Abstract:
:Though patients with EGFR mutations are initially responsive to EGFR-tyrosine kinase inhibitors (TKIs), most tumors ultimately acquire resistance to EGFR-TKIs. The most frequently reported mechanism is EGFR T790M mutation. In this study, using a droplet digital PCR (ddPCR) system, we assessed optimal conditions for a mutation detection assay for EGFR T790M obtained from circulating cell-free DNA (cfDNA) in plasma. The advantages of locked nucleic acids (LNA) probe, short amplicon size, and blocking oligo using peptide nucleic acids (PNA) were assessed using control DNAs from cell lines to improve the sensitivity of mutation detection. T790M alleles were then analyzed using ddPCR in 59 plasma samples from 24 NSCLC patients with EGFR mutations, and compared to the T790M status which were determined thorough re-biopsies. The assessment of the optimal assay method revealed that the assay using the short amplicon can efficiently detect more fragmented-DNA. The LNA probe and PNA clamp contributed better separation between positive and negative droplets. This PNA-LNA-ddPCR clamp method can detect mutant alleles in the sample with a mutant allele content of 0.01%. In clinical plasma samples, T790M alleles were detected via ddPCR with a sensitivity of 42.8% and specificity of 97.3%. We established a highly-sensitive detection assay for the T790M allele using the PNA-LNA-ddPCR clamp method. ddPCR is a promising method for detecting non-invasive T790M mutation.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Suzawa K,Yamamoto H,Ohashi K,Hashida S,Tomida S,Kubo T,Maki Y,Soh J,Tsukuda K,Kiura K,Miyoshi S,Toyooka Sdoi
10.3892/or.2017.5567subject
Has Abstractpub_date
2017-05-01 00:00:00pages
3100-3106issue
5eissn
1021-335Xissn
1791-2431journal_volume
37pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::The occurrence of primary extraskeletal Ewing's sarcoma (EES) of the spinal epidural space has been rarely reported in the literature. The clinical, radiologic and pathologic features of a case of EES occurring in the thoracic epidural space are presented. A 37-year-old woman presented with a one-year history of back ...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2011.1326
更新日期:2011-09-01 00:00:00
abstract::We investigated the effects of quercetin on 7,12-dimethylbenz(a)anthracene (DMBA)-induced oxidative stress and the expression of CYP1A1 and CYP1B1 in mice. Quercetin was administered orally to mice at 100 or 250 mg/kg BW for 18 days, after which DMBA (34 mg/kg BW) was administered intragastrically twice. Quercetin sho...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1753
更新日期:2012-07-01 00:00:00
abstract::The aim of this study was to assess the ability of a new magnetic resonance (MR) angiography technique that does not use contrast enhancement medium to depict the vascularity of musculoskeletal neoplasms, and evaluate its clinical utility. We performed 3D fresh blood imaging (FBI) MR angiography in 57 patients with bo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-03-01 00:00:00
abstract::We aimed to investigate the relationship between miRNA-146a and latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) in nasopharyngeal carcinoma (NPC). The expression levels of LMP1 in 40 cases of NPC, 28 cases of chronic nasopharyngitis and NPC cell lines CNE1 and CNE1-GL (...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1933
更新日期:2012-10-01 00:00:00
abstract::Cancer immunotherapy is a promising new approach to cancer treatment. It has been demonstrated that a high number of tumor-specific cytotoxic T cells (CTLs) is associated with increased disease-specific survival in lung cancer patients. Identification of superior CTL epitopes from tumor antigens is essential for the d...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3198
更新日期:2014-07-01 00:00:00
abstract::Telomere-associated proteins function as survival factors in telomere maintenance, which are major contributors to radiosensitivity in human cancers. The aim of this study was to investigate the association of telomere-associated gene expression and radiation resistance in human larynx squamous carcinoma. The changes ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000381
更新日期:2009-06-01 00:00:00
abstract::The main objective was to analyze the influence of social factors on the interval from diagnosis to treatment in a cohort of 217 patients with digestive tract cancer. From the clinical charts data were obtained for: date of the beginning of illness, dates of medical attendance, type and date of diagnostic tests, chara...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract::The phosphatase and tensin homolog (PTEN) gene, an important tumor-suppressor gene, has been demonstrated to have the potential for inhibiting proliferation, migration and invasion in various types of cancer cells. The aim of the present study was to investigate the effect of PTEN expression on osteosarcoma (OS) cells...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3362
