Supplementing cryopreservation media with reduced glutathione increases fertility and prolificacy of sows inseminated with frozen-thawed boar semen.

Abstract:

:The main aim of this work was to evaluate how supplementing freezing media with reduced glutathione (GSH) affected the 'in vivo' fertilizing ability of boar semen subjected to cryopreservation procedures. With this purpose, 12 ejaculates coming from 12 boars were cryopreserved in the presence or absence of 2 mm GSH, whereas the same number of extended ejaculates coming from the same boars was used as negative/farm controls. Eight different sperm parameters (levels of free-cysteine residues in sperm nucleoproteins, DNA fragmentation, sperm viability, acrosome-membrane integrity, intracellular peroxide and superoxide levels, and total and progressive sperm motility) were evaluated before freezing and after 30 and 240 min of thawing. In addition, a total of 180 multiparous sows were used in the field fertility trials, the females being randomly divided into three groups and inseminated with extended, frozen-thawed control or frozen-thawed semen supplemented with 2 mm GSH. The presence of GSH in the freezing media significantly (p < 0.05) increased farrowing rates and the number of total born piglets and alive born piglets, and partially counteracted the cryopreservation-induced damages inflicted on frozen-thawed spermatozoa. We can thus conclude that supplementing freezing media with 2 mm GSH greatly improves boar sperm cryopreservation technology, as it significantly improves the fertilizing ability of frozen-thawed spermatozoa.

journal_name

Andrology

journal_title

Andrology

authors

Estrada E,Rodríguez-Gil JE,Rocha LG,Balasch S,Bonet S,Yeste M

doi

10.1111/j.2047-2927.2013.00144.x

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

88-99

issue

1

eissn

2047-2919

issn

2047-2927

journal_volume

2

pub_type

杂志文章
  • Calibration of redox potential in sperm wash media and evaluation of oxidation-reduction potential values in various assisted reproductive technology culture media using MiOXSYS system.

    abstract::Oxidation-reduction potential describes the balance between the oxidants and antioxidants in fluids including semen. Various artificial culture media are used in andrology and IVF laboratories for sperm preparation and to support the development of fertilized oocytes under in vitro conditions. The composition and cond...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12461

    authors: Panner Selvam MK,Henkel R,Sharma R,Agarwal A

    更新日期:2018-03-01 00:00:00

  • Light and electron microscopic study of mature spermatozoa from White Pekin duck (Anas platyrhynchos): an ultrastructural and molecular analysis.

    abstract::The morphology, ultrastructure, and functions of mature avian spermatozoa have been of immense commercial and scientific interest for several reasons. This is mainly important in case of birds in poultry production, conservation, and in the use of sperm morphometry as phylogenetic evidence. Avian spermatozoa share com...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12130

    authors: Majhi RK,Kumar A,Yadav M,Kumar P,Maity A,Giri SC,Goswami C

    更新日期:2016-03-01 00:00:00

  • Differential gene expression profiles of human efferent ducts and proximal epididymis.

    abstract:BACKGROUND:Gene expression patterns along the epididymis are established by specific transcription factor networks that coordinate region-specific functions. In rodents, the epididymis can be divided in up to 19 segments. Based on gross anatomy, the human epididymis is divided into caput, corpus, and cauda segments tog...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12745

    authors: Légaré C,Sullivan R

    更新日期:2020-05-01 00:00:00

  • Osteocalcin is associated with testosterone in the general population and selected patients with bone disorders.

    abstract::Research in the last decade has revealed that bone is not only a target tissue for numerous circulating hormones but functions as an endocrine organ itself. As a recent study demonstrated a stimulatory effect of the osteoblast-derived hormone osteocalcin (OCN) on testosterone production in mice, we investigated whethe...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/j.2047-2927.2012.00044.x

    authors: Hannemann A,Breer S,Wallaschofski H,Nauck M,Baumeister SE,Barvencik F,Amling M,Schinke T,Haring R,Keller J

    更新日期:2013-05-01 00:00:00

  • Meiotic gene activation in somatic and germ cell tumours.

