Abstract:
AIM:To investigate the effects of the cross-linking agent cinnamaldehyde (CA) on differentiation of human dental pulp cells (hDPCs) cultured in a collagen hydrogel, which may be useful as a scaffold for regenerative endodontic therapy. METHODOLOGY:The odontogenic potential of hDPCs exposed to CA was examined using alkaline phosphatase (ALP) activity, Alizarin red S staining and real-time polymerase chain reaction for odontogenic gene expression. The morphological features of hDPCs cultured in CA-treated collagen were evaluated by scanning electron microscopy. Determination of cell numbers for evaluating proliferation was assessed by optical and fluorescence microscopy. To assess the mechanical properties of collagen treated with CA, setting time, compressive strength and surface roughness were measured. Statistical analysis was performed using Student's t-test compared with control (P = 0.05). RESULTS:CA per se did not increase ALP activity, calcium nodule formation and expression of odontogenic-related markers (P > 0.05). On the contrary, the proliferation and odontogenic differentiation of hDPCs cultured in a collagen scaffold was promoted in the presence of CA (P < 0.05). The setting time was significantly shortened, and the compressive strength and surface roughness were increased by treatment with CA (P < 0.05). CONCLUSIONS:Cross-linking of collagen scaffolds by CA had beneficial effects with respect to attachment, proliferation and differentiation of hDPCs. Consequently, the application of cross-linking agents such as CA may represent a new strategy for dentine-pulp complex regeneration.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Kwon YS,Lee SH,Hwang YC,Rosa V,Lee KW,Min KSdoi
10.1111/iej.12592subject
Has Abstractpub_date
2017-01-01 00:00:00pages
58-66issue
1eissn
0143-2885issn
1365-2591journal_volume
50pub_type
杂志文章abstract:AIM:To investigate a potential cause-effect relationship between dentinal microcracks and fracture resistance of mandibular incisors that had not been endodontically treated. METHODOLOGY:Sixty mandibular incisors with circular-shaped canals were selected based on micro-computed tomographic scans to create a homogeneou...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13370
更新日期:2020-11-01 00:00:00
abstract:AIM:To investigate the expression of TLR2 in refractory periapical lesions. METHODOLOGY:Refractory periapical lesion biopsies were histopathologically and clinically categorized into asymptomatic periapical granuloma (n=10), symptomatic periapical granuloma (n=10) or periapical cyst (n=10) and prepared for immunohisto...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01893.x
更新日期:2011-10-01 00:00:00
abstract:AIM:The purpose was to present the frequency of periapical healing in first and second/third mandibular molars, after root-end resections sealed with a dentine-bonded resin composite. METHODOLOGY:Root-end sealing of resected mandibular molar roots was made with dentine-bonded composite (Gluma-Retroplast) as a cover on...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2001.00383.x
更新日期:2001-06-01 00:00:00
abstract::This position statement on the management of deep caries and the exposed pulp represents the consensus of an expert committee, convened by the European Society of Endodontology (ESE). Preserving the pulp in a healthy state with sustained vitality, preventing apical periodontitis and developing minimally invasive biolo...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13080
更新日期:2019-07-01 00:00:00
abstract:AIM:To devise an optimal protocol and to analyse the leucocyte composition of periapical (PA) lesions by flow cytometry. METHODOLOGY:PA lesions were mechanically agitated, with and without proteolysis. This was with either 0.2% collagenase alone, or in combination with 0.02% DNA-ase in serial incubations until all tis...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2000.00294.x
更新日期:2000-03-01 00:00:00
abstract:AIM:To evaluate ex vivo the accuracy of two electronic apex locators during root canal length determination in primary incisor and molar teeth with different stages of physiological root resorption. METHODOLOGY:One calibrated examiner determined the root canal length in 17 primary incisors and 16 primary molars (total...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01366.x
更新日期:2008-04-01 00:00:00
abstract::The objective of this study was to compare the adaptation of mechanically softened gutta-percha to the root canal wall in the presence and absence of smear layer. The root canals of 20 freshly extracted human maxillary incisors were cleaned and shaped. Prior to obturation, 10 root canals were irrigated with 20 ml of 5...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1995.tb00312.x
更新日期:1995-09-01 00:00:00
abstract:AIM:To characterize the microbial composition of persistent periapical lesions of root filled teeth using a molecular genetics approach. METHODOLOGY:Apical lesion samples were collected from 12 patients (23-80 years old) who visited the Kyushu University Hospital for apicectomy with persistent periapical lesions assoc...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12361
更新日期:2015-08-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12961
更新日期:2019-01-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01160.x
更新日期:2006-11-01 00:00:00
abstract::Dentoalveolar ankylosis is a common complication after replantation of teeth with injured periodontal ligaments. This is a serious complication for the tooth since it leads to progressive root resorption. In young individuals it may also cause infra-occlusion and reduced development of the alveolar process. The purpos...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1993.tb00549.x
更新日期:1993-03-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12547
更新日期:2016-10-01 00:00:00
abstract:AIM:The aim of this study was to determine the shaping ability of GT Rotary Files in simulated root canals. METHODOLOGY:Forty canals with four different shapes in terms of angle (40 degrees and 60 degrees) and position of curvature (straight section before curve: 8 and 12 mm) were prepared using a crown-down/stepback ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00540.x
更新日期:2002-07-01 00:00:00
abstract:AIM:To investigate the prevalence of apical periodontitis and the quality of root canal treatment in an adult Turkish population who visited a university dental clinic. SUMMARY:The periapical status of 8863 teeth belonging to 375 patients was evaluated by two pre-calibrated observers from panoramic radiographs. The pr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01217.x
