Abstract:
:Dermatophytoses include a wide variety of diseases involving glabrous skin, nails and hair. These superficial infections are a common cause of consultation in dermatology. In many cases, their diagnosis is not clinically obvious, and mycological analysis therefore is required. Direct microscopic examination of the samples using clearing agents provides a quick response to the clinician and is usually combined with cultures on specific media, which must be used to overcome the growth of contaminating moulds that may hamper the recovery of dermatophytes. Accurate identification of the causative agent (i.e. at the species level), currently based on morphological criteria, is necessary not only to initiate an appropriate treatment but also for setting prophylactic measures. However, conventional methods often lack sensitivity and species identification may require up to 4 weeks if subcultures are needed. Histological analysis, which is considered the "gold standard" for the diagnosis of onychomycoses, is seldom performed, and as direct examination, it does not allow precise identification of the pathogen. Nevertheless, a particular attention to the quality of clinical specimens is warranted. Moreover, the sensitivity of direct examination may be greatly enhanced by the use of fluorochromes such as calcofluor white. Likewise, sensitivity of the cultures could be enhanced by the use of culture media containing antifungal deactivators. With the generalization of molecular identification by gene sequencing or MALDI-TOF mass spectrometry, the contribution of historical biochemical or physiological tests to species identification of atypical isolates is now limited. Nevertheless, despite the recent availability of several PCR-based kits and an extensive literature on molecular methods allowing the detection of fungal DNA or both detection and direct identification of the main dermatophyte species, the biological diagnosis of dermatophytosis in 2016 still relies on both direct examination and cultures of appropriate clinical specimens.
journal_name
Mycopathologiajournal_title
Mycopathologiaauthors
Pihet M,Le Govic Ydoi
10.1007/s11046-016-0071-ysubject
Has Abstractpub_date
2017-02-01 00:00:00pages
169-180issue
1-2eissn
0301-486Xissn
1573-0832pii
10.1007/s11046-016-0071-yjournal_volume
182pub_type
杂志文章,评审相关文献
MYCOPATHOLOGIA文献大全abstract::We report a case of a 40-year-old Caucasian woman who came under our observation with a 7-year history of a chronic erythematous scaly dermatitis, diagnosed as psoriasis, involving gluteal area and thighs, and treated with topical steroids without benefit. During pregnancy, a progressive worsening of her condition and...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-011-9492-9
更新日期:2012-03-01 00:00:00
abstract::Candida yeasts are saprophytes naturally present in the environment and forming colonies on human mucous membranes and skin. They are opportunistic fungi that cause severe and even fatal infections in immunocompromised individuals. Several essential oils, including eucalyptus, pine, cinnamon and lemon, have been shown...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-013-9723-3
更新日期:2014-02-01 00:00:00
abstract::Twenty isolates of Verticillium fungicola var. fungicola collected from diseased fruit-bodies of Agaricus bisporus from prochloraz-treated crops, were exposed to a range of concentrations of six chemicals (benomyl, chlorothalonil, formaldehyde, iprodione, prochloraz-Mn-complex and prochloraz + carbendazim) in vitro. E...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00438918
更新日期:1996-12-01 00:00:00
abstract::Basidiospores were isolated from the fruiting bodies of Ganoderma infecting oil palms from an estate in Johor and from ornamental palms (including oil palms) from Singapore. The spores were then germinated to obtain homokaryotic mycelia. Based on clamp connection formation in paired hyphal fusions, tester strains were...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-004-4440-6
更新日期:2005-01-01 00:00:00
abstract::To clarify the terbinafine (TRF) resistance mechanism in a TRF-resistant strain of Microsporum canis, the expression of the pleiotropic drug resistance (PDR1), multidrug resistance (MDR1), MDR2 and MDR4 genes were investigated by real-time quantitative PCR (RT-qPCR) analysis, given the known interaction of the corresp...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-018-0242-0
更新日期:2018-06-01 00:00:00
abstract::A total of 169 strains of the Aspergillus reference cultures in the Aspergillus flavus group maintained in the American Type Culture Collection (ATCC) were studied for their aflatoxin-producing abilities in rice, peanut and semisynthetic medium, utilizing high pressure liquid chromatography. Fifty-nine of the strains ...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00437010
更新日期:1986-01-01 00:00:00
