Abstract:
BACKGROUND:Low iron bioavailability is a common feature of ocean surface water and therefore micro-algae developed original strategies to optimize iron uptake and metabolism. The marine picoeukaryotic green alga Ostreococcus tauri is a very good model for studying physiological and genetic aspects of the adaptation of the green algal lineage to the marine environment: it has a very compact genome, is easy to culture in laboratory conditions, and can be genetically manipulated by efficient homologous recombination. In this study, we aimed at characterizing the mechanisms of iron assimilation in O. tauri by combining genetics and physiological tools. Specifically, we wanted to identify and functionally characterize groups of genes displaying tightly orchestrated temporal expression patterns following the exposure of cells to iron deprivation and day/night cycles, and to highlight unique features of iron metabolism in O. tauri, as compared to the freshwater model alga Chalamydomonas reinhardtii. RESULTS:We used RNA sequencing to investigated the transcriptional responses to iron limitation in O. tauri and found that most of the genes involved in iron uptake and metabolism in O. tauri are regulated by day/night cycles, regardless of iron status. O. tauri lacks the classical components of a reductive iron uptake system, and has no obvious iron regulon. Iron uptake appears to be copper-independent, but is regulated by zinc. Conversely, iron deprivation resulted in the transcriptional activation of numerous genes encoding zinc-containing regulation factors. Iron uptake is likely mediated by a ZIP-family protein (Ot-Irt1) and by a new Fea1-related protein (Ot-Fea1) containing duplicated Fea1 domains. The adaptation of cells to iron limitation involved an iron-sparing response tightly coordinated with diurnal cycles to optimize cell functions and synchronize these functions with the day/night redistribution of iron orchestrated by ferritin, and a stress response based on the induction of thioredoxin-like proteins, of peroxiredoxin and of tesmin-like methallothionein rather than ascorbate. We briefly surveyed the metabolic remodeling resulting from iron deprivation. CONCLUSIONS:The mechanisms of iron uptake and utilization by O. tauri differ fundamentally from those described in C. reinhardtii. We propose this species as a new model for investigation of iron metabolism in marine microalgae.
journal_name
BMC Genomicsjournal_title
BMC genomicsauthors
Lelandais G,Scheiber I,Paz-Yepes J,Lozano JC,Botebol H,Pilátová J,Žárský V,Léger T,Blaiseau PL,Bowler C,Bouget FY,Camadro JM,Sutak R,Lesuisse Edoi
10.1186/s12864-016-2666-6subject
Has Abstractpub_date
2016-05-03 00:00:00pages
319issn
1471-2164pii
10.1186/s12864-016-2666-6journal_volume
17pub_type
杂志文章相关文献
BMC GENOMICS文献大全abstract:BACKGROUND:The Iberian lynx (Lynx pardinus) has been acknowledged as the most endangered felid species in the world. An intense contraction and fragmentation during the twentieth century left less than 100 individuals split in two isolated and genetically eroded populations by 2002. Genetic monitoring and management so...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-3946-5
更新日期:2017-07-21 00:00:00
abstract:BACKGROUND:The term pan-genome was proposed to denominate collections of genomic sequences jointly analyzed or used as a reference. The constant growth of genomic data intensifies development of data structures and algorithms to investigate pan-genomes efficiently. RESULTS:This work focuses on providing a tool for dis...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-6610-4
更新日期:2020-04-16 00:00:00
abstract:BACKGROUND:The contribution of genetic factors to the development of breast cancer in the admixed and consanguineous population of the western region of Saudi Arabia is thought to be significant as the disease is early onset. The current protocols of continuous clinical follow-up of relatives of such patients are costl...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-16-S1-S3
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:The hepatitis E virus (HEV) is the causative pathogen of hepatitis E, a global public health concern. HEV comprises 8 genotypes with a wide host range and geographic distribution. This study aims to determine the genetic factors influencing the molecular adaptive changes of HEV open reading frames (ORFs) and...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-6100-8
更新日期:2019-10-29 00:00:00
abstract:BACKGROUND:It is believed that the main factors of low prenatal growth in mammals are genetic and environmental. We used isogenic mice maintained in standard conditions to analyze how natural non-genetic microsomia (low birth weight) is produced in inbred mice and its long term effect on health. To better understand th...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-327
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND:The principal toxicity of acute organophosphate (OP) pesticide poisoning is the disruption of neurotransmission through inhibition of acetylcholinesterase (AChE). However, other mechanisms leading to persistent effects and neurodegeneration remain controversial and difficult to detect. Because Caenorhabditis...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-291
