Downregulation of miR-210 protected bupivacaine-induced neurotoxicity in dorsal root ganglion.

Abstract:

:Local anesthetic may cause neurotoxicity in developing neurons. In this study, we examined the molecular mechanisms of microRNA-210 (miR-210) in regulating bupivacaine-induced dorsal root ganglia (DRG) neurotoxicity in vitro. Young mouse (P30) DRG explants were cultured in vitro and treated with 5 mM bupivacaine to induce neurotoxicity. QRT-PCR was used to evaluate the expression profiles of miRNAs within 24 h after bupivacaine treatment. MiR-210 was downregulated in DRG, and its effects on bupivacaine-induced neurotoxicity were evaluated by apoptosis and neurite growth assays, respectively. Putative downstream target of miR-210 in DRG, BDNF, was evaluated by dual-luciferase assay, qRT-PCR, and western blot, respectively. BDNF was then knocked down by siRNA to assess its associated effects in regulating DRG neurotoxicity. Within the initial 24 h after bupivacaine treatment, various patterns of miRNA expression were observed, whereas miR-210 was constantly upregulated. Application of miR-210 inhibitor efficiently downregulated endogenous miR-210, protected apoptosis and neurite retraction in bupivacaine damaged DRG neurons. Using dual-luciferase assay, qRT-PCR, and western blot, BDNF was confirmed to the downstream target of miR-210 in DRG. SiRNA-mediated BDNF downregulation reversed the effect of miR-210 downregulation in DRG neurotoxicity. MiR-210, through the regulation of BDNF, plays important role in anesthetics-induced DRG neurotoxicity.

journal_name

Exp Brain Res

authors

Wang Y,Ni H,Zhang W,Wang X,Zhang H

doi

10.1007/s00221-015-4513-4

subject

Has Abstract

pub_date

2016-04-01 00:00:00

pages

1057-65

issue

4

eissn

0014-4819

issn

1432-1106

pii

10.1007/s00221-015-4513-4

journal_volume

234

pub_type

杂志文章
  • Electrophysiological evidence for the dendritic localization of a calcium conductance in guinea-pig substantia nigra neurones in vitro.

    abstract::Within the substantia nigra, anatomical, neurochemical and pharmacological findings strongly suggest that transmitter and protein are secreted from the dendrites of nigrostriatal neurones. This phenomenon may underlie a non classical modulatory cellular mechanism. Two conductances are generated in nigrostriatal neuron...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/BF00248875

    authors: Harris NC,Ramsay S,Kelion A,Greenfield SA

    更新日期:1989-01-01 00:00:00

  • Binocular phase specificity of striate cortical neurones.

    abstract::Sensitivity to binocular positional mismatch was evaluated in cat striate cortical neurones, for paired, dichoptically presented, moving sine-wave gratings whose interocular positional phase relationship was varied. Spatial frequency, orientation and velocity were optimized for each neurone. Binocular responses for ea...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/BF00227086

    authors: Hammond P

    更新日期:1991-01-01 00:00:00

  • Adaptation and habituation characteristics of tectal neurons in the pigeon.

    abstract::The adaptation and habituation characteristics produced by the repeated presentation of a moving visual stimulus were studied in single cells in the pigeon's optic tectum. Units located near the surface in the stratum opticum showed little adaptation. However, cells located in deeper layers exhibited varying rates and...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/BF00235509

    authors: Woods EJ,Frost BJ

    更新日期:1977-03-30 00:00:00

  • Direction and amplitude precuing has no effect on automatic posture responses.

    abstract::Automatic postural responses of leg muscles to the sudden displacement of standing support were investigated under four different conditions of information given to subjects in advance. Results from three groups of subjects were compared: 6 normal subjects, 10 patients with cerebellar disease, and 9 patients with Park...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/BF00231777

    authors: Diener HC,Horak F,Stelmach G,Guschlbauer B,Dichgans J

    更新日期:1991-01-01 00:00:00

  • Role of the rostral ventrolateral medulla (RVLM) in the patterning of vestibular system influences on sympathetic nervous system outflow to the upper and lower body.

    abstract::Research on animal models as well as human subjects has demonstrated that the vestibular system contributes to regulating the distribution of blood in the body through effects on the sympathetic nervous system. Elimination of vestibular inputs results in increased blood flow to the hindlimbs during vestibular stimulat...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-011-2550-1

    authors: Sugiyama Y,Suzuki T,Yates BJ

    更新日期:2011-05-01 00:00:00

  • Head movement propensity.

