Modified VEGF-A mRNA induces sustained multifaceted microvascular response and accelerates diabetic wound healing.

Abstract:

:Capable of mediating efficient transfection and protein production without eliciting innate immune responses, chemically modified mRNA holds great potential to produce paracrine factors at a physiologically beneficial level, in a spatiotemporally controlled manner, and with low toxicity. Although highly promising in cardiovascular medicine and wound healing, effects of this emerging therapeutic on the microvasculature and its bioactivity in disease settings remain poorly understood. Here, we longitudinally and comprehensively characterize microvascular responses to AZD8601, a modified mRNA encoding vascular endothelial growth factor A (VEGF-A), in vivo. Using multi-parametric photoacoustic microscopy, we show that intradermal injection of AZD8601 formulated in a biocompatible vehicle results in pronounced, sustained and dose-dependent vasodilation, blood flow upregulation, and neovessel formation, in striking contrast to those induced by recombinant human VEGF-A protein, a non-translatable variant of AZD8601, and citrate/saline vehicle. Moreover, we evaluate the bioactivity of AZD8601 in a mouse model of diabetic wound healing in vivo. Using a boron nanoparticle-based tissue oxygen sensor, we show that sequential dosing of AZD8601 improves vascularization and tissue oxygenation of the wound bed, leading to accelerated re-epithelialization during the early phase of diabetic wound healing.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Sun N,Ning B,Hansson KM,Bruce AC,Seaman SA,Zhang C,Rikard M,DeRosa CA,Fraser CL,Wågberg M,Fritsche-Danielson R,Wikström J,Chien KR,Lundahl A,Hölttä M,Carlsson LG,Peirce SM,Hu S

doi

10.1038/s41598-018-35570-6

subject

Has Abstract

pub_date

2018-11-30 00:00:00

pages

17509

issue

1

issn

2045-2322

pii

10.1038/s41598-018-35570-6

journal_volume

8

pub_type

杂志文章
  • Understanding and mimicking the dual optimality of the fly ear.

    abstract::The fly Ormia ochracea has the remarkable ability, given an eardrum separation of only 520 μm, to pinpoint the 5 kHz chirp of its cricket host. Previous research showed that the two eardrums are mechanically coupled, which amplifies the directional cues. We have now performed a mechanics and optimization analysis whic...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep02489

    authors: Liu H,Currano L,Gee D,Helms T,Yu M

    更新日期:2013-01-01 00:00:00

  • HMBOX1 interacts with MT2A to regulate autophagy and apoptosis in vascular endothelial cells.

    abstract::We previously found that Homeobox containing 1 (HMBOX1) was required for bone mesenchymal stem cell (BMSC) and mouse embryonic stem cell (ESC) differentiation into vascular endothelial cells (VECs). However, the function of HMBOX1 in VECs is still unknown. In this study, we found that HMBOX1 was abundantly expressed i...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep15121

    authors: Ma H,Su L,Yue H,Yin X,Zhao J,Zhang S,Kung H,Xu Z,Miao J

    更新日期:2015-10-12 00:00:00

  • Breast cancer cells exhibits specific dielectric signature in vitro using the open-ended coaxial probe technique from 200 MHz to 13.6 GHz.

    abstract::Here we investigated the feasibility of using microwave spectroscopy for characterization of normal and breast cancer cell lines cultured in vitro. Healthy non-tumorigenic, MCF-10A and breast cancer, MDA-MB-231, Hs578T, T47D and MCF-7 cell lines were electrically characterized using the open-ended coaxial probe techni...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-41124-1

    authors: Hussein M,Awwad F,Jithin D,El Hasasna H,Athamneh K,Iratni R

    更新日期:2019-03-18 00:00:00

  • Protective effect of epigallocatechin-3-gallate (EGCG) on toxic metalloproteinases-mediated skin damage induced by Scyphozoan jellyfish envenomation.

