Abstract:
:Nanoparticles (NP) have a tendency to agglomerate after dispersion in physiological media, which can be prevented by the addition of serum. This may however result in modification of the toxic potential of particles due to the formation of protein corona. Our study aimed to analyze the role of serum that is added to improve the dispersion of 10 nm TiO2 NPs on in vitro and in vivo effects following the exposure via the respiratory route. We characterized NP size, surface charge, sedimentation rate, the presence of protein corona and the oxidant-generating capacity after NP dispersion in the presence/absence of serum. The effect of serum on NP internalization, cytotoxicity and pro-inflammatory responses was assessed in a human pulmonary cell line, NCI-H292. Serum in the dispersion medium led to a slower sedimentation, but an enhanced cellular uptake of TiO2 NPs. Despite this greater uptake, the pro-inflammatory response in NCI-H292 cells was lower after serum supplementation (used either as a dispersant or as a cell culture additive), which may be due to a reduced intrinsic oxidative potential of TiO2 NPs. Interestingly, serum could be added 2 h after the NP treatment without affecting the pro-inflammatory response. We also determined the acute pulmonary and hepatic toxicity in vivo 24 h after intratracheal instillation of TiO2 NPs in C57BL/6N mice. The use of serum resulted in an underestimation of the local acute inflammatory response in the lung, while a systemic response on glutathione reduction remained unaffected. In conclusion, serum as a dispersion agent for TiO2 NPs can lead to an underestimation of the acute pro-inflammatory response in vitro and in vivo. To avoid potential unwanted effects of dispersants and medium components, we recommend that the protocol of NM preparation should be thoroughly tested, and reflect as close as possible realistic exposure conditions.
journal_name
Arch Toxicoljournal_title
Archives of toxicologyauthors
Vranic S,Gosens I,Jacobsen NR,Jensen KA,Bokkers B,Kermanizadeh A,Stone V,Baeza-Squiban A,Cassee FR,Tran L,Boland Sdoi
10.1007/s00204-016-1673-3subject
Has Abstractpub_date
2017-01-01 00:00:00pages
353-363issue
1eissn
0340-5761issn
1432-0738pii
10.1007/s00204-016-1673-3journal_volume
91pub_type
杂志文章abstract::Cyclin-dependent kinases (CDKs) are a family of kinases associated predominantly with cell cycle control, making CDK inhibitors interesting candidates for anti-cancer therapeutics. However, retinal toxicity (loss of photoreceptors) has been associated with CDK inhibitors, including the pan-CDK inhibitor AG-012896. The...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-018-2376-8
更新日期:2019-03-01 00:00:00
abstract::The role of the functional substituents on the pyridinium ring of bisquaternary pyridinium compounds, mostly oximes, in exerting reversible and irreversible inhibition of binding of [3H]-N-methyl-4-piperidyl benzilate [( 3H]-4NMPB) to rat brain stem muscarinic receptors was studied. The drugs tested, i.e. HGG-42, HGG-...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00292614
更新日期:1985-10-01 00:00:00
abstract::Cadmium-induced metallothionein (MT) synthesis was investigated in male rats of three ages, 3, 12 and 24 months. Physiological levels of MT in kidney, liver and lung measured simultaneously in untreated animals were found to be within the same range in all three age groups. Following 3-day treatment with CdCl2 equival...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00293629
更新日期:1988-01-01 00:00:00
abstract::Second order rate constants for the initial reaction of 12 mammalian oxyhemoglobins (Hb) with equimolar phenylhydrazine (PHZ), a compound inducing Heinz body hemolytic anemia, were determined by recording continuous changes in absorbance with time at 577 nm. The rate constants were varied in a range from 43 m-1.s-1 wi...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050161
更新日期:1995-01-01 00:00:00
abstract::Teratogenic effects caused by a new nitroimidazopyridazine were examined in Wistar (WU) rats after repeated oral administration of 0, 2.5, 10, and 40 mg/kg, given on days 6-17 post coitum (p.c.) (Day of mating = Day 0) in a regular study on embryo-fetal development according to ICH S5A. At day 20 p.c., fetuses were re...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-006-0057-5
更新日期:2006-07-01 00:00:00
abstract::Changes in the electrophysiologic activity of the rat sciatic nerve were examined after repeated dosing with either of two organophosphate insecticides, parathion, and trichlorfon. Although the parathion treated animals showed overt signs of systemic toxicity, there were no significant changes in any of the measured p...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00333896
更新日期:1983-03-01 00:00:00