    abstract:BACKGROUND:Germ cell tumours are uniquely associated with the gametogenic tissues of males and females. A feature of these cancers is that they can express genes that are normally tightly restricted to meiotic cells. This aberrant gene expression has been used as an indicator that these cancer cells are attempting a pr...

    journal_title:Andrology

    pub_type: 杂志文章,评审

    doi:10.1111/andr.12628

    authors: Feichtinger J,McFarlane RJ

    更新日期:2019-07-01 00:00:00

  • Rare double sex and mab-3-related transcription factor 1 regulatory variants in severe spermatogenic failure.

    abstract::The double sex and mab-3-related transcription factor 1 (DMRT1) gene has long been linked to sex-determining pathways across vertebrates and is known to play an essential role in gonadal development and maintenance of spermatogenesis in mice. In humans, the genomic region harboring the DMRT gene cluster has been impli...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12063

    authors: Lima AC,Carvalho F,Gonçalves J,Fernandes S,Marques PI,Sousa M,Barros A,Seixas S,Amorim A,Conrad DF,Lopes AM

    更新日期:2015-09-01 00:00:00

  • European academy of andrology guidelines on Klinefelter Syndrome Endorsing Organization: European Society of Endocrinology.

    abstract:BACKGROUND:Knowledge about Klinefelter syndrome (KS) has increased substantially since its first description almost 80 years ago. A variety of treatment options concerning the spectrum of symptoms associated with KS exists, also regarding aspects beyond testicular dysfunction. Nevertheless, the diagnostic rate is still...

    journal_title:Andrology

    pub_type: 杂志文章,评审

    doi:10.1111/andr.12909

    authors: Zitzmann M,Aksglaede L,Corona G,Isidori AM,Juul A,T'Sjoen G,Kliesch S,D'Hauwers K,Toppari J,Słowikowska-Hilczer J,Tüttelmann F,Ferlin A

    更新日期:2021-01-01 00:00:00

  • Clinical analysis of the characterization of magnetic resonance imaging in 102 cases of refractory haematospermia.

    abstract::To analyze the pathogenesis of persistent and refractory haematospermia and to evaluate the aetiological diagnostic value of magnetic resonance imaging (MRI) for this type of haematospermia. Clinical data from 102 patients with persistent and refractory haematospermia was retrospectively analysed. Data collected inclu...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/j.2047-2927.2013.00132.x

    authors: Li BJ,Zhang C,Li K,Zhang J,Zhang Y,Sun ZY,Kang HY,Zhou B,Jin FS,Zhang KQ,Li YF

    更新日期:2013-11-01 00:00:00

  • Naringenin attenuates highly active antiretroviral therapy-induced sperm DNA fragmentations and testicular toxicity in Sprague-Dawley rats.

    abstract::Highly active antiretroviral therapy has evolved over the years, leading to a boost in the quality of life in people living with HIV and AIDS. However, growing evidence has shown that highly active antiretroviral therapy has deleterious effects on the testes and the overall reproductive capacity. Therefore, this study...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12439

    authors: Adana MY,Akang EN,Peter AI,Jegede AI,Naidu ECS,Tiloke C,Chuturgoon AA,Azu OO

    更新日期:2018-01-01 00:00:00

  • Immunolocalization of DMRTB1 in human testis with normal and impaired spermatogenesis.

    abstract:BACKGROUND:The transcription factor DMRTB1 plays a pivotal role in coordinating the transition between mitosis and meiosis in murine germ cells. No reliable data are available for human testis. OBJECTIVES:The present study aims to examine the testicular expression pattern of DMRTB1 in men showing normal and impaired s...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12617

    authors: Hilbold E,Bergmann M,Fietz D,Kliesch S,Weidner W,Langeheine M,Rode K,Brehm R

    更新日期:2019-07-01 00:00:00

  • Long-term follow-up of ICSI-conceived offspring compared with spontaneously conceived offspring: a systematic review of health outcomes beyond the neonatal period.

