Abstract:
:Exosomes are membranous nanovesicles of endocytic origin that carry and transfer regulatory bioactive molecules and mediate intercellular communication between cells and tissues. Although seminal exosomes have been identified in human seminal plasma, their exact composition and possible physiologic function remain unknown. The objective of this study was to perform a comprehensive proteomics analysis of exosomes derived from human seminal plasma. Seminal exosomes were isolated and purified from 12 healthy donors using a 30% sucrose cushion-based exosome-isolation protocol, followed by characterization by western blot, transmission electron microscopy, and nanoparticle tracking analysis before performing extensive liquid chromatography tandem mass spectrometry proteomics analysis. The identified proteins were analyzed by bioinformatics analysis, and seminal exosomes-associated proteins were selectively validated by western blot. A total of 1474 proteins were identified in all seminal exosomes samples, with Gene Ontology analysis demonstrating that these identified seminal exosomes-associated proteins were mostly linked to 'exosomes,' 'cytoplasm,' and 'cytosol.' Bioinformatics analysis indicated that these proteins were mainly involved in biologic processes, including metabolism, energy pathways, protein metabolism, cell growth and maintenance, and transport. Of these identified proteins, PHGDH, LGALS3BP, SEMG1, ACTB, GAPDH, and the exosomal-marker protein ALIX were validated by western blot. This study provided a more comprehensive description of the seminal exosomes proteome and could also be a resource for further screening of biomarkers and comparative proteomics studies, including those associated with male infertility and prostate cancer.
journal_name
Andrologyjournal_title
Andrologyauthors
Yang C,Guo WB,Zhang WS,Bian J,Yang JK,Zhou QZ,Chen MK,Peng W,Qi T,Wang CY,Liu CDdoi
10.1111/andr.12412subject
Has Abstractpub_date
2017-09-01 00:00:00pages
1007-1015issue
5eissn
2047-2919issn
2047-2927journal_volume
5pub_type
杂志文章相关文献
Andrology文献大全abstract:BACKGROUND:Men with congenital unilateral absence of vas deferens were reported to be mainly azoospermic, with both unilateral renal absence and mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) but some have neither. OBJECTIVES:To assess whether in infertile couples the male partners with co...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12749
更新日期:2020-05-01 00:00:00
abstract::In males, age-related decline in free testosterone (T) and dehydroepiandrosterone (DHEA) by 2-3% per year has been reported. Estradiol (E2) and progesterone (P) seem to decrease as well, but to a lesser extent. Lower sex steroid levels in men have been related to physical and mental symptoms. Low birthweight and left-...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12516
更新日期:2018-11-01 00:00:00
abstract::Escherichia coli (E. coli) is a common pathogen in epididymitis, which represents a prevalent entity in male reproductive tract infections (RTI). Although current treatment regimens using antibiotics are satisfactory, development of antimicrobial resistance by the pathogen represents a challenge in the management of R...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12012
更新日期:2015-03-01 00:00:00
abstract::Brain dysfunction in erectile dysfunction (ED) has been identified by multiple neuroimaging studies. A recent MRI study indicated grey matter alterations in ED patients. This study aims to investigate the microstructural changes of cerebral white matter (WM) in psychological ED patients and their possible correlations...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/j.2047-2927.2014.00191.x
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND:Recent evidence suggests that routine semen analysis has limited power to predict male fertility. Several efforts have been made to best discriminate between fertile and subfertile men using several sperm features. So, there is a need to more reliable assays for providing molecular data to evaluate sperm qua...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12801
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Penile cancer represents a rare pathology whose natural history of treatment is poorly understood. OBJECTIVE:To illustrate the dynamic survival profiles in surgically treated patients with squamous cell carcinoma of the penis (SCCP) using the conditional survival (CS) estimates. MATERIALS AND METHODS:Patie...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12856
更新日期:2020-11-01 00:00:00
abstract::Fibroblast growth factors (FGFs) and their receptors (FGFRs) regulate several functions of somatic cells. In a previous work, we reported FGFR expression in human spermatozoa and their involvement in motility. This study aimed to evaluate the presence and localization of fibroblast growth factor 2 (FGF2) in human sper...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12398
更新日期:2017-09-01 00:00:00
abstract::In this study a Bayesian network (BN) has been built for the study of the objective motility of Tinca tinca spermatozoa (spz). Semen from eight 2-year-old sexually mature male tenchs was obtained and motility analyses were performed at 6-17, 23-34 and 40-51 s after activation, using computer-assisted sperm analysis (C...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12071
更新日期:2015-09-01 00:00:00
abstract::Maternal overweight and obesity in pregnancy has been associated with earlier age of menarche in daughters as well as reduced semen quality in sons. We aimed at investigating pubertal development in sons born by mothers with a high body mass index (BMI). The study included 2522 sons of mothers that during pregnancy in...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/j.2047-2927.2013.00171.x
更新日期:2014-03-01 00:00:00
abstract::Spermatogenesis involves the aggregated action of up to 2300 genes, any of which, could, potentially, provide targets for diagnostic tests of male factor infertility. Contrary to the previously proposed common variant hypothesis for common diseases such as male infertility, genome-wide association studies and targeted...
