Complete genomic sequence of the Vibrio alginolyticus bacteriophage Vp670 and characterization of the lysis-related genes, cwlQ and holA.

Abstract:

BACKGROUND:Biocontrol of bacterial pathogens by bacteriophages (phages) represents a promising strategy. Vibrio alginolyticus, a gram-negative bacterium, is a notorious pathogen responsible for the loss of economically important farmed marine animals. To date, few V. alginolyticus phages have been successfully isolated, and only three complete genome sequences of them have been released. The limited available phage resources and poor genomic data hamper research on V. alginolyticus phages and their applications for the biocontrol of V. alginolyticus. RESULTS:We isolated a phage, Vp670, against the V. alginolyticus strain E06333 and obtained its full genomic sequence. It contains 43,121 nucleotides with a GC content of 43.4%, and codes for 49 predicted open reading frames. Observation by electron microscope combined with phylogenetic analysis of DNA polymerase indicates that Vp670 belongs to the subfamily Autographivirinae in the family Podoviridae. orf3 (designated holA) and orf8 (designated cwlQ) are predicted to encode a holin (HolA) and an endolysin (CwlQ), respectively. Expression of holA alone or coexpression of holA and cwlQ from within arrested the growth of Escherichia coli and V. alginolyticus while the expression of cwlQ alone had no effect on the growth of them. Further observation by transmission electron microscopy revealed that the expression of holA vanished the outer membrane and caused the release of cellular contents of V. alginolyticus and the coexpression of holA and cwlQ directly burst the cells and caused a more drastic release of cellular contents. Expression of cwlQ alone in V. alginolyticus did not cause cytomorphological changes. CONCLUSIONS:Phage Vp670 is a V. alginolyticus phage belonging to the family of Podoviridae. The genome of Vp670 contains a two-component lysis module, which is comprised of holA and cwlQ. holA is predicted to encode for the holin protein, HolA, and cwlQ is predicted to encode for the endolysin protein, CwlQ. Both holA and cwlQ likely play important roles during the release of phage progeny.

journal_name

BMC Genomics

journal_title

BMC genomics

authors

Luo P,Yun L,Li Y,Tian Y,Liu Q,Huang W,Hu C

doi

10.1186/s12864-018-5131-x

subject

Has Abstract

pub_date

2018-10-11 00:00:00

pages

741

issue

1

issn

1471-2164

pii

10.1186/s12864-018-5131-x

journal_volume

19

pub_type

杂志文章
  • Genome and Transcriptome sequence of Finger millet (Eleusine coracana (L.) Gaertn.) provides insights into drought tolerance and nutraceutical properties.

    abstract:BACKGROUND:Finger millet (Eleusine coracana (L.) Gaertn.) is an important staple food crop widely grown in Africa and South Asia. Among the millets, finger millet has high amount of calcium, methionine, tryptophan, fiber, and sulphur containing amino acids. In addition, it has C4 photosynthetic carbon assimilation mech...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-017-3850-z

    authors: Hittalmani S,Mahesh HB,Shirke MD,Biradar H,Uday G,Aruna YR,Lohithaswa HC,Mohanrao A

    更新日期:2017-06-15 00:00:00

  • Unravelling the complex trait of harvest index in rapeseed (Brassica napus L.) with association mapping.

    abstract:BACKGROUND:Harvest index (HI), the ratio of grain yield to total biomass, is considered as a measure of biological success in partitioning assimilated photosynthate to the harvestable product. While crop production can be dramatically improved by increasing HI, the underlying molecular genetic mechanism of HI in rapese...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-015-1607-0

    authors: Luo X,Ma C,Yue Y,Hu K,Li Y,Duan Z,Wu M,Tu J,Shen J,Yi B,Fu T

    更新日期:2015-05-12 00:00:00

  • Integrated proteomic and metabolomic analysis to study the effects of spaceflight on Candida albicans.