    abstract::Jellyfish stingings are currently raising serious public health concerns around the world. Hence, the search for an effective first aid reagent for the envenomation has been the goal of many investigators in the field. There have been a few previous reports of in vivo as well as in vivo studies suggesting the metallop...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-75269-1

    authors: Hwang DH,Lee H,Choudhary I,Kang C,Chae J,Kim E

    更新日期:2020-10-29 00:00:00

  • A putative chordate luciferase from a cosmopolitan tunicate indicates convergent bioluminescence evolution across phyla.

    abstract::Pyrosomes are tunicates in the phylum Chordata, which also contains vertebrates. Their gigantic blooms play important ecological and biogeochemical roles in oceans. Pyrosoma, meaning "fire-body", derives from their brilliant bioluminescence. The biochemistry of this light production is unknown, but has been hypothesiz...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-73446-w

    authors: Tessler M,Gaffney JP,Oliveira AG,Guarnaccia A,Dobi KC,Gujarati NA,Galbraith M,Mirza JD,Sparks JS,Pieribone VA,Wood RJ,Gruber DF

    更新日期:2020-10-20 00:00:00

  • Risk of active tuberculosis development in contacts exposed to infectious tuberculosis in congregate settings in Korea.

    abstract::Contact investigation is an important and effective active case-finding strategy, but there is a lack of research on congregate settings in countries with an intermediate incidence. This study determined the incidence of and risk factors for tuberculosis (TB) development after exposure in congregate settings. This ret...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-57697-1

    authors: Park SY,Han S,Kim YM,Kim J,Lee S,Yang J,Kim UN,Park MS

    更新日期:2020-01-28 00:00:00

  • Dual-FRET imaging of IP3 and Ca2+ revealed Ca2+-induced IP3 production maintains long lasting Ca2+ oscillations in fertilized mouse eggs.

    abstract::In most species, fertilization induces Ca2+ transients in the egg. In mammals, the Ca2+ rises are triggered by phospholipase Cζ (PLCζ) released from the sperm; IP3 generated by PLCζ induces Ca2+ release from the intracellular Ca2+ store through IP3 receptor, termed IP3-induced Ca2+ release. Here, we developed new fluo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-40931-w

    authors: Matsu-Ura T,Shirakawa H,Suzuki KGN,Miyamoto A,Sugiura K,Michikawa T,Kusumi A,Mikoshiba K

    更新日期:2019-03-18 00:00:00

  • hnRNPK inhibits GSK3β Ser9 phosphorylation, thereby stabilizing c-FLIP and contributes to TRAIL resistance in H1299 lung adenocarcinoma cells.

    abstract::c-FLIP (cellular FLICE-inhibitory protein) is the pivotal regulator of TRAIL resistance in cancer cells, It is a short-lived protein degraded through the ubiquitin/proteasome pathway. The discovery of factors and mechanisms regulating its protein stability is important for the comprehension of TRAIL resistance by tumo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep22999

    authors: Gao X,Feng J,He Y,Xu F,Fan X,Huang W,Xiong H,Liu Q,Liu W,Liu X,Sun X,He QY,Zhang Q,Liu L

    更新日期:2016-03-14 00:00:00

  • Lysing bloom-causing alga Phaeocystis globosa with microbial algicide: An efficient process that decreases the toxicity of algal exudates.

    abstract::Algicidal microbes could effectively remove the harmful algae from the waters. In this study, we were concerned with the ecological influence of an algicide extracted from Streptomyces alboflavus RPS, which could completely lyse the Phaeocystis globosa cells within two days. In microcosms, 4 μg/mL of the microbial alg...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep20081

    authors: Cai G,Yang X,Lai Q,Yu X,Zhang H,Li Y,Chen Z,Lei X,Zheng W,Xu H,Zheng T

    更新日期:2016-02-05 00:00:00

  • Bi-specific molecule against EGFR and death receptors simultaneously targets proliferation and death pathways in tumors.

