Abstract：
INTRODUCTION:Quantification of collagen degradation is an important parameter to evaluate dentin caries progression or the efficacy of caries prevention aid. The aim of this study was to validate the simple light microscopic technique (LM) to evaluate collagen degradation by comparing with hydroxyproline assay technique (HPN). MATERIALS AND METHODS:Bovine root dentin blocks were embedded in acrylic resin, polished and covered with nail varnish except a 1.5 × 2.5mm window. The specimens were demineralized in acetate buffer (pH 4.3) for 3 days to create incipient lesions and were exposed to collagenase enzyme for 6, 9 and 16 h. The specimens were sectioned into thin sections (200-220 μm) to measure the degraded depth of collagen matrix by LM. The enzyme solutions were allocated to HPN assay using the simplified chloramines-T method. Correlation between LM and HPN was evaluated by Pearson correlation analysis. Anti-collagen degradation efficacy of 0.12% chlorhexidine (CHX) was evaluated by LM. RESULT:The depths of the degraded collagen and amount of hydroxyproline in 3 exposure periods were 27.8 ± 3.8 μm and 28.7 ± 4.2 μg for 6h, 48.1 ± 8.6 μm and 45.3 ± 6.1 μg for 9h, and 74.2 ± 9.7 μm and 71.3 ± 8.0 μg for 16 h, respectively. A significantly positive correlation (r=0.94, CI: 0.88-0.97, p<0.0001) was observed between LM and HPN and incubation time showed a linear correlation with amount of collagen degradation (R(2)=0.92). The CHX group (28.6 ± 3.3 μm) showed significantly lower collagen degradation than that of control group (53.1 ± 7.8 μm: p<0.01). CONCLUSION:The LM might be a reliable and simplified method to evaluate collagen degradation.
journal_name
J Dentjournal_title
Journal of dentistryauthors
Islam MS,Khunkar SJ,Nakashima S,Sadr A,Nikaido T,Tagami Jdoi
10.1016/j.jdent.2016.01.001subject
Has Abstractpub_date
2016-04-01 00:00:00pages
94-7eissn
0300-5712issn
1879-176Xpii
S0300-5712(16)30001-Xjournal_volume
47pub_type
杂志文章abstract：OBJECTIVES:Inhibition of hybrid layer degradation, for example via inhibition of matrix-metallo-proteinases (MMP) could reduce risk of retention loss and failure of adhesively placed restorations. This systematic review investigated such inhibitory pretreatment qualitatively and via meta- and trial-sequential-analysis....
journal_title：Journal of dentistry
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.jdent.2016.04.007
更新日期：2016-06-01 00:00:00
abstract：:The complexity of the oral environment, and ethical problems associated with studies of oral diseases in humans inevitably directed the attention to development of laboratory models, that simulate the human oral microcosm. These developments and in particular the in vitro 'artificial mouth' systems have progressed fro...
journal_title：Journal of dentistry
pub_type： 杂志文章,评审
doi：10.1016/s0300-5712(03)00009-5
更新日期：2003-03-01 00:00:00
abstract：OBJECTIVES:The purpose of the study was to evaluate the intra- and inter-examiner variability of a panel of examiners using a 'glance and grade' marking system when assessing Class II preparations. METHODS:The examination panel consisted of five university staff with varying clinical and teaching experience. The panel...
journal_title：Journal of dentistry
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doi：10.1016/s0300-5712(97)00033-x
更新日期：1998-11-01 00:00:00
abstract：OBJECTIVE:This randomized controlled trial compared the survival of glass fibre and cast metal dental posts used to restore endodontically treated teeth with no remaining coronal wall. METHODS:Fifty-four participants (45 women) and 72 teeth were evaluated during a follow-up period of up to 3 years. Teeth were randomly...
