Astrocyte elevated gene-1 participates in the production of pro-inflammatory cytokines in dental pulp cells via NF-κB signalling pathway.

Abstract:

AIM:To identify the role of astrocyte elevated gene-1 (AEG-1) and the mechanism underlying the inflammatory response in dental pulp cells. METHODOLOGY:Astrocyte elevated gene-1 expression was detected at different stages of pulpitis in a rat model using immunohistochemistry. RT-qPCR and Western blot were used to assess AEG-1 expression in human dental pulp cells stimulated by lipopolysaccharide (LPS). The LPS-induced expression of inflammatory cytokine genes was quantified by RT-qPCR in cells transfected with AEG-1 or negative control (NC) siRNA. Immunofluorescence and Western blot were utilized to evaluate the activation of NF-κB signalling in AEG-1 knockdown cells. AEG-1 expression was also investigated by Western blot in dental pulp cells pre-treated with inhibitors of NF-κB and PI-3K signalling before the addition of LPS. Data were analysed statistically using one-way anova. RESULTS:The exposure of dental pulp tissue to LPS resulted in acute inflammation with necrosis and AEG-1 expression in the pulp tissue beneath the perforation. In LPS-stimulated dental pulp cells, AEG-1 mRNA and protein were significantly up-regulated (P < 0 .05) in a time- and dose-dependent manner. In AEG-1 knockdown cells, the synthesis of IL-1, IL-6 and TNF-α mRNA was suppressed significantly (P < 0.05) upon LPS induction. AEG-1 knockdown also inhibited nuclear translocation of p65. Suppression of NF-κB and PI-3K abrogated the LPS-induced up-regulation of AEG-1. CONCLUSION:Astrocyte elevated gene-1 participated in inflammatory cytokine synthesis via NF-κB signalling in the dental pulp. Both NF-κB and PI-3K signalling are involved in LPS-induced AEG-1 expression in dental pulp cells.

journal_name

Int Endod J

authors

Wu H,He M,Yang R,Zuo Y,Bian Z

doi

10.1111/iej.12921

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

1130-1138

issue

10

eissn

0143-2885

issn

1365-2591

journal_volume

51

pub_type

杂志文章
  • Agreement between 2D and 3D radiographic outcome assessment one year after periapical surgery.

    abstract:AIM:To compare 2D with 3D radiography in assessing the treatment outcome 1 year after periapical surgery. METHODOLOGY:In this prospective study, periapical radiographs (PA) and cone beam computed tomography (CBCT) were performed 1 year after periapical surgery. Three calibrated observers independently evaluated the ra...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12548

    authors: von Arx T,Janner SF,Hänni S,Bornstein MM

    更新日期:2016-10-01 00:00:00

  • Porosity and sealing ability of root fillings with gutta-percha and BioRoot RCS or AH Plus sealers. Evaluation by three ex vivo methods.

    abstract:AIM:To investigate the ability of BioRoot RCS, a tricalcium silicate-based root canal sealer and AH Plus to effectively fill the root canals of contralateral teeth using three evaluation methods, and to investigate also the correlation between the methods. METHODOLOGY:The prepared root canals of ten pairs of contralat...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12513

    authors: Viapiana R,Moinzadeh AT,Camilleri L,Wesselink PR,Tanomaru Filho M,Camilleri J

    更新日期:2016-08-01 00:00:00

  • Comparison of two laser Doppler systems on the measurement of blood flow of premolar teeth under different pulpal conditions.

    abstract::An in vivo investigation was conducted to assess the ability of two laser Doppler flowmeters (DRT4, Moor Instruments Ltd; Laserflo BPM2, Vasamedics) to differentiate between vital pulp, ischaemically necrosed pulp, and an empty pulp chamber in premolar teeth in children. Sixteen intact, vital teeth in need of extracti...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.1997.00068.x

    authors: Mesaros S,Trope M,Maixner W,Burkes EJ

    更新日期:1997-05-01 00:00:00

  • Ex vivo comparison of two electronic apex locators with different scales and frequencies.

