当前位置： SCI文献检索 > INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE期刊下所有文献 > Measuring In Vivo Free Radical Production by the Outer Retina.

Measuring In Vivo Free Radical Production by the Outer Retina.

Abstract：

PURPOSE:Excessive and continuously produced free radicals in the outer retina are implicated in retinal aging and the pathogenesis of sight-threatening retinopathies, yet measuring outer retinal oxidative stress in vivo remains a challenge. Here, we test the hypothesis that continuously produced paramagnetic free radicals from the outer retina can be measured in vivo using high-resolution (22-μm axial resolution) 1/T1magnetic resonance imaging (MRI) without and with a confirmatory quench (quench-assisted MRI). METHODS:Low-dose sodium iodate-treated and diabetic C57Bl6/J mice (and their controls), and rod-dominated (129S6) or cone-only R91W;Nrl-/- mice were studied. In dark-adapted groups, 1/T1 was mapped transretinally in vivo without or with (1) the antioxidant combination of methylene blue (MB) and α-lipoic acid (LPA), or (2) light exposure; in subgroups, retinal superoxide production was measured ex vivo (lucigenin). RESULTS:In the sodium iodate model, retinal superoxide production and outer retina-specific 1/T1 values were both significantly greater than normal and corrected to baseline with MB+LPA therapy. Nondiabetic mice at two ages and 1.2-month diabetic mice (before the appearance of oxidative stress) had similar transretinal 1/T1 profiles. By 2.3 months of diabetes, only outer retinal 1/T1 values were significantly greater than normal and were corrected to baseline with MB+LPA therapy. In mice with healthy photoreceptors, a light quench caused 1/T1 of rods, but not cones, to significantly decrease from their values in the dark. CONCLUSIONS:Quench-assisted MRI is a feasible method for noninvasively measuring normal and pathologic production of free radicals in photoreceptors/RPE in vivo.

journal_name

Invest Ophthalmol Vis Sci

journal_title

Investigative ophthalmology & visual science

authors

Berkowitz BA,Bredell BX,Davis C,Samardzija M,Grimm C,Roberts R

doi

10.1167/iovs.15-18420

subject

Has Abstract

pub_date

2015-12-01 00:00:00

pages

7931-8

issue

13

eissn

0146-0404

issn

1552-5783

pii

2476650

journal_volume

56

pub_type

杂志文章

相关文献

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE文献大全
  • Analysis of normal flicker sensitivity and its variability in the visuogram test.

    abstract：:Flicker sensitivity was measured in groups of younger and older adult observers to assess its mean values and the test-retest variability both between sessions and in a single session. Test-retest sensitivities differed by less than 15% but tended to decrease slightly in a session and increase slightly between session...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Tyler CW

    更新日期：1991-08-01 00:00:00

  • Pericyte chemomechanics and the angiogenic switch: insights into the pathogenesis of proliferative diabetic retinopathy?

    abstract：PURPOSE:To establish the regulatory roles that pericytes have in coordinating retinal endothelial cell (EC) growth and angiogenic potential. METHODS:Pericytes were derived from donor diabetic (DHuRP) or normal (NHuRP) human retinae, and characterized using vascular markers, coculture, contraction, morphogenesis, and p...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.14-13945

    authors： Durham JT,Dulmovits BM,Cronk SM,Sheets AR,Herman IM

    更新日期：2015-06-01 00:00:00

  • Selective interactions of lymphocytes with neonatal and adult lacrimal gland tissues.

    abstract：:The lacrimal gland is a functional part of the mucosal immune system and is populated by lymphoid cells that begin to appear early in neonatal development. To define the events controlling the accumulation of these cells, an in vitro adherence assay was used to investigate the interactions of lymphocyte populations wi...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： O'Sullivan NL,Montgomery PC

    更新日期：1990-08-01 00:00:00

  • Fundus Autofluorescence Lifetime Patterns in Retinitis Pigmentosa.

    abstract：Purpose:We investigated whether fundus autofluorescence (FAF) lifetimes in patients with retinitis pigmentosa display a disease-specific lifetime pattern. Methods:Fundus autofluorescence lifetime imaging ophthalmoscopy (FLIO) was performed in two spectral channels (498-560 and 560-720 nm) after excitation with a 473 n...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.17-23336

    authors： Dysli C,Schuerch K,Escher P,Wolf S,Zinkernagel MS

    更新日期：2018-04-01 00:00:00

  • Migration of retinal cells through a perforated membrane: implications for a high-resolution prosthesis.

