当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES期刊下所有文献 > In Vitro Glucuronidation of Wushanicaritin by Liver Microsomes, Intestine Microsomes and Expressed Human UDP-Glucuronosyltransferase Enzymes.

In Vitro Glucuronidation of Wushanicaritin by Liver Microsomes, Intestine Microsomes and Expressed Human UDP-Glucuronosyltransferase Enzymes.

Abstract：

:Wushanicaritin, a natural polyphenol compound, exerts many biological activities. This study aimed to characterize wushanicaritin glucuronidation by pooled human liver microsomes (HLM), human intestine microsomes and individual uridine diphosphate-glucuronosyltransferase (UGT) enzyme. Glucuronidation rates were determined by incubating wushanicaritin with uridine diphosphoglucuronic acid-supplemented microsomes. Kinetic parameters were derived by appropriate model fitting. Reaction phenotyping, the relative activity factor (RAF) and activity correlation analysis were performed to identify the main UGT isoforms. Wushanicaritin glucuronidation in HLM was efficient with a high CLint (intrinsic clearance) value of 1.25 and 0.69 mL/min/mg for G1 and G2, respectively. UGT1A1 and 1A7 showed the highest activities with the intrinsic clearance (CLint) values of 1.16 and 0.38 mL/min/mg for G1 and G2, respectively. In addition, G1 was significantly correlated with β-estradiol glucuronidation (r = 0.847; p = 0.0005), while G2 was also correlated with chenodeoxycholic acid glucuronidation (r = 0.638, p = 0.026) in a bank of individual HLMs (n = 12). Based on the RAF approach, UGT1A1 contributed 51.2% for G1, and UGT1A3 contributed 26.0% for G2 in HLM. Moreover, glucuronidation of wushanicaritin by liver microsomes showed marked species difference. Taken together, UGT1A1, 1A3, 1A7, 1A8, 1A9 and 2B7 were identified as the main UGT contributors responsible for wushanicaritin glucuronidation.

journal_name

Int J Mol Sci

journal_title

International journal of molecular sciences

authors

Hong X,Zheng Y,Qin Z,Wu B,Dai Y,Gao H,Yao Z,Gonzalez FJ,Yao X

doi

10.3390/ijms18091983

subject

Has Abstract

pub_date

2017-09-19 00:00:00

issue

9

issn

1422-0067

pii

ijms18091983

journal_volume

18

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES文献大全
  • Novel Peptide-Based Inhibitors for Microtubule Polymerization in Phytophthora capsici.

    abstract：:The plant disease Phytophthora blight, caused by the oomycete pathogen Phytophthora capsici, is responsible for major economic losses in pepper production. Microtubules have been an attractive target for many antifungal agents as they are involved in key cellular events such as cell proliferation, signaling, and migra...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20112641

    authors： Lee SC,Kim SH,Hoffmeister RA,Yoon MY,Kim SK

    更新日期：2019-05-29 00:00:00

  • Defect of Interferon γ Leads to Impaired Wound Healing through Prolonged Neutrophilic Inflammatory Response and Enhanced MMP-2 Activation.

    abstract：:Interferon (IFN)-γ is mainly secreted by CD4+ T helper 1 (Th1), natural killer (NK) and NKT cells after skin injury. Although IFN-γ is well known regarding its inhibitory effects on collagen synthesis by fibroblasts in vitro, information is limited regarding its role in wound healing in vivo. In the present study, we ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20225657

    authors： Kanno E,Tanno H,Masaki A,Sasaki A,Sato N,Goto M,Shisai M,Yamaguchi K,Takagi N,Shoji M,Kitai Y,Sato K,Kasamatsu J,Ishii K,Miyasaka T,Kawakami K,Imai Y,Iwakura Y,Maruyama R,Tachi M,Kawakami K

    更新日期：2019-11-12 00:00:00

  • Quantitative Structure-Activity Relationships Study on the Rate Constants of Polychlorinated Dibenzo-p-Dioxins with OH Radical.

