Abstract:
Purpose:Ocular lens fiber cell elongation, differentiation, and compaction are associated with extensive reorganization of cell adhesive interactions and cytoskeleton; however, our knowledge of proteins critical to these events is still evolving. This study characterizes the distribution pattern of neuronal-specific α-catenin (αN-catenin) and its interaction with the N-cadherin-associated adherens junctions (AJs) and their stability in the mouse lens fibers. Methods:Expression and distribution of αN-catenin in developing mouse and adult human lenses was determined by RT-PCR, immunoblot, and immunofluorescence analyses. Characterization of αN-catenin and N-cadherin interacting proteins and colocalization analyses were performed using immunoprecipitation, mass spectrometry, and confocal imaging. Effects of periaxin deficiency on the stability of lens fiber cell AJs were evaluated using perixin-null mice. Results:αN-catenin exhibits discrete distribution to lens fibers in both mouse and human lenses, undergoing a robust up-regulation during fiber cell differentiation and maturation. Epithelial-specific α-catenin (αE-catenin), in contrast, distributes primarily to the lens epithelium. αN-catenin and N-cadherin reciprocally coimmunoprecipitate and colocalize along with β-catenin, actin, spectrin, vinculin, Armadillo repeat protein deleted in velo-cardio-facial syndrome homolog, periaxin, and ankyrin-B in lens fibers. Fiber cells from periaxin-null mouse lenses revealed disrupted N-cadherin/αN-catenin-based AJs. Conclusions:These results suggest that the discrete shift in α-catenin expression from αE-catenin to αN-catenin subtype that occurs during lens epithelial cell differentiation may play a key role in fiber cell cytoarchitecture by regulating the assembly and stability of N-cadherin-based AJs. This study also provides evidence for the importance of the fiber cell-specific cytoskeletal interacting periaxin, in the stability of N-cadherin/αN-catenin-based AJs in lens fibers.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Maddala R,Rao PVdoi
10.1167/iovs.17-21539subject
Has Abstractpub_date
2017-07-01 00:00:00pages
3445-3455issue
9eissn
0146-0404issn
1552-5783pii
2643582journal_volume
58pub_type
杂志文章abstract:PURPOSE:To compare structural properties from spectral-domain optical coherence tomography (SDOCT) and psychophysical measures from a subset of patients enrolled in a larger multicenter natural history study of X-linked retinoschisis (XLRS). METHODS:A subset of males (n = 24) participating in a larger natural history ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,多中心研究
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abstract:PURPOSE:The epidermal growth factor receptor (EGFR) appears in astrocytes after neural injury. The authors' laboratory has reported the presence of EGFR in glaucomatous optic nerves. The activation of EGFR is often associated with induction of cyclooxygenase (COX)-2. In this study, the induction of COX-2 pathway in rat...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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journal_title:Investigative ophthalmology & visual science
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doi:
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2003-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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更新日期:2015-05-01 00:00:00
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doi:10.1167/iovs.06-0836
更新日期:2007-06-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.09-4926
更新日期:2010-12-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2016-11-01 00:00:00
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doi:10.1167/iovs.03-1304
更新日期:2004-09-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2013-03-13 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.16-20593
更新日期:2017-01-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2004-06-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2016-11-01 00:00:00
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更新日期:2007-08-01 00:00:00
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更新日期:2018-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2017-04-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
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更新日期:2017-06-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2003-04-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2008-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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更新日期:2014-11-25 00:00:00