    abstract::In studies of human eye-head coordinated saccadic gaze shifts, different laboratories have found greatly different magnitudes of head movements for a given amplitude of gaze shift (head movement gain). The present study was conducted to examine why, and to quantify volitional head movements. Fixation/target lights wer...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/BF00230391

    authors: Fuller JH

    更新日期:1992-01-01 00:00:00

  • Floccular lesions abolish adaptive control of post-saccadic ocular drift in primates.

    abstract::After several days of exposure to optically-imposed post-saccadic retinal slip, the saccades of normal monkeys acquire an exponential ocular drift. This drift is in the direction of the imposed image motion, and persists in the dark. It has been argued that these changes result from the operation of a visually mediate...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/BF00340497

    authors: Optican LM,Zee DS,Miles FA

    更新日期:1986-01-01 00:00:00

  • The quantitative use of velocity information in fast interception.

    abstract::We ask whether a target's velocity is considered when planning a fast interceptive action. Human volunteers hit targets that could move at different velocities from across a tilted screen (the hand starting 40 cm away from the screen). We examined how the direction in which the hand initially moved depended on the tar...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-004-1832-2

    authors: de Lussanet MH,Smeets JB,Brenner E

    更新日期:2004-07-01 00:00:00

  • Grasping the Müller-Lyer illusion: not a change in perceived length.

    abstract::Peak grip aperture has often been used to quantify the influence of illusions on judgments of size for action. However, a larger peak grip aperture need not mean that the object looks larger. It could also mean that it was grasped more carefully. These two possibilities can be distinguished on the basis of the velocit...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-006-0744-8

    authors: Biegstraaten M,de Grave DD,Brenner E,Smeets JB

    更新日期:2007-01-01 00:00:00

  • Inferring the future target trajectory from visual context: is visual background structure used for anticipatory smooth pursuit?

    abstract::Anticipatory pursuit is not exclusively based on the recent history of target motion ("temporal anticipation"): it can also use static visual cues ("non-temporal anticipation"). Large non-temporal anticipatory changes of the direction of smooth pursuit are observed when the future trajectory of the target can be infer...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-009-1840-3

    authors: Eggert T,Ladda J,Straube A

    更新日期:2009-06-01 00:00:00

  • Walking performance and its recovery in chronic stroke in relation to extent of lesion overlap with the descending motor tract.

    abstract::We investigated the association between the degree of lesion overlap with the corticospinal tract and walking performance before and after 4-weeks of partial body weight support (PBWS) treadmill training in 18 individuals (ten male, eight female) with a mean age 59 +/- 13 years (mean +/- SD), range 32-74 years, who we...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-007-1237-0

    authors: Dawes H,Enzinger C,Johansen-Berg H,Bogdanovic M,Guy C,Collett J,Izadi H,Stagg C,Wade D,Matthews PM

    更新日期:2008-03-01 00:00:00

  • Combined effects of auditory and visual cues on the perception of vection.

    abstract::Vection is the illusion of self-motion in the absence of real physical movement. The aim of the present study was to analyze how multisensory inputs (visual and auditory) contribute to the perception of vection. Participants were seated in a stationary position in front of a large, curved projection display and were e...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-013-3793-9

    authors: Keshavarz B,Hettinger LJ,Vena D,Campos JL

    更新日期:2014-03-01 00:00:00

  • Sex differences in the acquisition of complex skilled movements.

    abstract::The praxis system comprises a network of brain regions dedicated to complex skilled movements. Following suggestions of a female advantage on learned movements (Chipman and Hampson in Neuropsychologia 44(12):2315-2329, 2006), we investigated how males and females acquire skilled movements. Subjects viewed and imitated...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-010-2351-y

    authors: Cohen NR,Pomplun M,Gold BJ,Sekuler R

    更新日期:2010-08-01 00:00:00

  • Modulatory effects of high-frequency repetitive transcranial magnetic stimulation on the ipsilateral silent period.

    abstract::In healthy subjects, suprathreshold repetitive transcranial magnetic stimulation (rTMS) at frequencies >2 Hz prolongs the cortical silent period (CSP) over the course of the train. This progressive lengthening probably reflects temporal summation of the inhibitory interneurons in the stimulated primary motor cortex (M...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-005-0296-3

    authors: Cincotta M,Giovannelli F,Borgheresi A,Balestrieri F,Zaccara G,Inghilleri M,Berardelli A

