当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > A Reduction-Based Sensor for Acrolein Conjugates with the Inexpensive Nitrobenzene as an Alternative to Monoclonal Antibody.

A Reduction-Based Sensor for Acrolein Conjugates with the Inexpensive Nitrobenzene as an Alternative to Monoclonal Antibody.

Abstract：

:Acrolein, a highly toxic α, β-unsaturated aldehyde, has been a longstanding key biomarker associated with a range of disorders related to oxidative stresses. One of the most promising methods for detecting acrolein involves the use of antibodies that can recognize the acrolein-lysine conjugate, 3-formyl-3, 4-dehydropiperidines (FDP), within oxidatively stressed cells and tissues from various disease states. We have uncovered here that FDP could reduce nitroarenes in high yields at 100 °C in the presence of excess CaCl2 as a Lewis acid promoter. This unique transformation allowed for the development of a de novo method for detecting levels of FDPs generated from proteins in urine or blood serum samples. Thus we successfully converted a non-fluorescent and inexpensive 4-nitrophthalonitrile probe to the corresponding fluorescent aniline, thereby constituting the concept of fluorescent switching. Its sensitivity level (0.84 nmol/mL) is more than that of ELISA assays (3.13 nmol/mL) and is already equally reliable and reproducible at this early stage of development. More importantly, this method is cost effective and simple to operate, requiring only mixing of samples with a kit solution. Our method thus possesses potential as a future alternative to the more costly and operatively encumbered conventional antibody-based methods.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Takamatsu M,Fukase K,Oka R,Kitazume S,Taniguchi N,Tanaka K

doi

10.1038/srep35872

subject

Has Abstract

pub_date

2016-10-26 00:00:00

pages

35872

issn

2045-2322

pii

srep35872

journal_volume

6

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Optimization of a reduced enzymatic reaction cascade for the production of L-alanine.

    abstract：:Cell-free enzymatic reaction cascades combine the advantages of well-established in vitro biocatalysis with the power of multi-step in vivo pathways. The absence of a regulatory cell environment enables direct process control including methods for facile bottleneck identification and process optimization. Within this ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-48151-y

    authors： Gmelch TJ,Sperl JM,Sieber V

    更新日期：2019-08-13 00:00:00

  • Functional interplay between the transcription factors USF1 and PDX-1 and protein kinase CK2 in pancreatic β-cells.

    abstract：:Glucose homeostasis is regulated by insulin, which is produced in the β-cells of the pancreas. The synthesis of insulin is controlled by several transcription factors including PDX-1, USF1 and USF2. Both, PDX-1 and USF1 were identified as substrates for protein kinase CK2. Here, we have analysed the interplay of PDX-1...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-16590-0

    authors： Spohrer S,Groß R,Nalbach L,Schwind L,Stumpf H,Menger MD,Ampofo E,Montenarh M,Götz C

    更新日期：2017-11-27 00:00:00

  • Varied Length Stokes Shift BODIPY-Based Fluorophores for Multicolor Microscopy.

    abstract：:Multicolor microscopy tools necessary to localize and visualize the complexity of subcellular systems are limited by current fluorophore technology. While commercial fluorophores cover spectral space from the ultraviolet to the near infrared region and are optimized for conventional bandpass based fluorescence microsc...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-22892-8

    authors： Bittel AM,Davis AM,Wang L,Nederlof MA,Escobedo JO,Strongin RM,Gibbs SL

    更新日期：2018-03-15 00:00:00

  • Differential depression of neuronal network activity by midazolam and its main metabolite 1-hydroxymidazolam in cultured neocortical slices.

    abstract：:The benzodiazepine midazolam is widely used in critical care medicine. Midazolam has a clinically active metabolite, 1-hydroxymidazolam. The contribution of 1-hydroxymidazolam to the effects of midazolam is controversial. The aim of the current study was to compare the actions of midazolam and 1-hydroxymidazolam on ne...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-03154-5

    authors： Balk M,Hentschke H,Rudolph U,Antkowiak B,Drexler B

    更新日期：2017-06-14 00:00:00

  • Mucosal-associated invariant T (MAIT) cells provide B-cell help in vaccinated and subsequently SIV-infected Rhesus Macaques.

    abstract：:Mucosal-associated invariant T (MAIT) cells help combat opportunistic infections. Thus, MAIT cells are of interest in HIV/SIV vaccination and infection. We investigated MAIT cell dynamics and function in rhesus macaque blood and bronchoalveolar lavage (BAL) following mucosal adenovirus (Ad)-SIV recombinant priming, in...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66964-0

    authors： Rahman MA,Ko EJ,Bhuyan F,Enyindah-Asonye G,Hunegnaw R,Helmold Hait S,Hogge CJ,Venzon DJ,Hoang T,Robert-Guroff M