更新日期:2013-04-30 00:00:00
abstract:BACKGROUND:Modern broiler chickens exhibit very rapid growth and high feed efficiency compared to unselected chicken breeds. The improved production efficiency in modern broiler chickens was achieved by the intensive genetic selection for meat production. This study was designed to investigate the genetic alterations a...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-3471-y
更新日期:2017-01-13 00:00:00
abstract:BACKGROUND:RNA amplification is necessary for profiling gene expression from small tissue samples. Previous studies have shown that the T7 based amplification techniques are reproducible but may distort the true abundance of targets. However, the consequences of such distortions on the ability to detect biological vari...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-7-252
更新日期:2006-10-09 00:00:00
abstract:BACKGROUND:Quercetin is a polyphenol of great interest given its antioxidant activity and involvement in the immune response. Although quercetin has been well studied at the molecular level as a gene regulator and an activator of specific cellular pathways, not much attention has been given to its mechanism of action a...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-5966-9
更新日期:2019-07-23 00:00:00
abstract:BACKGROUND:Infectious laryngotracheitis virus (ILTV; gallid herpesvirus 1) infection causes high mortality and huge economic losses in the poultry industry. To protect chickens against ILTV infection, chicken-embryo origin (CEO) and tissue-culture origin (TCO) vaccines have been used. However, the transmission of vacci...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-143
更新日期:2012-04-24 00:00:00
abstract:BACKGROUND:Staphylococcus aureus or MRSA (Methicillin Resistant S. aureus), is an acquired pathogen and the primary cause of nosocomial infections worldwide. In S. aureus, teichoic acid is an essential component of the cell wall, and its biosynthesis is not yet well characterized. Studies in Bacillus subtilis have disc...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-7-74
更新日期:2006-04-05 00:00:00
abstract:BACKGROUND:Polypyrimidine-tract binding proteins (PTBs) are ubiquitous RNA-binding proteins in plants and animals that play diverse role in RNA metabolic processes. PTB proteins bind to target RNAs through motifs rich in cytosine/uracil residues to fine-tune transcript metabolism. Among tuber and root crops, potato has...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-4502-7
更新日期:2018-02-07 00:00:00
abstract:BACKGROUND:Pantoea spp. are frequently isolated from a wide range of ecological niches and have various biological roles, as plant epi- or endophytes, biocontrol agents, plant-growth promoters or as pathogens of both plant and animal hosts. This suggests that members of this genus have undergone extensive genotypic div...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-625
更新日期:2012-11-15 00:00:00
abstract:BACKGROUND:S. aureus is one of the main pathogens responsible for the intra-mammary infection in dairy ruminants. Although much work has been carried out to understand the complex physiological and cellular events that occur in the mammary gland in response to S. aureus, the protective mechanisms are still poorly under...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-540
更新日期:2012-10-09 00:00:00
abstract:BACKGROUND:Drought is a lifestyle disease. Plant metabolomics has been exercised for understanding the fine-tuning of the potential pathways to surmount the adverse effects of drought stress. A broad spectrum of morphological and metabolic responses from seven Triticeae species including wild types with different droug...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-4321-2
更新日期:2017-12-15 00:00:00
abstract:BACKGROUND:Despite the recent identification of several prognostic gene signatures, the lack of common genes among experimental cohorts has posed a considerable challenge in uncovering the molecular basis underlying hepatocellular carcinoma (HCC) recurrence for application in clinical purposes. To overcome the limitati...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1472-x
更新日期:2015-04-10 00:00:00
abstract:BACKGROUND:The mitochondrial genomes of higher plants vary remarkably in size, structure and sequence content, as demonstrated by the accumulation and activity of repetitive DNA sequences. Incompatibility between mitochondrial genome and nuclear genome leads to non-functional male reproductive organs and results in cyt...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-5122-y
更新日期:2018-10-26 00:00:00
abstract:BACKGROUND:Alternative σ factors are important transcriptional regulators in bacteria. While σ(B) has been shown to control a large regulon and play important roles in stress response and virulence in the pathogen Listeria monocytogenes, the function of σ(H) has not yet been well defined in Listeria, even though σ(H) c...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-2432-9