    abstract:AIM:To compare ex vivo the accuracy of two impedance quotient apex locators with different scales and frequencies of the measuring circuit. METHODOLOGY:In each root of 193 extracted human teeth, electronic working length determination (ELD) was carried out with a newly constructed measuring unit. In all cases, ELD was...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2005.01004.x

    authors: Hör D,Krusy S,Attin T

    更新日期:2005-12-01 00:00:00

  • Gingival and bone necrosis caused by accidental sodium hypochlorite injection instead of anaesthetic solution.

    abstract:AIM:To report clinical complications (pain, necrotic gingival tissue and bone sequestration) resulting from accidental injection of sodium hypochlorite. SUMMARY:Root canal treatment is a routine clinical procedure with few reported complications. Sodium hypochlorite (NaOCl) is commonly used as an irrigant during the p...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2007.01340.x

    authors: Pontes F,Pontes H,Adachi P,Rodini C,Almeida D,Pinto D Jr

    更新日期:2008-03-01 00:00:00

  • Root canal anatomy of mandibular second molars. Part II. C-shaped canals.

    abstract::The root canal anatomy of 19 mandibular second molars with C-shaped canals was investigated by rendering the roots transparent and allowing the canal system to be observed by black ink infiltration. The presence of three root canals was most frequent, and lateral canals were found in all roots. Transverse anastomoses ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.1990.tb00801.x

    authors: Manning SA

    更新日期:1990-01-01 00:00:00

  • Morphometric study of the apical anatomy of C-shaped root canal systems in mandibular second molars.

    abstract:AIM:To investigate the apical anatomy of C-shaped canal systems in mandibular second molars by micro-computerized tomography (microCT) and stereomicroscopy. METHODOLOGY:Forty-four permanent mandibular second molars with a C-shaped root canal systems from a native Chinese population were scanned at 100 microm intervals...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2007.01193.x

    authors: Cheung GS,Yang J,Fan B

    更新日期:2007-04-01 00:00:00

  • Anatomical danger zone reconsidered: a micro-CT study on dentine thickness in mandibular molars.

    abstract:AIM:To investigate the smallest dentine thickness in mesial canals of mandibular molars along the cervical and middle thirds of the root by means of a micro-computed tomographic (micro-CT) technology and digital image analysis. METHODOLOGY:Fifty mesial roots of mandibular molars having two independent canals (mesiobuc...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.13141

    authors: De-Deus G,Rodrigues EA,Belladonna FG,Simões-Carvalho M,Cavalcante DM,Oliveira DS,Souza EM,Giorgi KA,Versiani MA,Lopes RT,Silva EJNL,Paciornik S

    更新日期:2019-10-01 00:00:00

  • Influence of sterilization on mechanical properties and fatigue resistance of nickel-titanium rotary endodontic instruments.

    abstract:AIM:To evaluate the effect of repeated sterilization cycles in dry oven or autoclave, on the mechanical behaviour and fatigue resistance of rotary endodontic Ni-Ti instruments. METHODOLOGY:New Ni-Ti instruments were subjected to five consecutive sterilization cycles in a dry oven or steam autoclave. Microhardness was ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2006.01138.x

    authors: Viana AC,Gonzalez BM,Buono VT,Bahia MG

    更新日期:2006-09-01 00:00:00

  • An in-vitro investigation of the antibacterial effect of nisin in root canals and canal wall radicular dentine.

    abstract:AIM:To determine whether nisin, a bacteriocin, would be effective at killing Enterococcus faecalis and Streptococcus gordonii cells in solution and within the root canal system. METHODOLOGY:Bacterial isolates of E. faecalis and S. gordonii were grown from glycerol stocks in closed tubes containing BHY broth at 37 degr...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2004.00846.x

    authors: Turner SR,Love RM,Lyons KM

    更新日期:2004-10-01 00:00:00

  • A maxillary lateral incisor with four root canals.