abstract::Growth of the biocontrol fungus Epicoccum nigrum was more sensitive to ionic solute water stress (NaCl) than non-ionic (glycerol) on potato dextrose-based media at -0.5, -3.0 and -5.5 MPa water potentials. Subsequent physiological manipulation of growth of E. nigrum in glycerol-modified media to -3.0 MPa water potenti...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1023/a:1022991025675
更新日期:2003-01-01 00:00:00
abstract::Ecologic and hygienic interests led us to analyze the mycoflora of the sands of a sand box for children's play in Turin. Along with traditional methods, we have also employed selective techniques suitable for the isolation of cellulolytic and keratinolytic fungi. A total of 89 species were isolated. As supported by th...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00437178
更新日期:1982-10-22 00:00:00
abstract::The prominence of seafood in Japan motivates close monitoring of its seas and marine lives for potentially pathogenic fungi. During the treatments of the male Pacific white-sided dolphin (Lagenorhynchus obliquidens) for paracoccidioidomycosis ceti (PCM-C), 5 white and floccose colonies showing identical genotype and m...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-020-00484-3
更新日期:2020-12-01 00:00:00
abstract::Onychomycosis are fungal infections affecting finger and toenails mainly caused by dermatophyte fungi and some Candida species. Low cure rates and frequent recurrence, development of a fungal resistance front to various antimicrobial agents topical and systemic, and an ineffective topical treatment make onychomycosis ...
journal_title:Mycopathologia
pub_type: 杂志文章,评审
doi:10.1007/s11046-015-9957-3
更新日期:2016-02-01 00:00:00
abstract::Candidiasis was experimentally produced in young Japanese quail by oral administration of Candida albicans cells. Lesions were confined to upper digestive tract with most characteristic changes occurring on the mucosa of crop. No lesions were observed in other tissues of the body. The initial changes in the crop were ...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF01103575
更新日期:1993-02-01 00:00:00
abstract::Four new species of Penicillium Link ex Fries are described and illustrated. All of them but one have been isolated from the atmosphere in Madrid. Spain. They clearly differ from all species of the genus described so far and are, therefore, described and proposed as new taxa: Penicillium aurantio-flammiferum sp. nov.,...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00443048
更新日期:1980-08-29 00:00:00
abstract::Recently 11 Malassezia species were isolated. Attention has focused on the relationship between Malassezia species and Malassezia-related disease. The causal fungus of Pityriasis versicolor is M. globosa. The conditions of mycelial form induction are not clear for M. globosa. ...
journal_title:Mycopathologia
pub_type: 杂志文章,评审
doi:10.1007/s11046-006-0081-2
更新日期:2006-12-01 00:00:00
abstract::A case of mycetoma caused by the black fungus Madurella grisea in northern Brazil is reported. The lesion was located on the patient's right foot without bone involvement. Clinical samples were collected by opening the fistules with a scalp and the grains removed for microbiological and histopathological analyses. Alt...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-004-2844-y
更新日期:2004-11-01 00:00:00
abstract::Three fungi, Alternaria humicola, A. pluriseptata and Aspergillus niger are being reported as new probable etiologic filamentous fungi, causing onychomycosis. The morphology and physiology of these fungi is discussed. ...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00437626
更新日期:1985-12-01 00:00:00
abstract::Two different kits for the detection of serum cryptococcal antigen in patients with pulmonary cryptococcosis were evaluated. The Eiken test (the Eiken Co., Tokyo), which uses pronase for pretreatment of serum, was compared with the Crypto-LA test (International Biological Laboratories, Cranbury, NJ), which did not use...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF01365083
更新日期:1993-08-01 00:00:00
abstract::The effect of a sub-inhibitory concentration of chlorhexidine on lipid and sterol composition of Candida albicans was investigated. The total lipid content of this yeast grown in the presence of chlorhexidine was reduced whilst the total sterol content was increased compared with control-grown cells. Lipids and sterol...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1023/a:1006852207366
更新日期:1997-01-01 00:00:00
abstract::We investigated phagocytosis and intracellular killing of clinical and environmental isolates of Aspergillus spp. by human monocyte-derived macrophages (MDMs). Serial pathogens such as Aspergillus fumigatus, Aspergillus flavus and Aspergillus terreus were examined with a microbiological assay. Phagocytosis for resting...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-007-9013-z
更新日期:2007-06-01 00:00:00
