Abstract:
:The Drosophila testis is a well-established system for studying stem cell self-renewal and competition. In this tissue, the niche supports two stem cell populations, germ line stem cells (GSCs), which give rise to sperm, and somatic stem cells called cyst stem cells (CySCs), which support GSCs and their descendants. It has been established that CySCs compete with each other and with GSCs for niche access, and mutations have been identified that confer increased competitiveness to CySCs, resulting in the mutant stem cell and its descendants outcompeting wild type resident stem cells. Socs36E, which encodes a negative feedback inhibitor of the JAK/STAT pathway, was the first identified regulator of niche competition. The competitive behavior of Socs36E mutant CySCs was attributed to increased JAK/STAT signaling. Here we show that competitive behavior of Socs36E mutant CySCs is due in large part to unbridled Mitogen-Activated Protein Kinase (MAPK) signaling. In Socs36E mutant clones, MAPK activity is elevated. Furthermore, we find that clonal upregulation of MAPK in CySCs leads to their outcompetition of wild type CySCs and of GSCs, recapitulating the Socs36E mutant phenotype. Indeed, when MAPK activity is removed from Socs36E mutant clones, they lose their competitiveness but maintain self-renewal, presumably due to increased JAK/STAT signaling in these cells. Consistently, loss of JAK/STAT activity in Socs36E mutant clones severely impairs their self-renewal. Thus, our results enable the genetic separation of two essential processes that occur in stem cells. While some niche signals specify the intrinsic property of self-renewal, which is absolutely required in all stem cells for niche residence, additional signals control the ability of stem cells to compete with their neighbors. Socs36E is node through which these processes are linked, demonstrating that negative feedback inhibition integrates multiple aspects of stem cell behavior.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Amoyel M,Anderson J,Suisse A,Glasner J,Bach EAdoi
10.1371/journal.pgen.1005815subject
Has Abstractpub_date
2016-01-25 00:00:00pages
e1005815issue
1eissn
1553-7390issn
1553-7404pii
PGENETICS-D-15-02171journal_volume
12pub_type
杂志文章相关文献
PLoS Genetics文献大全abstract::Third-generation cephalosporins are a class of β-lactam antibiotics that are often used for the treatment of human infections caused by Gram-negative bacteria, especially Escherichia coli. Worryingly, the incidence of human infections caused by third-generation cephalosporin-resistant E. coli is increasing worldwide. ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004776
更新日期:2014-12-18 00:00:00
abstract::The apple is the most common and culturally important fruit crop of temperate areas. The elucidation of its origin and domestication history is therefore of great interest. The wild Central Asian species Malus sieversii has previously been identified as the main contributor to the genome of the cultivated apple (Malus...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002703
更新日期:2012-01-01 00:00:00
abstract::Genome-wide transcription start site (TSS) profiles of the enterobacteria Escherichia coli and Klebsiella pneumoniae were experimentally determined through modified 5' RACE followed by deep sequencing of intact primary mRNA. This identified 3,746 and 3,143 TSSs for E. coli and K. pneumoniae, respectively. Experimental...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002867
更新日期:2012-01-01 00:00:00
abstract::Both genetic and epigenetic alterations contribute to Facio-Scapulo-Humeral Dystrophy (FSHD), which is linked to the shortening of the array of D4Z4 repeats at the 4q35 locus. The consequence of this rearrangement remains enigmatic, but deletion of this 3.3-kb macrosatellite element might affect the expression of the ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000394
更新日期:2009-02-01 00:00:00
abstract::Auxin is a major developmental regulator in plants and the acquisition of a transcriptional response to auxin likely contributed to developmental innovations at the time of water-to-land transition. Auxin Response Factors (ARFs) Transcription Factors (TFs) that mediate auxin-dependent transcriptional changes are divid...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008400
更新日期:2019-09-25 00:00:00
abstract::To understand the genetic mechanisms leading to phenotypic differentiation, it is important to identify genomic regions under selection. We scanned the genome of two chicken lines from a single trait selection experiment, where 50 generations of selection have resulted in a 9-fold difference in body weight. Analyses o...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001188
更新日期:2010-11-04 00:00:00
abstract::The coordination of growth with nutritional status is essential for proper development and physiology. Nutritional information is mostly perceived by peripheral organs before being relayed to the brain, which modulates physiological responses. Hormonal signaling ensures this organ-to-organ communication, and the failu...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005209
更新日期:2015-05-28 00:00:00