    更新日期：2020-06-22 00:00:00

  • Drone exposure to the systemic insecticide Fipronil indirectly impairs queen reproductive potential.

    abstract：:A species that requires sexual reproduction but cannot reproduce is doomed to extinction. The important increasing loss of species emphasizes the ecological significance of elucidating the effects of environmental stressors, such as pesticides, on reproduction. Despite its special reproductive behavior, the honey bee ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep31904

    authors： Kairo G,Provost B,Tchamitchian S,Ben Abdelkader F,Bonnet M,Cousin M,Sénéchal J,Benet P,Kretzschmar A,Belzunces LP,Brunet JL

    更新日期：2016-08-23 00:00:00

  • Imaging local brain activity of multiple freely moving mice sharing the same environment.

    abstract：:Electrophysiological field potential dynamics have been widely used to investigate brain functions and related psychiatric disorders. Considering recent demand for its applicability to freely moving subjects, especially for animals in a group and socially interacting with each other, here we propose a new method based...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-43897-x

    authors： Inagaki S,Agetsuma M,Ohara S,Iijima T,Yokota H,Wazawa T,Arai Y,Nagai T

    更新日期：2019-05-16 00:00:00

  • Spectrally intense terahertz source based on triangular Selenium.

    abstract：:The intensity of a nonlinear terahertz (THz) source is primarily given by its spectral density. In this letter, we introduce triangular Selinium (Se) as a novel THz emitter and show numerically its superior properties to the currently used crystals for intense THz generation. The excellent phase matching enables the a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep08059

    authors： Shalaby M,Hauri CP

    更新日期：2015-01-27 00:00:00

  • Apolipoprotein E4 and Insulin Resistance Interact to Impair Cognition and Alter the Epigenome and Metabolome.

    abstract：:Apolipoprotein E4 (E4) and type 2 diabetes are major risk factors for cognitive decline and late onset Alzheimer's disease (AD). E4-associated phenotypes and insulin resistance (IR) share several features and appear to interact in driving cognitive dysfunction. However, shared mechanisms that could explain their overl...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep43701

    authors： Johnson LA,Torres ER,Impey S,Stevens JF,Raber J

    更新日期：2017-03-08 00:00:00

  • Three dimensional metal/N-doped nanoplate carbon catalysts for oxygen reduction, the reason for using a layered nanoreactor.

    abstract：:A layered nanoreactor (zinc hydroxide gallate/nitrate nanohybrid) has been designed as a nano-vessel to confine the gallate/nitrate reaction inside zinc hydroxide layers for production of metal/nitrogen-doped carbon catalysts. Metals (Fe2+, Co2+ and Ni2+) doped and bare zinc hydroxide nitrates (ZHN) were synthesized a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-21782-3

    authors： Yeganeh Ghotbi M,Javanmard A,Soleimani H

    更新日期：2018-02-21 00:00:00

  • Fluorometric Measurement of Individual Stomata Activity and Transpiration via a "Brush-on", Water-Responsive Polymer.

    abstract：:Much of atmospheric water originates from transpiration, the process by which plants release H2O from pores, known as stomata, that simultaneously intake CO2 for photosynthesis. Controlling stomatal aperture can regulate the extent of water transport in response to dynamic environmental factors including osmotic stres...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep32394

    authors： Seo M,Park DH,Lee CW,Jaworski J,Kim JM

    更新日期：2016-08-31 00:00:00

  • Key role of 15-LO/15-HETE in angiogenesis and functional recovery in later stages of post-stroke mice.

    abstract：:This study sought to clarify the effects of 15-lipoxygenase/15-hydroxyeicosatetraenoic acid in angiogenesis and neurological functional recovery after cerebral ischaemic stroke in mice. In vivo, we performed behavioural tests to determine functional recovery after stroke. Double immunofluorescence staining of CD31 and...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep46698

    authors： Wang D,Liu Y,Chen L,Li P,Qu Y,Zhu Y,Zhu Y

    更新日期：2017-04-24 00:00:00

  • Release of a liver anticancer drug, sorafenib from its PVA/LDH- and PEG/LDH-coated iron oxide nanoparticles for drug delivery applications.

    abstract：:The use of nanocarriers composed of polyethylene glycol- and polyvinyl alcohol-coated vesicles encapsulating active molecules in place of conventional chemotherapy drugs can reduce many of the chemotherapy-associated challenges because of the increased drug concentration at the diseased area in the body. The present s...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-76504-5

    authors： Ebadi M,Bullo S,Buskara K,Hussein MZ,Fakurazi S,Pastorin G

    更新日期：2020-12-09 00:00:00

  • Arbuscular mycorrhizal fungal community composition affected by original elevation rather than translocation along an altitudinal gradient on the Qinghai-Tibet Plateau.