    abstract：:The OH-initiated reaction rate constants (kOH) are of great importance to measure atmospheric behaviors of polychlorinated dibenzo-p-dioxins (PCDDs) in the environment. The rate constants of 75 PCDDs with the OH radical at 298.15 K have been calculated using high level molecular orbital theory, and the rate constants ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms160818812

    authors： Qi C,Zhang C,Sun X

    更新日期：2015-08-12 00:00:00

  • Targeting the Ubiquitin Signaling Cascade in Tumor Microenvironment for Cancer Therapy.

    abstract：:Tumor microenvironments are composed of a myriad of elements, both cellular (immune cells, cancer-associated fibroblasts, mesenchymal stem cells, etc.) and non-cellular (extracellular matrix, cytokines, growth factors, etc.), which collectively provide a permissive environment enabling tumor progression. In this revie...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms22020791

    authors： Liu Q,Aminu B,Roscow O,Zhang W

    更新日期：2021-01-14 00:00:00

  • Phylogenetic, Molecular, and Functional Characterization of PpyCBF Proteins in Asian Pears (Pyrus pyrifolia).

    abstract：:C-repeat binding factor/dehydration-responsive element (CBF/DRE) transcription factors (TFs) participate in a variety of adaptive mechanisms, and are involved in molecular signaling and abiotic stress tolerance in plants. In pear (Pyrus pyrifolia) and other rosaceous crops, the independent evolution of CBF subfamily m...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20092074

    authors： Ahmad M,Li J,Yang Q,Jamil W,Teng Y,Bai S

    更新日期：2019-04-26 00:00:00

  • Role of Forkhead Box O Transcription Factors in Oxidative Stress-Induced Chondrocyte Dysfunction: Possible Therapeutic Target for Osteoarthritis?

    abstract：:Chondrocyte dysfunction occurs during the development of osteoarthritis (OA), typically resulting from a deleterious increase in oxidative stress. Accordingly, strategies for arresting oxidative stress-induced chondrocyte dysfunction may lead to new potential therapeutic targets for OA treatment. Forkhead box O (FoxO)...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms19123794

    authors： Wang R,Zhang S,Previn R,Chen D,Jin Y,Zhou G

    更新日期：2018-11-28 00:00:00

  • Synergistic Interaction of Phytohormones in Determining Leaf Angle in Crops.

    abstract：:Leaf angle (LA), defined as the angle between the plant stem and leaf adaxial side of the blade, generally shapes the plant architecture into a loosen or dense structure, and thus influences the light interception and competition between neighboring plants in natural settings, ultimately contributing to the crop yield...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21145052

    authors： Li X,Wu P,Lu Y,Guo S,Zhong Z,Shen R,Xie Q

    更新日期：2020-07-17 00:00:00

  • The Impact of Single- and Double-Strand DNA Breaks in Human Spermatozoa on Assisted Reproduction.

    abstract：:Several cellular insults can result in sperm DNA fragmentation either on one or both DNA strands. Oxidative damage, premature interruption of the apoptotic process and defects in DNA compaction during spermatogenesis are the main mechanisms that cause DNA breaks in sperm. The two-tailed Comet assay is the only techniq...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21113882

    authors： Agarwal A,Barbăroșie C,Ambar R,Finelli R

    更新日期：2020-05-29 00:00:00

  • Influence of Hypoxic and Hyperoxic Preconditioning on Endothelial Function in a Model of Myocardial Ischemia-Reperfusion Injury with Cardiopulmonary Bypass (Experimental Study).

    abstract：:The aim of the experiment was to evaluate the effect of preconditioning based on changes in inspiratory oxygen fraction on endothelial function in the model of ischemia-reperfusion injury of the myocardium in the condition of cardiopulmonary bypass. The prospective randomized study included 32 rabbits divided into fou...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21155336

    authors： Mandel IA,Podoksenov YK,Suhodolo IV,An DA,Mikheev SL,Podoksenov AY,Svirko YS,Gusakova AM,Shipulin VM,Yavorovskiy AG

    更新日期：2020-07-27 00:00:00

  • Defective Regulation of Membrane TNFα Expression in Dendritic Cells of Glioblastoma Patients Leads to the Impairment of Cytotoxic Activity against Autologous Tumor Cells.

