Abstract:
:Aminopeptidases have emerged as new promising drug targets for the development of novel anti-parasitic drugs. An aspartyl aminopeptidase-like gene has been identified in the Toxoplasma gondii genome (TgAAP), although its function remains unknown. In this study, we characterized TgAAP and performed functional analysis of the gene product. Firstly, we expressed a functional recombinant TgAAP (rTgAAP) protein in Escherichia coli, and found that it required metal ions for activity and showed a substrate preference for N-terminal acidic amino acids Glu and Asp. Then, we evaluated the function and drug target potential of TgAAP using the CRISPR/Cas9 knockout system. Western blotting demonstrated the deletion of TgAAP in the knockout strain. Indirect immunofluorescence analysis showed that TgAAP was localized in the cytoplasm of the wild-type parasite, but was not expressed in the knockout strain. Phenotype analysis revealed that TgAAP knockout inhibited the attachment/invasion, replication, and substrate-specific activity in T. gondii. Finally, the activity of drug CID 23724194, previously described as targeting Plasmodium and malarial parasite AAP, was tested against rTgAAP and the parasite. Overall, TgAAP knockout affected the growth of T. gondii but did not completely abolish parasite replication and growth. Therefore, TgAAP may comprise a useful adjunct drug target of T. gondii.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Zheng J,Cheng Z,Jia H,Zheng Ydoi
10.1038/srep34448subject
Has Abstractpub_date
2016-09-28 00:00:00pages
34448issn
2045-2322pii
srep34448journal_volume
6pub_type
杂志文章abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-25896-6
更新日期:2018-05-15 00:00:00
abstract::Brain insulin sensitivity is an important link between metabolism and cognitive dysfunction. Intranasal insulin is a promising tool to investigate central insulin action in humans. We evaluated the acute effects of 160 U intranasal insulin on resting-state brain functional connectivity in healthy young adults. Twenty-...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-017-01907-w
更新日期:2017-05-09 00:00:00
abstract::Similar to sleeping after learning, a brief period of wakeful resting after encoding new information supports memory retention in contrast to task-related cognition. Recent evidence suggests that working memory capacity (WMC) is related to sleep-dependent declarative memory consolidation. We tested whether WMC moderat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74760-z
更新日期:2020-10-21 00:00:00
abstract::Nile tilapia (Oreochromis niloticus) is a globally significant aquaculture species rapidly gaining status as a farmed commodity. In West Africa, wild Nile tilapia genetic resources are abundant yet knowledge of fine-scale population structure and patterns of natural genetic variation are limited. Coinciding with this ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53295-y
更新日期:2019-11-14 00:00:00
abstract::Dust from grain and feed production may cause adverse health effects in exposed workers. In this study we explored circulating miRNAs as potential biomarkers of occupational grain dust exposure. Twenty-two serum miRNAs were analyzed in 44 grain dust exposed workers and 22 controls. Exposed workers had significantly up...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68296-5
更新日期:2020-07-09 00:00:00
abstract::The functions of female song found in a few cicadas have rarely been studied. In the cicada Subpsaltria yangi we investigated the acoustic behaviour and signal structure of songs produced by females, the phonotaxis of males, and mate choice, as well as the selective pressure imposed on this species by predators. Pair-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06825-5
更新日期:2017-07-25 00:00:00
abstract::The dependences of spreading and differentiation of stem cells plated on hydrogel and silicone gel substrates on the rigidity and porosity of the substrates have recently been a subject of some controversy. In experiments on human mesenchymal stem cells plated on soft, medium rigidity, and hard silicone gels we show t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33411
更新日期:2016-09-21 00:00:00
abstract::Adiponectin is an adipokine that regulates apoptosis, glucose and lipid metabolism, and insulin sensitivity in metabolic diseases. As recent studies have associated changes in adipokines and other metabolites in the central nervous system with a risk for Alzheimer's disease (AD), we investigated the effects of adipone...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45509-0
更新日期:2019-06-20 00:00:00
abstract::The Tcf/Lef family of transcription factors mediates the Wnt/β-catenin pathway that is involved in a wide range of biological processes, including vertebrate embryogenesis and diverse pathogenesis. Post-translational modifications, including phosphorylation, sumoylation and acetylation, are known to be important for t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42590
更新日期:2017-02-15 00:00:00
abstract::The miniaturization of future electronic devices requires the knowledge of interfacial properties between two-dimensional channel materials and high-κ dielectrics in the limit of one atomic layer thickness. In this report, by combining particle-swarm optimization method with first-principles calculations, we present a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29221
更新日期:2016-07-06 00:00:00
abstract::Controlled ovarian stimulation by exogenous gonadotrophins is a key procedure during the in vitro fertilization cycle to obtain a sufficient number of oocytes in humans. Previous studies demonstrated that repeated superovulation had deleterious effects on the ovaries. However, whether repeated superovulation adversely...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31368
更新日期:2016-10-04 00:00:00
abstract::Grain boundary (GB) oxidation of proton-irradiated 304 nuclear grade stainless steel in primary water of pressurized water reactor was investigated. The investigation was conducted by studying microstructure of the oxide and oxide precursor formed at GB on an "atom-by-atom" basis by a combination of atom-probe tomogra...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80600-x
更新日期:2021-01-14 00:00:00
abstract::At the center of a collapsing hole lies a singularity, a point of infinite curvature where the governing equations break down. It is a topic of fundamental physical interest to clarify the dynamics of fluids approaching such singularities. Here, we use scaling arguments supported by high-fidelity simulations to analyz...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50956-w
更新日期:2019-10-10 00:00:00
