Abstract：
:Mapping the location of bound cellulase enzymes provides information on the micro-scale distribution of amenable and recalcitrant sites in pretreated woody biomass for biofuel applications. The interaction of a fluorescently labelled cellulase enzyme cocktail with steam-exploded pine (SEW) was quantified using confocal microscopy. The spatial distribution of Dylight labelled cellulase was quantified relative to lignin (autofluorescence) and cellulose (Congo red staining) by measuring their colocalisation using Pearson correlations. Correlations were greater in cellulose-rich secondary cell walls compared to lignin-rich middle lamella but with significant variations among individual biomass particles. The distribution of cellulose in the pretreated biomass accounted for 30% of the variation in the distribution of enzyme after correcting for the correlation between lignin and cellulose. For the first time, colocalisation analysis was able to quantify the spatial distribution of amenable and recalcitrant sites in relation to the histochemistry of cellulose and lignin. This study will contribute to understanding the role of pretreatment in enzymatic hydrolysis of recalcitrant softwood biomass.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Donaldson L,Vaidya Adoi
10.1038/srep44386subject
Has Abstractpub_date
2017-03-10 00:00:00pages
44386issn
2045-2322pii
srep44386journal_volume
7pub_type
杂志文章abstract：:Soil moisture is an important variable for land-climate and hydrological interactions. To investigate emergent large-scale, long-term interactions between soil moisture and other key hydro-climatic variables (precipitation, actual evapotranspiration, runoff, temperature), we analyze monthly values and anomalies of the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61621-y
更新日期：2020-03-16 00:00:00
abstract：:Cerebral aneurysms are abnormal focal dilatations of arterial vessel walls with pathological vessel structure alterations. Sudden rupture can lead to a subarachnoid hemorrhage, which is associated with a high mortality. Therefore, the origin of cerebral aneurysms as well as the progression to the point of rupture need...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69222-5
更新日期：2020-07-23 00:00:00
abstract：:Plants are continuously exposed to myriad pathogen stresses. However, the molecular mechanisms by which these stress signals are perceived and transduced are poorly understood. In this study, the maize gene GRMZM2G315431 was identified to be highly inducible by Rhizoctonia solani infection, suggesting that the promote...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42059
更新日期：2017-02-06 00:00:00
abstract：:The microRNA (miR)-183/96/182 cluster plays important roles in the development and functions of sensory organs, including the inner ear. Point-mutations in the seed sequence of miR-96 result in non-syndromic hearing loss in both mice and humans. However, the lack of a functionally null mutant has hampered the evaluati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36894-z
更新日期：2018-12-21 00:00:00
abstract：:Awe, a complex emotion composed by the appraisal components of vastness and need for accommodation, is a profound and often meaningful experience. Despite its importance, psychologists have only recently begun empirical study of awe. At the experimental level, a main issue concerns how to elicit high intensity awe exp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01242-0
更新日期：2017-04-27 00:00:00
abstract：:Acrolein, a highly toxic α, β-unsaturated aldehyde, has been a longstanding key biomarker associated with a range of disorders related to oxidative stresses. One of the most promising methods for detecting acrolein involves the use of antibodies that can recognize the acrolein-lysine conjugate, 3-formyl-3, 4-dehydropi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35872
更新日期：2016-10-26 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-67913-7
更新日期：2020-06-30 00:00:00
abstract：:The structural characterization correlated to the processing control of hierarchical structure of planar heterojunction perovskite layer is still incomplete due to the limitations of conventional microscopy and X-ray diffraction. This present study performed the simultaneously grazing-incidence small-angle scattering ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13657
更新日期：2015-09-04 00:00:00
abstract：:Fluorescence-guided surgery using 5-aminolevulinic acid (5-ALA) is now a widely-used modality for glioblastoma (GBM) treatment. However, intratumoral heterogeneity of fluorescence intensity may reflect different onco-metabolic programs. Here, we investigated the metabolic mechanism underlying the heterogeneity of 5-AL...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12557-3
更新日期：2017-09-22 00:00:00
abstract：:Large amounts of anthropogenic CO2 in the atmosphere are taken up by the ocean, which leads to 'ocean acidification' (OA). In addition, the increasing application of nanoparticles inevitably leads to their increased release into the aquatic environment. However, the impact of OA on the bioaccumulation of nanoparticles...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40047-1
更新日期：2019-03-05 00:00:00
abstract：:This study sought to evaluate the association of 28 single nucleotide polymorphisms (SNPs) within NFKB and inflammasome pathway genes with the risk of rheumatoid arthritis (RA) and response to TNF inhibitors (TNFi). We conducted a case-control study in a European population of 1194 RA patients and 1328 healthy control...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61331-5
更新日期：2020-03-09 00:00:00
abstract：:Hfq is a pleiotropic regulator that mediates several aspects of bacterial RNA metabolism. The protein notably regulates translation efficiency and RNA decay in Gram-negative bacteria, usually via its interaction with small regulatory RNA. Besides these RNA-related functions, Hfq has also been described as one of the n...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35060-9
更新日期：2018-11-14 00:00:00
abstract：:The future Internet is very likely the mixture of all-optical Internet with low power consumption and quantum Internet with absolute security guaranteed by the laws of quantum mechanics. Photons would be used for processing, routing and com-munication of data, and photonic transistor using a weak light to control a st...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45582
更新日期：2017-03-28 00:00:00
abstract：:In the present work, linoleic acid and oleic acid were isolated from Indonesian corn oil and palm oil and they were used to prepare monoacylglycerol derivatives as the antibacterial agent. Indonesian corn oil contains 57.74% linoleic acid, 19.88% palmitic acid, 11.84% oleic acid and 3.02% stearic acid. While Indonesia...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47373-4