journal_title:Andrology
pub_type: 杂志文章,评审
doi:10.1111/j.2047-2927.2014.00200.x
更新日期:2014-05-01 00:00:00
abstract::Germ cell and Sertoli cell proliferation and maturation in human testes occur in three main waves, during the late fetal and early neonatal period and at early puberty. They are triggered by periods of increased activity of the hypothalamic-pituitary-gonadal (HPG) axis. In hypogonadotropic hypogonadism (HH), these pro...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12128
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:Testicular germ cell tumour (TGCT) is a malignancy with a high heritable component. The inherited risk is polygenic, and around 50 susceptibility genes are identified. The functional role of the gene products for TGCT development is not well understood. The focus of this review is functional studies of geneti...
journal_title:Andrology
pub_type: 杂志文章,评审
doi:10.1111/andr.12663
更新日期:2019-07-01 00:00:00
abstract::To add new arguments concerning the origin of the sperm-head vacuoles observed under high magnification with interference contrast microscopy, we carried out in two patients with total globozoospermia confirmed using transmission electron microscopy (TEM), a detailed sperm morphometric analysis with high magnification...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/j.2047-2927.2012.00011.x
更新日期:2013-01-01 00:00:00
abstract::Existing literature suggests evidence that protamine deficiency is related to DNA damage and male fertility. In this meta-analysis, we analyzed the relationship between the ratio of protamine-1 and protamine-2 with male fertility and the association of protamine deficiency with sperm DNA damage. Quality of available c...
journal_title:Andrology
pub_type: 杂志文章,meta分析,评审
doi:10.1111/andr.12216
更新日期:2016-09-01 00:00:00
abstract::In cryptorchid boys, failures in germ cell development have been clearly established. Some studies reported some abnormalities in Sertoli cells morphology but the results regarding their endocrine secretion remain controversial. To compare testicular hormone levels in young boys with and without cryptorchidism, we per...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12373
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:We previously demonstrated how mouse spermatozoa can be efficiently stored for two years in a -80°C freezer, maintaining their ability to fertilize mouse eggs. OBJECTIVES:The main objective here was to evaluate the effects of five years at -80°C compared to liquid nitrogen storage (LN2 , control condition) ...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12971
更新日期:2021-01-11 00:00:00
abstract::Erectile Dysfunction is considered a multifactorial disease, where organic and psychological aspects are often interconnected. In a randomized controlled pilot study, we compared the efficacy of combined vardenafil orodispersible tablet (VARD) and cognitive-behavioral sex therapy (CBST) vs. VARD alone in improving sex...
