Abstract:
INTRODUCTION:Previously, we have shown that stem cells from apical papilla (SCAPs) can be chemoattracted by stromal cell-derived factor-1α (SDF-1α). The purpose of this study was to investigate the intracellular signaling pathways involved in SDF-1α-mediated migration of SCAPs. METHODS:Chemotaxis assays were performed to assess the effect of phosphatidylinositol 3-kinase (PI3K) and protein kinase C (PKC) signaling pathways in the SDF-1α-mediated migration of SCAPs using inhibitors of PI3K (LY294002) or PKC (GF109203X). The Cell Counting Kit-8 assay (Dojindo Laboratories, Kumamoto, Japan) was used to evaluate the effect of the inhibitors on the proliferation of SCAPs. The expression of focal adhesion-related proteins was examined by immunofluorescence staining and Western blot analysis. Phosphorylation of PI3K subunit p85 and PKC after SDF-1α induction was evaluated by Western blot. RESULTS:The inhibition of PI3K or PKC signaling pathways significantly reduced SDF-1α-mediated migration of SCAPs. The inhibitors had no effect on the proliferation of SCAPs. Immunofluorescence analysis revealed that SDF-1α stimulated focal adhesion formation and stress fiber assembly in SCAPs, in addition to up-regulation of the expression of focal adhesion molecules, including p-focal adhesion kinase, p-paxillin, and vinculin. Pretreatment with PI3K or PKC inhibitors before SDF-1α induction significantly inhibited focal adhesion molecule expression. Moreover, increased phosphorylation of p85 and PKC were observed after SDF-1α stimulation, whereas these phosphorylations were down-regulated by the inhibition of PI3K or PKC signaling pathways. CONCLUSIONS:PI3K and PKC signaling pathways appear to be required for SDF-1α-mediated transmigration of SCAPs. These findings provide insights into the signaling mechanisms that underlie SDF-1α-mediated migration of SCAPs.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Chen X,Liu JY,Yue L,Huang GT,Zou XYdoi
10.1016/j.joen.2016.04.014subject
Has Abstractpub_date
2016-07-01 00:00:00pages
1076-81issue
7eissn
0099-2399issn
1878-3554pii
S0099-2399(16)30193-5journal_volume
42pub_type
杂志文章abstract:INTRODUCTION:It has been widely accepted that dental pulp stem cells (DPSCs), which are a class of self-renewal and differentiation potential of adult stem cells, play an important role in the repair procession of pulp's inflammation. We investigated whether tumor necrosis factor alpha (TNF-α) could induce the prolifer...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.02.020
更新日期:2015-07-01 00:00:00
abstract:INTRODUCTION:Calcium hydroxide (Ca[OH]2) is a widely used interappointment dressing, but its antibacterial property is compromised by dentin. Hence, the addition of chlorhexidine (CHX) with Ca(OH)2 has been proposed. However, the antimicrobial efficacy of this mixture compared with Ca(OH)2 alone is currently still deba...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.01.020
更新日期:2016-05-01 00:00:00
abstract::There is an increasing interest in the utility of dental pulp stem cells (DPSCs) for dentin regeneration. The mechanisms involved in DPSC differentiation remain poorly understood. The purpose of the study was to investigate the mineralization capacity of human dental pulp cells (DPCs) and identify potential markers fo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.02.009
更新日期:2007-06-01 00:00:00
abstract::The aim of this study was to evaluate the presence of bacterial biofilm on the external surface of the root apex in teeth with pulp necrosis, with and without radiographically visible periapical lesions, and in teeth with a vital pulp. Twenty-one teeth were extracted, eight with pulp necrosis and periapical lesions, e...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200212000-00006
更新日期:2002-12-01 00:00:00
abstract::Because of its close proximity to teeth and periodontium, gingival squamous cell carcinoma (SCC) can sometimes mimic tooth-related benign inflammatory conditions, resulting in misdiagnosis. In this study we report a case of gingival SCC that mimicked a dentoalveolar abscess of endodontic origin in its early presentati...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.08.005
更新日期:2007-02-01 00:00:00
abstract::The purpose of this study was to compare Schick CDR and Trophy RVGui direct digital radiography (DDR) systems for the ability to detect periapical lesions in human cadaver mandibles. Digital radiographs were exposed of teeth with normal periapical areas and of teeth with artificially prepared periapical lesions using ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000140571.23012.ff
更新日期:2005-04-01 00:00:00
abstract::Pulp revascularization is a promising procedure for the treatment of adolescents' immature permanent teeth with necrotic pulp and/or apical periodontitis. However, the ability to successfully perform pulp revascularization in a middle-aged patient remains unclear. A 39-year-old woman was referred for treatment of teet...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.04.022
更新日期:2015-09-01 00:00:00
abstract::This report describes the case of a patient with mesenchymal chondrosarcoma in the region of tooth #18 that mimicked apical periodontitis. Forty-five days after endodontic treatment, gingival swelling and intense pain were observed. The endodontist suspected a furcation lesion, and the treatment option that the patien...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.08.016