    abstract:BACKGROUND:A significant increase in the use of intracytoplasmic sperm injection (ICSI) since its introduction in 1992 has been observed worldwide, including beyond its original intended use for severe male factor infertility. Concerns regarding ICSI include the effects of poor quality spermatozoa on offspring health a...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12526

    authors: Catford SR,McLachlan RI,O'Bryan MK,Halliday JL

    更新日期:2018-09-01 00:00:00

  • A data-driven search for semen-related phenotypes in conception delay.

    abstract::Sperm count, morphology, and motility have been reported to be predictive of pregnancy, although with equivocal basis prompting some authors to question the prognostic value of semen analysis. To assess the utility of including semen quality data in predicting conception delay or requiring >6 cycles to become pregnant...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12288

    authors: Patel CJ,Sundaram R,Buck Louis GM

    更新日期:2017-01-01 00:00:00

  • Involvement of mitochondrial dysfunction in the adverse effect exerted by seminal plasma from men with spinal cord injury on sperm motility.

    abstract::The aetiology of severe asthenozoospermia in men with spinal cord injury includes an adverse impact of seminal plasma (SP) on sperm motility. In this study we investigated the effect exerted by SP from men with SCI on donor sperm mitochondrial activity and its reflection on motility. Donor spermatozoa were exposed (1 ...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/j.2047-2927.2013.00077.x

    authors: Barbonetti A,Vassallo MR,Di Rosa A,Leombruni Y,Felzani G,Gandini L,Lenzi A,Necozione S,Francavilla S,Francavilla F

    更新日期:2013-05-01 00:00:00

  • Are AZFb deletions always incompatible with sperm production?

    abstract::Deletions on the long arm of the Y chromosome are a well-known cause of male infertility and it is generally accepted that deletions involving the AZFb region are not compatible with sperm production. Here, we report on two patients for whom basic diagnostic tests showed a deletion of the AZFb region. Unexpectedly, bo...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12350

    authors: Stouffs K,Vloeberghs V,Gheldof A,Tournaye H,Seneca S

    更新日期:2017-07-01 00:00:00

  • Levels of l-arginine and l-citrulline in patients with erectile dysfunction of different etiology.

    abstract::Nitric oxide is a physiologic signal essential to penile erection. l-citrulline (l-Cit) is converted into l-arginine (l-Arg), the precursor from which nitric oxide is generated. The level of l-Arg and l-Cit in the field of male sexual function remains relatively underexplored. The aim of the study was to evaluate the ...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12293

    authors: Barassi A,Corsi Romanelli MM,Pezzilli R,Damele CA,Vaccalluzzo L,Goi G,Papini N,Colpi GM,Massaccesi L,Melzi d'Eril GV

    更新日期:2017-03-01 00:00:00

  • Revisiting the impact of ejaculatory abstinence on semen quality and intracytoplasmic sperm injection outcomes.

    abstract:BACKGROUND:Regulatory bodies recommend inconsistent ejaculatory abstinence lengths before semen analysis. The literature exploring the effect of ejaculatory abstinence length on the outcomes of intracytoplasmic sperm injection is scarce. OBJECTIVE:To study the influence of ejaculatory abstinence length on semen qualit...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12572

    authors: Borges E Jr,Braga DPAF,Zanetti BF,Iaconelli A Jr,Setti AS

    更新日期:2019-03-01 00:00:00

  • Osteocalcin, a bone-derived hormone with important andrological implications.

    abstract::Increasing evidence disclosed the existence of a novel multi-organ endocrine pathway, involving bone, pancreas and testis, of high penetrance in energy metabolism and male fertility. The main mediator of this axis is undercarboxylated osteocalcin (ucOC), a bone-derived protein-exerting systemic effects on tissues expr...

    journal_title:Andrology

    pub_type: 杂志文章,评审

    doi:10.1111/andr.12359

    authors: De Toni L,Di Nisio A,Rocca MS,De Rocco Ponce M,Ferlin A,Foresta C

    更新日期:2017-07-01 00:00:00

  • Impact of time from diagnosis to treatment on erectile function outcomes after radical prostatectomy.