    abstract::Developing therapeutics that target multiple receptor signaling pathways in tumors is critical as therapies targeting single specific biomarker/pathway have shown limited efficacy in patients with cancer. In this study, we extensively characterized a bi-functional molecule comprising of epidermal growth factor recepto...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-02483-9

    authors: Zhu Y,Bassoff N,Reinshagen C,Bhere D,Nowicki MO,Lawler SE,Roux J,Shah K

    更新日期:2017-06-01 00:00:00

  • The northern limit of corals of the genus Acropora in temperate zones is determined by their resilience to cold bleaching.

    abstract::The distribution of corals in Japan covers a wide range of latitudes, encompassing tropical to temperate zones. However, coral communities in temperate zones contain only a small subset of species. Among the parameters that determine the distribution of corals, temperature plays an important role. We tested the resili...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep18467

    authors: Higuchi T,Agostini S,Casareto BE,Suzuki Y,Yuyama I

    更新日期:2015-12-18 00:00:00

  • Machine learning identifies a strong association between warming and reduced primary productivity in an oligotrophic ocean gyre.

    abstract::Phytoplankton play key roles in the oceans by regulating global biogeochemical cycles and production in marine food webs. Global warming is thought to affect phytoplankton production both directly, by impacting their photosynthetic metabolism, and indirectly by modifying the physical environment in which they grow. In...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-59989-y

    authors: D'Alelio D,Rampone S,Cusano LM,Morfino V,Russo L,Sanseverino N,Cloern JE,Lomas MW

    更新日期:2020-02-25 00:00:00

  • Optimal Environmental Conditions and Anomalous Ecosystem Responses: Constraining Bottom-up Controls of Phytoplankton Biomass in the California Current System.

    abstract::In Eastern Boundary Current systems, wind-driven upwelling drives nutrient-rich water to the ocean surface, making these regions among the most productive on Earth. Regulation of productivity by changing wind and/or nutrient conditions can dramatically impact ecosystem functioning, though the mechanisms are not well u...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27612

    authors: Jacox MG,Hazen EL,Bograd SJ

    更新日期:2016-06-09 00:00:00

  • A mesophotic black coral forest in the Adriatic Sea.

    abstract::A forest of the black coral Antipathella subpinnata was found from 52 to 80 m depth in three different sites at Tremiti Islands Marine Protected Area (MPA; Mediterranean Sea), with two of them hosting a monospecific forest on horizontal and vertical substrates. Colonies of A. subpinnata showed a mean density between 0...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-65266-9

    authors: Chimienti G,De Padova D,Mossa M,Mastrototaro F

    更新日期:2020-05-22 00:00:00

  • Distribution Analysis via Mass Spectrometry Imaging of Ephedrine in the Lungs of Rats Orally Administered the Japanese Kampo Medicine Maoto.

    abstract::Maoto, a traditional Japanese Kampo medicine, has been used to treat various respiratory diseases, including respiratory infections and influenza. Ephedrine (EPD), the main ingredient in maoto, is also clinically used to treat respiratory diseases. However, the pharmacokinetics and distribution of EPD in the lungs aft...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep44098

    authors: Matsumoto T,Kushida H,Matsushita S,Oyama Y,Suda T,Watanabe J,Kase Y,Setou M

    更新日期:2017-03-08 00:00:00

  • Comprehensive profiling of JMJD3 in gastric cancer and its influence on patient survival.

    abstract::Histone methylation is thought to control the regulation of genetic program and the dysregulation of it has been found to be closely associated with cancer. JMJD3 has been identified as an H3K27 demethylase and its role in cancer development is context specific. The role of JMJD3 in gastric cancer (GC) has not been ex...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-37340-w

    authors: Xu Z,Xia Y,Xiao Z,Jia Y,Li L,Jin Y,Zhao Q,Wan L,Yi T,Yu Y,Wen Q,Zhu Y,Qin B,Zhang F,Shen J

    更新日期:2019-01-29 00:00:00

  • Generation of westerly wind bursts by forcing outside the tropics.