更新日期:2008-11-01 00:00:00
abstract:INTRODUCTION:The objective of this study was to investigate the association between chronic apical periodontitis (CAP) and low-birth-weight preterm births (LBWPB). METHODS:Sixty-three women in postpartum period were included in this case-control study. The case group consisted of mothers of LBWPB infants (n = 33), and...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.11.018
更新日期:2015-03-01 00:00:00
abstract::The purpose of this study was to develop a method for lidocaine detection in dental pulp by high-performance liquid chromatography. The amounts of lidocaine in dog pulps were quantitated after local injection to evaluate lidocaine recovery from pulp tissue with this technique. Comparison was also made between the amou...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200101000-00009
更新日期:2001-01-01 00:00:00
abstract::The authors reviewed the literature evaluating the antibacterial effectiveness of intracanal medicaments used in the management of apical periodontitis. A PICO (problem, intervention, comparison, outcome) strategy was developed to identify studies dealing with calcium hydroxide, phenolic derivatives, iodine-potassium ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000129959.20011.ee
更新日期:2004-10-01 00:00:00
abstract:INTRODUCTION:Clinicians commonly mix mineral trioxide aggregate (MTA) to a desired consistency rather than use the recommended amounts of powder and water. The purpose of this in vitro study was to evaluate how varying the water-to-powder (WP) ratio affects the setting expansion of white MTA (WMTA) and gray MTA (GMTA)....
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2010.03.010
更新日期:2010-08-01 00:00:00
abstract::The difference in resistance to bacterial invasion into the dentinal tubules between vital and nonvital teeth has not been determined. This study was conducted to clarify the effect of vital pulp on bacterial invasion into the dentinal tubules. The specimens were 19 intact pairs of bilateral upper third molars of 19 h...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81098-8
更新日期:1995-02-01 00:00:00
abstract::The purpose of this study was to compare the reinforcement and strengthening ability of Resilon, gutta-percha, and a self-curing composite resin (Bisfil 2B) in endodontically treated roots of immature teeth. Sixty single rooted teeth were divided into five groups of 12 teeth each. Teeth in all groups except the negati...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.08.001
更新日期:2006-04-01 00:00:00
abstract::Vascular casts of cat premolar pulps in various stages of growth were made by injection of low-viscosity resin into the pulpal blood vessels. Examination under a scanning electron microscope revealed that, in the young pulp, the main arterioles ascend in the center of the pulp and venules run along the side of the roo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(89)80028-7
更新日期:1989-10-01 00:00:00
abstract:INTRODUCTION:Apical surgery is frequently indicated in maxillary first molars. Occasionally, a vascular anastomosis in the lateral maxillary sinus wall can be observed during surgery. The aim of this study was to examine the distance between the vascular bone channel (VBC) and the root apices of maxillary first molars ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.09.004
更新日期:2019-12-01 00:00:00
abstract:INTRODUCTION:The objective of this study was to evaluate the pH and calcium ion release of calcium hydroxide pastes associated with different substances. METHODS:Forty acrylic teeth with simulated root canals were divided into 4 groups according to the substance associated to the calcium hydroxide paste: chlorhexidine...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.05.009
更新日期:2009-09-01 00:00:00
abstract::The structure of mechanically instrumented intraradicular dentin after irrigation with NaOCl as the initial rinse and BioPure MTAD as the final rinse were examined from the coronal, middle, and apical parts of root canal walls using transmission electron microscopy. Sterile distilled water and EDTA as final rinses wer...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.10.035
更新日期:2006-03-01 00:00:00
abstract::This study investigated possible collateral C-fiber innervation between the pulps of rat molars by assessing neurogenic inflammation (NI) induced by the C-fiber excitant mustard oil (MO). MO was applied to the pulp of the left mandibular first molar in two groups of rats: group 1, guanethidine sympathectomized rats (t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(96)80242-1
更新日期:1996-08-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to determine the prevalence and characteristics of postoperative pain after endodontic microsurgery and to identify potential predictors for severe pain. METHODS:One hundred seventy-three patients who underwent endodontic microsurgery at a private practice were included in the st...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.12.012
更新日期:2020-12-23 00:00:00
abstract:INTRODUCTION:The purpose of this study was to analyze the features of lesions obtained from biopsies at the periapical area of teeth with a radiographic or clinical initial diagnosis of apical periodontitis. METHODS:A retrospective study was conducted on biopsies obtained from 1953-2018 at 3 Brazilian oral and maxillo...
