Abstract：
:Mutations induced by radiation are widely used for developing new varieties of plants. To better understand the frequency and pattern of irradiation-induced chromosomal rearrangements, we irradiated the dry seeds of Chinese Spring (CS)-Dasypyrum villosum nullisomic-tetrasomic (6A/6D) addition (6V) line (2n = 44), WD14, with (60)Co-γ-rays at dosages of 100, 200, and 300 Gy. The M₀ and M₁ generations were analyzed using Feulgen staining and non-denaturing fluorescence in situ hybridization (ND-FISH) by using oligonucleotide probes. Abnormal mitotic behavior and chromosomes with structural changes were observed in the M₀ plants. In all, 39 M₁ plants had structurally changed chromosomes, with the B genome showing the highest frequency of aberrations and tendency to recombine with chromosomes of the D genome. In addition, 19 M₁ plants showed a variation in chromosome number. The frequency of chromosome loss was considerably higher for 6D than for the alien chromosome 6V, indicating that 6D is less stable after irradiation. Our findings suggested that the newly obtained γ-induced genetic materials might be beneficial for future wheat breeding programs and functional gene analyses.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Zhang J,Jiang Y,Guo Y,Li G,Yang Z,Xu D,Xuan Pdoi
10.3390/ijms161226134subject
Has Abstractpub_date
2015-12-14 00:00:00pages
29787-96issue
12issn
1422-0067pii
ijms161226134journal_volume
16pub_type
杂志文章abstract：:Drought is one of the major stress factors affecting the growth and development of plants. In this context, drought-related losses of crop plant productivity impede sustainable agriculture all over the world. In general, plants respond to water deficits by multiple physiological and metabolic adaptations at the molecu...
journal_title：International journal of molecular sciences
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abstract：BACKGROUND:Ras homolog gene family member A (RhoA) is involved in Wnt-5a-induced migration of gastric and breast cancer cells. We investigated the roles of RhoA and Wnt-5a in ovarian carcinoma. METHODS:RhoA and Wnt-5a mRNA and protein expression in normal fallopian tube epithelium, benign tumors, primary ovarian carci...
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journal_title：International journal of molecular sciences
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更新日期：2017-12-21 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2018-04-09 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2019-10-23 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2018-11-24 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2018-05-02 00:00:00