    abstract::The westerly wind burst (WWB) is an important triggering mechanism of El Niño and typically occurs in the western Pacific Ocean. The Fourier spectrum of the wind field over the western tropical Pacific is characterised by a large variety of peaks distributed from intra-seasonal to decadal time scales, suggesting that ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-79655-7

    authors: Sullivan A,Zhong W,Borzelli GLE,Geng T,Mackallah C,Ng B,Hong CC,Cai W,Huang AY,Bodman R

    更新日期:2021-01-13 00:00:00

  • Impact of Comorbidities and Smoking on the Outcome in Aneurysmal Subarachnoid Hemorrhage.

    abstract::The intention of this observational study is to show the significant impact of comorbidities and smoking on the outcome in aneurysmal subarachnoid hemorrhage (SAH). During this observational study 203 cases of treatment of ruptured intracranial aneurysms were analyzed. We examined and classified prospectively the 12 m...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-30878-9

    authors: Hammer A,Steiner A,Ranaie G,Yakubov E,Erbguth F,Hammer CM,Killer-Oberpfalzer M,Steiner H,Janssen H

    更新日期:2018-08-17 00:00:00

  • DoGMP1 from Dendrobium officinale contributes to mannose content of water-soluble polysaccharides and plays a role in salt stress response.

    abstract::GDP-mannose pyrophosphorylase (GMP) catalyzed the formation of GDP-mannose, which serves as a donor for the biosynthesis of mannose-containing polysaccharides. In this study, three GMP genes from Dendrobium officinale (i.e., DoGMPs) were cloned and analyzed. The putative 1000 bp upstream regulatory region of these DoG...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep41010

    authors: He C,Yu Z,Teixeira da Silva JA,Zhang J,Liu X,Wang X,Zhang X,Zeng S,Wu K,Tan J,Ma G,Luo J,Duan J

    更新日期:2017-02-08 00:00:00

  • Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions.

    abstract::Multiphoton excitation microscopy (MPM) is regarded as an effective tool that enables the visualization of deep regions within living tissues and organs, with little damage. Here, we report novel non-labeling MPM (NL-MPM) imaging of fresh human colorectal mucosa, which is useful for discriminating cancer lesions from ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07244-2

    authors: Matsui T,Mizuno H,Sudo T,Kikuta J,Haraguchi N,Ikeda JI,Mizushima T,Yamamoto H,Morii E,Mori M,Ishii M

    更新日期:2017-07-31 00:00:00

  • Metabolic and transcriptional alternations for defense by interfering OsWRKY62 and OsWRKY76 transcriptions in rice.

    abstract::Metabolomic and transcriptomic approaches were used to dissect the enhanced disease resistance in the plants harbouring a RNA interfering construct of OsWRKY62 and OsWRKY76 (dsOW62/76) genes. The primary metabolic pathways were activated in dsOW62/76 compared with wild-type (ZH17) plants, revealed by increased accumul...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-02643-x

    authors: Liang X,Chen X,Li C,Fan J,Guo Z

    更新日期:2017-05-30 00:00:00

  • SNG and DNG meta-absorber with fractional absorption band for sensing application.

    abstract::This paper reports on a tunable transmission frequency characteristics-based metamaterial absorber of an X band sensing application with a fractional bandwidth. Tunable resonator metamaterial absorbers fabricated with dielectric surface have been the subject of growing attention of late. Absorbers possess electromagne...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-69792-4

    authors: Hoque A,Islam MT,Almutairi AF,Chowdhury MEH,Samsuzzaman M

    更新日期:2020-08-04 00:00:00

  • Microplastic contamination of table salts from Taiwan, including a global review.

    abstract::Plastic pollution is a rapidly worsening environmental problem, especially in oceanic habitats. Environmental pollution with microplastic particles is also causing food consumed by humans to be increasingly polluted, including table salts. Therefore, we present the first study which focuses only on table salt products...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-46417-z

    authors: Lee H,Kunz A,Shim WJ,Walther BA

    更新日期:2019-07-12 00:00:00

  • Identification and Structural Characterization of the N-terminal Amyloid Core of Orb2 isoform A.

