Abstract:
:Microorganisms form biofilms containing differentiated cell populations. To determine factors driving differentiation, we herein visualize protein and metal distributions within Pseudomonas aeruginosa biofilms using imaging mass spectrometry. These in vitro experiments reveal correlations between differential protein distribution and metal abundance. Notably, zinc- and manganese-depleted portions of the biofilm repress the production of anti-staphylococcal molecules. Exposure to calprotectin (a host protein known to sequester metal ions at infectious foci) recapitulates responses occurring within metal-deplete portions of the biofilm and promotes interaction between P. aeruginosa and Staphylococcus aureus. Consistent with these results, the presence of calprotectin promotes co-colonization of the murine lung, and polymicrobial communities are found to co-exist in calprotectin-enriched airspaces of a cystic fibrosis lung explant. These findings, which demonstrate that metal fluctuations are a driving force of microbial community structure, have clinical implications because of the frequent occurrence of P. aeruginosa and S. aureus co-infections.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Wakeman CA,Moore JL,Noto MJ,Zhang Y,Singleton MD,Prentice BM,Gilston BA,Doster RS,Gaddy JA,Chazin WJ,Caprioli RM,Skaar EPdoi
10.1038/ncomms11951subject
Has Abstractpub_date
2016-06-15 00:00:00pages
11951issn
2041-1723pii
ncomms11951journal_volume
7pub_type
杂志文章abstract::Very-Low-Frequency (VLF) transmitters operate worldwide mostly at frequencies of 10-30 kilohertz for submarine communications. While it has been of intense scientific interest and practical importance to understand whether VLF transmitters can affect the natural environment of charged energetic particles, for decades ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18545-y
更新日期:2020-09-24 00:00:00
abstract::DNA methylation plays important roles in prokaryotes, and their genomic landscapes-prokaryotic epigenomes-have recently begun to be disclosed. However, our knowledge of prokaryotic methylation systems is focused on those of culturable microbes, which are rare in nature. Here, we used single-molecule real-time and circ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-08103-y
更新日期:2019-01-11 00:00:00
abstract::Electrospun nanofibres are promising in biomedical applications to replicate features of the natural extracellular matrix (ECM). However, nearly all electrospun scaffolds are either non-degradable or degrade hydrolytically, whereas natural ECM degrades proteolytically, often through matrix metalloproteinases. Here we ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7639
更新日期:2015-03-23 00:00:00
abstract::Experience-dependent plasticity of cell and tissue function is critical for survival by allowing organisms to dynamically adjust physiological processes in response to changing or harsh environmental conditions. Despite the conferred evolutionary advantage, it remains unknown whether emergent experience-dependent prop...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1612
更新日期:2012-01-03 00:00:00
abstract::The role of microglia in spinal cord injury (SCI) remains poorly understood and is often confused with the response of macrophages. Here, we use specific transgenic mouse lines and depleting agents to understand the response of microglia after SCI. We find that microglia are highly dynamic and proliferate extensively ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-08446-0
更新日期:2019-01-31 00:00:00
abstract::Cosmic rays interact with the Earth's atmosphere to produce 14C, which can be absorbed by trees. Therefore, rapid increases of 14C in tree rings can be used to probe previous cosmic-ray events. By this method, three 14C rapidly increasing events have been found. Plausible causes of these events include large solar pro...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01698-8
更新日期:2017-11-14 00:00:00
abstract::The hallmark of Salmonella Typhimurium infection is an acute intestinal inflammatory response, which is mediated through the action of secreted bacterial effector proteins. The pro-inflammatory Salmonella effector SopA is a HECT-like E3 ligase, which was previously proposed to activate host RING ligases TRIM56 and TRI...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14004
更新日期:2017-01-13 00:00:00
abstract::The properties of antihydrogen are expected to be identical to those of hydrogen, and any differences would constitute a profound challenge to the fundamental theories of physics. The most commonly discussed antiatom-based tests of these theories are searches for antihydrogen-hydrogen spectral differences (tests of CP...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4955
更新日期:2014-06-03 00:00:00
abstract::It is unclear whether the establishment of apical-basal cell polarity during the generation of epithelial lumens requires molecules acting at the plasma membrane/actin interface. Here, we show that the I-BAR-containing IRSp53 protein controls lumen formation and the positioning of the polarity determinants aPKC and po...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17091-x
更新日期:2020-07-14 00:00:00
abstract::Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via a two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localization of the receptors proposed the endoplasmic reticulum (ER) membrane as a principal cytokinin perception site,...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17949-0
更新日期:2020-08-27 00:00:00
abstract::The ability to detect and deal with errors when manipulating quantum systems is a fundamental requirement for fault-tolerant quantum computing. Unlike classical bits that are subject to only digital bit-flip errors, quantum bits are susceptible to a much larger spectrum of errors, for which any complete quantum error-...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7979
更新日期:2015-04-29 00:00:00
abstract::Microbial production of isoprene from renewable feedstock is a promising alternative to traditional petroleum-based processes. Currently, efforts to improve isoprenoid production in Saccharomyces cerevisiae mainly focus on cytoplasmic engineering, whereas comprehensive engineering of multiple subcellular compartments ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12851
更新日期:2016-09-21 00:00:00
abstract::Identification of the complete set of translated genes of viruses is important to understand viral replication and pathogenesis as well as for therapeutic approaches to control viral infection. Here, we use chemical proteomics, integrating bio-orthogonal non-canonical amino acid tagging and high-resolution mass spectr...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18718-9
更新日期:2020-09-29 00:00:00