    abstract:BACKGROUND:Concerns exist about the effect of delaying treatment for prostate cancer (PCa) regarding both oncological and functional outcomes after radical prostatectomy (RP). OBJECTIVE:To assess the impact of time from diagnosis to RP on post-operative erectile function (EF) outcomes. MATERIALS AND METHODS:We analyz...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12699

    authors: Schifano N,Capogrosso P,Pozzi E,Ventimiglia E,Cazzaniga W,Matloob R,Gandaglia G,Dehò F,Briganti A,Montorsi F,Salonia A

    更新日期:2020-03-01 00:00:00

  • Semen quality associated with subsequent hospitalizations - Can the effect be explained by socio-economic status and lifestyle factors?

    abstract::Semen quality is suggested to be a universal biomarker for future health. Previous studies have mostly been registry based excluding the possibility to address the importance of lifestyle, fertility status, health and socio-economic status. We aimed to investigate whether the association between semen quality and subs...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12477

    authors: Latif T,Lindahl-Jacobsen R,Mehlsen J,Eisenberg ML,Holmboe SA,Pors K,Brinth L,Skouby SO,Jørgensen N,Jensen TK

    更新日期:2018-05-01 00:00:00

  • Combined effects of arsenic exposure and diabetes on male reproductive functions.

    abstract:BACKGROUND:It is known that exposure to either arsenic or hyperglycemia can induce male reproductive damages. However, their combined effects on male reproductive organs are still unclear. Therefore, the present study investigated morphological and functional parameters of the testis, epididymis, and spermatozoa in dia...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12613

    authors: Souza ACF,Bastos DSS,Sertorio MN,Santos FC,Ervilha LOG,de Oliveira LL,Machado-Neves M

    更新日期:2019-09-01 00:00:00

  • Increased expression of TRPC4 channels associated with erectile dysfunction in diabetes.

    abstract::In recent reports, an association between altered TRPC channel function and the development of various diabetic complications has drawn the attention of many investigators. The aim of this study was to investigate the expression of TRPC4 channels of corpus smooth muscle (CSM) cells in diabetes, and to evaluate the ass...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/j.2047-2927.2014.00214.x

    authors: Sung HH,Choo SH,Ko M,Kang SJ,Chae MR,Kam SC,Han DH,So I,Lee SW

    更新日期:2014-07-01 00:00:00

  • Testosterone replacement in transgenic sickle cell mice controls priapic activity and upregulates PDE5 expression and eNOS activity in the penis.

    abstract::Sickle cell disease (SCD)-associated priapism is characterized by decreased nitric oxide (NO) signaling and downregulated phosphodiesterase (PDE)5 protein expression and activity in the penis. Priapism is also associated with testosterone deficiency, but molecular mechanisms underlying testosterone effects in the peni...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12442

    authors: Musicki B,Karakus S,Akakpo W,Silva FH,Liu J,Chen H,Zirkin BR,Burnett AL

    更新日期:2018-01-01 00:00:00

  • Lactadherin is a candidate oviduct Lewis X trisaccharide receptor on porcine spermatozoa.

    abstract::A recent study has demonstrated that porcine spermatozoa recognize with high affinity carbohydrate structures containing Lewis X motifs. Sperm adhesion to Lewis X is proposed to mediate sperm binding to the oviduct epithelium to form a reservoir. The objective of this study was to identify Lewis X-binding proteins fro...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12340

    authors: Silva E,Frost D,Li L,Bovin N,Miller DJ

    更新日期:2017-05-01 00:00:00

  • The role of resistin on metabolic syndrome-induced erectile dysfunction and the possible therapeutic effect of Boldine.

    abstract:BACKGROUND:Resistin is known as a potential mediator of obesity-associated insulin resistance. The high resistin level disrupts nitric oxide (NO)-mediated relaxation which is also important in erectile function. An antioxidant alkaloid, Boldine, is known as anti-diabetic and protects endothelial functions. OBJECTIVES:...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12853

    authors: Kara E,Kahraman E,Dayar E,Yetik Anacak G,Demir O,Gidener S,Atabey N,Durmus N

