Abstract:
AIMS:Sentinel lymph node (SLN) biopsy is the preferred surgical technique for staging the axilla in clinically node-negative breast cancer. Accurate intraoperative staging allows for the immediate performance of an axillary clearance in node-positive patients. We assessed the Metasin assay for the intraoperative analysis of SLNs in a prospective evaluation of 250 consecutive patients undergoing intraoperative SLN analysis at the Breast Unit, University Hospital, Southampton, UK. METHODS:Metasin uses a quantitative reverse transcription PCR to detect two markers of metastasis: cytokeratin 19 (CK19) an epithelial marker and mammaglobin (MGB) a breast specific marker. Metasin results were compared with the results from routine paraffin block histopathology. RESULTS:Metasin was robust, with a failure rate of <1%, and demonstrated excellent accuracy and reproducibility. The average turnaround time for the Metasin assay was 42 min, the largest variable being the number of nodes assayed. A total of 533 SLNs were evaluated with 75 patients testing positive for MGB and/or CK19. Based on the analysis of individual SLNs, the overall concordance between Metasin and histology was 92.3% (sensitivity 88.7%, specificity 92.9%). When adjusted for tissue allocation bias, the concordance was 93.8% (sensitivity 89.8%, specificity 94.6%). In this evaluation, 57/250 patients (23%) proceeded to axillary clearance based on Metasin results and were considered spared a second operative procedure. CONCLUSIONS:Metasin has proven to be an accurate, reproducible and reliable laboratory test. The analysis time is acceptable for intraoperative use, and in comparison to routine histology demonstrates acceptable concordance, sensitivity and specificity.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Smith GJ,Hodges E,Markham H,Zhang S,Cutress RIdoi
10.1136/jclinpath-2016-203728subject
Has Abstractpub_date
2017-02-01 00:00:00pages
134-139issue
2eissn
0021-9746issn
1472-4146pii
jclinpath-2016-203728journal_volume
70pub_type
杂志文章abstract:AIMS:The miR-9 family microRNAs (miRNAs) are tumour suppressor miRNAs implicated in carcinogenesis. We postulated that miR-9-1, miR-9-2 and miR-9-3 may be inactivated by aberrant promoter methylation in multiple myeloma (MM). METHODS:Methylation of miR-9-1, miR-9-2 and miR-9-3 was studied by methylation-specific PCR (...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202817
更新日期:2015-07-01 00:00:00
abstract::The calcium, vitamin D, and osteocalcin concentrations were investigated in 17 patients with anorexia nervosa. Serum 25-hydroxyvitamin D (25 OHD) concentrations below normal were observed in 15 (88%); only two patients has serum 1,25 dihydroxycholecalciferol (1,25(OH)2D) concentrations below normal. Serum parathyroid ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.2.195
更新日期:1988-02-01 00:00:00
abstract:AIMS:To investigate the association of cagA positivity and non-opsonic neutrophil activation capacity in wild-type Helicobacter pylori strains with peptic ulcer disease or chronic gastritis only. METHODS:Helicobacter pylori were isolated from antral biopsies of 53 consecutive patients with chronic antral gastritis, of...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.53.4.318
更新日期:2000-04-01 00:00:00
abstract:BACKGROUND:Prognostic factors in predicting outcomes in patients with head and neck squamous-cell carcinoma (HNSCC) are limited to the clinical-pathological parameters, including lymph node metastasis, location, grade and stage of the disease. AIM:To determine whether the expression of these proteins has a value in pr...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.036624
更新日期:2007-02-01 00:00:00
abstract:AIMS:To perform a clinicopathological analysis of a series of primary cutaneous Ewing sarcomas/primitive neuroectodermal tumours (ES/PNET) to highlight the pathological features, discuss the differential diagnosis, emphasise the role of molecular testing (particularly fluorescence in situ hybridisation, FISH) in diagno...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2008.064014
更新日期:2009-10-01 00:00:00
abstract::Distinctly increased levels of antibodies to measles virus envelope antigens haemolysin and haemagglutinin were found in the sera of patients with chronic active hepatitis compared with a normal control group, using immunofluorescence and functional tests. Similarly, a higher incidence of smooth muscle antibody of bot...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.11.1281
更新日期:1985-11-01 00:00:00
abstract:INTRODUCTION:The role of single mutations has been extensively studied myelodysplastic syndromes (MDS), but the impact of genetic aberrations in the context of other mutations is less well understood. BCOR is an epigenetic transcriptional corepressor. In MDS, BCOR mutations are rare and certain mutations are associated...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2019-206132