abstract::Plant microRNAs (miRNAs) are critical regulators of gene expression, however little attention has been given to the principles governing miRNA silencing efficacy. Here, we utilize the highly conserved Arabidopsis miR159-MYB33/MYB65 regulatory module to explore these principles. Firstly, we show that perfect central co...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004232
更新日期:2014-03-13 00:00:00
abstract::N-methyl-D-aspartate receptors (NMDARs), ligand-gated ionotropic glutamate receptors, play key roles in normal brain development and various neurological disorders. Here we use standing variation data from the human population to assess which protein domains within NMDAR GluN1, GluN2A and GluN2B subunits show the stro...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006536
更新日期:2017-01-17 00:00:00
abstract::Considerable evidence now supports the idea that the moderate telomere lengthening produced by recombinational telomere elongation (RTE) in a Kluyveromyces lactis telomerase deletion mutant occurs through a roll-and-spread mechanism. However, it is unclear whether this mechanism can account for other forms of RTE that...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003017
更新日期:2012-01-01 00:00:00
abstract::Respiratory infectious diseases are the third cause of worldwide death. The nasopharynx is the portal of entry and the ecological niche of many microorganisms, of which some are pathogenic to humans, such as Neisseria meningitidis and Moraxella catarrhalis. These microbes possess several surface structures that intera...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005338
更新日期:2015-07-10 00:00:00
abstract::We report association mapping of a locus on bovine chromosome 3 that underlies a Mendelian form of stunted growth in Belgian Blue Cattle (BBC). By resequencing positional candidates, we identify the causative c124-2A>G splice variant in intron 1 of the RNF11 gene, for which all affected animals are homozygous. We make...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002581
更新日期:2012-01-01 00:00:00
abstract::Adipose tissue lipolysis occurs during the development of heart failure as a consequence of chronic adrenergic stimulation. However, the impact of enhanced adipose triacylglycerol hydrolysis mediated by adipose triglyceride lipase (ATGL) on cardiac function is unclear. To investigate the role of adipose tissue lipolys...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007171
更新日期:2018-01-10 00:00:00
abstract::NF-κB is a key transcription factor regulating the expression of inflammatory responsive genes. How NF-κB binds to naked DNA templates is well documented, but how it interacts with chromatin is far from being clear. Here we used a combination of UV laser footprinting, hydroxyl footprinting and electrophoretic mobility...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003830
更新日期:2013-01-01 00:00:00
abstract::Cis-regulation plays an essential role in the control of gene expression, and is particularly complex and poorly understood for developmental genes, which are subject to multiple levels of modulation. In this study, we performed a global analysis of the cis-acting elements involved in the control of the zebrafish deve...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007581
更新日期:2018-08-06 00:00:00
abstract::Innate immunity in Caenorhabditis elegans requires a conserved PMK-1 p38 mitogen-activated protein kinase (MAPK) pathway that regulates the basal and pathogen-induced expression of immune effectors. The mechanisms by which PMK-1 p38 MAPK regulates the transcriptional activation of the C. elegans immune response have n...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000892
更新日期:2010-04-01 00:00:00
abstract::The mechanism underlying immune system recognition of different types of pathogens has been extensively studied over the past few decades; however, the mechanism by which healthy self-tissue evades an attack by its own immune system is less well-understood. Here, we established an autoimmune model of melanotic mass fo...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004683
更新日期:2014-10-16 00:00:00
abstract::Fragile X syndrome, a common form of inherited mental retardation, is caused by the loss of fragile X mental retardation protein (FMRP). We have previously demonstrated that dFmr1, the Drosophila ortholog of the fragile X mental retardation 1 gene, plays a role in the proper maintenance of germline stem cells in Droso...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000444
更新日期:2009-04-01 00:00:00
abstract::Smc5/6, a member of the conserved SMC family of complexes, is essential for growth in most organisms. Its exact functions in a mitotic cell cycle are controversial, as chronic Smc5/6 loss-of-function alleles produce varying phenotypes. To circumvent this issue, we acutely depleted Smc5/6 in budding yeast and determine...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007129
更新日期:2018-01-23 00:00:00
abstract::The evolutionary divergence of mitochondrial ribosomes from their bacterial and cytoplasmic ancestors has resulted in reduced RNA content and the acquisition of mitochondria-specific proteins. The mitochondrial ribosomal protein of the small subunit 34 (MRPS34) is a mitochondria-specific ribosomal protein found only i...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005089
更新日期:2015-03-27 00:00:00
abstract::[This corrects the article DOI: 10.1371/journal.pgen.1006396.]. ...