journal_title:Journal of endodontics
pub_type: 杂志文章,多中心研究
doi:10.1016/j.joen.2020.01.003
更新日期:2020-04-01 00:00:00
abstract::Ultrasonic instruments are a valuable asset for removing intraradicular posts from root canals before nonsurgical endodontic therapy. The purpose of this study was to evaluate the relative efficacy of post removal by ultrasonic and sonic devices. Fifty extracted teeth were instrumented and obturated. The canals were p...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)80044-0
更新日期:1994-10-01 00:00:00
abstract::Periapical lesions usually are caused by root canal infection; nevertheless, other pathologies may eventually involve the tooth apex, making the correct diagnosis more difficult. Glandular odontogenic cysts (GOCs) are uncommon and, despite their cystic nature, may present an aggressive behavior and a high recurrence r...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.06.015
更新日期:2017-11-01 00:00:00
abstract:INTRODUCTION:This study evaluated the success rate of and tooth substance removal required for computer-guided preparation of endodontic access cavities. METHODS:Thirty acrylic typodont teeth with root canals (10 each of tooth numbers 11, 14, and 17) were randomly allocated to a study or control group (15 teeth per gr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.10.025
更新日期:2021-02-01 00:00:00
abstract:INTRODUCTION:The aim of the present study was to assess the bond strength of root fillings in oval-shaped canals prepared with the self-adjusting file (SAF) system. METHODS:A careful specimen selection resulted in 2 equal groups, each consisting of 12 extracted mandibular canines with oval canals that had vital pulps ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.10.026
更新日期:2013-02-01 00:00:00
abstract::Enterococcus faecalis has been suggested to be an important etiological agent in endodontic failures. The purpose of this study was to evaluate the effectiveness of chlorhexidine- or antibiotics-based medications in eliminating E. faecalis biofilms. One-day and three-day biofilms of E. faecalis were induced on cellulo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200110000-00004
更新日期:2001-10-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to assess the ex vivo torsional performance of a novel rotary system in small root canals after 2 different glide path preparations. METHODS:Each independent canal of 8 mesial roots of mandibular molars was randomly assigned to achieve a reproducible glide path with a new set...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.10.034
更新日期:2017-03-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to establish a stable experimental mice pulpal inflammatory model and to evaluate inflammatory reactions of pulpal tissue after pulpal exposure. METHODS:Pulpal inflammation was induced in 80 C57BL/6 mice by occlusal exposure of the pulp of the maxillary first molar. The mice ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.09.003
更新日期:2017-01-01 00:00:00
abstract::The purpose of this study was to evaluate the durability of insulated probes designed to be used with the Endocater Apex Locator when put through multiple cleaning and autoclaving cycles. The manufacturer has reported that the plastic coating on the probes is reliable with normal use and sterilizable up to a maximum o...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81502-5
更新日期:1993-08-01 00:00:00
abstract:INTRODUCTION:Various materials have been advocated for use as root-end filling materials. The purpose of the present in vitro study was to compare the cytotoxicity of 4 root-end filling materials: glass ionomer cement (GIC; Fuji II, GC Corp, Tokyo, Japan), reinforced zinc oxide-eugenol cement (IRM; Dentsply Tulsa Denta...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.09.004
更新日期:2012-12-01 00:00:00