Abstract：
:Lipids are emerging as key regulators of fundamental cellular processes including cell survival, division, and death. Apoptosis, a form of programmed cell death, is accompanied by numerous membrane-related phenotypic changes. However, we have an incomplete understanding of the involvement of specific lipid structures during this process. Here, we report that triacylglycerols are regulated at the molecular level during 5-fluorouracil-induced apoptosis in HCT-116. Mass-spectrometry-based global lipid profiling shows that specific triacylglycerols accumulate during apoptosis. Expression levels and activities of enzymes that are responsible for the biosynthesis and metabolic processing of triacylglycerols suggest that triacylglycerol biosynthesis is responsible for these accumulations. Based on our data, we propose that regulation of triacylglycerols at the molecular level happens downstream of p53 activation and potentially is a mechanism to prevent lipid oxidation during apoptosis.
journal_name
ACS Chem Bioljournal_title
ACS chemical biologyauthors
Li N,Lizardo DY,Atilla-Gokcumen GEdoi
10.1021/acschembio.6b00410subject
Has Abstractpub_date
2016-09-16 00:00:00pages
2583-7issue
9eissn
1554-8929issn
1554-8937journal_volume
11pub_type
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