    abstract:BACKGROUND:Candida albicans is an opportunistic pathogenic yeast, which could become pathogenic in various stressful environmental factors including the spaceflight environment. In this study, we aim to explore the phenotypic changes and possible mechanisms of C. albicans after exposure to spaceflight conditions. RESU...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-6476-5

    authors: Wang J,Liu Y,Zhao G,Gao J,Liu J,Wu X,Xu C,Li Y

    更新日期:2020-01-17 00:00:00

  • Development of EST-based SNP and InDel markers and their utilization in tetraploid cotton genetic mapping.

    abstract:BACKGROUND:Availability of molecular markers has proven to be an efficient tool in facilitating progress in plant breeding, which is particularly important in the case of less researched crops such as cotton. Considering the obvious advantages of single nucleotide polymorphisms (SNPs) and insertion-deletion polymorphis...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-1046

    authors: Li X,Gao W,Guo H,Zhang X,Fang DD,Lin Z

    更新日期:2014-12-01 00:00:00

  • A generally applicable validation scheme for the assessment of factors involved in reproducibility and quality of DNA-microarray data.

    abstract:BACKGROUND:In research laboratories using DNA-microarrays, usually a number of researchers perform experiments, each generating possible sources of error. There is a need for a quick and robust method to assess data quality and sources of errors in DNA-microarray experiments. To this end, a novel and cost-effective val...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-6-77

    authors: van Hijum SA,de Jong A,Baerends RJ,Karsens HA,Kramer NE,Larsen R,den Hengst CD,Albers CJ,Kok J,Kuipers OP

    更新日期:2005-05-20 00:00:00

  • SeAMotE: a method for high-throughput motif discovery in nucleic acid sequences.

    abstract:BACKGROUND:The large amount of data produced by high-throughput sequencing poses new computational challenges. In the last decade, several tools have been developed for the identification of transcription and splicing factor binding sites. RESULTS:Here, we introduce the SeAMotE (Sequence Analysis of Motifs Enrichment)...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-925

    authors: Agostini F,Cirillo D,Ponti RD,Tartaglia GG

    更新日期:2014-10-23 00:00:00

  • Alternative splicing enriched cDNA libraries identify breast cancer-associated transcripts.

    abstract:BACKGROUND:Alternative splicing (AS) is a central mechanism in the generation of genomic complexity and is a major contributor to transcriptome and proteome diversity. Alterations of the splicing process can lead to deregulation of crucial cellular processes and have been associated with a large spectrum of human disea...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-S5-S4

    authors: Ferreira EN,Rangel MC,Galante PF,de Souza JE,Molina GC,de Souza SJ,Carraro DM

    更新日期:2010-12-22 00:00:00

  • H2A.Z marks antisense promoters and has positive effects on antisense transcript levels in budding yeast.

    abstract:BACKGROUND:The histone variant H2A.Z, which has been reported to have both activating and repressive effects on gene expression, is known to occupy nucleosomes at the 5' ends of protein-coding genes. RESULTS:We now find that H2A.Z is also significantly enriched in gene coding regions and at the 3' ends of genes in bud...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-015-1247-4

    authors: Gu M,Naiyachit Y,Wood TJ,Millar CB

    更新日期:2015-02-19 00:00:00

  • Effort required to finish shotgun-generated genome sequences differs significantly among vertebrates.

    abstract:BACKGROUND:The approaches for shotgun-based sequencing of vertebrate genomes are now well-established, and have resulted in the generation of numerous draft whole-genome sequence assemblies. In contrast, the process of refining those assemblies to improve contiguity and increase accuracy (known as 'sequence finishing')...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-21

    authors: Blakesley RW,Hansen NF,Gupta J,McDowell JC,Maskeri B,Barnabas BB,Brooks SY,Coleman H,Haghighi P,Ho SL,Schandler K,Stantripop S,Vogt JL,Thomas PJ,NISC Comparative Sequencing Program.,Bouffard GG,Green ED

    更新日期:2010-01-11 00:00:00

  • Histone H3 lysine 4 methyltransferase is required for facultative heterochromatin at specific loci.

    abstract:BACKGROUND:Histone H3 lysine 4 tri-methylation (H3K4me3) and histone H3 lysine 9 tri-methylation (H3K9me3) are widely perceived to be opposing and often mutually exclusive chromatin modifications. However, both are needed for certain light-activated genes in Neurospora crassa (Neurospora), including frequency (frq) and...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-019-5729-7

    authors: Zhu Q,Ramakrishnan M,Park J,Belden WJ

    更新日期:2019-05-08 00:00:00

  • A comparison of Illumina and Ion Torrent sequencing platforms in the context of differential gene expression.