abstract::Occupational exposure to benzene has been associated with leukemia, anemia, leukopenia, and thrombocytopenia. Genetic susceptibility to benzene toxicity in humans may be related to variations in benzene metabolizing genes. The main objective of this study was to ascertain whether polymorphism of GSTP1, GSTM1, GSTT1 an...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-017-2104-9
更新日期:2018-06-01 00:00:00
abstract::Age-related macular degeneration (AMD) is a late-onset retinal disease and the leading cause of central vision loss in the elderly. Degeneration of retinal pigment epithelial cells (RPE) is a crucial contributing factor responsible for the onset and progression of AMD. The toxic fluorophore N-retinyl-N-retinylidene et...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-019-02637-w
更新日期:2020-02-01 00:00:00
abstract::The activity of 18 vitamin D analogs on soft tissue calcification and growth impairment in neonatal rats and their effect on bone calcium mobilization, intestinal calcium absorption and binding to intestinal 1,25-dihydroxyvitamin D3 receptors in adult rats were compared. Depending on the chemical modification of the v...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00303129
更新日期:1989-01-01 00:00:00
abstract::Renal papillary necrosis (RPN) was induced in Fischer 344 (F344) rats (n = 4) using 2-bromoethanamine hydrobromide (BEA) dosed at 150 mg/kg, and in multimammate desert mice (Mastomys natalensis) at 150 and 250 mg/kg (n = 4 per group). Control rats and Mastomys were dosed with 0.9% saline (n = 4 per group). Urine was c...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF02733668
更新日期:1995-01-01 00:00:00
abstract::In order to clarify the possible mechanism of hepatocarcinogenesis induced by piperonyl butoxide, we attempted to identify the transcription factor activated by piperonyl butoxide in the male ICR mouse liver. Administration of 0.6% piperonyl butoxide for 24 h elevated the level of liver nuclear proteins that bind to a...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-008-0283-0
更新日期:2008-10-01 00:00:00
abstract::Sarin, a potent organophosphate cholinesterase inhibitor, induces an array of toxic effects including convulsions. Many antidotal treatments contain anticonvulsants to block seizure activity and the ensuing brain damage. Magnesium sulfate (MGS) is used to suppress eclamptic seizures in pregnant women with hypertension...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-012-0916-1
更新日期:2013-02-01 00:00:00
abstract::Current developmental neurotoxicity (DNT) testing in animals faces major limitations, such as high cost and time demands as well as uncertainties in their methodology, evaluation and regulation. Therefore, the use of human-based 3D in vitro systems in combination with high-content image analysis (HCA) might contribute...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-016-1852-2
更新日期:2017-04-01 00:00:00
abstract::Fulminant hepatitis is a serious inflammatory condition of the liver characterized by massive necrosis of liver parenchyma following excessive immune cell infiltration into the liver, and possibly causing sudden hepatic failure and medical emergency. However, the underlying mechanisms are not fully understood. Here, w...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-019-02633-0
更新日期:2020-02-01 00:00:00
abstract::The immunotoxic potential of many classes of environmental contaminants has been well established in laboratory studies, with much attention being focussed on aryl hydrocarbon (Ah)-receptor binding polychlorinated biphenyl (PCB), polychlorinated dibenzo-p-dioxin (PCDD), and polychlorinated dibenzofuran (PCDF) congener...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050326
更新日期:1996-01-01 00:00:00
abstract::A recent intervention study demonstrated the occurrence of irilone as second most abundant isoflavone next to daidzein in human plasma after consumption of a red clover-based dietary supplement (RCDS) containing predominately formononetin ≫ biochanin A > irilone (12 % of these isoflavones). To elucidate the relevance ...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-013-1114-5
更新日期:2014-02-01 00:00:00
abstract::The studies presented were designed to investigate the effects of cytochrome P450 inhibition on tetrahydrofuran-induced hepatocellular proliferation in female B6C3F(1) mice. Groups of female B6C3F(1) mice were exposed to dynamic atmospheres containing tetrahydrofuran (THF) concentrations of 0, 5,400 or 15,000 mg/m(3) ...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-003-0474-7
更新日期:2003-08-01 00:00:00
abstract::The influence of haemoperfusion on some immunological parameters was investigated in a pilot study involving two groups of three patients each. In columns containing cellulose-coated activated charcoal or polystyrene resin a temporary reduction of the total white cell number, particularly involving the segmented and b...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00354774
更新日期:1983-02-01 00:00:00