更新日期:2020-04-01 00:00:00
abstract::Methods of speciating Candida isolates from clinical specimens are described and the necessity of speciation is emphasized. Differences in susceptibility of C. albicans and C. tropicalis to amphotericin B were observed and the implications of this in relation to treatment with amphotericin B and the development of res...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.26.6.405
更新日期:1973-06-01 00:00:00
abstract::A case of chronic neutrophilic leukaemia (CNL) is reported. The diagnosis was based on leucocytosis with mature neutrophils, a raised leucocyte alkaline phosphatase score, negative Philadelphia chromosome, and extensive infiltration of neutrophils in various differentiation stages into the bone marrow. When viewed by ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.4.367
更新日期:1994-04-01 00:00:00
abstract:AIMS:Ki-67 is a prognostic marker in breast cancer; however, the use of the Ki-67 labelling index (LI) in clinical practice requires a consistent and easily accessible scoring method. The present study evaluated the use of the free internet-based image analysis program ImmunoRatio to score Ki-67 LI in breast cancer in ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-204162
更新日期:2017-08-01 00:00:00
abstract::A monoclonal antibody, produced by peptide immunisation was used to detect the distribution of p62c/myc by immunohistology in normal and coeliac small intestinal mucosa. The effect of gluten in four treated coeliac patients was investigated by taking serial jejunal biopsy specimens for six hours after a 10 g oral glut...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.3.307
更新日期:1987-03-01 00:00:00
abstract:AIMS:Hypomethylating agents (HMAs) exhibit clinical efficacy in patients with myelodysplastic syndromes (MDS) and myelodysplastic/myeloproliferative neoplasms (MDS/MPN). This study was performed to assess residual disease status by flow cytometry immunophenotyping (FCI) methods in patients with MDS or MDS/MPN treated w...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-203715
更新日期:2016-04-15 00:00:00
abstract::An immunological method for detecting fibrin degradation products by tanned cells agglutination has been standardized. The effects of different variables, such as cell concentration, pH, and length of incubation, and the specificity of the reaction, are described. The method is compared with other immunological techni...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.23.4.299
更新日期:1970-05-01 00:00:00
abstract::The Compu-pet 100 diluter/dispenser is a versatile and accurate instrument which can be employed for a wide variety of microbiological tests and techniques. Time in performing tests is often more than halved when the diluter is used. Large numbers of tests can be performed with minimal fatigue. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.1.37
更新日期:1975-01-01 00:00:00
abstract:BACKGROUND:Fatty liver disease (FLD), of either alcoholic (AFLD) or non-alcoholic (NAFLD) aetiologies, is characterised histologically by features that are lobulocentric that typically affect zone 3, in contrast with portal-based inflammation characteristics of other forms of chronic liver disease. AIM:The authors aim...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2010.079145
更新日期:2010-09-01 00:00:00
abstract::Splenic pathology was analysed in 73 patients with immune thrombocytopenic purpura who underwent splenectomy for bleeding that had been resistant to adrenocorticosteroids. The mean splenic weight was 100 g. The only notable macroscopic feature was the prominence of Malpighian corpuscles in 15 cases. Microscopic examin...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.9.985
更新日期:1985-09-01 00:00:00
abstract::A patient with rhabdomyosarcoma is described in whom the presenting clinical and laboratory features were those of disseminated intravascular coagulation. The patient's rapid downhill course was primarily expressed by haemorrhagic tendency. An alveolar rhabdomyosarcoma, affecting many organs, including vascular and ca...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.7.661
更新日期:1977-07-01 00:00:00
abstract:AIMS:To ascertain the effect of enhanced surveillance following an outbreak of waterborne cryptosporidiosis on the number of faecal specimens submitted to the local microbiology laboratory and the number positive for common enteric pathogens. The outbreak provided an opportunity to estimate the extent of routine under ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.55.4.271
更新日期:2002-04-01 00:00:00