abstract::The ranunculaceous derivative protoanemonin (PrA) was studied as an antifungal agent on the dermatophyte Microsporum cookei. The ultrastructural changes that PrA brought about in this fungus were observed with both the transmission and scanning electron microscopes. The main anomalies noted were abnormally shaped hyph...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00436782
更新日期:1989-10-01 00:00:00
abstract::Candida albicans biofilms on most medical devices are exposed to a flow of body fluids that provide water and nutrients to the fungal cells. While C. albicans biofilms grown in vitro under static conditions have been exhaustively studied, the same is not true for biofilms developed under continuous flow of replenishin...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-009-9205-9
更新日期:2009-09-01 00:00:00
abstract::Kernel samples of common and dwarf Brazilian cashew nuts were highly contaminated with field and storage fungi in comparison to healthy ones. In general, dwarf cashews were more contaminated than common. A total of 37 fungal species were identified. Aspergillus niger was the dominant species with more colonies being i...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1023/a:1007081319685
更新日期:1999-01-01 00:00:00
abstract::Caries and chronic periodontitis are common oral diseases where a higher Candida colonization is reported. Antifungal agents could be adjuvant drugs for the therapy of both clinical conditions. The aim of the current study has been to evaluate the in vitro activities of conventional and new antifungal drugs against or...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-017-0112-1
更新日期:2017-06-01 00:00:00
abstract::The purpose of the present paper is to improve the understanding of the incidence of Candidosis in human infections. 85 species of Candida isolated from deep-seated and superficial Candidosis were studied. Identification was attained using the key of N. van Uden & H. Buckley (22). ...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00431478
更新日期:1985-02-01 00:00:00
abstract::Mucormycosis is an uncommon systemic mycosis affecting the immunocompromised individuals. It is usually caused by organisms of the genera Rhizopus and Mucor, although rarely other organisms have also been implicated. Mycoses due to these angioinvasive fungi have an acute onset, rapidly progressive course with high mor...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-014-9752-6
更新日期:2014-08-01 00:00:00
abstract::Scedosporium and Lomentospora species are the second most frequent colonizing, allergenic, or invasive fungal pathogens in patients with cystic fibrosis, and are responsible for infections varying from cutaneous and subcutaneous tissue infections caused by traumatic inoculation to severe systemic diseases in immunocom...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-017-0195-8
更新日期:2017-12-01 00:00:00
abstract::In a preliminary study, 256 feed samples collected from different parts of Northern India were examined for the presence of aflatoxigenic strains of Aspergillus favus/parasiticus and for detection of Aflatoxin B1 (AFB1). Out of 198 A. flavus and 15 A. parasiticus strains isolated, 76% and 86% respectively, were found ...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1023/a:1010960402254
更新日期:2001-01-01 00:00:00
abstract::Paracoccidioidomycosis (PCM) in children is rare, and its clinical progression is not clearly understood. The admission and post-admission clinical records of 38 children and teenagers aged up to 14 were studied who had been under treatment for 24-30 months. It is a consecutive case series, 17 retrospective and 21 pro...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-005-3653-7
更新日期:2006-02-01 00:00:00
abstract::In order to improve the isolation and identification of yeasts in a cancer research hospital, a protocol was developed utilizing an improved blood culture methodology and a four-test schema for rapid yeast identification. The blood culturing technique, based upon centrifugation, has shown a ten-fold increase in isolat...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00447183
更新日期:1978-12-18 00:00:00
abstract::A case is presented in which yeastlike cells resembling the yeast phase of Paracoccidioides brasiliensis were observed by microscopic examination of a urine sample. A mold identified as Mucor circinelloides was isolated from the specimen. It was converted to the yeast form by cultivation on Sabouraud agar incubated in...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00437243
更新日期:1987-03-01 00:00:00
abstract::Thirty-four isolates of the eight most common Fusarium species isolated from Norwegian cereals; F. avenaceum, F. culmorum, F. equiseti, F. graminearum, F. poae, F. sporotrichioides, F. torulosum and F. tricinctum were studied for their cytotoxicity and ability to produce mycotoxins. The strains were cultivated on rice...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1023/a:1007016820879
更新日期:1998-01-01 00:00:00