Abstract:
:The study examined (a) whether there is sex difference in spinal cord and plasma oxidative stress profiles in Dark Agouti rats immunised for experimental autoimmune encephalomyelitis (EAE), the principal experimental model of multiple sclerosis, and (b) whether there is correlation between the oxidative stress in spinal cord and neurological deficit. Regardless of rat sex, with the disease development xanthine oxidase (XO) activity and inducible nitric oxide synthase (iNOS) mRNA expression increased in spinal cord, whereas glutathione levels decreased. This was accompanied by the rise in spinal cord malondialdehyde level. On the other hand, with EAE development superoxide dismutase (SOD) activity decreased, while O2- concentration increased only in spinal cord of male rats. Consequently, SOD activity was lower, whereas O2- concentration was higher in spinal cord of male rats with clinically manifested EAE. XO activity and iNOS mRNA expression were also elevated in their spinal cord. Consistently, in the effector phase of EAE the concentration of advanced oxidation protein product (AOPP) was higher in spinal cord of male rats, which exhibit more severe neurological deficit than their female counterparts. In as much as data obtained in the experimental models could be translated to humans, the findings may be relevant for designing sex-specific antioxidant therapeutic strategies. Furthermore, the study indicated that the increased pro-oxidant-antioxidant balance in plasma may be an early indicator of EAE development. Moreover, it showed that plasma AOPP level may indicate not only actual activity of the disease, but also serve to predict severity of its course.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Dimitrijević M,Kotur-Stevuljević J,Stojić-Vukanić Z,Vujnović I,Pilipović I,Nacka-Aleksić M,Leposavić Gdoi
10.1007/s11064-016-2094-7subject
Has Abstractpub_date
2017-02-01 00:00:00pages
481-492issue
2eissn
0364-3190issn
1573-6903pii
10.1007/s11064-016-2094-7journal_volume
42pub_type
杂志文章abstract::Serotonin (5HT) synthesis in brain is influenced by precursor (tryptophan (TRP)) concentrations, which are modified by food ingestion. Hence, in rats, a carbohydrate meal raises brain TRP and 5HT; a protein-containing meal does not, but little attention has focused on differences among dietary proteins. Recently, sing...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0382-1
更新日期:2011-03-01 00:00:00
abstract::Activation of cholinergic muscarinic receptors results in an increased turnover of membrane inositol phospholipids. In rat cerebral cortex slices, carbachol- and acetylcholine-induced inositol phosphates ([3H]InsPs) accumulation is maximal in 7 day-old rats and lowest in adults, while the density of muscarinic binding...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969181
更新日期:1990-01-01 00:00:00
abstract::Role of the orexinergic system in pain modulation is well studied and involvement of the spinal orexin-1 receptors is well documented. In this study, we examined role of the spinal orexin-2 receptors in modulation of inflammatory pain in rat. Fifty-one adult male Wistar rats were implanted unilaterally with a guide ca...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-019-02749-w
更新日期:2019-05-01 00:00:00
abstract::A hemagglutinating activity was detected in a synaptic vesicle-enriched fraction prepared from adult rat brain, using trypsinized glutaraldehyde-fixed rabbit erythrocytes. The specific activity of the fraction, in two series of experiments, was 7.5 and 16-fold higher than in the other subcellular fractions. The activi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971856
更新日期:1988-01-01 00:00:00
abstract::It is an honour to have this opportunity write an article in recognition of the immense contributions of Bruce Ransom to the field of glial research. For me (BAM) personally there are many highlights both as a colleague and a friend that come to mind when I reflect on the many years that I have known Bruce. My own ent...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-017-2315-8
更新日期:2017-09-01 00:00:00
abstract::The kinetics and specificity of taurine and beta-alanine uptake were studied in primary cultures of rat astrocytes under identical experimental conditions. The uptake consisted of nonsaturable penetration and saturable high-affinity transport that was strictly sodium dependent. The cells accumulated taurine more effec...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967969
更新日期:1986-02-01 00:00:00
abstract::Hypoxia-induced oxidative stress is one of the major hallmark reasons underlying brain dysfunction. In the present manuscript, we have used CoCl2-induced hypoxic mice to investigate alterations in the activities of chief antioxidative stress enzymes- superoxide dismutase (SOD) and catalase (CAT) and expression of thei...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1388-x