abstract::Three strains of Past. septica were isolated in Hong Kong. The clinical histories and the detailed bacteriological and serological studies are presented. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.17.2.107
更新日期:1964-03-01 00:00:00
abstract::Renal involvement as the first manifestation of sarcoidosis is rare and has never been reported in India. This report describes a 35 year old man who was admitted to the emergency department with a clinical diagnosis of acute on chronic renal failure, secondary to obstructive uropathy. Postmortem examination unexpecte...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2004.018325
更新日期:2004-10-01 00:00:00
abstract::A 60 year old woman with disseminated ductal carcinoma of the breast developed non-islet cell tumour induced hypoglycaemia (NICTH). The concentrations of the two insulin-like growth factors, IGF-I and IGF-II, were < 2 nmol/l and 94.1 nmol/l, respectively. The IGF-II to IGF-I ratio was > 47 (normal < 10). Insulin (< 3 ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.10.780
更新日期:1999-10-01 00:00:00
abstract:AIMS:To evaluate two detailed step sectioning protocols for sentinel lymph nodes (SLNs). METHODS:After vital dye or combined dye and radiocolloid guided biopsy, SLNs were fixed in formalin and embedded in paraffin wax. In protocol A, SLNs from 123 patients were sectioned in steps of 50-100 micro m, whereas in protocol...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.55.12.926
更新日期:2002-12-01 00:00:00
abstract:AIM:To establish the diagnostic value of in situ hybridisation and the nested polymerase chain reaction (PCR) in detecting clinically relevant cytomegalovirus (CMV) infection in upper gastrointestinal biopsies from heart transplant patients. METHODS:Test sensitivity and specificity for detection of CMV early gene RNA ...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章
doi:10.1136/jcp.51.11.807
更新日期:1998-11-01 00:00:00
abstract:AIMS:The generation and characterisation of a monoclonal antibody that specifically recognises the mdr-1 encoded protein, P-glycoprotein (P-170), on routinely processed formalin fixed, paraffin wax embedded tissue sections. METHODS:The monoclonal antibody, designated 6/1C, was produced following a combination of in vi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.6.465
更新日期:1997-06-01 00:00:00
abstract::In the present investigation, the prevalence of focal lymphocytic adenitis in the submandibular salivary gland was observed in a series of 116 postmortem subjects after suitable exclusions had been made. Focal lymphocytic adenitis could not be demonstrated in the labial salivary glands. The degree of lymphocytic infil...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.23.8.690
更新日期:1970-11-01 00:00:00
abstract:AIMS:To characterise further the proliferative compartment of the normal cervix and to document its alteration, if any, in the various grades of cervical intraepithelial neoplasia (CIN), particularly changes to the basal epithelial layer; to hypothesise as to the diagnostic and biological significance of any observed d...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.8.667
更新日期:1996-08-01 00:00:00
abstract::The detection of lymphoid cells by routine examination of the urine after renal allotransplantation has proved to be a useful early indication of rejection. In a study of 36 rejection episodes, 20 (56%) were associated with a significant number of lymphocytes in the urine. The incidence was much higher when rejection ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.23.2.99
更新日期:1970-03-01 00:00:00
abstract::Platelet adhesiveness was measured in patients undergoing operation. With the rotating bulb technique adhesiveness increased steadily to the sixth and ninth days following surgery. The glass bead filter technique demonstrated a marked increase in platelet adhesiveness on the day following operation. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.20.5.708
更新日期:1967-09-01 00:00:00
abstract::Mesothelial lesions pose considerable diagnostic challenges not only because benign tumours, reactive proliferations and malignant mesothelioma can mimic one another, but also because the morphological patterns displayed by malignant mesothelioma can simulate a variety of epithelial and non-epithelial malignancies. Im...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.029652
更新日期:2006-06-01 00:00:00
abstract::Endogenous Cushing's syndrome is a rare endocrine disorder that incurs significant cardiovascular morbidity and mortality, due to glucocorticoid excess. It comprises adrenal (20%) and non-adrenal (80%) aetiologies. While the majority of cases are attributed to pituitary or ectopic corticotropin (ACTH) overproduction, ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2014-202612
更新日期:2015-03-01 00:00:00