    更新日期:2020-11-01 00:00:00

  • Biology of childhood germ cell tumours, focussing on the significance of microRNAs.

    abstract::Genomic and protein-coding transcriptomic data have suggested that germ cell tumours (GCTs) of childhood are biologically distinct from those of adulthood. Global messenger RNA profiles segregate malignant GCTs primarily by histology, but then also by age, with numerous transcripts showing age-related differential exp...

    journal_title:Andrology

    pub_type: 杂志文章,评审

    doi:10.1111/andr.277

    authors: Murray MJ,Nicholson JC,Coleman N

    更新日期:2015-01-01 00:00:00

  • The association between sperm head elongation and semen quality.

    abstract:BACKGROUND:Previous studies suggested that sperm head shape may serve as an effective indicator of semen quality. However, there lacks research with large sample and quantitative measurement. OBJECTIVES:The objective of this retrospective study was to explore the association between sperm head elongation (Width/Length...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12619

    authors: Chen F,Gao L,Zhou H,Guo L,Chen Q,Gan Y,Sun X,Li Q,Wang K

    更新日期:2019-11-01 00:00:00

  • White matter microstructural changes in psychogenic erectile dysfunction patients.

    abstract::Brain dysfunction in erectile dysfunction (ED) has been identified by multiple neuroimaging studies. A recent MRI study indicated grey matter alterations in ED patients. This study aims to investigate the microstructural changes of cerebral white matter (WM) in psychological ED patients and their possible correlations...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/j.2047-2927.2014.00191.x

    authors: Zhang P,Liu J,Li G,Pan J,Li Z,Liu Q,Qin W,Dong M,Sun J,Huang X,Wu T,Chang D

    更新日期:2014-05-01 00:00:00

  • Therapeutic advances in the treatment of Peyronie's disease.

    abstract::Peyronie's disease (PD) is an under-diagnosed condition with prevalence in the male population as high as 9%. It is a localized connective tissue disorder of the penis characterized by scarring of the tunica albuginea. Its pathophysiology, however, remains incompletely elucidated. For the management of the acute phase...

    journal_title:Andrology

    pub_type: 杂志文章,评审

    doi:10.1111/andr.12058

    authors: Yafi FA,Pinsky MR,Sangkum P,Hellstrom WJ

    更新日期:2015-07-01 00:00:00

  • The insertion/deletion (I/D) polymorphism in the angiotensin-converting enzyme gene and erectile dysfunction risk: a meta-analysis.

    abstract::Erectile dysfunction (ED) is increasingly recognized as a public health problem. Several studies have reported the influence of the insertion/deletion (I/D) polymorphism in the Angiotensin-converting enzyme (ACE) gene on ED susceptibility, but the results remain controversial. To derive a more precise estimation of th...

    journal_title:Andrology

    pub_type: 杂志文章,meta分析

    doi:10.1111/j.2047-2927.2012.00029.x

    authors: Zhang T,Li WL,He XF,Wu ZY,Liu LH,He SH,Wei AY

    更新日期:2013-03-01 00:00:00

  • Malaysian propolis and metformin mitigate subfertility in streptozotocin-induced diabetic male rats by targeting steroidogenesis, testicular lactate transport, spermatogenesis and mating behaviour.

    abstract:BACKGROUND:Diabetes mellitus is one of the risk factors for male subfertility/infertility. Malaysian propolis is reported to decrease hyperglycaemia in diabetic state. OBJECTIVES:The present study investigated the protective effect of Malaysian propolis on diabetes-induced subfertility/infertility. Additionally, its c...

    journal_title:Andrology

    pub_type: 杂志文章

    doi:10.1111/andr.12739

    authors: Nna VU,Bakar ABA,Ahmad A,Umar UZ,Suleiman JB,Zakaria Z,Othman ZA,Mohamed M

    更新日期:2020-05-01 00:00:00