更新日期:2014-09-01 00:00:00
abstract::(E)-2-(1-hydroxyl-4-oxocyclohexyl) ethyl caffeine (HOEC), a naturally caffeic ester isolated from Incarvillea mairei, has been reported to possess anti-inflammatory activity by targeting 5-lipoxygenase. However, its other potential activities have yet to be explored. In this study, we measured antioxidant activity of ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1498-5
更新日期:2015-03-01 00:00:00
abstract::Myelinating cells of both the peripheral and central nervous systems (CNSs) undergo dramatic cytoskeletal reorganization in order to differentiate and produce myelin. Myelinating oligodendrocytes in the CNS show a periodic actin pattern, demonstrating tight regulation of actin. Furthermore, recent data demonstrate tha...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-019-02753-0
更新日期:2020-03-01 00:00:00
abstract::The role of polyamines (PA) synthesis in NMDA receptor-mediated 45Ca2+ fluxes and norepinephrine release was studied in rat hippocampal synaptosomes. NMDA (50 microM) caused a sharp (> 2-fold) transient increase in PA synthesis regulating enzyme, ornithine decarboxylase (ODC) activity with concomitant elevation in PA ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00972471
更新日期:1994-11-01 00:00:00
abstract::In this study, we were interested to compare the responsiveness to growth factors, NGF, b-FGF and EGF and cytokines, IL1 beta, and TNF-alpha, in late passages (74-79) C6 glial cells committed astrocytes and astrocytes of advanced passages (26-28) in cultures derived from aged mouse cerebral hemispheres (MACH). Culture...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02527760
更新日期:1996-05-01 00:00:00
abstract::Hyperprolinemia type II (HPII) is an autosomal recessive disorder caused by the severe deficiency of enzyme delta1-pyrroline-5-carboxylic acid dehydrogenase leading to tissue accumulation of proline. Chronic administration of Pro led to significant reduction of cytosolic ALT activity of olfactory lobes (50.57%), cereb...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-004-7026-2
更新日期:2004-12-01 00:00:00
abstract::The first mentioning of the word "receptor" for the structure with which a bioactive compound should react for obtaining its specific influence on a physiological system goes back to the years around 1900. The receptor concept was adapted from the lock and key theory for the enzyme substrate and blockers interactions....
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-014-1398-8
更新日期:2014-10-01 00:00:00
abstract::Effects of methylmercury (MetHg) on the specific [3H]flunitrazepam binding were studied in rat cortical and cerebellar P2-fractions in vitro. MetHg did not affect significantly the specific [3H]flunitrazepam binding in unwashed P2-fraction but increased it marginally (by 16%) at 100 microM in washed P2-fraction, in bo...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01705532
更新日期:1995-06-01 00:00:00
abstract::The 12/15-lipoxygenase(s) (LOX), poly(ADP-ribose) polymerase (PARP-1) activity and mitochondrial apoptosis inducing factor (AIF) protein in the amyloid β (Aβ) toxicity were investigated in PC12 cells that express either wild-type (APPwt) or double Swedish mutation (APPsw) forms of human Aβ precursor protein. Different...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0412-7
更新日期:2011-05-01 00:00:00
abstract::We have adopted the PC12 cell line as in vitro cell model for studying Dp71 function in neuronal cells. These cells express a cytoplasmic (Dp71f) and a nuclear (Dp71d) isoform of Dp71 as well as various dystrophin-associated proteins (DAPs). In this study, we revealed by confocal microscopy analysis and Western blotti...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-0064-z
更新日期:2010-03-01 00:00:00
abstract::The folate cycle, which has a close correlation with nucleic acid synthesis as well as with sulfur amino acid metabolism, may have some bearing on the pathogenesis of amyotrophic lateral sclerosis. The folate cycle hypothesis offers a unified explanation of the reduction of RNA and the elevation of taurine in nervous ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00963985
更新日期:1984-03-01 00:00:00
abstract::Gangliosides, the acidic glycosphingolipids (GSLs) containing N-acetylgalactosamine and sialic acid are ubiquitous in the central nervous system. At least six DSL-glycosyltransferase activities (GLTs Gangliosides, the acidic glycosphingolipids (GSLs) containing N-acetylgalactosamine and sialic acid (or NAc-Neuraminic ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0762-9
更新日期:2012-06-01 00:00:00
