当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Ca2+-induced stabilization of the nucleoside 2'- deoxyribosyltransferase from Lactobacillus hilgardii ZJS01: Characteristics and application in nucleosides synthesis.

Ca2+-induced stabilization of the nucleoside 2'- deoxyribosyltransferase from Lactobacillus hilgardii ZJS01: Characteristics and application in nucleosides synthesis.

Abstract：

:A novel nucleoside 2'-deoxyribosyltransferase (LhNDT) from Lactobacillus hilgardii ZJS01 was cloned and expressed in Escherichia coli BL21 (DE3). The amino acid sequence of LhNDT displayed the highest identity of 26% to other reported NDTs. LhNDT was a monomer as revealed by gel filtration chromatography with or without addition of Ca2+ in elution buffer. This enzyme showed a high activity and stability over a broad pH range and was sensitive to temperature. With the addition of 15mM Ca2+, the thermostability of LhNDT was greatly promoted by 63 times and 46 times at 30°C and 35°C, respectively. With the use of 2'-deoxyuridine as sugar donor at an economic molar ratio of 1:1, 5-aza-2'-deoxycytidine (46%) and 5-fluoro-2'- deoxycytidine (58%) were efficiently synthesized from 5-azacytosine and 5-fluorocytosine, respectively, by LhNDT for 2h.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Zhang J,Zhou Y,Liu K,Chu J,Zhang Y,He B

doi

10.1016/j.ijbiomac.2017.08.104

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

963-968

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(17)31741-5

journal_volume

106

pub_type

杂志文章

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