更新日期:2014-10-01 00:00:00
abstract::Clear cell renal cell carcinoma (ccRCC) is the main subtype of malignant kidney cancer. Long non‑coding RNA (lncRNA) serves a key role in predicting survival in patients with cancer. The present study aimed to develop an lncRNA‑related signature of prognostic values for patients with ccRCC. RNA sequencing data of 454 ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6540
更新日期:2018-09-01 00:00:00
abstract::DNA intrastrand cross-linking agents such as oxaliplatin induce DNA double-strand breaks (DSBs) during DNA repair and replication. In the present study, we hypothesized that DNA intrastrand cross-linking agents may significantly benefit colorectal cancer patients with deficiencies in DSB repair. Seventy-eight patients...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4488
更新日期:2016-03-01 00:00:00
abstract::Cancer represents a significant challenge for humankind, as early diagnosis and treatment are difficult to achieve. To systemically investigate the effect of sex, body mass index (BMI) and age on cancer incidence and prognosis, the data from 14,504 cases of cancer were downloaded from The Cancer Genome Atlas (TCGA). B...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6529
更新日期:2018-09-01 00:00:00
abstract::By combining suppression subtractive hybridization and microarray to examine gene expressions between metastatic and non-metastatic non-small cell lung cancer (NSCLC), we have identified differential expression spectra of matrix metalloproteinases (MMP). Among MMPs, expressions of MMP-13, -14, -15 and -24 decreased, t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is the sixth most commonly diagnosed cancer worldwide. It has poor clinical outcome due to intrinsic or acquired drug resistance. Deregulation of both apoptosis and autophagy contributes to chemotherapy resistance and disease progression. A new member of the inhibitors of ...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2019.7082
更新日期:2019-05-01 00:00:00
abstract::We investigated the effects of combined treatment with 5-fluorouracil and apigenin on proliferation and apoptosis, as well as the underlying mechanism, in human breast cancer MDA-MB-453 cells. The MDA-MB-453 cells, which have been shown to overexpress ErbB2, were resistant to 5-fluorouracil; 5-fluorouracil exhibited a...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000598
更新日期:2009-12-01 00:00:00
abstract::In this study, a total of 108 patients with primary colorectal cancer who underwent hybrid 2-port hand-assisted laparoscopic surgery (HALS) were classified as 58 patients with colon cancer and 50 patients with rectal cancer. The mean operating time, mean blood loss, postoperative complications, and mean postoperative ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000514
更新日期:2009-10-01 00:00:00
abstract::Tumor growth is accelerated by induction of angiogenesis regulated by many cytokines and growth factors. In a tumor mass, various angiogenic factors and their receptors are simultaneously expressed and the overlapped expressions of various factors may contribute to the aggressive growth of tumor. However, the possible...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract::It is well known that crucifers have antitumor effects and 3,3'-diindolylmethane (DIM) is one of the major bioactive components, and the associated molecular mechanisms in a short-term high-dose manner are widely discussed. However, the antitumor effects of DIM in a long-term low-dose manner in nasopharyngeal carcinom...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4428
更新日期:2016-02-01 00:00:00
abstract::Novel agents need to be developed to overcome the limitations of the current melanoma therapeutics. Atractylenolide I (AT-I) is a sesquiterpene compound isolated from atractylodis macrocephalae rhizoma. Previous findings demonstrated that AT-I exhibited cytotoxic action in melanoma cells. However, the molecular mechan...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4111
更新日期:2015-09-01 00:00:00
abstract::Subsequently to the publication of this paper, the authors have realized that the affilitation address omitted the name of the university at which this research was conducted (Capital Medical University). Therefore, the author affiliations for this paper should have appeared as follows (changes are highlighted in bold...