journal_title:Andrology
pub_type: 杂志文章,随机对照试验
doi:10.1111/andr.12079
更新日期:2015-09-01 00:00:00
abstract::Tributyltin (TBT), a pesticide used in antifouling paints, is toxic for aquatic invertebrates. In vertebrates, TBT may act in obesogen- inducing adipogenetic gene transcription for adipocyte differentiation. In a previous study, we demonstrated that acute administration of TBT induces c-fos expression in the arcuate n...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12222
更新日期:2016-07-01 00:00:00
abstract::Deletions on the long arm of the Y chromosome are a well-known cause of male infertility and it is generally accepted that deletions involving the AZFb region are not compatible with sperm production. Here, we report on two patients for whom basic diagnostic tests showed a deletion of the AZFb region. Unexpectedly, bo...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12350
更新日期:2017-07-01 00:00:00
abstract::Pubertal gynecomastia is a common condition observed in up to 65% of adolescent males. It is usually idiopathic and tends to regress within 1-2 years. In this descriptive cross-sectional study, we investigated 25 adolescent males with prominent (>B3) and/or persistent (>2 years) pubertal gynecomastia (P/PPG) to determ...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12145
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:The association between alcohol intake and male reproductive function is still controversial. In the frame of a prospective cohort study, designed to investigate the relation between life style and fertility, we performed a cross-sectional analysis of semen quality. METHODS:Men of subfertile couples, referr...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12521
更新日期:2018-09-01 00:00:00
abstract::A recent study has demonstrated that porcine spermatozoa recognize with high affinity carbohydrate structures containing Lewis X motifs. Sperm adhesion to Lewis X is proposed to mediate sperm binding to the oviduct epithelium to form a reservoir. The objective of this study was to identify Lewis X-binding proteins fro...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12340
更新日期:2017-05-01 00:00:00
abstract::The main aim of this work was to evaluate how supplementing freezing media with reduced glutathione (GSH) affected the 'in vivo' fertilizing ability of boar semen subjected to cryopreservation procedures. With this purpose, 12 ejaculates coming from 12 boars were cryopreserved in the presence or absence of 2 mm GSH, w...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/j.2047-2927.2013.00144.x
更新日期:2014-01-01 00:00:00
abstract::Spermiogenesis is the final phase of spermatogenesis. During this process, haploid round spermatids differentiate into spermatozoa, with dramatic morphological changes, including elongation and condensation of the nuclei, and formation of the flagella. Meig1 is one of many genes involved in the regulation of this proc...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/j.2047-2927.2013.00069.x
更新日期:2013-05-01 00:00:00
abstract::The double sex and mab-3-related transcription factor 1 (DMRT1) gene has long been linked to sex-determining pathways across vertebrates and is known to play an essential role in gonadal development and maintenance of spermatogenesis in mice. In humans, the genomic region harboring the DMRT gene cluster has been impli...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12063
更新日期:2015-09-01 00:00:00
abstract::Increasing evidence disclosed the existence of a novel multi-organ endocrine pathway, involving bone, pancreas and testis, of high penetrance in energy metabolism and male fertility. The main mediator of this axis is undercarboxylated osteocalcin (ucOC), a bone-derived protein-exerting systemic effects on tissues expr...
journal_title:Andrology
pub_type: 杂志文章,评审
doi:10.1111/andr.12359
更新日期:2017-07-01 00:00:00
abstract::Semen quality is suggested to be a universal biomarker for future health. Previous studies have mostly been registry based excluding the possibility to address the importance of lifestyle, fertility status, health and socio-economic status. We aimed to investigate whether the association between semen quality and subs...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12477
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:The transcription factor DMRTB1 plays a pivotal role in coordinating the transition between mitosis and meiosis in murine germ cells. No reliable data are available for human testis. OBJECTIVES:The present study aims to examine the testicular expression pattern of DMRTB1 in men showing normal and impaired s...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12617
更新日期:2019-07-01 00:00:00
abstract::The second messenger cyclic adenosine monophosphate (cAMP) has a central role in sperm physiology. Extracellular cAMP can be sequentially degraded into 5'AMP and adenosine by ecto-phosphodiesterases (ecto-PDE) and ecto-nucleotidases, a phenomenon called extracellular cAMP-adenosine pathway. As cAMP-adenosine pathway i...
journal_title:Andrology
pub_type: 杂志文章
doi:10.1111/andr.12267
更新日期:2016-11-01 00:00:00
abstract::Urethral stricture disease, pelvic fracture urethral injury (PFUI), and their various treatment options are associated with erectile dysfunction (ED). The etiology of urethral stricture disease is multifactorial and includes trauma, inflammatory, and iatrogenic causes. Posterior urethral injuries are commonly associat...
journal_title:Andrology
pub_type: 杂志文章,评审
doi:10.1111/andr.12015
更新日期:2015-05-01 00:00:00