    abstract：PURPOSE:One of the critical difficulties in design of a high-resolution retinal implant is the proximity of stimulating electrodes to the target cells. This is a report of a phenomenon of retinal cellular migration into a perforated membrane that may help to address this problem. METHODS:Mylar membranes with an array ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.03-1327

    authors： Palanker D,Huie P,Vankov A,Aramant R,Seiler M,Fishman H,Marmor M,Blumenkranz M

    更新日期：2004-09-01 00:00:00

  • The Relation Between Light-Induced Lacrimation and the Melanopsin-Driven Postillumination Pupil Response.

    abstract：Purpose:To investigate the chromatic characteristics and intensity-response function of light-induced reflex lacrimation and its correlation with the melanopsin-driven postillumination pupil response (PIPR). Methods:Eleven visually normal participants completed the experiment. Lacrimation was measured in one eye by pl...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.16-21285

    authors： Lei S,Goltz HC,Chen X,Zivcevska M,Wong AM

    更新日期：2017-03-01 00:00:00

  • In Vivo Three-Dimensional Lamina Cribrosa Strains in Healthy, Ocular Hypertensive, and Glaucoma Eyes Following Acute Intraocular Pressure Elevation.

    abstract：Purpose:To compare in vivo lamina cribrosa (LC) strains (deformations) following acute IOP elevation in healthy, glaucoma, and ocular hypertensive subjects. Methods:There were 20 healthy, 20 high-tension primary open-angle glaucoma (POAG), 16 primary angle-closure glaucoma (PACG), and 20 ocular hypertensive (OHT; with...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.17-21982

    authors： Beotra MR,Wang X,Tun TA,Zhang L,Baskaran M,Aung T,Strouthidis NG,Girard MJA

    更新日期：2018-01-01 00:00:00

  • Fibroblast growth factor receptor-1, interleukin-1 receptor, and glucocorticoid receptor messenger RNA production in the human lacrimal gland.

    abstract：PURPOSE:To determine whether messenger RNA coding for fibroblast growth factor receptor-1, interleukin-1 receptor, and glucocorticoid receptor messenger RNA are produced in human lacrimal tissue. METHODS:Total cellular RNA was isolated from three specimens of normal human lacrimal tissue and complementary DNA was synt...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Wilson SE,Lloyd SA,Kennedy RH

    更新日期：1993-05-01 00:00:00

  • Regional differences in functional receptor distribution and calcium mobilization in the intact human lens.

    abstract：PURPOSE:To investigate regional differences in Ca(2+) mobilization kinetics in the intact human lens produced by exposure to agonists of tyrosine-kinase and G-protein-coupled receptors and to characterize the major receptor subtypes involved in Ca(2+) signaling in the different regions. METHODS:Whole human lenses were...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Collison DJ,Duncan G

    更新日期：2001-09-01 00:00:00

  • Preservation of visual sensitivity of older subjects: association with macular pigment density.

    abstract：PURPOSE:The authors investigated how individual differences in macular pigment (MP) density are related to loss of visual sensitivity with age. METHODS:Macular pigment and visual sensitivity of 27 healthy older subjects (aged 60-84 years) were compared with data from 10 younger subjects (aged 24-36 years). Macular pig...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Hammond BR Jr,Wooten BR,Snodderly DM

    更新日期：1998-02-01 00:00:00

  • Regulation of matrix metalloproteinase expression by tumor necrosis factor in a murine model of retinal neovascularization.

    abstract：PURPOSE:Hypoxia and growth factors are postulated to be involved in the development of retinal neovascularization through the regulation of extracellular proteinase production. It has been shown that matrix metalloproteinases (MMPs) are elevated in the retina during the neovascularization process. However, the factors ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Majka S,McGuire PG,Das A

    更新日期：2002-01-01 00:00:00

  • Internal reflectivity of filtering blebs versus intraocular pressure in patients with recent trabeculectomy.

    abstract：PURPOSE:The authors conducted a morphology analysis of filtering blebs with anterior segment optical coherence tomography (AS-OCT) in patients shortly after trabeculectomy to find further criteria for success or failure of a filtering bleb. METHODS:Thirty-seven patients (43 eyes) who had recently (<2 years) undergone ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.10-5520

    authors： Pfenninger L,Schneider F,Funk J

    更新日期：2011-04-14 00:00:00

  • Pressure-dependent changes in nuclei and the process origins of the endothelial cells lining Schlemm's canal.

    abstract：:Monkeys eyes were fixed with glutaraldehyde in vivo at positive intraocular pressure of 35 or 25 mm Hg and compared with eyes fixed without a positive pressure gradient, with the use of light microscopy and transmission and scanning electron microscopy. The entire endothelial lining of the inner wall of Schlemm's cana...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Johnstone MA

    更新日期：1979-01-01 00:00:00

  • A Normative Data Set for the Clinical Assessment of Achromatic and Chromatic Contrast Sensitivity Using a qCSF Approach.