    abstract::Orb2 is a functional amyloid that plays a key role in Drosophila long-term memory formation. Orb2 has two isoforms that differ in their N-termini. The N-terminus of the A isoform (Orb2A) that precedes its Q-rich prion-like domain has been shown to be important for Orb2 aggregation and long-term memory. However, beside...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep38265

    authors: Cervantes SA,Bajakian TH,Soria MA,Falk AS,Service RJ,Langen R,Siemer AB

    更新日期:2016-12-06 00:00:00

  • Long non-coding RNA PARTICLE bridges histone and DNA methylation.

    abstract::PARTICLE (Gene PARTICL- 'Promoter of MAT2A-Antisense RadiaTion Induced Circulating LncRNA) expression is transiently elevated following low dose irradiation typically encountered in the workplace and from natural sources. This long non-coding RNA recruits epigenetic silencers for cis-acting repression of its neighbour...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-01875-1

    authors: O'Leary VB,Hain S,Maugg D,Smida J,Azimzadeh O,Tapio S,Ovsepian SV,Atkinson MJ

    更新日期:2017-05-11 00:00:00

  • Associations of PRKN-PACRG SNPs and G × G and G × E interactions with the risk of hyperlipidaemia.

    abstract::This research aimed to assess the associations of 7 parkin RBR E3 ubiquitin protein ligase (PRKN) and 4 parkin coregulated gene (PACRG) single-nucleotide polymorphisms (SNPs), their haplotypes, gene-gene (G × G) and gene-environment (G × E) interactions with hyperlipidaemia in the Chinese Maonan minority. The genotype...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-68826-1

    authors: Zheng PF,Yin RX,Wei BL,Liu CX,Deng GX,Guan YZ

    更新日期:2020-08-03 00:00:00

  • A New Fitting Method for Ambipolar Diffusion Length Extraction in Thin Film Structures Using Photoluminescence Measurement with Scanning Excitation.

    abstract::A new simple method is proposed to extract the ambipolar diffusion length for two-dimensional (2D) electronic transport in thin film structures using a scanning photoluminescence microscopy (SPLM) setup. No spatially-resolved photoluminescence detection methods are required. By measuring the excitation-position-depend...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-62093-w

    authors: Chu CH,Mao MH,Lin YR,Lin HH

    更新日期:2020-03-23 00:00:00

  • Variations in plantar pressure and balance in HIV-infected children in antiretroviral therapy.

    abstract::Balance disorders have been poorly investigated and somewhat neglected in people infected with the human immunodeficiency virus, especially in children, whose have intrinsic and extrinsic risk factors that may compromise the balance. To evaluate the foot plantar pressures and the balance in children with acquired immu...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-41028-0

    authors: da Silva Pontes L,Callegari B,Magno L,Moraes A,Silva BG,Manso K,Barros B,Araújo AP,Silva MC,Dias GA,Vasconcelos BH,Costa E Silva A,Libonati RM,Souza GS

    更新日期:2019-03-13 00:00:00

  • Evolutionary dynamics of the chloroplast genome sequences of six Colobanthus species.

    abstract::The complete plastome sequences of six species were sequenced to better understand the evolutionary relationships and mutation patterns in the chloroplast genome of the genus Colobanthus. The length of the chloroplast genome sequences of C. acicularis, C. affinis, C. lycopodioides, C. nivicola, C. pulvinatus and C. su...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-68563-5

    authors: Androsiuk P,Jastrzębski JP,Paukszto Ł,Makowczenko K,Okorski A,Pszczółkowska A,Chwedorzewska KJ,Górecki R,Giełwanowska I

    更新日期:2020-07-13 00:00:00

  • Experimental observation of weak non-Markovianity.

    abstract::Non-Markovianity has recently attracted large interest due to significant advances in its characterization and its exploitation for quantum information processing. However, up to now, only non-Markovian regimes featuring environment to system backflow of information (strong non-Markovianity) have been experimentally s...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep17520

    authors: Bernardes NK,Cuevas A,Orieux A,Monken CH,Mataloni P,Sciarrino F,Santos MF

    更新日期:2015-12-02 00:00:00