journal_title:PLoS genetics
pub_type: 杂志文章,已发布勘误
doi:10.1371/journal.pgen.1007639
更新日期:2018-08-30 00:00:00
abstract::Many diseases are differentially distributed among human populations. Differential selection on genetic variants in ancestral environments that coincidentally predispose to disease can be an underlying cause of these unequal prevalence patterns. Selected genes may be pleiotropic, affecting multiple phenotypes and resu...
journal_title:PLoS genetics
pub_type: 杂志文章,评审
doi:10.1371/journal.pgen.1005568
更新日期:2015-11-05 00:00:00
abstract::Hormone-dependent activation of enhancers includes histone hyperacetylation and mediator recruitment. Histone hyperacetylation is mostly explained by a bimodal switch model, where histone deacetylases (HDACs) disassociate from chromatin, and histone acetyl transferases (HATs) are recruited. This model builds on decade...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008770
更新日期:2020-05-26 00:00:00
abstract::The Caenorhabditis elegans class A and B synthetic multivulva (synMuv) genes redundantly antagonize an EGF/Ras pathway to prevent ectopic vulval induction. We identify a class A synMuv mutation in the promoter of the lin-3 EGF gene, establishing that lin-3 is the key biological target of the class A synMuv genes in vu...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002418
更新日期:2011-12-01 00:00:00
abstract::Centrioles are microtubule-based organelles important for the formation of cilia, flagella and centrosomes. Despite progress in understanding the underlying assembly mechanisms, how centriole integrity is ensured is incompletely understood, including in sperm cells, where such integrity is particularly critical. We id...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004777
更新日期:2014-11-20 00:00:00
abstract::The evolution of insecticide resistance is a global constraint to agricultural production. Spinosad is a new, low-environmental-risk insecticide that primarily targets nicotinic acetylcholine receptors (nAChR) and is effective against a wide range of pest species. However, after only a few years of application, field ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000802
更新日期:2010-01-01 00:00:00
abstract::We carried out an admixture analysis of a sample comprising 1,019 individuals from all the provinces of Cuba. We used a panel of 128 autosomal Ancestry Informative Markers (AIMs) to estimate the admixture proportions. We also characterized a number of haplogroup diagnostic markers in the mtDNA and Y-chromosome in orde...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004488
更新日期:2014-07-24 00:00:00
abstract::Genomic concepts are based on the assumption that phenotypes arise from the expression of genetic variants. However, the presence of non-Mendelian inheritance patterns provides a direct challenge to this view and suggests an important role for alternative mechanisms of gene regulation and inheritance. Over the past fe...
journal_title:PLoS genetics
pub_type: 杂志文章,评审
doi:10.1371/journal.pgen.1004296
更新日期:2014-04-17 00:00:00
abstract::Recent studies of the HapMap lymphoblastoid cell lines have identified large numbers of quantitative trait loci for gene expression (eQTLs). Reanalyzing these data using a novel Bayesian hierarchical model, we were able to create a surprisingly high-resolution map of the typical locations of sites that affect mRNA lev...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000214
更新日期:2008-10-01 00:00:00
abstract::Insertions and deletions (indels) are a major source of genetic variation within species and may result in functional changes to coding or regulatory sequences. In this study we report that an indel polymorphism in the 3' untranslated region (UTR) of the metallothionein gene MtnA is associated with gene expression var...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005987
更新日期:2016-04-27 00:00:00