Abstract：
:Bone marrow and adipose tissue represent the two most commonly exploited sources of adult mesenchymal stem cells for musculoskeletal applications. Unfortunately the sampling of bone marrow and fat tissue is invasive and does not always lead to a sufficient number of cells. The present study describes a novel sampling method based on microbiopsy of skeletal muscle in man, pigs, dogs and horses. The process includes explant of the sample, Percoll density gradient for isolation and subsequent culture of the cells. We further characterized the cells and identified their clonogenic and immunomodulatory capacities, their immune-phenotyping behavior and their capability to differentiate into chondroblasts, osteoblasts and adipocytes. In conclusion, this report describes a novel and easy-to-use technique of skeletal muscle-derived mesenchymal stem cell harvest, culture, characterization. This technique is transposable to a multitude of different animal species.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ceusters J,Lejeune JP,Sandersen C,Niesten A,Lagneaux L,Serteyn Ddoi
10.1038/s41598-017-00803-7subject
Has Abstractpub_date
2017-04-06 00:00:00pages
696issue
1issn
2045-2322pii
10.1038/s41598-017-00803-7journal_volume
7pub_type
杂志文章abstract：:We proposed a simple model for generation of controllable ultraslow optical solitons of a weak probe laser light in a degenerated two-level atomic medium under electromagnetically induced transparency assisted by a magnetic field. It is shown that bright and dark optical solitons can be formed from a probe light with ...
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pub_type： 杂志文章
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pub_type： 历史文章,杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章,随机对照试验
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