Abstract:
:Monosynaptic excitatory connections between cerebellar granule and Purkinje cells were studied in dissociated cell cultures, and identification of the transmitter and the postsynaptic receptor at this synapse was pharmacologically investigated. The presynaptic granule cell and the postsynaptic Purkinje cell were voltage- or current-clamped simultaneously, and the excitatory postsynaptic current induced by the granule cell was examined. The neurons and monosynaptic excitatory connections were identified as in our earlier study. Several pairs of granule and Purkinje cells were stained with Lucifer yellow and propidium iodide, respectively, and their morphology was examined after electrophysiological recording. The monosynaptic excitatory postsynaptic current was suppressed by 1 mM kynurenate, an antagonist for excitatory-amino acid receptors, but was little affected by 0.2 mM DL-2-amino-5-phosphonovalerate, a selective antagonist of N-methyl-D-aspartate receptors. Glutamate and aspartate induced inward current in the Purkinje cells. These currents were suppressed by kynurenate at 1 mM. DL-2-Amino-5-phosphonovalerate at 0.2 mM suppressed the inward current induced by 100 microM aspartate but did not affect the inward current induced by 10 microM glutamate. These results are consistent with the idea that glutamate, or a glutamate-like substance, but not aspartate is the transmitter released at the synapse between granule and Purkinje cells and that non-N-methyl-D-aspartate receptor channels are functioning in the postsynaptic membrane.
journal_name
Proc Natl Acad Sci U S Aauthors
Hirano T,Hagiwara Sdoi
10.1073/pnas.85.3.934subject
Has Abstractpub_date
1988-02-01 00:00:00pages
934-8issue
3eissn
0027-8424issn
1091-6490journal_volume
85pub_type
杂志文章abstract::Oriented cell division is critical for cell fate specification, tissue organization, and tissue homeostasis, and relies on proper orientation of the mitotic spindle. The molecular mechanisms underlying the regulation of spindle orientation remain largely unknown. Herein, we identify a critical role for cylindromatosis...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
doi:10.1073/pnas.1319341111
更新日期:2014-02-11 00:00:00
abstract::Two F' plasmids isolated independently from a recA- strain of Escherichia coli and containing identical deletion end points and identical associated inverted duplications are described. In these plasmids, DNA of F plasmid from the IS3 element alpha 1 beta 1 up to the transposon gamma delta is duplicated in inverted or...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
doi:10.1073/pnas.81.15.4869
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abstract::Mutant alleles at the dilute unconventional myosin heavy chain locus cause diluted coat color, opisthotonic seizures, and death. The dilute coat color phenotype is caused by irregular clumping of pigment in the hair, but amounts of melanin are unchanged from wild-type controls. The melanocyte phenotype has been descri...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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doi:10.1073/pnas.93.25.14554
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abstract::Despite significant infiltration into tumors and atherosclerotic plaques, the role of T lymphocytes in these pathological conditions is still unclear. We have demonstrated that tumor-infiltrating lymphocytes (TILs) and plaque-infiltrating lymphocytes (PILs) produce heparin-binding epidermal growth factor-like growth f...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
doi:10.1073/pnas.92.14.6547
更新日期:1995-07-03 00:00:00
abstract::Antibodies from three different lines of monoclonal hybridomas crossreact with both the beta subunit of spectrin and the 90-kDa peptide present in highly purified preparations of the heme-controlled eIF-2 alpha (initiation factor 2 alpha-subunit) kinase from rabbit reticulocytes. Antibodies from two of the three lines...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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doi:10.1073/pnas.82.16.5332
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abstract::We have recently purified calorigen, the natural trigger for heat production in the inflorescences of Sauromatum guttatum Schott (voodoo lily), a thermogenic plant, and identified it as salicylic acid. Since then an analytical assay was developed that allows the quantitation of salicylic acid in plant tissues. This as...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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doi:10.1073/pnas.86.7.2214
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abstract::Engagement of high-affinity IgE receptors leads to activation of tyrosine and serine/threonine kinases and the immediate phosphorylation of receptor beta (serine and tyrosine) and gamma (threonine and tyrosine) chains. Receptor disengagement leads to dephosphorylation of beta and gamma chains via the action of undefin...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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abstract::Children who are treated for congenital cataracts later exhibit impairments in configural face analysis. This has been explained in terms of a critical period for the acquisition of normal face processing. Here, we consider a more parsimonious account according to which deficits in configural analysis result from the ...
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2014-08-12 00:00:00
abstract::Identifying the drivers and processes that determine globally the geographic range size of species is crucial to understanding the geographic distribution of biodiversity and further predicting the response of species to current global changes. However, these drivers and processes are still poorly understood, and no e...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
doi:10.1073/pnas.1902484116
更新日期:2019-07-02 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
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更新日期:2012-05-08 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::Genetic variation in the serotonin transporter gene (SLC6A4) is associated with vulnerability to affective disorders and pharmacotherapy efficacy. We recently identified sequence polymorphisms in the common marmoset SLC6A4 repeat region (AC/C/G and CT/T/C) associated with individual differences in anxiety-like trait, ...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2010-09-21 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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doi:10.1073/pnas.94.16.8321
更新日期:1997-08-05 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1995-02-14 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
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更新日期:2016-07-19 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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doi:10.1073/pnas.75.11.5501
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2013-02-05 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
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更新日期:2013-12-17 00:00:00
abstract::Long interspersed nuclear elements 1 (L1) are active retrotransposons that reside in many species, including humans and rodents. L1 elements produce an RNA intermediate that is reverse transcribed to DNA and inserted in a new genomic location. We have tagged an active human L1 element (L1(RP)) with a gene encoding enh...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2003-02-18 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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doi:10.1073/pnas.91.5.1883
更新日期:1994-03-01 00:00:00