    abstract:BACKGROUND:Though Illumina has largely dominated the RNA-Seq field, the simultaneous availability of Ion Torrent has left scientists wondering which platform is most effective for differential gene expression (DGE) analysis. Previous investigations of this question have typically used reference samples derived from cel...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-017-4011-0

    authors: Lahens NF,Ricciotti E,Smirnova O,Toorens E,Kim EJ,Baruzzo G,Hayer KE,Ganguly T,Schug J,Grant GR

    更新日期:2017-08-10 00:00:00

  • Screening populations for copy number variation using genotyping-by-sequencing: a proof of concept using soybean fast neutron mutants.

    abstract:BACKGROUND:The effective use of mutant populations for reverse genetic screens relies on the population-wide characterization of the induced mutations. Genome- and population-wide characterization of the mutations found in fast neutron populations has been hindered, however, by the wide range of mutations generated and...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-019-5998-1

    authors: Lemay MA,Torkamaneh D,Rigaill G,Boyle B,Stec AO,Stupar RM,Belzile F

    更新日期:2019-08-06 00:00:00

  • Transcriptome profiles of Quercus rubra responding to increased O3 stress.

    abstract:BACKGROUND:Climate plays an essential role in forest health, and climate change may increase forest productivity losses due to abiotic and biotic stress. Increased temperature leads to the increased formation of ozone (O3). Ozone is formed by the interaction of sunlight, molecular oxygen and by the reactions of chemica...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-6549-5

    authors: Soltani N,Best T,Grace D,Nelms C,Shumaker K,Romero-Severson J,Moses D,Schuster S,Staton M,Carlson J,Gwinn K

    更新日期:2020-02-14 00:00:00

  • Transcriptomic and metabolomic profiling of chicken adipose tissue in response to insulin neutralization and fasting.

    abstract:BACKGROUND:Domestic broiler chickens rapidly accumulate adipose tissue due to intensive genetic selection for rapid growth and are naturally hyperglycemic and insulin resistant, making them an attractive addition to the suite of rodent models used for studies of obesity and type 2 diabetes in humans. Furthermore, chick...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-13-441

    authors: Ji B,Ernest B,Gooding JR,Das S,Saxton AM,Simon J,Dupont J,Métayer-Coustard S,Campagna SR,Voy BH

    更新日期:2012-08-31 00:00:00

  • Identification of chilling stress-responsive tomato microRNAs and their target genes by high-throughput sequencing and degradome analysis.

    abstract:BACKGROUND:MicroRNAs (miRNAs) are a class of noncoding small RNAs (sRNAs) that are 20-24 nucleotides (nt) in length. Extensive studies have indicated that miRNAs play versatile roles in plants, functioning in processes such as growth, development and stress responses. Chilling is a common abiotic stress that seriously ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-1130

    authors: Cao X,Wu Z,Jiang F,Zhou R,Yang Z

    更新日期:2014-12-17 00:00:00

  • A comparative genomic analysis of putative pathogenicity genes in the host-specific sibling species Colletotrichum graminicola and Colletotrichum sublineola.

    abstract:BACKGROUND:Colletotrichum graminicola and C. sublineola cause anthracnose leaf and stalk diseases of maize and sorghum, respectively. In spite of their close evolutionary relationship, the two species are completely host-specific. Host specificity is often attributed to pathogen virulence factors, including specialized...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-3457-9

    authors: Buiate EAS,Xavier KV,Moore N,Torres MF,Farman ML,Schardl CL,Vaillancourt LJ

    更新日期:2017-01-10 00:00:00

  • Highly sensitive amplicon-based transcript quantification by semiconductor sequencing.

