Abstract：
:Several cellular insults can result in sperm DNA fragmentation either on one or both DNA strands. Oxidative damage, premature interruption of the apoptotic process and defects in DNA compaction during spermatogenesis are the main mechanisms that cause DNA breaks in sperm. The two-tailed Comet assay is the only technique that can differentiate single- (SSBs) from double- (DSBs) strand DNA breaks. Increased levels of the phosphorylated isoform of the H2AX histone are directly correlated with DSBs and proposed as a molecular biomarker of DSBs. We have carried out a narrative review on the etiologies associated with SSBs and DSBs in sperm DNA, their association with reproductive outcomes and the mechanisms involved in their repair. Evidence suggests a stronger negative impact of DSBs on reproductive outcomes (fertilization, implantation, miscarriage, pregnancy, and live birth rates) than SSBs, which can be partially overcome by using intracytoplasmic sperm injection (ICSI). In sperm, SSBs are irreversible, whereas DSBs can be repaired by homologous recombination, non-homologous end joining (NHEJ) and alternative NHEJ pathways. Although few studies have been published, further research is warranted to provide a better understanding of the differential effects of sperm SSBs and DSBs on reproductive outcomes as well as the prognostic relevance of DNA breaks discrimination in clinical practice.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Agarwal A,Barbăroșie C,Ambar R,Finelli Rdoi
10.3390/ijms21113882subject
Has Abstractpub_date
2020-05-29 00:00:00issue
11issn
1422-0067pii
ijms21113882journal_volume
21pub_type
杂志文章,评审abstract：:The spatiotemporal regulation of calcium (Ca2+) storage in late endosomes (LE) and lysosomes (Lys) is increasingly recognized to influence a variety of membrane trafficking events, including endocytosis, exocytosis, and autophagy. Alterations in Ca2+ homeostasis within the LE/Lys compartment are implicated in human di...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19051444
更新日期：2018-05-12 00:00:00
abstract：:Tryptanthrin, a kind of indole quinazoline alkaloid, has been shown to exhibit anti-microbial, anti-inflammation and anti-tumor effects both in vivo and in vitro. However, its biological activity on human chronic myeloid leukemia cell line K562 is not fully understood. In the present study, we investigated the prolife...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12063831
更新日期：2011-01-01 00:00:00
abstract：:Simultaneous imaging of multiple targets (SIMT) in opaque biological tissues is an important goal for molecular imaging in the future. Multi-color fluorescence imaging in deep tissues is a promising technology to reach this goal. In this work, we developed a dual-modality imaging system by combining our recently devel...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18020323
更新日期：2017-02-04 00:00:00
abstract：:Gibberellin (GA) is a key signal molecule inducing differentiation of tracheary elements, fibers, and xylogenesis. However the molecular mechanisms underlying the effect of GA on xylem elongation and secondary wall development in tree species remain to be determined. In this study, Betula platyphylla (birch) seeds wer...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160922960
更新日期：2015-09-23 00:00:00
abstract：:The management of severe acetabular bone defects in revision reconstructive orthopedic surgery is challenging. In this study, cyclic precalcification (CP) treatment was used on both nanotube-surface Ti-mesh and a bone graft substitute for the acetabular defect model, and its effects were assessed in vitro and in vivo....
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18071462
更新日期：2017-07-07 00:00:00
abstract：:The scanning speed of atomic force microscopes continues to advance with some current commercial microscopes achieving on the order of one frame per second and at least one reaching 10 frames per second. Despite the success of these instruments, even higher frame rates are needed with scan ranges larger than are curre...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19041044
更新日期：2018-03-31 00:00:00
abstract：:Endothelial cells (ECs) that line the lumen of blood vessels are important players in blood vessel formation, and EC migration is a key component of the angiogenic process. Thus, identification of genes that are specifically or preferentially expressed in vascular ECs and in-depth understanding of their biological fun...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13044920
更新日期：2012-01-01 00:00:00
abstract：:Due to a typesetting error during layout, several references were incorrectly listed in [...]. ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,已发布勘误
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更新日期：2019-05-29 00:00:00
abstract：:Hemostasis disorder in patients with end-stage renal disease (ESRD) is frequently associated with bleeding diathesis but it may also manifest in thrombotic complications. Analysis of individual coagulation and fibrinolytic factors may shed light on the background of this paradox situation. Here we explored components ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228426
更新日期：2020-11-10 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms131013104
更新日期：2012-10-12 00:00:00
abstract：:Heme oxygenase 1 (HO-1) and carbon monoxide were shown to normalize oxidative stress and inflammatory reactions induced by neuropathic pain in the central nervous system, but their effects in the locus coeruleus (LC) of animals with peripheral inflammation and their interaction with nitric oxide are unknown. In wild-t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20092211
更新日期：2019-05-05 00:00:00
abstract：:Saturated aqueous solutions of various common salts were examined for their effect on aqueous aldol reactions catalysted by a highly active C(2)-symmetric diprolinamide organocatalyst developed in our laboratory. With respect to the aldol reaction between cyclohexanone and 4-nitrobenzaldehyde, deionised water was alwa...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12129083
更新日期：2011-01-01 00:00:00
abstract：:The intestinal epithelium constitutes a dynamic physical barrier segregating the luminal content from the underlying mucosal tissue. Following injury, the epithelial integrity is restored by rapid migration of intestinal epithelial cells (IECs) across the denuded area in a process known as wound healing. Hence, throug...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms15069594