    abstract：:Elucidating arbuscular mycorrhizal (AM) fungal responses to elevation changes is critical to improve understanding of microbial function in ecosystems under global asymmetrical climate change scenarios. Here we examined AM fungal community in a two-year reciprocal translocation of vegetation-intact soil blocks along a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep36606

    authors： Yang W,Zheng Y,Gao C,Duan JC,Wang SP,Guo LD

    更新日期：2016-11-09 00:00:00

  • The prevalence of sexual dysfunction in the female health care providers in Jeddah, Saudi Arabia.

    abstract：:The objective of this study was to determine the prevalence of sexual dysfunction in Saudi and non-Saudi female health care providers in Jeddah, Saudi Arabia. One -hundred twenty (60 Saudi and 60 non-Saudi) sexually active female health care professionals in Jeddah, Saudi Arabia, were anonymously surveyed using the En...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep07905

    authors： Rouzi AA,Sahly N,Sawan D,Kafy S,Alzaban F

    更新日期：2015-01-20 00:00:00

  • Revelation of early detection of co-seismic ionospheric perturbations in GPS-TEC from realistic modelling approach: Case study.

    abstract：:GPS-derived Total Electron Content (TEC) is an integrated quantity; hence it is difficult to relate the detection of ionospheric perturbations in TEC to a precise altitude. As TEC is weighted by the maximum ionospheric density, the corresponding altitude (hmF2) is, generally, assumed as the perturbation detection alti...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-30476-9

    authors： Thomas D,Bagiya MS,Sunil PS,Rolland L,Sunil AS,Mikesell TD,Nayak S,Mangalampalli S,Ramesh DS

    更新日期：2018-08-14 00:00:00

  • Inflammation is a key contributor to ovarian cancer cell seeding.

    abstract：:The incidence of ovarian cancer dramatically increases in early menopause but the factors contributing to cancer onset are unclear. Most ovarian cancers originate in the fallopian tube with subsequent implantation of malignant cells into the ovary. However, the events and conditions that lead to cancer cell implantati...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-30261-8

    authors： Jia D,Nagaoka Y,Katsumata M,Orsulic S

    更新日期：2018-08-17 00:00:00

  • Harnessing the microbiomes of Brassica vegetables for health issues.

    abstract：:Plant health is strongly connected with plants´ microbiome. In case of raw-eaten plants, the microbiome can also affect human health. To study potential impacts on health issues of both hosts, the microbiome composition of seven different Brassica vegetables, originating from different food processing pathways, was an...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-17949-z

    authors： Wassermann B,Rybakova D,Müller C,Berg G

    更新日期：2017-12-15 00:00:00

  • Combination of the Manifold Dimensionality Reduction Methods with Least Squares Support vector machines for Classifying the Species of Sorghum Seeds.

    abstract：:This study was carried out for rapid and noninvasive determination of the class of sorghum species by using the manifold dimensionality reduction (MDR) method and the nonlinear regression method of least squares support vector machines (LS-SVM) combing with the mid-infrared spectroscopy (MIRS) techniques. The methods ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep19917

    authors： Chen YM,Lin P,He JQ,He Y,Li XL

    更新日期：2016-01-28 00:00:00

  • Effect of size and charge asymmetry on aggregation kinetics of oppositely charged nanoparticles.

    abstract：:We report a theoretical and experimental study of the aggregation kinetics of oppositely charged nanoparticles. Kinetic Monte Carlo simulations are performed for symmetric, charge-asymmetric and size-asymmetric systems of oppositely charged nanoparticles. Simulation results show that both the weight and number average...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-40379-y

    authors： Singh K,Raghav A,Jha PK,Satapathi S

    更新日期：2019-03-06 00:00:00

  • Spatial Quantitation of Drugs in tissues using Liquid Extraction Surface Analysis Mass Spectrometry Imaging.

    abstract：:Liquid extraction surface analysis mass spectrometry imaging (LESA-MSI) has been shown to be an effective tissue profiling and imaging technique, producing robust and reliable qualitative distribution images of an analyte or analytes in tissue sections. Here, we expand the use of LESA-MSI beyond qualitative analysis t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep37648

    authors： Swales JG,Strittmatter N,Tucker JW,Clench MR,Webborn PJH,Goodwin RJA

    更新日期：2016-11-24 00:00:00

  • Naturally occurring proteinaceous nanoparticles in Coptidis Rhizoma extract act as concentration-dependent carriers that facilitate berberine absorption.

    abstract：:Pharmacological activities of some natural products diminish and even disappear after purification. In this study, we explored the mechanisms underlying the decrease of acute oral toxicity of Coptidis Rhizoma extract after purification. The water solubility, in vitro absorption, and plasma exposure of berberine (the m...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep20110

    authors： Ma BL,Yin C,Zhang BK,Dai Y,Jia YQ,Yang Y,Li Q,Shi R,Wang TM,Wu JS,Li YY,Lin G,Ma YM