journal_title：Journal of dentistry
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jdent.2014.02.003
更新日期：2014-05-01 00:00:00
abstract：OBJECTIVES:To assess the cuspal deflection and cervical microleakage of standardised Class II cavities incrementally filled with a dimethacrylate RBC or bulk-fill flowable RBC bases. METHODS:Twenty-four sound upper premolar teeth with Class II cavities were allocated to three groups (n=8). Restoration of the teeth inv...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2012.02.015
更新日期：2012-06-01 00:00:00
abstract：OBJECTIVES:Secondary caries at the tooth-restoration margins is a primary reason for restoration failure. Cracks at the margins lead to leakage which can trap bacteria, producing acids to cause caries. To date, there has been no report on developing an adhesive resin that has self-healing, antibacterial and remineraliz...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2018.05.009
更新日期：2018-08-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to investigate the kinetics of protein interactions with a novel hydroxyapatite-polylactide (HA-PLA) composite membrane material. METHODS:Trilayer PLA and HA-PLA composite membranes reinforced with PLA fibres were used to absorb and release protein which was measured by a BioRad as...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2009.06.014
更新日期：2009-11-01 00:00:00
abstract：OBJECTIVE:to assess the expectations, perceptions and performance of different operators with varying backgrounds and training in the use of full-arch intraoral scanning (IOS). METHODS AND MATERIALS:Dentists (DENT), postgraduate dental students (DPG), and student prosthetists (PROS) were invited to join an IOS trainin...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2020.103572
更新日期：2020-12-29 00:00:00
abstract：OBJECTIVE:Silver compounds have been used for their medical properties for centuries and in dentistry for more than a century. The aim of this review is to examine the evidence supporting the therapeutic use of silver in dentistry and the outcomes from the clinical trials, as well as mode of action and biocompatibility...
journal_title：Journal of dentistry
pub_type： 杂志文章,评审
doi：10.1016/j.jdent.2012.03.009
更新日期：2012-07-01 00:00:00
abstract：OBJECTIVE:This study investigated the mechanism of action at the interface between a commercially available Y-TZP and its veneering ceramic after final firing. Particular attention was paid, from a microstructural point of view, to evaluating the effects of different surface treatments carried out on the zirconia. MET...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2014.07.019
更新日期：2014-11-01 00:00:00
abstract：OBJECTIVES:To investigate the effectiveness of an indicator gel in revealing exposed dentine. METHODS:Teeth prepared into dentine and then stained with the indicator gel were sectioned for histological examination. RESULTS:The indicator gel was found to stain dentine and thin layers of enamel. CONCLUSION:The gel is ...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/0300-5712(94)00010-d
更新日期：1995-12-01 00:00:00
abstract：:The purpose of this study was to evaluate the effect of a coaxial carbon dioxide/neodymium:yttrium aluminium garnet laser beam on enamel surface roughness and the dental pulps of mongrel dogs. In four dogs, four maxillary left posterior teeth were irradiated at 16 cm source-tooth distances. Two teeth were irradiated w...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/0300-5712(91)90095-g
更新日期：1991-04-01 00:00:00
abstract：OBJECTIVES:The purpose of this study was to evaluate the in vitro effectiveness of a new bioglass-containing and two commercial desensitising toothpastes on dentinal tubule occlusion after citric acid challenge or artificial saliva (AS) immersion. METHODS:One hundred dentin discs from human third molars were used. Spe...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2010.01.007
更新日期：2010-05-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to assess the prevalence of direct restorations in posterior teeth in children aged 12, from a birth cohort, and to test the association between the quality of the restorations and individual variables experienced in the life cycle. METHODS:All live-born children in Pelotas, in 200...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2019.103183
更新日期：2019-10-01 00:00:00
abstract：OBJECTIVES:To integrate items from two widely used oral health-related quality of life (OHRQoL) questionnaires, the General Oral Health Assessment Index (GOHAI) and the Oral Impacts on Daily Performances (OIDP), as well as culturally-specific items of the Oral Health Impact Profile (OHIP) into a four-dimensional OHRQoL...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2016.06.006
更新日期：2016-10-01 00:00:00
abstract：OBJECTIVES:Teeth in a patient's mouth in a dental office, or in the natural environment, represent very complex stimuli for the human color vision system. Predicting their perceived color is a daunting task at best. Colorimetry is designed mainly for the evaluation of uniform, flat, opaque, materials of fairly large si...