    更新日期:2006-06-01 00:00:00

  • Anticipatory postural adjustments stabilise the whole upper-limb prior to a gentle index finger tap.

    abstract::Little is known about anticipatory postural adjustments (APAs) developing when body segments of tiny mass are moved. Thus, APAs in the human upper-limb were investigated during a gentle and small index finger tap (35 mm stroke in 50 ms). This task was fulfilled by ten subjects either with prone or supine hand. EMG was...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-008-1668-2

    authors: Caronni A,Cavallari P

    更新日期:2009-03-01 00:00:00

  • Evidence of parasympathetic postganglionic neurons in the rat hypoglossal nerve trunk.

    abstract::Previous studies have indicated that the geniohyoid (GH) muscle is innervated by efferent axons from both the hypoglossal nerve (CN XII) and ansa cervicalis. To clarify the physiological significance of this dual innervation of the GH muscle, we examined properties of efferent innervations in rat GH muscle using elect...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-003-1529-y

    authors: Yasuda K,Mori R,Tanaka M,Nakayama Y,Tanaka S,Kumai T,Matsuhashi H,Kondo E,Yamaoka M,Furusawa K

    更新日期:2003-12-01 00:00:00

  • Naturalistic arm movements during obstacle avoidance in 3D and the identification of movement primitives.

    abstract::By studying human movement in the laboratory, a number of regularities and invariants such as planarity and the principle of isochrony have been discovered. The theoretical idea has gained traction that movement may be generated from a limited set of movement primitives that would encode these invariants. In this stud...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-012-3205-6

    authors: Grimme B,Lipinski J,Schöner G

    更新日期:2012-10-01 00:00:00

  • Facilitation of learning after lesions of the tuberomammillary nucleus region in adult and aged rats.

    abstract::The tuberomammillary nucleus (TM) located in the posterior part of the hypothalamus is the main source of neuronal histamine in the central nervous system. Recent work from our laboratories has indicated an involvement of the TM region in neuronal plasticity and reinforcement processes. In the present study, we invest...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s002210050301

    authors: Frisch C,Hasenöhrl RU,Haas HL,Weiler HT,Steinbusch HW,Huston JP

    更新日期:1998-02-01 00:00:00

  • Electrical activation of the human vestibulo-sympathetic reflex.

    abstract::Muscle sympathetic nerve activity (MSNA) is modulated on a beat-to-beat basis by the baroreflex. Vestibular input from the otolith organs also modulates MSNA, but characteristics of the vestibulo-sympathetic reflex (VSR) are largely unknown. The purpose of this study was to elicit the VSR with electrical stimulation t...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-005-0266-9

    authors: Voustianiouk A,Kaufmann H,Diedrich A,Raphan T,Biaggioni I,Macdougall H,Ogorodnikov D,Cohen B

    更新日期:2006-05-01 00:00:00

  • Performance of discrete, reciprocal, and cyclic movements of the ipsilesional upper limb in individuals after stroke.

    abstract::Aiming movements of the upper limbs can be classified either as discrete, or reciprocal, or cyclic. The control of these movements after a stroke can be affected. The aim of this experimental, cross-sectional study was to characterize the performance of these movements after the right and left hemisphere chronic strok...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-020-05897-6

    authors: Carvalho DB,Freitas SMSF,Alencar FAD,Silva ML,Alouche SR

    更新日期:2020-10-01 00:00:00

  • Influence of predictability on control of extra-retinal components of smooth pursuit during prolonged 2D tracking.

    abstract::We compared pursuit responses to 2D target motion in three separate conditions: predictable, randomised and randomised with timing cues. The target moved on a continuous quadrilateral path in which right-angle direction changes allowed anticipatory eye acceleration and deceleration in orthogonal axes to be assessed. R...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-014-4164-x

    authors: Barnes G,Collins S

    更新日期:2015-03-01 00:00:00

  • Transplantation of fetal lateral geniculate nucleus to the occipital cortex: connectivity with host's area 17.