    abstract：:Besides an antigen-presenting function and ability to induce antitumor immune responses, dendritic cells (DCs) possess a direct tumoricidal activity. We previously reported that monocyte-derived IFNα-induced DCs (IFN-DCs) of glioblastoma multiforme patients express low levels of membrane TNFα molecule (mTNFα) and have...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21082898

    authors： Tyrinova T,Leplina O,Mishinov S,Tikhonova M,Dolgova E,Proskurina A,Stupack V,Bogachev S,Ostanin A,Chernykh E

    更新日期：2020-04-21 00:00:00

  • Identification of Proteins Modulated in the Date Palm Stem Infested with Red Palm Weevil (Rhynchophorus ferrugineus Oliv.) Using Two Dimensional Differential Gel Electrophoresis and Mass Spectrometry.

    abstract：:A state of the art proteomic methodology using Matrix Assisted Laser Desorption/Ionization-Time of Flight (MALDI TOF) has been employed to characterize peptides modulated in the date palm stem subsequent to infestation with red palm weevil (RPW). Our analyses revealed 32 differentially expressed peptides associated wi...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms160819326

    authors： Rasool KG,Khan MA,Aldawood AS,Tufail M,Mukhtar M,Takeda M

    更新日期：2015-08-17 00:00:00

  • Influence of Cationic meso-Substituted Porphyrins on the Antimicrobial Photodynamic Efficacy and Cell Membrane Interaction in Escherichia coli.

    abstract：:Photodynamic inactivation (PDI) is a non-antibiotic option for the treatment of infectious diseases. Although Gram-positive bacteria have been shown to be highly susceptible to PDI, the inactivation of Gram-negative bacteria has been more challenging due to the impermeability properties of the outer membrane. In the p...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20010134

    authors： Hurst AN,Scarbrough B,Saleh R,Hovey J,Ari F,Goyal S,Chi RJ,Troutman JM,Vivero-Escoto JL

    更新日期：2019-01-01 00:00:00

  • Participation of NADPH Oxidase-Related Reactive Oxygen Species in Leptin-Promoted Pulmonary Inflammation: Regulation of cPLA2α and COX-2 Expression.

    abstract：:Obesity is a worldwide epidemic problem and correlates to varieties of acute or chronic lung diseases such as acute respiratory distress syndrome, chronic obstructive pulmonary disease, and pulmonary fibrosis. An increase of leptin, a kind of adipokine, in lean mice plasma has been found to impair immune responses and...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20051078

    authors： Hsu PS,Lin CM,Chang JF,Wu CS,Sia KC,Lee IT,Huang KY,Lin WN

    更新日期：2019-03-02 00:00:00

  • Novel Bacterial Topoisomerase Inhibitors Exploit Asp83 and the Intrinsic Flexibility of the DNA Gyrase Binding Site.

    abstract：:DNA gyrases are enzymes that control the topology of DNA in bacteria cells. This is a vital function for bacteria. For this reason, DNA gyrases are targeted by widely used antibiotics such as quinolones. Recently, structural and biochemical investigations identified a new class of DNA gyrase inhibitors called NBTIs (i...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms19020453

    authors： Franco-Ulloa S,La Sala G,Miscione GP,De Vivo M

    更新日期：2018-02-03 00:00:00

  • Terminal Phase Components of the Clotting Cascade in Patients with End-Stage Renal Disease Undergoing Hemodiafiltration or Hemodialysis Treatment.

    abstract：:Hemostasis disorder in patients with end-stage renal disease (ESRD) is frequently associated with bleeding diathesis but it may also manifest in thrombotic complications. Analysis of individual coagulation and fibrinolytic factors may shed light on the background of this paradox situation. Here we explored components ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21228426

    authors： Pénzes K,Hurják B,Katona É,Becs G,Balla J,Muszbek L

    更新日期：2020-11-10 00:00:00

  • The Emerging Role of LHb CaMKII in the Comorbidity of Depressive and Alcohol Use Disorders.

    abstract：:Depressive disorders and alcohol use disorders are widespread among the general population and are significant public health and economic burdens. Alcohol use disorders often co-occur with other psychiatric conditions and this dual diagnosis is called comorbidity. Depressive disorders invariably contribute to the deve...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21218123

    authors： Shor C,Zuo W,Eloy JD,Ye JH

    更新日期：2020-10-30 00:00:00

  • Role of mTOR Inhibitors in Kidney Disease.