更新日期:2016-02-16 00:00:00
abstract:BACKGROUND:The ascomycete fungus Ceratocystis cacaofunesta is the causal agent of wilt disease in cacao, which results in significant economic losses in the affected producing areas. Despite the economic importance of the Ceratocystis complex of species, no genomic data are available for any of its members. Given that ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-91
更新日期:2013-02-11 00:00:00
abstract:BACKGROUND:Cowpea, Vigna unguiculata (L.) Walp., is one of the most important food and forage legumes in the semi-arid tropics because of its drought tolerance and ability to grow on poor quality soils. Approximately 80% of cowpea production takes place in the dry savannahs of tropical West and Central Africa, mostly b...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-9-103
更新日期:2008-02-27 00:00:00
abstract:BACKGROUND:One of the key characters of social insects is the division of labor, in which different tasks are allocated to various castes. In termites, one of the representative groups of social insects, morphological differences as well as behavioral differences can be recognized among castes. However, very little is ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-314
更新日期:2010-05-20 00:00:00
abstract:BACKGROUND:Cottonseed is one of the most important raw materials for plant protein, oil and alternative biofuel for diesel engines. Understanding the complex genetic basis of cottonseed traits is requisite for achieving efficient genetic improvement of the traits. However, it is not yet clear about their genetic archit...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-4837-0
更新日期:2018-06-13 00:00:00
abstract:BACKGROUND:Magnesium (Mg)-deficiency is frequently observed in Citrus plantations and is responsible for the loss of productivity and poor fruit quality. Knowledge on the effects of Mg-deficiency on upstream targets is scarce. Seedlings of 'Xuegan' [Citrus sinensis (L.) Osbeck] were irrigated with Mg-deficient (0 mM Mg...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1462-z
更新日期:2015-03-31 00:00:00
abstract:BACKGROUND:Biocontrol of bacterial pathogens by bacteriophages (phages) represents a promising strategy. Vibrio alginolyticus, a gram-negative bacterium, is a notorious pathogen responsible for the loss of economically important farmed marine animals. To date, few V. alginolyticus phages have been successfully isolated...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-5131-x
更新日期:2018-10-11 00:00:00
abstract:BACKGROUND:The giant panda, one of the most primitive carnivores, is an endangered animal. Although it has been the subject of many interesting studies during recent years, little is known about its genome. In order to promote research on this genome, a bacterial artificial chromosome (BAC) library of the giant panda w...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-7-294
更新日期:2006-11-17 00:00:00
abstract:BACKGROUND:The ascomycete fungus Zymoseptoria tritici (synonyms: Mycosphaerella graminicola, Septoria tritici) is a major pathogen of wheat that causes the economically important foliar disease Septoria tritici blotch. Despite its importance as a pathogen, little is known about the reaction of this fungus to light. To ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-06899-y
更新日期:2020-07-25 00:00:00
abstract:BACKGROUND:Many bacteria can deliver pathogenic proteins (effectors) through type IV secretion systems (T4SSs) to eukaryotic cytoplasm, causing host diseases. The inherent property, such as sequence diversity and global scattering throughout the whole genome, makes it a big challenge to effectively identify the full se...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-50
更新日期:2014-01-21 00:00:00
abstract:BACKGROUND:The process of alternative splicing provides a unique mechanism by which eukaryotes are able to produce numerous protein products from the same gene. Heightened variability in the proteome has been thought to potentiate increased behavioral complexity and response flexibility to environmental stimuli, thus c...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-6600-6
更新日期:2020-03-23 00:00:00
abstract:BACKGROUND:Genetic association studies that seek to explain the inheritance of complex traits typically fail to explain a majority of the heritability of the trait under study. Thus, we are left with a gap in the map from genotype to phenotype. Several approaches have been used to fill this gap, including those that at...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-6739-1
更新日期:2020-05-04 00:00:00
abstract:BACKGROUND:Foraminiferan protists, which are significant players in most marine ecosystems, are also genetic innovators, harboring unique modifications to proteins that make up the basic eukaryotic cell machinery. Despite their ecological and evolutionary importance, foraminiferan genomes are poorly understood due to t...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-12-169
更新日期:2011-03-31 00:00:00