    abstract::The developing lateral geniculate complex was excised from fetal albino rats at 18 days of gestation and implanted into the occipital cortex of host animals at 5 days of postnatal age. Groups of host animals were sacrificed at 10, 20 and 30 days following this procedure. The transplant tissue of selected animals was s...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/BF00235864

    authors: Matthews MA

    更新日期:1985-01-01 00:00:00

  • Comparing kinematic changes between a finger-tapping task and unconstrained finger flexion-extension task in patients with Parkinson's disease.

    abstract::Repetitive finger tapping is a well-established clinical test for the evaluation of parkinsonian bradykinesia, but few studies have investigated other finger movement modalities. We compared the kinematic changes (movement rate and amplitude) and response to levodopa during a conventional index finger-thumb-tapping ta...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-013-3491-7

    authors: Teo WP,Rodrigues JP,Mastaglia FL,Thickbroom GW

    更新日期:2013-06-01 00:00:00

  • One hour of tongue-task training is associated with plasticity in corticomotor control of the human tongue musculature.

    abstract::Corticomotor control of the human tongue has been reported to undergo neuroplastic changes following several days of training in a tongue-protrusion task. The aims of the present study were to determine if a 1 h tongue-task training is sufficient to induce signs of neuroplastic changes in the corticomotor pathways, an...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-006-0380-3

    authors: Svensson P,Romaniello A,Wang K,Arendt-Nielsen L,Sessle BJ

    更新日期:2006-08-01 00:00:00

  • Properties of a new vestibulospinal projection, the caudal vestibulospinal tract.

    abstract::Neurons in the caudal portions of the medial and descending vestibular nuclei and in vestibular cell group f that project to the cervical or lumbar spinal cord were located by antidromic spinal stimulation. These caudal vestibulospinal tract (CVST) neurons have a median conduction velocity of 12 m/sec, which is well b...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/BF00239733

    authors: Peterson BW,Maunz RA,Fukushima K

    更新日期:1978-06-19 00:00:00

  • Understanding the contribution of binocular vision to the control of adaptive locomotion.

    abstract::Abstract. Although the contribution of binocular vision to reach-to-grasp movements has been extensively studied, it has been largely ignored in locomotion. The aim of these studies was to explore the role of binocular vision during the approach phase and step over the obstacle and the contribution of head movements t...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-001-0948-x

    authors: Patla AE,Niechwiej E,Racco V,Goodale MA

    更新日期:2002-02-01 00:00:00

  • Examining the influence of 'noise' on judgements of spatial extent.

    abstract::The line bisection task--commonly used as a clinical measure of unilateral neglect--requires participants to place a mark on a horizontal line to indicate where they think centre is. In general, results suggest that the allocation of attention mediates bisection. In addition, previous research shows that participants ...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-010-2449-2

    authors: Valadao DF,Hurwitz M,Danckert J

    更新日期:2010-12-01 00:00:00

  • Interindividual variability and age-dependency of motor cortical plasticity induced by paired associative stimulation.

    abstract::Paired associative stimulation (PAS) can increase motor cortical excitability, possibly by long-term potentiation (LTP)-like mechanisms. As the capability of the cortex for plasticity decreases with age, we were interested here in testing interindividual variability and age-dependency of the PAS effect. Motor-evoked p...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-008-1319-7

    authors: Müller-Dahlhaus JF,Orekhov Y,Liu Y,Ziemann U

    更新日期:2008-05-01 00:00:00

  • Enactment effect in memory: evidence concerning the function of the supramarginal gyrus.

    abstract::Experimental behavioral data show that written action descriptions are remembered better when encoded by enacting them compared with merely verbal encoding. To explore this facilitating effect of encoding by performing actions ('enactment effect'), a functional magnetic resonance imaging (fMRI) study was conducted wit...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/s00221-003-1398-4

    authors: Russ MO,Mack W,Grama CR,Lanfermann H,Knopf M

    更新日期:2003-04-01 00:00:00

  • Human optokinetic nystagmus is linked to the stereoscopic system.

    abstract::It was previously proposed that a linkage between the optokinetic system and the stereoscopic system in higher mammals serves to allow these animals to selectively stabilize those parts of the visual scene which lie in the plane of convergence as the animals move forward in a three-dimensional world (Howard and Ohmi, ...

    journal_title:Experimental brain research

    pub_type: 杂志文章

    doi:10.1007/BF00228902

    authors: Howard IP,Simpson WA

    更新日期:1989-01-01 00:00:00