    abstract：:The first compound that inhibited the mammalian target of rapamycin (mTOR), sirolimus (rapamycin) was discovered in the 1970s as a soil bacterium metabolite collected on Easter Island (Rapa Nui). Because sirolimus showed antiproliferative activity, researchers investigated its molecular target and identified the TOR1 ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms17060975

    authors： Kajiwara M,Masuda S

    更新日期：2016-06-21 00:00:00

  • Age, Diet and Epidermal Signaling Modulate Dermal Fibroblasts' Adipogenic Potential.

    abstract：:The recognition of a distinct fat depot, the dermal white adipose tissue (dWAT), points out the complexity of the interaction among skin resident cells: keratinocytes, dermal fibroblasts (DFs) and adipocytes in response to physiological (diet, age) and pathological (injury) stimulations. dWAT has been recognized as a ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21238955

    authors： Walendzik K,Bukowska J,Kopcewicz M,Machcinska S,Gimble JM,Gawronska-Kozak B

    更新日期：2020-11-25 00:00:00

  • Computational Characterization of Small Molecules Binding to the Human XPF Active Site and Virtual Screening to Identify Potential New DNA Repair Inhibitors Targeting the ERCC1-XPF Endonuclease.

    abstract：:The DNA excision repair protein ERCC-1-DNA repair endonuclease XPF (ERCC1-XPF) is a heterodimeric endonuclease essential for the nucleotide excision repair (NER) DNA repair pathway. Although its activity is required to maintain genome integrity in healthy cells, ERCC1-XPF can counteract the effect of DNA-damaging ther...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms19051328

    authors： Gentile F,Barakat KH,Tuszynski JA

    更新日期：2018-04-30 00:00:00

  • ceRNA Cross-Talk in Paulownia Witches' Broom Disease.

    abstract：:Long noncoding RNA (lncRNA), circular RNA (circRNA), and microRNA (miRNA) are important in the regulation of life activities. However, their function is unclear in Paulownia fortunei. To identify lncRNAs, circRNAs, and miRNA, and investigate their roles in the infection progress of Paulownia witches' broom (PaWB) dise...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms19082463

    authors： Fan G,Wang Z,Zhai X,Cao Y

    更新日期：2018-08-20 00:00:00

  • Serum Matrix Metalloproteinases and Left Atrial Remodeling-The Hoorn Study.

    abstract：:Extracellular matrix protein turnover may play an important role in left atrial (LA) remodelling. The aim is to investigate the associations between matrix metalloproteinase (MMPs), tissue inhibitor of metalloproteinase (TIMP-1) and LA volume index (LAVI) and if these associations are independent of TIMP-1 levels. Par...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21144944

    authors： Linssen PBC,Brunner-La Rocca HP,Schalkwijk CG,Beulens JWJ,Elders PJM,van der Heijden AA,Slieker RC,Stehouwer CDA,Henry RMA

    更新日期：2020-07-13 00:00:00

  • Differences in Liver TFAM Binding to mtDNA and mtDNA Damage between Aged and Extremely Aged Rats.

    abstract：:While mitochondrial dysfunction is acknowledged as a major feature of aging, much less is known about the role of mitochondria in extended longevity. Livers from aged (28-month-old) and extremely aged (32-month-old) rats were analyzed for citrate synthase activity, mitochondrial transcription factor A (TFAM) amount, m...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20102601

    authors： Chimienti G,Picca A,Fracasso F,Marzetti E,Calvani R,Leeuwenburgh C,Russo F,Lezza AMS,Pesce V

    更新日期：2019-05-27 00:00:00

  • Metabolomic and Transcriptomic Profiling Provide Novel Insights into Fruit Ripening and Ripening Disorder Caused by 1-MCP Treatments in Papaya.

    abstract：:Treatment with 1-methylcyclopropylene (1-MCP) is an effective technique to preserve fruits, but inappropriate treatment with 1-MCP causes a ripening disorder (rubbery texture) in papaya fruit. In this study, a combined metabolomic and transcriptomic analysis was conducted to reveal the possible mechanism of the ripeni...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms22020916

    authors： Zheng S,Hao Y,Fan S,Cai J,Chen W,Li X,Zhu X

    更新日期：2021-01-18 00:00:00

  • Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus.