    abstract:BACKGROUND:In clinical and basic research custom panels for transcript profiling are gaining importance because only project specific informative genes are interrogated. This approach reduces costs and complexity of data analysis and allows multiplexing of samples. Polymerase-chain-reaction (PCR) based TaqMan assays ha...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-565

    authors: Zhang JD,Schindler T,Küng E,Ebeling M,Certa U

    更新日期:2014-07-05 00:00:00

  • Polymorphism discovery and allele frequency estimation using high-throughput DNA sequencing of target-enriched pooled DNA samples.

    abstract:BACKGROUND:The central role of the somatotrophic axis in animal post-natal growth, development and fertility is well established. Therefore, the identification of genetic variants affecting quantitative traits within this axis is an attractive goal. However, large sample numbers are a pre-requisite for the identificati...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-13-16

    authors: Mullen MP,Creevey CJ,Berry DP,McCabe MS,Magee DA,Howard DJ,Killeen AP,Park SD,McGettigan PA,Lucy MC,Machugh DE,Waters SM

    更新日期:2012-01-11 00:00:00

  • Haplotyping and copy number estimation of the highly polymorphic human beta-defensin locus on 8p23 by 454 amplicon sequencing.

    abstract:BACKGROUND:The beta-defensin gene cluster (DEFB) at chromosome 8p23.1 is one of the most copy number (CN) variable regions of the human genome. Whereas individual DEFB CNs have been suggested as independent genetic risk factors for several diseases (e.g. psoriasis and Crohn's disease), the role of multisite sequence va...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-252

    authors: Taudien S,Groth M,Huse K,Petzold A,Szafranski K,Hampe J,Rosenstiel P,Schreiber S,Platzer M

    更新日期:2010-04-19 00:00:00

  • Genome sequence of an aflatoxigenic pathogen of Argentinian peanut, Aspergillus arachidicola.

    abstract:BACKGROUND:Aspergillus arachidicola is an aflatoxigenic fungal species, first isolated from the leaves of a wild peanut species native to Argentina. It has since been reported in maize, Brazil nut and human sputum samples. This aflatoxigenic species is capable of secreting both B and G aflatoxins, similar to A. parasit...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-4576-2

    authors: Moore GG,Mack BM,Beltz SB,Puel O

    更新日期:2018-03-09 00:00:00

  • Host specialization of the blast fungus Magnaporthe oryzae is associated with dynamic gain and loss of genes linked to transposable elements.

    abstract:BACKGROUND:Magnaporthe oryzae (anamorph Pyricularia oryzae) is the causal agent of blast disease of Poaceae crops and their wild relatives. To understand the genetic mechanisms that drive host specialization of M. oryzae, we carried out whole genome resequencing of four M. oryzae isolates from rice (Oryza sativa), one ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2690-6

    authors: Yoshida K,Saunders DG,Mitsuoka C,Natsume S,Kosugi S,Saitoh H,Inoue Y,Chuma I,Tosa Y,Cano LM,Kamoun S,Terauchi R

    更新日期:2016-05-18 00:00:00

  • Xylem transcription profiles indicate potential metabolic responses for economically relevant characteristics of Eucalyptus species.

    abstract:BACKGROUND:Eucalyptus is one of the most important sources of industrial cellulose. Three species of this botanical group are intensively used in breeding programs: E. globulus, E. grandis and E. urophylla. E. globulus is adapted to subtropical/temperate areas and is considered a source of high-quality cellulose; E. gr...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-14-201

    authors: Salazar MM,Nascimento LC,Camargo EL,Gonçalves DC,Lepikson Neto J,Marques WL,Teixeira PJ,Mieczkowski P,Mondego JM,Carazzolle MF,Deckmann AC,Pereira GA

    更新日期:2013-03-22 00:00:00

  • Comparative venom gland transcriptome analysis of the scorpion Lychas mucronatus reveals intraspecific toxic gene diversity and new venomous components.

    abstract:BACKGROUND:Lychas mucronatus is one scorpion species widely distributed in Southeast Asia and southern China. Anything is hardly known about its venom components, despite the fact that it can often cause human accidents. In this work, we performed a venomous gland transcriptome analysis by constructing and screening th...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-452

    authors: Ruiming Z,Yibao M,Yawen H,Zhiyong D,Yingliang W,Zhijian C,Wenxin L

    更新日期:2010-07-28 00:00:00

  • Glycogenome expression dynamics during mouse C2C12 myoblast differentiation suggests a sequential reorganization of membrane glycoconjugates.