更新日期：2014-05-30 00:00:00
abstract：:Impairment of skeletal muscle function causes disabilities in elderly people. Therefore, in an aged society, prevention and treatment of sarcopenia are important for expanding healthy life expectancy. In addition to aging, adipose tissue disfunction and inflammation also contribute to the pathogenesis of sarcopenia by...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21093182
更新日期：2020-04-30 00:00:00
abstract：:With the exception of a few Mendelian traits, almost all phenotypes (traits) in livestock science are quantitative or complex traits regulated by the expression of many genes. For most of the complex traits, differential expression of genes, rather than genomic variation in the gene coding sequences, is associated wit...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18020472
更新日期：2017-02-22 00:00:00
abstract：:Abnormalities in the melatonin signaling pathway and the involvement of melatonin receptor MT2 have been reported in patients with adolescent idiopathic scoliosis (AIS). Whether these abnormalities were involved in the systemic abnormal skeletal growth in AIS during the peripubertal period remain unknown. In this cros...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150917100
更新日期：2014-09-25 00:00:00
abstract：:Polyamines (PAs) are natural compounds involved in many growth and developmental processes in plants, and, specifically in fruits, play a vital role regulating its development, ripening and senescence processes. Putrescine (PUT), spermine (SPE), and spermidine (SPD) are prominent PAs applied exogenously to extend shel...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18081789
更新日期：2017-08-17 00:00:00
abstract：:Esterification of starch was carried out to expand the usefulness of starch for a myriad of industrial applications. Lipase B from Candida antarctica, immobilized on macroporous acrylic resin (Novozym 435), was used for starch esterification in two reaction systems: micro-solvent system and solvent-free system. The es...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13067226
更新日期：2012-01-01 00:00:00
abstract：:Interstitial telomeric sequences (ITSs) are short stretches of telomeric-like repeats (TTAGGG)n at nonterminal chromosomal sites. We previously demonstrated that, in the genomes of primates and rodents, ITSs were inserted during the repair of DNA double-strand breaks. These conclusions were derived from sequence compa...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21082838
更新日期：2020-04-18 00:00:00
abstract：:Gene knock-in techniques have rapidly evolved in recent years, along with the development and maturation of genome editing technology using programmable nucleases. We recently reported a novel strategy for microhomology-mediated end-joining-dependent integration of donor DNA by using TALEN or CRISPR/Cas9 and optimized...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms161023849
更新日期：2015-10-09 00:00:00
abstract：:To uncover mechanisms and explore novel biomarkers of obesity, type 2 diabetes (T2DM) and nonalcoholic steatohepatitis (NASH)-associated hepatocarcinogenesis, cellular and molecular alterations in the liver, and hepatocellular carcinomas (HCCs) were investigated in NASH model 60-week-old Tsumura, Suzuki, Obese Diabeti...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21207746
更新日期：2020-10-20 00:00:00
abstract：:Two mesenchymal zinc transporters, ZIP7 and ZIP13, play critical roles in dermal development. ZIP7 and ZIP13 are the closest among the conserved mammalian zinc transporters. However, whether their functions are complementary remains a controversial issue. In the present study, we found that the expression of ZIP13, bu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2019-08-13 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2020-06-02 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21103661
更新日期：2020-05-22 00:00:00
abstract：:The anti-stress potential of dietary L-arginine (Arg) was assessed in psychosocially stress-loaded senescence-accelerated (SAMP10) mice. Although this strain of mouse is sensitive to stress, daily administration of Arg at 3 mg/kg significantly suppressed aging-related cognitive decline and behavioral depression at nin...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020508
更新日期：2021-01-06 00:00:00
abstract：:The shortage of donor organs is a major global concern. Organ failure requires the transplantation of functional organs. Donor's organs are preserved for variable periods of warm and cold ischemia time, which requires placing them into a preservation device. Ischemia and reperfusion damage the organs, due to the lack ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20215511
更新日期：2019-11-05 00:00:00
abstract：:We report molecular dynamics simulations of confined liquid crystals using the Gay-Berne-Kihara model. Upon isobaric cooling, the standard sequence of isotropic-nematic-smectic A phase transitions is found. Upon further cooling a reentrant nematic phase occurs. We investigate the temperature dependence of the self-dif...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13067854
更新日期：2012-01-01 00:00:00
abstract：:Sphingosine-1-phosphate (S1P) is a highly potent sphingolipid metabolite, which controls numerous physiological and pathological process via its extracellular and intracellular functions. The breast is mainly composed of epithelial cells (mammary gland) and adipocytes (stroma). Adipocytes play an important role in reg...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21239284
更新日期：2020-12-05 00:00:00
abstract：:Bisphenol A (BPA) is a widespread pollutant threatening the ecosystem and human health. An effective BPA degrader YC-JY1 was isolated and identified as Sphingobium sp. The optimal temperature and pH for the degradation of BPA by strain YC-JY1 were 30 °C and 6.5, respectively. The biodegradation pathway was proposed ba...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21103588
更新日期：2020-05-19 00:00:00
abstract：:Brain-derived neurothrophic factor (BDNF) is a neurotrophin expressed in different tissues and cells, including neurons, endothelial cells, leukocytes, megakaryocytes and platelets. Modifications of BDNF in plasma and/or in serum are associated with neurodegenerative and psychiatric disorders, cardiovascular diseases,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18091987
更新日期：2017-09-15 00:00:00
