Molecular characterization of the MT-family of dispersed middle-repetitive DNA in rodent genomes.

abstract：:Through a computer search of DNA databases, we have identified the homologs of the mgU6-47 snoRNA gene from the yeast Schizosaccharomyces pombe, the fly Drosophila melanogaster and human. The three box C/D-containing snoRNA genes showed no significant similarity in their sequences except for an 11 nt long complementar...

journal_title：Nucleic acids research

authors： Zhou H,Chen YQ,Du YP,Qu LH

更新日期：2002-02-15 00:00:00

abstract：:A newly discovered group of spherical plant viruses contains a bipartite genome consisting of a single-strand linear RNA molecule (RNA 1, Mr 1.5 x 10(6) ), and a single-strand, covalently closed circular viroid-like RNA molecule (RNA 2, Mr approximately 125,000). The nucleotide sequences of the RNA 2 of two of these, ...

journal_title：Nucleic acids research

authors： Haseloff J,Symons RH

更新日期：1982-06-25 00:00:00

abstract：:In Xenopus oocytes, progesterone stimulates the cytoplasmic polyadenylation and resulting translational activation of c-mos mRNA, which is necessary for the induction of oocyte maturation. Although details of the biochemistry of polyadenylation are beginning to emerge, the mechanism by which 3' poly(A) addition stimul...

journal_title：Nucleic acids research

authors： Kuge H,Brownlee GG,Gershon PD,Richter JD

更新日期：1998-07-01 00:00:00

abstract：:The Histone Database (HDB) is an annotated and searchable collection of all full-length sequences and structures of histone and non-histone proteins containing the histone fold motif. These sequences are both eukaryotic and archaeal in origin. Several new histone fold-containing proteins have been identified, includin...

journal_title：Nucleic acids research

authors： Sullivan SA,Aravind L,Makalowska I,Baxevanis AD,Landsman D

更新日期：2000-01-01 00:00:00

abstract：:The RESID Database is a comprehensive collection of annotations and structures for protein post-translational modifications including N-terminal, C-terminal and peptide chain cross-link modifications. The RESID Database includes systematic and frequently observed alternate names, Chemical Abstracts Service registry nu...

journal_title：Nucleic acids research

authors： Garavelli JS,Hou Z,Pattabiraman N,Stephens RM

更新日期：2001-01-01 00:00:00

abstract：:Conformations of 8-bromo-2'-[unk]-triisopropylbenzenesulfonyladenosine ([unk]) and its 3'-[unk]-isomer ([unk]) in solution have been determined by the use of intramolecular nuclear Overhauser effects in (1)H NMR spectroscopy. Compound [unk] has been proved to have a conformation in which the adenosine and benzene ring...

journal_title：Nucleic acids research

authors： Ueyama M,Tori K,Ikehara M,Kaneko M

更新日期：1974-06-01 00:00:00

abstract：:The template requirements for efficient adenovirus DNA replication were studied in vitro in a reconstituted system with cloned DNA fragments, containing the Ad2 origin region, as templates. Replication is enhanced by nuclear factor I, a cellular protein that binds specifically to the Ad2 origin. This stimulation is sh...

journal_title：Nucleic acids research

authors： de Vries E,van Driel W,Tromp M,van Boom J,van der Vliet PC

更新日期：1985-07-11 00:00:00

abstract：:CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated) systems allow bacteria to adapt to infection by acquiring 'spacer' sequences from invader DNA into genomic CRISPR loci. Cas proteins use RNAs derived from these loci to target cognate sequences for destruction through CRISPR inter...

journal_title：Nucleic acids research

authors： Xue C,Seetharam AS,Musharova O,Severinov K,Brouns SJ,Severin AJ,Sashital DG

更新日期：2015-12-15 00:00:00

abstract：:The nature of mammalian origins of DNA replication remains controversial and this is primarily because two-dimensional gel replicon mapping techniques have identified broad zones of replication initiation whereas several other techniques, such as quantitative PCR, have disclosed more discrete sites of initiation at th...

journal_title：Nucleic acids research

authors： Toledo F,Baron B,Fernandez MA,Lachagès AM,Mayau V,Buttin G,Debatisse M

更新日期：1998-05-15 00:00:00

abstract：:The accumulation of somatic mitochondrial DNA (mtDNA) mutations contributes to the pathogenesis of human disease. Currently, mitochondrial mutations are largely considered results of inaccurate processing of its heavily damaged genome. However, mainly from a lack of methods to monitor mtDNA mutations with sufficient s...

journal_title：Nucleic acids research

authors： Valente WJ,Ericson NG,Long AS,White PA,Marchetti F,Bielas JH

更新日期：2016-10-14 00:00:00

abstract：:Multiple myeloma, the second most frequent hematologic tumor after lymphomas, is an incurable cancer. Recent sequencing efforts have identified the ribonuclease DIS3 as one of the most frequently mutated genes in this disease. DIS3 represents the catalytic subunit of the exosome, a macromolecular complex central to th...