abstract::Taurine is a semiessential amino acid that is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, and leukocytes. Taurine reaches up to 50 mM concentration in leukocytes. Taurine has been shown to be tissue-protecti...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/b:nere.0000010440.37629.17
更新日期:2004-01-01 00:00:00
abstract::We have previously showed that peptide neurotensin inhibits neuronal Na(+), K(+)-ATPase activity, an effect which involves high affinity neurotensin receptor. Nitric oxide (NO) acts as a neurotransmitter or as a neuromodulator when it is synthesized by neuronal nitric oxide synthase. Neurotensin effect on Na(+), K(+)-...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0552-9
更新日期:2011-12-01 00:00:00
abstract::The subcellular distribution of Ca2+/calmodulin-stimulated adenylate cyclase activity was studied in comparison with that of guanine nucleotide-stimulated cyclase activity. The distributions of these activities were similar among the crude fractions but differed among the purified subsynaptosomal fractions. The specif...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965018
更新日期:1986-03-01 00:00:00
abstract::Neonatal hypoxia/ischemia (HI) is the most common cause of developmental neurological, cognitive and behavioral deficits in children, with hyperoxia (HHI) treatment being a clinical therapy for newborn resuscitation. Although cerebral edema is a common outcome after HI, the mechanisms leading to excessive fluid accumu...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0308-y
更新日期:2010-12-01 00:00:00
abstract::Lewy bodies and Lewy neurites in the brain constitute the main histopathological features of Parkinson's disease (PD) and dementia with Lewy bodies. They comprise amyloid-like fibrils composed of α-synuclein (αS), a small protein (~14 kDa). Because the aggregation of αS in the brain has been implicated as a critical s...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-017-2382-x
更新日期:2017-12-01 00:00:00
abstract::Synaptosomal membrane proteins solubilized with 8% CHAPS-8 M urea were analyzed with two-dimensional electrophoresis (2DE). The membrane proteins were resolved up to 250 spots on a 2DE map, ranging in isoelectric points (pI) from 3.5 to 10.0 and molecular weights (MW) from 10 kDa to 200 kDa. Comparison of the mapped p...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966203
更新日期:1990-05-01 00:00:00
abstract::Triptolide (T10), an extract from the traditional Chinese herb, Tripterygium wilfordii Hook F (TWHF), has been shown to attenuate the rotational behavior induced by D: -amphetamine and prevent the loss of dopaminergic neurons in the substantia nigra in rat models of Parkinson's disease. To examine if the neuroprotecti...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9253-1
更新日期:2007-07-01 00:00:00
abstract::In order to develop a simplified method for long-term primary culture of highly-pure rat embryonic hippocampal neurons of low-density (10(3) cells/cm(2)), we optimized and modified conventional culturing methods. The modifications of our simplified method include: (1) combinational application of two growth substrates...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0189-0
更新日期:2010-09-01 00:00:00
abstract::Probenecid inhibits the transport of the small monocarboxylic acids lactate and propionate from blood to brain but does not affect the transport of 5HIAA or HVA. Neither lactate, 5HIAA, HVA, nor probenecid itself inhibits probenecid uptake into brain from blood and neither lactate nor 5HIAA itself inhibits 5HIAA uptak...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965898
更新日期:1982-10-01 00:00:00
abstract::Using the replication-defective adenovirus vector, we overexpressed rat glia maturation factor (GMF) in primary astrocyte cultures derived from embryonic rat brains. Among the three isoforms of MAP kinase, there was a big increase in the phosphorylation of p38, as detected with Western blotting using the phosphospecif...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1014241300179
更新日期:2001-12-01 00:00:00
abstract::Endoplasmic reticulum (ER) and mitochondria are intracellular organelles and their interactions are directly involved in different processes such as Ca(2+) signaling in cell survival and death mechanisms. Bcl-2 is an anti-apoptotic protein intrinsically related to ER and mitochondria, modulating Ca(2+) content in thes...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0600-5
更新日期:2012-02-01 00:00:00
abstract::Implication of protein phosphatases in Alzheimer disease led us to a systemic investigation of the identification of these enzyme activities in human brain. Human brain phosphatases eluted from DEAE-Sephacel with 0.22 M NaCl were resolved into two main groups by affi-gel blue chromatography, namely affi-gel blue-bindi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1007547701518
更新日期:2000-01-01 00:00:00