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Variability in follicular fluid high density lipoprotein particle components measured in ipsilateral follicles.

Abstract：

PURPOSE:The purpose of this study was to examine the biological variability of follicular fluid (FF) high density lipoprotein (HDL) particle components measured in ipsilateral ovarian follicles. METHODS:We collected FF from two ipsilateral follicles among six women undergoing in vitro fertilization (IVF). We measured concentrations of 19 FF HDL particle components, including HDL cholesterol, free cholesterol, four cholesteryl esters, phospholipids, triglycerides, paraoxonase and arylesterase activities, apolipoproteins A-1 and A-2 (ApoA-1 and ApoA-2), and seven lipophilic micronutrients, by automated analysis and with high-performance liquid chromatography. We assessed biological variability using two-stage nested analysis of variance and compared values with those previously published for contralateral follicles. RESULTS:For most FF HDL analytes, there was little variability between follicles relative to the variability between women (i.e., %σ(2) F:%σ(2) B <0.5). Intraclass correlation coefficients were >0.80 for HDL cholesterol (0.82), phospholipids (0.89), paraoxonase (0.96), and arylesterase (0.91) activities, ApoA-1 (0.89), and ApoA-2 (0.90), and single specimen collections were required to estimate the subject-specific mean, demonstrating sufficient reliability for use as biomarkers of the follicular microenvironment in epidemiologic and clinical studies. CONCLUSIONS:These preliminary results raise the possibility for tighter regulation of HDL in follicles within the same ovary vs. between ovaries. Thus, collection of a single FF specimen may be sufficient to estimate HDL particle components concentrations within a single ovary. However, our results should be interpreted with caution as the analysis was based on a small sample.

journal_name

J Assist Reprod Genet

journal_title

Journal of assisted reproduction and genetics

authors

Kim K,Bloom MS,Fujimoto VY,Bell EM,Yucel RM,Browne RW

doi

10.1007/s10815-016-0648-x

subject

Has Abstract

pub_date

2016-03-01 00:00:00

pages

423-430

issue

eissn

1058-0468

issn

1573-7330

pii

10.1007/s10815-016-0648-x

journal_volume

pub_type

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Variability in follicular fluid high density lipoprotein particle components measured in ipsilateral follicles.