journal_title：Nucleic acids research

authors： Segalla S,Pivetti S,Todoerti K,Chudzik MA,Giuliani EC,Lazzaro F,Volta V,Lazarevic D,Musco G,Muzi-Falconi M,Neri A,Biffo S,Tonon G

更新日期：2015-05-26 00:00:00

abstract：:A series of unusual folding regions (UFR) immediately 3' to the cleavage site of the outer membrane protein (OMP) and transmembrane protein (TMP) were detected in the envelope gene RNA of the human immunodeficiency virus (HIV-1, HIV-2) and simian immunodeficiency virus (SIV) by an extensive Monte Carlo simulation. The...

journal_title：Nucleic acids research

authors： Le SY,Malim MH,Cullen BR,Maizel JV

更新日期：1990-03-25 00:00:00

abstract：:Recently, we developed a mass DNA-mediated transformation technique for the ciliated protozoan Tetrahymena thermophila that introduces transforming DNA by electroporation into conjugating cells. Other studies demonstrated that a neomycin resistance gene flanked by Tetrahymena H4-I gene regulatory sequences transformed...

journal_title：Nucleic acids research

authors： Gaertig J,Gu L,Hai B,Gorovsky MA

更新日期：1994-12-11 00:00:00

abstract：:Furvina®, also denominated G1 (MW 297), is a synthetic nitrovinylfuran [2-bromo-5-(2-bromo-2-nitrovinyl)-furan] antibiotic with a broad antimicrobial spectrum. An ointment (Dermofural®) containing G1 as the only active principle is currently marketed in Cuba and successfully used to treat dermatological infections. He...

journal_title：Nucleic acids research

authors： Fabbretti A,Brandi L,Petrelli D,Pon CL,Castañedo NR,Medina R,Gualerzi CO

更新日期：2012-11-01 00:00:00

abstract：:A temperature-sensitive mutant of Escherichia coli affected in the alpha subunit of RNA polymerase has been investigated. Gene mapping and complementation experiments placed the mutation to temperature-sensitivity within the alpha operon at 72 min. on the bacterial chromosome. The rate of RNA synthesis in vivo and the...

journal_title：Nucleic acids research

authors： Mehrpouyan M,Champney WS

更新日期：1990-06-25 00:00:00

abstract：:MicroRNA (miRNA) 5'-isoforms, or 5'-isomiRs, are small-RNA species that originate from the same genomic loci as the major miRNAs with their 5' ends shifted from the 5' ends of the miRNAs by a few nucleotides. Although 5'-isomiRs have been reported, their origins, properties and potential functions remain to be examine...

journal_title：Nucleic acids research

authors： Xia J,Zhang W

更新日期：2014-02-01 00:00:00

abstract：:PrimPol is a primase-polymerase involved in nuclear and mitochondrial DNA replication in eukaryotic cells. Although PrimPol is predicted to possess an archaeo-eukaryotic primase and a UL52-like zinc finger domain, the role of these domains has not been established. Here, we report that the proposed zinc finger domain ...

journal_title：Nucleic acids research

authors： Keen BA,Jozwiakowski SK,Bailey LJ,Bianchi J,Doherty AJ

更新日期：2014-05-01 00:00:00

abstract：:The high-mobility-group A2 protein (HMGA2) plays important functional roles in transcriptional regulation, DNA replication and chromatin structure. In this study, the effect of histone deacetylase inhibition on the transcriptional activity of the Hmga2 gene was investigated in vivo both at the endogenous gene level an...

journal_title：Nucleic acids research

authors： Ferguson M,Henry PA,Currie RA

更新日期：2003-06-15 00:00:00

abstract：:The transcriptional promoter of satellite 2 from the eastern newt, Notophthalmus viridescens, was analyzed by assaying the activity of deleted or mutated satellite 2 clones in Xenopus laevis oocytes. Two elements in the promoter were found to be important for transcription. These elements have sequences that are simil...

journal_title：Nucleic acids research

authors： Coats SR,Zhang Y,Epstein LM

更新日期：1994-11-11 00:00:00

abstract：:The results of differential expression analyses provide scientists with hundreds to thousands of differentially expressed genes that need to be interpreted in light of the biology of the specific system under study. This requires mapping the genes to functional classifications that can be, for example, the KEGG pathwa...

journal_title：Nucleic acids research

authors： D'Andrea D,Grassi L,Mazzapioda M,Tramontano A

更新日期：2013-07-01 00:00:00

abstract：:Pulsed field gel electrophoresis was used to determine the location of the tumour necrosis factor (TNF) alpha and beta genes. They were shown to be linked to the HLA-B locus; analogous to their location in mouse, between the complement (class III) region and H-2D. However, the distance between the TNF genes and the cl...