abstract::Metabolism of carbaryl by rat liver and skin post-mitochondrial fraction has been measured in the presence and absence of cofactors to promote different metabolic pathways. The metabolic capacity was compared with the metabolism of carbaryl during percutaneous absorption in a static skin diffusion system using a varie...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF01973722
更新日期:1991-01-01 00:00:00
abstract::Chlorpyrifos (CPF) is a widely used organophosphorus pesticide. Earlier work from our laboratory and others has demonstrated that the sensitivity to CPF exposure changes markedly during maturation. A number of studies suggest that in addition to inhibiting acetylcholinesterase (AChE), CPF oxon may also interact direct...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-001-0309-3
更新日期:2002-01-01 00:00:00
abstract::The cytotoxicity of trivalent and pentavalent inorganic arsenic salts was determined in mouse fibroblasts in vitro. Concentrations of As (III) in the microM range led to a reduction of proliferation and viability with a concomitant increase in LDH release and stimulation of lactic acid production. Similar effects were...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00290882
更新日期:1985-08-01 00:00:00
abstract::In vitro assays are often used for the hazard characterisation of compounds, but their application for quantitative risk assessment purposes is limited. This is because in vitro assays cannot provide a complete in vivo dose-response curve from which a point of departure (PoD) for risk assessment can be derived, like t...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-013-1107-4
更新日期:2013-09-01 00:00:00
abstract::Organophosphate-induced delayed polyneuropathy (OPIDP) is initiated by inhibition/aging of more than 70-75% of neuropathy target esterase (NTE). Di-n-butyl-2,2-dichlorovinyl phosphate (DBDCVP) (1 mg/kg s.c.) inhibited 96%, 86% and 83% of NTE in brain, spinal cord and peripheral nerve, respectively, and induced a typic...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00316450
更新日期:1989-01-01 00:00:00
abstract::The effect of various hepatotoxicants on urinary taurine and urinary creatine has been studied in the rat. Several hepatotoxic agents, carbon tetrachloride, thioacetamide, galactosamine and allyl alcohol which all caused hepatic necrosis (sometimes accompanied by steatosis), resulted in a rise in urinary taurine and i...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF01974343
更新日期:1993-01-01 00:00:00
abstract::Epidemiological and experimental studies have demonstrated the atherogenic effects of environmental toxicant arsenic and fluoride. Inflammatory mechanism plays an important role in the pathogenesis of atherosclerosis. The aim of the present study is to determine the effect of chronic exposure to arsenic and fluoride a...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-012-0803-9
更新日期:2012-06-01 00:00:00
abstract::Dose-dependent excretion of p,p'DDE in rats was investigated. p,p'DDE itself was the major excreta in rats. But some o,p'isomer of DDE was detected in feces by GC-MS analysis. The excretion of p,p'DDE after a single administration was modified by its dose level. The time pattern of p,p'DDE excretion agrees well with t...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00332361
更新日期:1982-01-01 00:00:00
abstract::Certain bisphenols (BPs) have been regarded as endocrine-disrupting chemicals due to their structural similarities to bisphenol A (BPA), a well-known weak estrogenic chemical. However, very limited data are currently available on the relationship between estrogenic activity and the structure of BP analogs. Therefore, ...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-017-2011-0
更新日期:2017-12-01 00:00:00
abstract::The hepatotoxicity of acetaminophen (APAP) overdose depends on metabolic activation to a toxic reactive metabolite via hepatic mixed function oxidase. In vitro studies have indicated that APAP may also be cooxidized by prostaglandin H synthetase. The present experiments were designed to assess the possible contributio...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF01972990
更新日期:1990-01-01 00:00:00
abstract::Sunitinib (SNT) is a multi-targeted receptor tyrosine kinase inhibitor that has been approved by the FDA for cancer therapy. However, its cardiotoxicity has limited the clinical applicability with no effective therapeutic approach available. As a broadband kinase inhibitor, the function of several kinases that are ess...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-019-02448-z
更新日期:2019-06-01 00:00:00
abstract::Increasing incidence of multidrug-resistant bacteria presents an imminent risk to global health. Polymyxins are 'last-resort' antibiotics against Gram-negative 'superbugs'; however, nephrotoxicity remains a key impediment in their clinical use. Molecular mechanisms underlying this nephrotoxicity remain poorly defined....
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-018-2192-1
更新日期:2018-07-01 00:00:00