    更新日期：2016-01-29 00:00:00

  • Design methodology for a confocal imaging system using an objective microlens array with an increased working distance.

    abstract：:In this study, a design methodology for a multi-optical probe confocal imaging system was developed. To develop an imaging system that has the required resolving power and imaging area, this study focused on a design methodology to create a scalable and easy-to-implement confocal imaging system. This system overcomes ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep33278

    authors： Choi W,Shin R,Lim J,Kang S

    更新日期：2016-09-12 00:00:00

  • Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci.

    abstract：:Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages to increase the effectivity of phage preparations against emerging phage-resistant strains. Here we described the genomic diversity of spontaneous host-range mutants of kayvirus 812. Five m...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-41868-w

    authors： Botka T,Pantůček R,Mašlaňová I,Benešík M,Petráš P,Růžičková V,Havlíčková P,Varga M,Žemličková H,Koláčková I,Florianová M,Jakubů V,Karpíšková R,Doškař J

    更新日期：2019-04-02 00:00:00

  • Efficient NH3-based process to remove chlorine from electron beam deposited ruthenium produced from (η3-C3H5)Ru(CO)3Cl.

    abstract：:The fabrication of Ru nanostructures by focused electron beam induced deposition (FEBID) requires suitable precursor molecules and processes to obtain the pure metal. So far this is problematic because established organometallic Ru precursors contain large organic ligands, such as cyclopentadienyl anions, that tend to...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-67803-y

    authors： Rohdenburg M,Boeckers H,Brewer CR,McElwee-White L,Swiderek P

    更新日期：2020-07-02 00:00:00

  • Genetic Variants Associated with Chronic Kidney Disease in a Spanish Population.

    abstract：:Chronic kidney disease (CKD) patients have many affected physiological pathways. Variations in the genes regulating these pathways might affect the incidence and predisposition to this disease. A total of 722 Spanish adults, including 548 patients and 174 controls, were genotyped to better understand the effects of ge...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-56695-2

    authors： Corredor Z,Filho MIDS,Rodríguez-Ribera L,Velázquez A,Hernández A,Catalano C,Hemminki K,Coll E,Silva I,Diaz JM,Ballarin J,Vallés Prats M,Calabia Martínez J,Försti A,Marcos R,Pastor S

    更新日期：2020-01-10 00:00:00

  • Colorectal Cancer Screening Programme in Spain: Results of Key Performance Indicators After Five Rounds (2000-2012).

    abstract：:Effective quality assurance is essential in any screening programme. This article provides a unique insight into key quality indicators of five rounds of the first population-based colorectal cancer screening programme implemented in Spain (2000-2012), providing the results according to the type of screening (prevalen...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep19532

    authors： Binefa G,Garcia M,Milà N,Fernández E,Rodríguez-Moranta F,Gonzalo N,Benito L,Clopés A,Guardiola J,Moreno V

    更新日期：2016-01-20 00:00:00

  • Synthetic Whole-Cell Biodevices for Targeted Degradation of Antibiotics.

    abstract：:Synthetic biology enables infinite possibilities in biotechnology via employing genetic modules. However, not many researches have explored the potentials of synthetic biology in environmental bioprocesses. In this study, we introduced a genetic module harboring the codon-optimized tetracycline degrading gene, tetX.co...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-21350-9

    authors： Xia PF,Li Q,Tan LR,Liu MM,Jin YS,Wang SG

    更新日期：2018-02-13 00:00:00

  • Para-aortic lymph node metastases in pancreatic cancer should not be considered a watershed for curative resection.

    abstract：:No international consensus regarding the resection of the para-aortic lymph node (PALN) station Ln16b1 during pancreatoduodenectomy for pancreatic ductal adenocarcinoma (PDAC) has been reached. The present retrospectively investigated 264 patients with PDAC who underwent curative pancreatoduodenectomy or total pancrea...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-08165-w

    authors： Hempel S,Plodeck V,Mierke F,Distler M,Aust DE,Saeger HD,Weitz J,Welsch T

    更新日期：2017-08-09 00:00:00

  • Hypofractionated stereotactic radiation therapy activates the peripheral immune response in operable stage I non-small-cell lung cancer.

    abstract：:It has been reported that in patients with operable stage I non-small cell lung cancer (NSCLC), overall survival (OS) is better in those who undergo hypofractionated stereotactic radiation therapy (HSRT) than in those who undergo surgery. However, the reason that HSRT has a better OS has not been fully explored. Here,...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-04978-x

    authors： Zhang T,Yu H,Ni C,Zhang T,Liu L,Lv Q,Zhang Z,Wang Z,Wu D,Wu P,Chen G,Wang L,Wei Q,Huang J,Wang X

    更新日期：2017-07-07 00:00:00