journal_title：Nucleic acids research

authors： Inoko H,Trowsdale J

更新日期：1987-11-11 00:00:00

abstract：:The σ subunit of bacterial RNA polymerase (RNAP) has been implicated in all steps of transcription initiation, including promoter recognition and opening, priming of RNA synthesis, abortive initiation and promoter escape. The post-promoter-recognition σ functions were proposed to depend on its conserved region σ3.2 th...

journal_title：Nucleic acids research

authors： Pupov D,Kuzin I,Bass I,Kulbachinskiy A

更新日期：2014-04-01 00:00:00

abstract：:Specific targeting of protein–nucleic acid interactions is an area of current interest, for example, in the regulation of gene-expression. Most transcription factor proteins bind in the DNA major groove; however, we are interested in an approach using small molecules to target the minor groove to control expression by...

journal_title：Nucleic acids research

authors： Laughlin-Toth S,Carter EK,Ivanov I,Wilson WD

更新日期：2017-02-17 00:00:00

abstract：:E. coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief but intense electrical fields of exponential decay waveform (electroporation). We have obtained 10(9) to 10(10) transformants/micrograms with strains LE392 and DH5 alpha, and plasmids pUC18 and pBR329. The proce...

journal_title：Nucleic acids research

authors： Dower WJ,Miller JF,Ragsdale CW

更新日期：1988-07-11 00:00:00

abstract：:The nucleotide sequence of a region located on the adenovirus type 5 genome between coordinates 59.9 and 62.5 has been determined. This region comprises the 3'-termini of mRNAs coding for the hexon and the DNA binding protein. The sequence reveals the presence of an open reading frame in r-strand transcripts downstrea...

journal_title：Nucleic acids research

authors： Kruijer W,van Schaik FM,Sussenbach JS

更新日期：1980-12-20 00:00:00

abstract：:Recent data showed that p53 stimulates the expression of genes encoding not only pro- but also antioxidant enzymes. It was suggested that antioxidant genes could be induced under physiologic levels of stress while the prooxidant ones respond to higher level of stress. Results presented in this article illustrate an ad...

journal_title：Nucleic acids research

authors： Meiller A,Alvarez S,Drané P,Lallemand C,Blanchard B,Tovey M,May E

更新日期：2007-01-01 00:00:00

abstract：:Human Protein Reference Database (HPRD) (http://www.hprd.org) was developed to serve as a comprehensive collection of protein features, post-translational modifications (PTMs) and protein-protein interactions. Since the original report, this database has increased to >20 000 proteins entries and has become the largest...

journal_title：Nucleic acids research

authors： Mishra GR,Suresh M,Kumaran K,Kannabiran N,Suresh S,Bala P,Shivakumar K,Anuradha N,Reddy R,Raghavan TM,Menon S,Hanumanthu G,Gupta M,Upendran S,Gupta S,Mahesh M,Jacob B,Mathew P,Chatterjee P,Arun KS,Sharma S,Chand

更新日期：2006-01-01 00:00:00

abstract：:DNA triplet repeats, 5'-d(CTG)n and 5'-d(CAG)n, are present in genes which have been implicated in several neurodegenerative disorders. To investigate possible stable structures formed by these repeating sequences, we have examined d(CTG)n, d(CAG)n and d(CTG).d(CAG)n (n = 2 and 3) using NMR and UV optical spectroscopy...

journal_title：Nucleic acids research

authors： Smith GK,Jie J,Fox GE,Gao X

更新日期：1995-11-11 00:00:00

abstract：:Lateral ribosomal stalk is responsible for binding and recruiting translation factors during protein synthesis. The eukaryotic stalk consists of one P0 protein with two copies of P1•P2 heterodimers to form a P0(P1•P2)₂ pentameric P-complex. Here, we have solved the structure of full-length P1•P2 by nuclear magnetic re...

journal_title：Nucleic acids research

authors： Lee KM,Yusa K,Chu LO,Yu CW,Oono M,Miyoshi T,Ito K,Shaw PC,Wong KB,Uchiumi T

更新日期：2013-10-01 00:00:00

abstract：:We report here the construction of engineered endonuclease database (EENdb) (http://eendb.zfgenetics.org/), a searchable database and knowledge base for customizable engineered endonucleases (EENs), including zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs). EENs are artificial...

journal_title：Nucleic acids research

authors： Xiao A,Wu Y,Yang Z,Hu Y,Wang W,Zhang Y,Kong L,Gao G,Zhu Z,Lin S,Zhang B

更新日期：2013-01-01 00:00:00