更新日期:2007-02-01 00:00:00
abstract:AIM:To compare several parameters of root canal preparation using two different rotary nickel-titanium (Ni-Ti) instruments: FlexMaster (VDW, Munich, Germany) and HERO 642 (Micro-Mega, Besançon, France). METHODOLOGY:Fifty extracted human mandibular molars with root canal curvatures between 20 and 40 degrees were embedd...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2003.00664.x
更新日期:2003-05-01 00:00:00
abstract:AIM:To evaluate the effect of multiple refreshment/activation cycles and temperature on the reaction rate of sodium hypochlorite (NaOCl) with bovine dentine during ultrasonic activated irrigation (UAI) under laboratory conditions. METHODOLOGY:The root canal walls of 24 standardized root canals in bovine incisors were ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12125
更新日期:2014-02-01 00:00:00
abstract::The use of a resin-based glass ionomer as an endodontic sealer was studied in vitro. The root canals of extracted single-rooted human teeth with mature apices were prepared using a stepback technique. Subsequently, the smear layer was removed with 40% citric acid and the canals obturated using lateral condensation of ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1992.tb01156.x
更新日期:1992-09-01 00:00:00
abstract:AIM:To provide insight into the principles of operation of electronic apex locators, determine optimal measuring parameters of the impedance ratio method, and to evaluate its accuracy. METHODOLOGY:Electrical impedance was measured ex vivo on 14 extracted human teeth using a QuadTech 1920 precision impedance analyzer. ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01589.x
更新日期:2009-09-01 00:00:00
abstract:AIM:To determine the influence of filling technique and root canal area on the percentage of gutta-percha (PGP) in laterally compacted root fillings. METHODOLOGY:Sixty extracted canine teeth were accessed and the root canals instrumented to the same size. They were then divided in three groups and filled with laterall...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01575.x
更新日期:2009-08-01 00:00:00
abstract:AIM:To clarify the mechanisms of inflammatory cell migration in human periapical granulomas by examining vascular endothelial (VE) cadherin and inducible nitric oxide synthase (iNOS)-producing cells. METHODOLOGY:Periapical tissues were obtained from patients during endodontic surgery and were divided into two portions...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01068.x
更新日期:2006-03-01 00:00:00
abstract::The cutting heads of the first 18 instruments in six sets of clinically used Lightspeed instruments (sizes 20-65) were examined in a scanning electron microscope (SEM). Three sets had been used by full-time faculty and discarded as follows: instrument sizes 20-32.5 after 18 treated root canals; instrument sizes 35-47....
journal_title:International endodontic journal
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract:AIM:To compare dentinal microcrack formation whilst using Ni-Ti hand K-files, ProTaper hand and rotary files and the WaveOne reciprocating file. METHODOLOGY:One hundred and fifty mandibular first molars were selected. Thirty teeth were left unprepared and served as controls, and the remaining 120 teeth were divided in...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12197
更新日期:2014-07-01 00:00:00
abstract:AIM:To report the hydration mechanism of white mineral trioxide aggregate (White MTA, Dentsply, Tulsa Dental Products, Tulsa, OK, USA). METHODOLOGY:The chemical constitution of white MTA was studied by viewing the powder in polished sections under the scanning electron microscope (SEM). The hydration of both white MTA...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01248.x
更新日期:2007-06-01 00:00:00
abstract::Root-canal-filling materials are either placed directly onto vital periapical tissues or may leach through dentine. The tissue response to these materials therefore becomes important and may influence the outcome of endodontic treatment. This paper is a review of the biocompatibility of contemporary orthograde and ret...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.2003.00637.x
更新日期:2003-03-01 00:00:00
abstract:AIMS:To evaluate the microbiota of endodontic infections in deciduous teeth by Checkerboard DNA-DNA hybridization after uniform amplification of DNA in samples by multiple displacement amplification (MDA). METHODOLOGY:Forty samples from the root canal system of deciduous teeth exhibiting pulp necrosis with or without ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01805.x
更新日期:2011-03-01 00:00:00
abstract:AIM:To identify the role of astrocyte elevated gene-1 (AEG-1) and the mechanism underlying the inflammatory response in dental pulp cells. METHODOLOGY:Astrocyte elevated gene-1 expression was detected at different stages of pulpitis in a rat model using immunohistochemistry. RT-qPCR and Western blot were used to asses...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12921
更新日期:2018-10-01 00:00:00
abstract:AIM:The objectives of this laboratory-based study were to investigate the effects of GH12 on Enterococcus faecalis biofilm and virulence. METHODOLOGY:Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of GH12 against E. faecalis were first determined. A time-kill assay was further cond...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13296
更新日期:2020-07-01 00:00:00
abstract:AIM:To determine the influence of various irrigating solutions on the accuracy of the electronic apex locator facility in the Tri Auto ZX handpiece. METHODOLOGY:One hundred and forty teeth with single canals and mature apices, scheduled for extraction for either periodontal or prosthetic reasons in 76 patients were us...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2007.01234.x
更新日期:2007-05-01 00:00:00
abstract:AIM:The aim of this study was to determine the shaping ability of Quantec LX nickel-titanium rotary instruments in simulated root canals. METHODOLOGY:Forty simulated canals consisting of four different shapes in terms of angle and position of curvature were prepared with Quantec LX instruments. Sequential still images...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2000.00311.x
更新日期:2000-07-01 00:00:00
abstract:AIM:To evaluate the effect of irrigation with 5% sodium hypochlorite (NaOCl) alone and in conjunction with 17% ethylenediaminetetraacetic acid (EDTA) on tooth surface strain (TSS) and to analyse the influence of irrigation time, root morphology and dentine thickness. METHODOLOGY:Thirty-six single-rooted pre-molars wit...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01655.x
更新日期:2010-03-01 00:00:00