    abstract：:The Kv4 family of voltage-gated K⁺ channels underlie the fast transient (A-type) outward K⁺ current. Although A-type currents are critical to determine somato-dendritic integration in central neurons, relatively little is known about the precise subcellular localisation of the underlying channels in hippocampal circui...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20020246

    authors： Alfaro-Ruíz R,Aguado C,Martín-Belmonte A,Moreno-Martínez AE,Luján R

    更新日期：2019-01-09 00:00:00

  • Molecular Investigation on a Triple Negative Breast Cancer Xenograft Model Exposed to Proton Beams.

    abstract：:Specific breast cancer (BC) subtypes are associated with bad prognoses due to the absence of successful treatment plans. The triple-negative breast cancer (TNBC) subtype, with estrogen (ER), progesterone (PR) and human epidermal growth factor-2 (HER2) negative receptor status, is a clinical challenge for oncologists, ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21176337

    authors： Cammarata FP,Forte GI,Broggi G,Bravatà V,Minafra L,Pisciotta P,Calvaruso M,Tringali R,Tomasello B,Torrisi F,Petringa G,Cirrone GAP,Cuttone G,Acquaviva R,Caltabiano R,Russo G

    更新日期：2020-09-01 00:00:00

  • A Peptidomimetic Fluorescent Probe to Detect the Trypsin β2 Subunit of the Human 20S Proteasome.

    abstract：:This work describes the chemical synthesis, combinatorial selection, and enzymatic evaluation of peptidomimetic fluorescent substrates specific for the trypsin-like (β2) subunit of the 20S human proteasome. After deconvolution of a library comprising nearly 6000 compounds composed of peg substituted diaminopropionic a...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21072396

    authors： Wysocka M,Romanowska A,Gruba N,Michalska M,Giełdoń A,Lesner A

    更新日期：2020-03-31 00:00:00

  • Nano-ZnO-Induced Drought Tolerance Is Associated with Melatonin Synthesis and Metabolism in Maize.

    abstract：:The applications of ZnO nanoparticles in agriculture have largely contributed to crop growth regulation, quality enhancement, and induction of stress tolerance, while the underlying mechanisms remain elusive. Herein, the involvement of melatonin synthesis and metabolism in the process of nano-ZnO induced drought toler...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21030782

    authors： Sun L,Song F,Guo J,Zhu X,Liu S,Liu F,Li X

    更新日期：2020-01-25 00:00:00

  • Endocannabinoids as Guardians of Metastasis.

    abstract：:Endocannabinoids including anandamide and 2-arachidonoylglycerol are involved in cancer pathophysiology in several ways, including tumor growth and progression, peritumoral inflammation, nausea and cancer pain. Recently we showed that the endocannabinoid profiles are deranged during cancer to an extent that this manif...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms17020230

    authors： Tegeder I

    更新日期：2016-02-10 00:00:00

  • Can NF-κB Be Considered a Valid Drug Target in Neoplastic Diseases? Our Point of View.

    abstract：:Multidrug resistance (MDR), of the innate and acquired types, is one of major problems in treating tumor diseases with a good chance of success. In this review, we examine the key role of nuclear factor-kappa B (NF-κB) to induce MDR in three tumor models characterized precisely by innate or acquired MDR, in particular...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21093070

    authors： Labbozzetta M,Notarbartolo M,Poma P

    更新日期：2020-04-27 00:00:00

  • Dissemination of Genetic Acquisition/Loss Provides a Variety of Quorum Sensing Regulatory Properties in Pseudoalteromonas.

    abstract：:A bstract: Quorum sensing (QS) enables single-celled bacteria to communicate with chemical signals in order to synchronize group-level bacterial behavior. Pseudoalteromonas are marine bacteria found in versatile environments, of which QS regulation for their habitat adaptation is extremely fragmentary. To distinguish ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms19113636

    authors： Yu Z,Ding Y,Yin J,Yu D,Zhang J,Zhang M,Ding M,Zhong W,Qiu J,Li J

    更新日期：2018-11-18 00:00:00