abstract::Disordered proteins, such as those central to Alzheimer's and Parkinson's, are particularly intractable for structure-targeted therapeutic design. Here we demonstrate the capacity of a synthetic foldamer to capture structure in a disease relevant peptide. Oligoquinoline amides have a defined fold with a solvent-exclud...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11412
更新日期:2016-04-25 00:00:00
abstract::Protein aggregation plays a key role in neurodegenerative disease, giving rise to small oligomers that may become cytotoxic to cells. The fundamental microscopic reactions taking place during aggregation, and their rate constants, have been difficult to determine due to lack of suitable methods to identify and follow ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms8025
更新日期:2015-04-30 00:00:00
abstract::Genetic factors and mechanisms underlying food allergy are largely unknown. Due to heterogeneity of symptoms a reliable diagnosis is often difficult to make. Here, we report a genome-wide association study on food allergy diagnosed by oral food challenge in 497 cases and 2387 controls. We identify five loci at genome-...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01220-0
更新日期:2017-10-20 00:00:00
abstract::Radiotherapy is not currently informed by the genetic composition of an individual patient's tumour. To identify genetic features regulating survival after DNA damage, here we conduct large-scale profiling of cellular survival after exposure to radiation in a diverse collection of 533 genetically annotated human tumou...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11428
更新日期:2016-04-25 00:00:00
abstract::Studies on vesicle formation by the Coat Protein I (COPI) complex have contributed to a basic understanding of how vesicular transport is initiated. Phosphatidic acid (PA) and diacylglycerol (DAG) have been found previously to be required for the fission stage of COPI vesicle formation. Here, we find that PA with vary...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11324-4
更新日期:2019-07-30 00:00:00
abstract::Loss of protein and organelle quality control secondary to reduced autophagy is a hallmark of aging. However, the physiologic and molecular regulation of autophagy in long-lived organisms remains incompletely understood. Here we show that the Kruppel-like family of transcription factors are important regulators of aut...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-00899-5
更新日期:2017-10-13 00:00:00
abstract::An essential issue in the development of materials presenting an accurately functionalized surface is to achieve control of layer structuring. Whereas the very popular method based on the spontaneous adsorption of alkanethiols on metal faces stability problems, the reductive electrografting of aryldiazonium salts yiel...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2121
更新日期:2012-01-01 00:00:00
abstract::One of the important challenges in condensed matter science is to understand ultrafast, atomic-scale fluctuations that dictate dynamic processes in equilibrium and non-equilibrium materials. Here, we report an important step towards reaching that goal by using a state-of-the-art perfect crystal based split-and-delay s...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04178-9
更新日期:2018-04-27 00:00:00
abstract::Under hypoxia, most of glucose is converted to secretory lactate, which leads to the overuse of glutamine-carbon. However, under such a condition how glutamine nitrogen is disposed to avoid over-accumulating ammonia remains to be determined. Here we identify a metabolic flux of glutamine to secretory dihydroorotate, w...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-08033-9
更新日期:2019-01-14 00:00:00
abstract::As whole-genome sequencing for cancer genome analysis becomes a clinical tool, a full understanding of the variables affecting sequencing analysis output is required. Here using tumour-normal sample pairs from two different types of cancer, chronic lymphocytic leukaemia and medulloblastoma, we conduct a benchmarking e...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10001
更新日期:2015-12-09 00:00:00
abstract::It is vital to design effective nitrogen fixation systems that operate under mild conditions, and to this end we recently reported an example of the catalytic formation of ammonia using a dinitrogen-bridged dimolybdenum complex bearing a pincer ligand, where up to twenty three equivalents of ammonia were produced base...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4737
更新日期:2014-04-28 00:00:00
abstract::Identifying regions important for spreading and mediating perturbations is crucial to assess the susceptibilities of spatio-temporal complex systems such as the Earth's climate to volcanic eruptions, extreme events or geoengineering. Here a data-driven approach is introduced based on a dimension reduction, causal reco...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9502
更新日期:2015-10-07 00:00:00
abstract::Transgenerational epigenetic inheritance refers to the transmission of epigenetic information through the germline. While it has been observed in plants, nematodes and fruit flies, its occurrence in mammals-and humans in particular-is the matter of controversial debate, mostly because the study of transgenerational ep...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05445-5
更新日期:2018-07-30 00:00:00
abstract::The bacteria-derived clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems are powerful tools for genome engineering. Recently, in addition to Cas protein engineering, the improvement of guide RNAs are also performed, contributing to broadening the research area of CRISPR-Cas9 systems. Here we ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01650-w
更新日期:2017-11-23 00:00:00
abstract::Emerging quantum technologies require mastering thermal management, especially at the nanoscale. It is now accepted that thermal metamaterial-based phonon manipulation is possible, especially at sub-kelvin temperatures. In these extreme limits of low temperatures and dimensions, heat conduction enters a quantum regime...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06791-0
更新日期:2018-10-16 00:00:00
abstract::The Johnson-Kendall-Roberts theory is the basis of modern contact mechanics. It describes how two deformable objects adhere together, driven by adhesion energy and opposed by elasticity. Here we characterize the indentation of glass particles into soft, silicone substrates using confocal microscopy. We show that, wher...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3728
更新日期:2013-01-01 00:00:00
abstract::Reliable quantum information processing in the face of errors is a major fundamental and technological challenge. Quantum error correction protects quantum states by encoding a logical quantum bit (qubit) in multiple physical qubits. To be compatible with universal fault-tolerant computations, it is essential that sta...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11526
更新日期:2016-05-05 00:00:00