    abstract：Purpose:The measurement of achromatic sensitivity has been an important tool for monitoring subtle changes in vision as the result of disease or response to therapy. In this study, we aimed to provide a normative data set for achromatic and chromatic contrast sensitivity functions within a common cone contrast space us...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.17-21645

    authors： Kim YJ,Reynaud A,Hess RF,Mullen KT

    更新日期：2017-07-01 00:00:00

  • Type XVII collagen (BP 180) in the developing avian cornea.

    abstract：PURPOSE:Previous sequence analyses of hemidesmosomal BP 180/collagen XVII cDNA from human skin and of a similar chicken corneal cDNA showed some similarities, but major differences as well. The authors examined whether, in one species, the same mRNA is present in cornea and skin. They also studied the developmental exp...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Gordon MK,Fitch JM,Foley JW,Gerecke DR,Linsenmayer C,Birk DE,Linsenmayer TF

    更新日期：1997-01-01 00:00:00

  • ITE, a novel endogenous nontoxic aryl hydrocarbon receptor ligand, efficiently suppresses EAU and T-cell-mediated immunity.

    abstract：PURPOSE:Ligands for aryl hydrocarbon receptor (AHR), such as dioxins, are highly toxic. One such ligand, TCDD, was found to exert potent immunosuppressive capacities in mice developing pathogenic autoimmune processes, including EAU, but its toxicity makes it unusable for humans. A recently identified endogenous AHR lig...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.12-11479

    authors： Nugent LF,Shi G,Vistica BP,Ogbeifun O,Hinshaw SJ,Gery I

    更新日期：2013-11-13 00:00:00

  • Decreased anionic sites in Bruch's membrane of spontaneous and drug-induced diabetes.

    abstract：:The basal laminae of capillaries, glomeruli, and nerves are thickened in diabetes. Previous studies have shown that diabetic tissues produce increased levels of basal lamina collagen and reduced levels of proteoglycans. Since heparan sulfate and chondroitin sulfate proteoglycans are thought to act as anionic barriers ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Caldwell RB,Slapnick SM,McLaughlin BJ

    更新日期：1986-12-01 00:00:00

  • Enthacrynic and acid effects on inner wall pores in living monkeys.

    abstract：PURPOSE:The influence of the inner wall of Schlemm's canal on aqueous outflow facility remains poorly understood. We examined the relationship between inner wall pore characteristics and outflow facility in living primate eyes in which facility had been pharmacologically increased by ethacrynic acid (ECA) infusion and ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Ethier CR,Croft MA,Coloma FM,Gangnon RE,Ladd W,Kaufman PL

    更新日期：1999-06-01 00:00:00

  • Fixational Saccades and Their Relation to Fixation Instability in Strabismic Monkeys.

    abstract：Purpose:To evaluate the contribution of fixational saccades toward fixation instability in strabismic monkeys. Methods:Binocular eye movements were measured as six experimental monkeys (five strabismic monkeys and one monkey with downbeat nystagmus) and one normal monkey fixated targets of two shapes (Optotype, Disk) ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.17-22389

    authors： Upadhyaya S,Pullela M,Ramachandran S,Adade S,Joshi AC,Das VE

    更新日期：2017-11-01 00:00:00

  • The prevalence and incidence of epiretinal membranes in eyes with inactive extramacular CMV retinitis.

    abstract：PURPOSE:To determine the prevalence and incidence of epiretinal membranes (ERM) in eyes with inactive extramacular cytomegalovirus (CMV) retinitis in patients with acquired immune deficiency syndrome (AIDS). METHODS:A case-control report from a longitudinal multicenter observational study by the Studies of the Ocular ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,多中心研究

    doi：10.1167/iovs.14-14479

    authors： Kozak I,Vaidya V,Van Natta ML,Pak JW,May KP,Thorne JE,Studies of the Ocular Complications of AIDS Research Group.

    更新日期：2014-06-12 00:00:00

  • Evidence for a major gene for cortical cataract.

    abstract：PURPOSE:To examine the possible presence of a major gene determining susceptibility to cortical cataract. METHODS:The percentage of the lens area involved with cortical opacity, summed over both eyes, was evaluated in 1275 individuals from the Beaver Dam Eye Study. After adjusting for the effects of age and sex, these...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Heiba IM,Elston RC,Klein BE,Klein R

    更新日期：1995-01-01 00:00:00

  • Synthesis of type II interleukin-1 receptors by human corneal epithelial cells but not by keratocytes.

    abstract：PURPOSE:The purpose of this study was to determine whether human corneal epithelial cells and keratocytes synthesize both the soluble and membrane forms of the type II IL-1 receptor (IL-1RII). METHODS:Primary cell cultures of human corneal epithelial cells and keratocytes were established from human corneas. RT-PCR wa...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Cubitt CL,Lausch RN,Oakes JE

    更新日期：2001-03-01 00:00:00

  • Transplantation of tissue-engineered epithelial cell sheets after excimer laser photoablation reduces postoperative corneal haze.

    abstract：PURPOSE:To apply tissue-engineered cell sheet transplantation after excimer laser keratectomy as a novel approach for the reduction of postoperative corneal haze. METHODS:Limbal biopsy specimens were obtained, and epithelial cells were cultured on temperature-responsive culture inserts without the use of feeder cells....