journal_title:Oncology reports
pub_type: 杂志文章,已发布勘误
doi:10.3892/or.2019.7385
更新日期:2019-12-01 00:00:00
abstract::Quercetin is a potent cancer therapeutic agent and dietary antioxidant present in fruit and vegetables. Quercetin prevents tumor proliferation by inducing cell cycle arrest and is a well known cancer therapeutic agent and autophagy mediator. We investigated whether quercetin enhances TRAIL-induced tumor cell death and...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3991
更新日期:2015-07-01 00:00:00
abstract::WNT5B is a member of the WNT family that has been reported to be overexpressed in a variety of cancer cell lines and tissues, including colorectal cancer (CRC). However, the potential roles of WNT5B in tumorigenesis have not been reported. In the present study, the WNT5B gene was transfected into CRC cells and generat...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4772
更新日期:2016-07-01 00:00:00
abstract::Fecal microRNAs (miRNAs) are increasingly explored as non-invasive markers of colorectal cancer (CRC). However, its holistic profile in Asian CRC patients remains elusive. In the present study, the global human fecal miRNAs in Asian Chinese CRC patients was assayed to elucidate novel diagnostic fecal markers. Addition...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3193
更新日期:2014-07-01 00:00:00
abstract::High-mobility group protein box 1 (HMGB1) released from dying tumor cells promotes tumor progression, on the other hand HMGB1 activates dendritic cells and triggers anti-neoplastic response of T-cells in chemotherapy and radiotherapy. HMGB1 expression is up-regulated in many kinds of tumors. To investigate HMGB1 expre...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000468
更新日期:2009-09-01 00:00:00
abstract::MicroRNAs (miRNAs) are small non-coding RNAs that are involved in tumor initiation and development by suppressing target gene expression. miRNA-154 has been shown to be important in tumorigenesis in many types of cancers. However, its role in osteosarcoma (OS) remains unknown. In the present study, we focused on the r...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4495
更新日期:2016-03-01 00:00:00
abstract::Programmed cell death 2 (PDCD2) is a highly conserved nuclear protein, and aberrant PDCD2 expression alters cell apoptosis. The present study aimed to investigate PDCD2 expression in gastric cancer. Tissue specimens from 34 gastric cancer patients were collected for analysis of PDCD2 expression using immunohistochemis...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3551
更新日期:2015-01-01 00:00:00
abstract::Gene therapy mediated by murine leukemia virus (MLV)-based replicating retrovirus vector (RRV) was previously proven to be highly effective in tumor cell killing, resulting in significant suppression of tumor growth in vivo. Recently, we developed a different form of RRV which is derived from another retrovirus, gibbo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1789
更新日期:2012-07-01 00:00:00
abstract::In 72 patients without occult neoplastic cells (ONCs) in their lymph node sinuses, the 5-year relapse-free survival (RFS) rate and overall survival (OS) rate were 71.3% and 69.2%, respectively. In 33 patients with ONCs, the 5-year RFS rate and OS rate were 33.9% and 31.3%, respectively. There was a marked difference o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-11-01 00:00:00
abstract::Although ascorbate (Vitamin C) has been shown to inhibit cell growth and induce cell death in variety of cancer cells, results reported in other studies are inconsistent with this conclusion. It was previously reported that ascorbate induces apoptosis in human breast cancer cells. However, the molecular mechanism for ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-10-01 00:00:00
abstract::The deficiencies of nucleotide excision repair (NER) factors are involved in rare genetic diseases such as xeroderma pigmentosum (XP) with increased risk of developing cancer on sun-exposed areas of the skin. However, the abnormality of NER factors in human sporadic carcinoma remains unclear. Loss of heterozygosity (L...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.8.5.1133
更新日期:2001-09-01 00:00:00
abstract::Aquaporins (AQPs) are a family of small, integral membrane proteins that have been shown to play an important role in tumor development and metastasis. Several studies have demonstrated that expression of AQP3 contributes to the enhanced migration of epithelial cells and is related to differentiation, metastasis and v...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2144
更新日期:2013-02-01 00:00:00