更新日期：2019-07-29 00:00:00
abstract：:Combination targeted therapy is commonly used to treat acute myeloid leukemia (AML) patients, particularly in refractory/relapse (RR) population. However, concerns have been raised regarding the safety and patient tolerance of combination chemotherapy. It is critical to choose the appropriate treatment for precision t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40361
更新日期：2017-01-18 00:00:00
abstract：:Smad1, Smad5 and Smad9 (also known as Smad8) are activated by phosphorylation by bone morphogenetic protein (BMP)-bound type I receptor kinases. We examined the role of Smad1, Smad5, and Smad9 by creating constitutively active forms (Smad(DVD)). Transcriptional activity of Smad9(DVD) was lower than that of Smad1(DVD) ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07596
更新日期：2014-12-23 00:00:00
abstract：:In this paper we applied transfer learning techniques for image recognition, automatic categorization, and labeling of nanoscience images obtained by scanning electron microscope (SEM). Roughly 20,000 SEM images were manually classified into 10 categories to form a labeled training set, which can be used as a referenc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13565-z
更新日期：2017-10-16 00:00:00
abstract：:There is a well-established relationship between joint hypermobility and anxiety in humans, that has not previously been investigated in other species. A population of 5575 assistance dogs were scored for both hip hypermobility and 13 behaviour characteristics using previously validated methods. Our results suggest a ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45096-0
更新日期：2019-06-13 00:00:00
abstract：:Culture medium of mesenchymal stromal cells (MSCs) is usually supplemented with either human platelet lysate (HPL) or fetal calf serum (FCS). Many studies have demonstrated that proliferation and cellular morphology are affected by these supplements - it is therefore important to determine if they favor outgrowth of d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05207-1
更新日期：2017-07-11 00:00:00
abstract：:Among abundant reconstructions of Holocene climate in Europe, only a handful has addressed winter conditions, and most of these are restricted in length and/or resolution. Here we present a record of late autumn through early winter air temperature and moisture source changes in East-Central Europe for the Holocene, b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01397-w
更新日期：2017-04-26 00:00:00
abstract：:Remarkable improvements in both structural and optical properties of wafer-scale hexagonal boron nitride (h-BN) films grown by metal-organic chemical vapor deposition (MOCVD) enabled by high-temperature post-growth annealing is presented. The enhanced crystallinity and homogeneity of the MOCVD-grown h-BN films grown a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47093-9
更新日期：2019-07-22 00:00:00
abstract：:Core flood and field tests have demonstrated that decreasing injection water salinity increases oil recovery from sandstone reservoirs. However, the microscopic mechanism behind the effect is still under debate. One hypothesis is that as salinity decreases, expansion of the electrical double layer decreases attraction...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09933
更新日期：2015-04-22 00:00:00
abstract：:Increased proteolytic activity has been widely associated with skeletal muscle atrophy. However, elevated proteolysis is also critical for the maintenance of cellular homeostasis by disposing cytotoxic proteins and non-functioning organelles. We recently demonstrated that exercise activates autophagy and re-establishe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30365-1
更新日期：2018-08-07 00:00:00
abstract：:Cystic fibrosis-related diabetes (CFRD) worsens CF lung disease leading to early mortality. Loss of beta cell area, even without overt diabetes or pancreatitis is consistently observed. We investigated whether short-term CFTR inhibition was sufficient to impact islet morphology and function in otherwise healthy mice. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47745-w
更新日期：2019-08-02 00:00:00
abstract：:Cry1A insecticidal toxins bind sequentially to different larval gut proteins facilitating oligomerization, membrane insertion and pore formation. Cry1Ac interaction with cadherin triggers oligomerization. However, a mutation in an ABC transporter gene (ABCC2) is linked to Cry1Ac resistance in Plutella xylostella. Cry1...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02545-y
更新日期：2017-05-24 00:00:00
abstract：:The Saccharomyces cerevisiae kinesin-5 Cin8 performs essential mitotic functions in spindle assembly and anaphase B spindle elongation. Recent work has shown that Cin8 is a bi-directional motor which moves towards the minus-end of microtubules (MTs) under high ionic strength (IS) conditions and changes directionality ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25597
更新日期：2016-05-24 00:00:00
abstract：:Bio-functionalized nanoparticles with semiconducting/metallic core encapsulated in a bio- or bio-derived materials are promising for applications in biology and especially in cancer diagnostic and healing. In this report, we report a facile, single-step, first-time synthesis and in-situ functionalization strategy for ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18629
更新日期：2015-12-21 00:00:00
abstract：:Transcriptionally silent heterochromatin is associated with repetitive DNA. It is poorly understood whether and how heterochromatin differs between different organisms and whether its structure can be remodelled in response to environmental signals. Here, we address this question by analysing the chromatin state assoc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22989
更新日期：2016-03-14 00:00:00
abstract：:The estrogen signaling pathway has been reported to modulate prostate cancer (PCa) progression through the activity of estrogen receptors α and β (ERα and ERβ). Given that selective estrogen receptor modulators (SERMs) are used to treat breast cancer, ERs have been proposed as attractive therapeutic targets in PCa. Ho...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60844-3
更新日期：2020-03-04 00:00:00
abstract：:Nature disasters and terrorist attacks have occurred globally in recent years. Posttraumatic stress disorder (PTSD) has gained increasing attention, but its incidence and comorbidities in the general population are different from those inside the disaster areas. The present study estimated incident PTSD and comorbid d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41276
更新日期：2017-01-27 00:00:00