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.05-0995

    authors： Hayashida Y,Nishida K,Yamato M,Yang J,Sugiyama H,Watanabe K,Hori Y,Maeda N,Kikuchi A,Okano T,Tano Y

    更新日期：2006-02-01 00:00:00

  • Disruption of the basement membrane after corneal débridement.

    abstract：PURPOSE:To determine whether the native basement membrane left behind after manual débridement wounding is retained throughout healing in the Balb/c mouse. METHODS:Mouse corneas were subjected to either 1.5 mm (small) or limbus-to-limbus (large) epithelial débridement wounds and allowed to heal for times ranging from ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Sta Iglesia DD,Stepp MA

    更新日期：2000-04-01 00:00:00

  • Effect of diabetic macular edema on peripapillary retinal nerve fiber layer thickness profiles.

    abstract：PURPOSE:To investigate both the effect of diabetic macular edema (DME) on measured peripapillary retinal nerve fiber layer (RNFL) thickness and the effect of intravitreal bevacizumab injection on RNFL thickness using spectral-domain optical coherence tomography (SD-OCT) in patients with diabetic retinopathy. METHODS:W...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.13-13776

    authors： Hwang DJ,Lee EJ,Lee SY,Park KH,Woo SJ

    更新日期：2014-05-15 00:00:00

  • Expression of matrix metalloproteinases and inhibitor by human trabecular meshwork.

    abstract：:Extracellular matrix (ECM) turnover and remodeling are initiated, at least in part, by the regulated secretion of members of a family of matrix metalloproteinases. Human and bovine trabecular mesh-work in culture secrete interstitial collagenase, both the 72- and the 92-kD forms of type IV collagenase (gelatinases) an...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Alexander JP,Samples JR,Van Buskirk EM,Acott TS

    更新日期：1991-01-01 00:00:00

  • Automated assessment of diabetic retinal image quality based on clarity and field definition.

    abstract：PURPOSE:To evaluate the performance of an automated retinal image quality assessment system for use in automated diabetic retinopathy grading. METHODS:Algorithmic methods have been developed for assessing the quality of 45 degrees single field retinal images for use in diabetic retinopathy screening. For this purpose,...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.05-1155

    authors： Fleming AD,Philip S,Goatman KA,Olson JA,Sharp PF

    更新日期：2006-03-01 00:00:00

  • Connexin43 knockdown accelerates wound healing but inhibits mesenchymal transition after corneal endothelial injury in vivo.

    abstract：PURPOSE:To explore connexin43 (Cx43) knockdown as an efficient treatment for corneal endothelial injury in an in vivo rat corneal scrape injury model. METHODS:Scrape injury was induced in the corneal endothelium, and immunolabeling (ZO-1, alpha-SMA, Cx43) was performed to analyze changes in Cx43 expression during woun...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.07-0255

    authors： Nakano Y,Oyamada M,Dai P,Nakagami T,Kinoshita S,Takamatsu T

    更新日期：2008-01-01 00:00:00

  • Size threshold perimetry performs as well as conventional automated perimetry with stimulus sizes III, V, and VI for glaucomatous loss.

    abstract：PURPOSE:It is thought that large perimetric stimuli are insensitive for demonstrating visual field defects. To test the hypothesis that there is no difference in the total number of abnormal test locations with total deviation empiric probability plots in glaucoma patients, we compared results of glaucoma patients test...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.12-11300

    authors： Wall M,Doyle CK,Eden T,Zamba KD,Johnson CA

    更新日期：2013-06-07 00:00:00

  • Automated retinal topographic maps measured with magnetic resonance imaging.

    abstract：PURPOSE:Recent studies on ocular shape have raised increased interest in the peripheral characteristics of the eye, as it potentially triggers changes in the central vision. Current techniques are, however, not capable of accurately measuring the three-dimensional shape of the retina. We describe a new magnetic resonan...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.14-15161

    authors： Beenakker JW,Shamonin DP,Webb AG,Luyten GP,Stoel BC

    更新日期：2015-01-15 00:00:00