Abstract:
:Topoisomerase II, purified from chicken erythrocytes, was reacted with a large number of different DNA fragments and cleavages were catalogued in the presence and absence of drugs that stabilize the cleavage intermediate. Cleavages were sequenced to derive a consensus for topoisomerase II that predicts catalytic sites. The consensus is: (sequence; see text) where N is any base and cleavage occurs at the indicated mark between -1 and +1. The consensus accurately predicts topoisomerase II sites in vitro. This consensus is not closely related to the Drosophila consensus sequence, but the two enzymes show some similarities in site recognition. Topoisomerase II purified from human placenta cleaves DNA sites that are essentially identical to the chicken enzyme, suggesting that vertebrate type II enzymes share a common catalytic sequence. Both viral and tissue specific enhancers contain sites sharing strong homology to the consensus and endogenous topoisomerase II recognizes some of these sites in vivo.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Spitzner JR,Muller MTdoi
10.1093/nar/16.12.5533subject
Has Abstractpub_date
1988-06-24 00:00:00pages
5533-56issue
12eissn
0305-1048issn
1362-4962journal_volume
16pub_type
杂志文章abstract::Wybutine (Ywye), situated next to the 3'-side of the anticodon of tRNAPhe from Saccharomyces cerevisiae, can be photo-crosslinked to mRNA when bound to Escherichia coli ribosomes. Crosslinking can be obtained with poly(U) as well as with oligonucleotides such as pAUGUUU or p(U)6. In order to identify the site of react...
journal_title:Nucleic acids research
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doi:10.1093/nar/12.21.8181
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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doi:10.1093/nar/gkm661
更新日期:2007-01-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2014-01-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2013-11-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2007-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1008
更新日期:2015-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2018-07-02 00:00:00
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journal_title:Nucleic acids research
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更新日期:2008-10-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1110
更新日期:2012-03-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr290
更新日期:2011-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.18.6341
更新日期:1983-09-24 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.14.5545
更新日期:1987-07-24 00:00:00
abstract::Transcription of tRNA-encoding genes by RNA polymerase (Pol) III requires the six-subunit general transcription factor IIIC that uses subcomplexes τA and τB to recognize two gene-internal promoter elements named A- and B-box. The Schizosaccharomyces pombe τA subcomplex comprises subunits Sfc1, Sfc4 and Sfc7. The cryst...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-10-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2015-10-30 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt338
更新日期:2013-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg681
更新日期:2003-08-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg897
更新日期:2003-12-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2005-01-31 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2002-09-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1986-08-26 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2010-03-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章,评审
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更新日期:2016-09-19 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gni118
更新日期:2005-07-26 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn218
更新日期:2008-07-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2014-12-16 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnj017
更新日期:2006-02-07 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm603
更新日期:2007-01-01 00:00:00