journal_title：Journal of dentistry
pub_type： 杂志文章,评审
doi：10.1016/j.jdent.2010.05.008
更新日期：2010-01-01 00:00:00
abstract：OBJECTIVE:Sodium hexametaphosphate (SHMP) is a widely used industrial preservative commonly found in children's drinks. In this paper we examined the effect of SHMP incorporated into children's drinks on acid production by the oral biofilm by monitoring salivary concentrations of lactic acid. MATERIALS AND METHOD:Twel...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2006.08.003
更新日期：2007-03-01 00:00:00
abstract：OBJECTIVES:Small and hydrophilic monomers as HEMA and TEGDMA can easily penetrate human tissues. For biocompatibility issues it is therefore better to avoid such monomers in dental adhesive formulations. The purpose of this study was (1) to determine the micro-tensile bond strength (muTBS) to enamel/dentin of a HEMA/TE...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2008.05.012
更新日期：2008-10-01 00:00:00
abstract：OBJECTIVE:This study evaluated the effects of surface preparation and the application time of adhesives on the resin-dentine bond strengths with universal adhesives. METHODS:Sixty molars were cut to exposed mid-coronal dentine and divided into 12 groups (n=5) based on three factors; (1) adhesive: G-Premio Bond (GP, GC...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2016.02.001
更新日期：2016-04-01 00:00:00
abstract：:An even fitting surface is essential to obtain and maintain a healthy oral mucosa in denture wearers. The present study was designed to clarify the relationship between the surface roughness of oral mucosa and its reproduction in the corresponding fitting surface of acrylic dentures. A silicone elastomer/epoxy replica...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/0300-5712(94)90201-1
更新日期：1994-06-01 00:00:00
abstract：OBJECTIVES:The aim of this double-blind clinical trial was to assess the longevity of repairs to localized clinical defects in composite resin restorations that were initially planned to be treated with a restoration replacement. METHODS:Twenty-eight patients aged 18-80 years old with 50 composite resin restorations (...
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pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jdent.2014.05.015
更新日期：2015-02-01 00:00:00
abstract：:The Annual Conference of the British Association of Teachers of Conservative Dentistry (BATCD) was held in Sheffield on 16 and 17 September 1993. The main programme addressed educational issues in three areas: the undergraduate/vocational-trainee interface, the teaching of implants in the undergraduate curriculum, and...
journal_title：Journal of dentistry
pub_type：
doi：10.1016/0300-5712(94)90069-8
更新日期：1994-10-01 00:00:00
abstract：OBJECTIVE:This study compared the antibacterial activities of two dentin bonding systems (ABF, Kuraray and Reactmer Bond, Shofu) by a conventional agar well technique and a newly designed in vitro test using tooth model. METHODS:In the agar well technique, the test materials were filled in the wells of Muller Hinton a...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/s0300-5712(03)00006-x
更新日期：2003-02-01 00:00:00
abstract：OBJECTIVES:To determine the longevity of heat-pressed glass ceramic inlays and onlays luted with solely light-curing composite resin. METHODS:The records of patients who had received a ceramic inlay or onlay at the Heidelberg University Department of Conservative Dentistry from 1993 to 2002 were evaluated. The mean ob...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2004.10.026
更新日期：2005-05-01 00:00:00
abstract：:Some laboratory tests of impression material accuracy mimic the clinical situation (simulatory) while others attempt to quantify a material's individual properties. This review concentrates on simulatory testing and aims to give a classification of the numerous tests available. Measurements can be made of the impressi...
journal_title：Journal of dentistry
pub_type： 杂志文章,评审
doi：10.1016/0300-5712(92)90117-u
更新日期：1992-04-01 00:00:00
abstract：INTRODUCTION:C. tropicalis and C. krusei have emerged as virulent species causing oral infections. Both have developed resistance to commonly prescribed azole antifungal agents. OBJECTIVE:The study aimed to determine the effect of mouth rinses containing chlorhexidine digluconate (CHX), cetylpyridinium chloride (CPC) ...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2012.04.003
更新日期：2012-07-01 00:00:00
abstract：OBJECTIVES:Effective delivery of active agents to dental hard tissues is critical to product performance and pertinent to biofilm control, caries, erosion, hypersensitivity and tooth bleaching. The aim of this study was to investigate in vitro the bioadhesion and retention behaviour of non-aqueous delivery systems (NAD...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2010.06.005
更新日期：2010-09-01 00:00:00
abstract：:The effects of sample thickness on the in vitro bond strength of two light-activated glass polyalkenoate cements to dentine have been investigated. The buccal and/or lingual surfaces of non-carious human third molar teeth were used for bonding. The tensile bond strengths to dentine of samples of the cements of 0.5 mm,...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/0300-5712(94)90123-6
更新日期：1994-08-01 00:00:00
abstract：OBJECTIVE:To explore the association between tooth wear and quality of life among adults in the United Kingdom, independently of sociodemographic factors and other common oral conditions. METHODS:We used data from 5654 dentate adults who participated in the 2009 Adult Dental Health Survey. Tooth wear was assessed duri...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2016.09.013
更新日期：2016-12-01 00:00:00
abstract：OBJECTIVES:Wear of dental hard tissues, e.g. dental erosion, is reported to be a growing problem. A non-destructive measurement of enamel layer thickness would provide the opportunity for both early diagnosis, and longitudinal measurement of progressive enamel loss. It was the aim of this study to investigate the poten...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/s0300-5712(99)00063-9
更新日期：2000-03-01 00:00:00