    abstract:BACKGROUND:Several global transcriptomic and proteomic approaches have been applied in order to obtain new molecular insights on skeletal myogenesis, but none has generated any specific data on glycogenome expression, and thus on the role of glycan structures in this process, despite the involvement of glycoconjugates ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-10-483

    authors: Janot M,Audfray A,Loriol C,Germot A,Maftah A,Dupuy F

    更新日期:2009-10-20 00:00:00

  • cnvScan: a CNV screening and annotation tool to improve the clinical utility of computational CNV prediction from exome sequencing data.

    abstract:BACKGROUND:With advances in next generation sequencing technology and analysis methods, single nucleotide variants (SNVs) and indels can be detected with high sensitivity and specificity in exome sequencing data. Recent studies have demonstrated the ability to detect disease-causing copy number variants (CNVs) in exome...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2374-2

    authors: Samarakoon PS,Sorte HS,Stray-Pedersen A,Rødningen OK,Rognes T,Lyle R

    更新日期:2016-01-14 00:00:00

  • Chips and tags suggest plant-environment interactions differ for two alpine Pachycladon species.

    abstract:BACKGROUND:Expression profiling has been proposed as a means for screening non-model organisms in their natural environments to identify genes potentially important in adaptive diversification. Tag profiling using high throughput sequencing is a relatively low cost means of expression profiling with deep coverage. Howe...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-13-322

    authors: Voelckel C,Gruenheit N,Biggs P,Deusch O,Lockhart P

    更新日期:2012-07-19 00:00:00

  • Genomic arrangement of salinity tolerance QTLs in salmonids: a comparative analysis of Atlantic salmon (Salmo salar) with Arctic charr (Salvelinus alpinus) and rainbow trout (Oncorhynchus mykiss).

    abstract:BACKGROUND:Quantitative trait locus (QTL) studies show that variation in salinity tolerance in Arctic charr and rainbow trout has a genetic basis, even though both these species have low to moderate salinity tolerance capacities. QTL were observed to localize to homologous linkage group segments within putative chromos...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-13-420

    authors: Norman JD,Robinson M,Glebe B,Ferguson MM,Danzmann RG

    更新日期:2012-08-24 00:00:00

  • Metabolic modeling and analysis of the metabolic switch in Streptomyces coelicolor.

    abstract:BACKGROUND:The transition from exponential to stationary phase in Streptomyces coelicolor is accompanied by a major metabolic switch and results in a strong activation of secondary metabolism. Here we have explored the underlying reorganization of the metabolome by combining computational predictions based on constrain...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-202

    authors: Alam MT,Merlo ME,STREAM Consortium.,Hodgson DA,Wellington EM,Takano E,Breitling R

    更新日期:2010-03-26 00:00:00

  • "Integrative genomic analysis of the bioprospection of regulators and accessory enzymes associated with cellulose degradation in a filamentous fungus (Trichoderma harzianum)".

    abstract:BACKGROUND:Unveiling fungal genome structure and function reveals the potential biotechnological use of fungi. Trichoderma harzianum is a powerful CAZyme-producing fungus. We studied the genomic regions in T. harzianum IOC3844 containing CAZyme genes, transcription factors and transporters. RESULTS:We used bioinformat...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-07158-w

    authors: Ferreira Filho JA,Horta MAC,Dos Santos CA,Almeida DA,Murad NF,Mendes JS,Sforça DA,Silva CBC,Crucello A,de Souza AP

    更新日期:2020-11-02 00:00:00

  • Variant detection and runs of homozygosity in next generation sequencing data elucidate the genetic background of Lundehund syndrome.

    abstract:BACKGROUND:The Lundehund is a highly specialized breed characterized by a unique flexibility of the joints and polydactyly in all four limbs. The extremely small population size and high inbreeding has promoted a high frequency of diseased dogs affected by the Lundehund syndrome (LS), a severe gastro-enteropathic disea...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2844-6

    authors: Metzger J,Pfahler S,Distl O

    更新日期:2016-08-02 00:00:00