons can be performed, although in a more restricted manner. In the present study complete liver transcriptomes of five tropical bat species ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-42560-9

    authors： Moreno-Santillán DD,Machain-Williams C,Hernández-Montes G,Ortega J

    更新日期：2019-04-17 00:00:00

  • Along-shelf connectivity and circumpolar gene flow in Antarctic silverfish (Pleuragramma antarctica).

    abstract：:The Antarctic silverfish (Pleuragramma antarctica) is a critically important forage species with a circumpolar distribution and is unique among other notothenioid species for its wholly pelagic life cycle. Previous studies have provided mixed evidence of population structure over regional and circumpolar scales. The a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-36030-x

    authors： Caccavo JA,Papetti C,Wetjen M,Knust R,Ashford JR,Zane L

    更新日期：2018-12-14 00:00:00

  • Modified VEGF-A mRNA induces sustained multifaceted microvascular response and accelerates diabetic wound healing.

    abstract：:Capable of mediating efficient transfection and protein production without eliciting innate immune responses, chemically modified mRNA holds great potential to produce paracrine factors at a physiologically beneficial level, in a spatiotemporally controlled manner, and with low toxicity. Although highly promising in c...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-35570-6

    authors： Sun N,Ning B,Hansson KM,Bruce AC,Seaman SA,Zhang C,Rikard M,DeRosa CA,Fraser CL,Wågberg M,Fritsche-Danielson R,Wikström J,Chien KR,Lundahl A,Hölttä M,Carlsson LG,Peirce SM,Hu S

    更新日期：2018-11-30 00:00:00

  • Pre-symptomatic modified phytohormone profile is associated with lower phytoplasma titres in an Arabidopsis seor1ko line.

    abstract：:The proteins AtSEOR1 and AtSEOR2 occur as conjugates in the form of filaments in sieve elements of Arabidopsis thaliana. A reduced phytoplasma titre found in infected defective-mutant Atseor1ko plants in previous work raised the speculation that non-conjugated SEOR2 is involved in the phytohormone-mediated suppression...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-71660-0

    authors： Bernardini C,Pagliari L,De Rosa V,Almeida-Trapp M,Santi S,Martini M,Buoso S,Loschi A,Loi N,Chiesa F,Mithöfer A,van Bel AJE,Musetti R

    更新日期：2020-09-08 00:00:00

  • The Dynamics of Nanoparticle-enhanced Fluid Displacement in Porous Media - A Pore-scale Study.

    abstract：:This work provides new insights into the dynamics of silica nanoparticle-based removal of organic fluids (here oil) from naturally occurring porous media. We have used 4D (time-resolved 3D) imaging at pore-scale using X-ray computed micro-tomography (μCT) technique. The captured 3D tomographic time-series data reveal ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-29569-2

    authors： Pak T,Archilha NL,Mantovani IF,Moreira AC,Butler IB

    更新日期：2018-07-24 00:00:00

  • Optimal Multiphase Computed Tomographic Angiography-based Infarct Core Estimations for Acute Ischemic Stroke.

    abstract：:We evaluated the best methods for predicting various infarct core thresholds for endovascular treatment of ischemic stroke using parameters obtained by multiphase computed tomographic angiography (mCTA). Consecutive patients evaluated for endovascular treatment who concomitantly underwent mCTA and stroke magnetic reso...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-51708-6

    authors： Lee SJ,Jung WS,Choi MH,Hong JM,Lee JS,Choi JW

    更新日期：2019-10-23 00:00:00

  • An automated method for the analysis of food intake behaviour in Caenorhabditis elegans.

    abstract：:The study of mechanisms that govern feeding behaviour and its related disorders is a matter of global health interest. The roundworm Caenorhabditis elegans is becoming a model organism of choice to study these conserved pathways. C. elegans feeding depends on the contraction of the pharynx (pumping). Thanks to the wor...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-21964-z

    authors： Rodríguez-Palero MJ,López-Díaz A,Marsac R,Gomes JE,Olmedo M,Artal-Sanz M

    更新日期：2018-02-26 00:00:00

  • Faecal microRNAs as a non-invasive tool in the diagnosis of colonic adenomas and colorectal cancer: A meta-analysis.

    abstract：:MicroRNAs (miRNAs) are proposed as potential biomarkers for the diagnosis of numerous diseases. Here, we performed a meta-analysis to evaluate the utility of faecal miRNAs as a non-invasive tool in colorectal cancer (CRC) screening. A systematic literature search, according to predetermined criteria, in five databases...

    journal_title：Scientific reports

    pub_type： 杂志文章,meta分析

    doi：10.1038/s41598-019-45570-9

    authors： Yau TO,Tang CM,Harriss EK,Dickins B,Polytarchou C

    更新日期：2019-07-01 00:00:00