abstract::Alcohol and nicotine are the most widely co-abused drugs. Both modify the activity of dopaminergic (DA) neurons of the Ventral Tegmental Area (VTA) and lead to an increase in DA release in the Nucleus Accumbens, thereby affecting the reward system. Evidences support the hypothesis that distinct nicotinic acetylcholine...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45116
更新日期:2017-03-23 00:00:00
abstract::Emergence of multiple drug resistant strains of pathogenic bacteria calls for new initiatives to combat infectious diseases. Gold nanoparticles (AuNPs), because of their non-toxic nature and size/shape dependent optical properties, offer interesting possibility. Here we report the antibacterial efficacy of AuNPs of di...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09357-0
更新日期:2017-08-29 00:00:00
abstract::Inspired by adhesive mussel proteins, nanospherical self-assemblies were prepared from bolaamphiphiles containing 3,4-dihydroxyphenylalanine (DOPA) moieties, and a suspension of the bolaamphiphile assemblies was used for the preparation of a patterned surface that enhanced cell adhesion and viability. The abundant sur...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14249-4
更新日期:2017-10-23 00:00:00
abstract::Myopia is the commonest eye disorder in the world. High myopes are predisposed to ocular pathologies. The vasoactive intestinal peptide receptor 2 (VIPR2) gene was identified as a myopia susceptibility locus by our group and another group. We continued to fine-map this locus. A case-control study was performed in 4 se...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54619-8
更新日期:2019-12-03 00:00:00
abstract::Receding angles have been shown to have great significance when designing a superhydrophobic surface for applications involving self-cleaning. Although apparent receding angles under dynamic conditions have been well studied, the microscopic receding contact line dynamics are not well understood. Therefore, experiment...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08384
更新日期:2015-02-11 00:00:00
abstract::Among abundant reconstructions of Holocene climate in Europe, only a handful has addressed winter conditions, and most of these are restricted in length and/or resolution. Here we present a record of late autumn through early winter air temperature and moisture source changes in East-Central Europe for the Holocene, b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01397-w
更新日期:2017-04-26 00:00:00
abstract::Melimine and Mel4 are chimeric cationic peptides with broad-spectrum antimicrobial activity. They have been shown to be highly biocompatible in animal models and human clinical trials. The current study examined the mechanism of action of these two antimicrobial peptides against P. aeruginosa. The effect of the peptid...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42440-2
更新日期:2019-05-08 00:00:00
abstract::Epithelial ovarian cancer (EOC) is the most deadly of the gynecological cancers. New approaches and better tools for monitoring treatment efficacy and disease progression of EOC are required. In this study, metabolomics using rapid resolution liquid chromatography mass spectrometry was applied to a systematic investig...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23334
更新日期:2016-03-21 00:00:00
abstract::It has been reported that non-selective autophagy of infected hepatocytes could facilitate the development of malaria in the liver stage, but the fate of parasites following selective autophagy of infected hepatocytes is still not very clear. Here, we confirmed that sporozoite infection can induce a selective autophag...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38170
更新日期:2016-11-30 00:00:00
abstract::There is growing interest in the conservation and utilization of crop wild relatives (CWR) in international food security policy and research. Legumes play an important role in human health, sustainable food production, global food security, and the resilience of current agricultural systems. Pea belongs to the ancien...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17623-4
更新日期:2017-12-12 00:00:00
abstract::We report here that CYB5D2 is associated with tumor suppression function in breast cancer (BC). CYB5D2 expression was significantly reduced in tamoxifen resistant MCF7 cells and in MCF7 cell-derived xenografts treated with TAM. CYB5D2 overexpression induced apoptosis in MCF7 cells; CYB5D2 knockdown enhanced MCF7 cell ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43006-y
更新日期:2019-04-29 00:00:00
abstract::Facio-Scapulo Humeral dystrophy (FSHD) is the third most common myopathy, affecting 1 amongst 10,000 individuals (FSHD1, OMIM #158900). This autosomal dominant pathology is associated in 95% of cases with genetic and epigenetic alterations in the subtelomeric region at the extremity of the long arm of chromosome 4 (q ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46861-x
更新日期:2019-07-17 00:00:00
abstract::Cell therapy using intramuscular injections of autologous bone-marrow mononuclear cells (BM-MNCs) improves clinical symptoms and can prevent limb amputation in atherosclerotic peripheral arterial disease (PAD) patients with critical limb ischemia (CLI). The purpose of this study was to evaluate the effects of the numb...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76886-6
更新日期:2020-11-16 00:00:00
abstract::Despite of the great scientific and technology interest, highly ordered full-Heusler L21-Co2MnAl films have remained a big challenge in terms of the availability and the electrical transport. Here we report the controllable growth and the intriguing transport behavior of epitaxial L21-Co2MnAl films, which exhibit a lo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42931
更新日期:2017-02-20 00:00:00
abstract::Bisphenol A (BPA), a member of the environmental endocrine disruptors (EDCs), has recently received increased attention because of its effects on brain insulin resistance. Available data have indicated that brain insulin resistance may contribute to neurodegenerative diseases. However, the associated mechanisms that u...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07544-7
更新日期:2017-08-08 00:00:00
abstract::Accurate and detailed knowledge of California's groundwater is of paramount importance for statewide water resources planning and management, and to sustain a multi-billion-dollar agriculture industry during prolonged droughts. In this study, we use water supply and demand information from California's Department of W...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31210-1
更新日期:2018-08-27 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-71638-y
更新日期:2020-09-09 00:00:00