    abstract:AIM:To describe the successful non-surgical endodontic management of a permanent maxillary lateral incisor tooth with four root canals. SUMMARY:Aberrant morphology of root canals can occur in any tooth, including maxillary lateral incisors. In the presented case, clinical examination and radiographs clearly depicted t...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2011.01984.x

    authors: Kottoor J,Murugesan R,Albuquerque DV

    更新日期:2012-04-01 00:00:00

  • Shaping ability of BioRace, ProTaper NEXT and Genius nickel-titanium instruments in curved canals of mandibular molars: a MicroCT study.

    abstract:AIM:To evaluate and compare the canal shaping ability of BioRace, ProTaper NEXT and Genius engine-driven nickel-titanium (NiTi) file systems in extracted mandibular first molars using micro-computed tomography (MCT). METHODOLOGY:Sixty mesial root canals of mandibular first molars were randomly divided into three equal...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12961

    authors: Drukteinis S,Peciuliene V,Dummer PMH,Hupp J

    更新日期:2019-01-01 00:00:00

  • Antibiofilm activity of three irrigation protocols activated by ultrasonic, diode laser or Er:YAG laser in vitro.

    abstract:AIM:To investigate the impact of three irrigation protocols, activated by three different methods, on mature biofilms of Enterococcus faecalis in vitro. METHODOLOGY:Root canals in 280 single-rooted teeth were instrumented using a rotary Ni-Ti system. Biofilms of E. faecalis were generated based on a previously establi...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12354

    authors: Neelakantan P,Cheng CQ,Mohanraj R,Sriraman P,Subbarao C,Sharma S

    更新日期:2015-06-01 00:00:00

  • Microbiota of deciduous endodontic infections analysed by MDA and Checkerboard DNA-DNA hybridization.

    abstract:AIMS:To evaluate the microbiota of endodontic infections in deciduous teeth by Checkerboard DNA-DNA hybridization after uniform amplification of DNA in samples by multiple displacement amplification (MDA). METHODOLOGY:Forty samples from the root canal system of deciduous teeth exhibiting pulp necrosis with or without ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2010.01805.x

    authors: Tavares WL,Neves de Brito LC,Teles RP,Massara ML,Ribeiro Sobrinho AP,Haffajee AD,Socransky SS,Teles FR

    更新日期:2011-03-01 00:00:00

  • Periapical changes following root-canal treatment observed 20-27 years postoperatively.

    abstract:AIM:The aim of the present study was to identify periapical changes 20-27 years after root-canal treatment. METHODOLOGY:The periapical condition of 265 roots filled by undergraduate students was evaluated in two series of intraoral radiographs taken 10-17 and 20-27 years after treatment. Roots (72) not recorded with a...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.2002.00568.x

    authors: Molven O,Halse A,Fristad I,MacDonald-Jankowski D

    更新日期:2002-09-01 00:00:00

  • Fracture strength of bovine incisors after intra-radicular treatment with MTA in an experimental immature tooth model.

    abstract:AIM:To evaluate, using an experimental immature tooth model, the fracture resistance of bovine incisors submitted to different reinforcement treatments with mineral trioxide aggregate (MTA). METHODOLOGY:An immature tooth model was created by sectioning the coronal and apical portions of 40 bovine incisors 8 mm above a...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2007.01266.x

    authors: Bortoluzzi EA,Souza EM,Reis JM,Esberard RM,Tanomaru-Filho M

    更新日期:2007-09-01 00:00:00

  • A simple mounting model for consistent determination of the accuracy and repeatability of apex locators.

    abstract:AIM:To develop a precise and simple mounting model (MM) for evaluating apex locators and to compare the repeatability of the MM with a conventional visual method (VM). METHODOLOGY:Electronic working length determination was performed in 32 maxillary central incisors using two methods: (i) the MM method and (ii) a conv...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2006.01052.x

    authors: ElAyouti A,Löst C

    更新日期:2006-02-01 00:00:00

  • Dynamic torque and apical forces of ProFile.04 rotary instruments during preparation of curved canals.

    abstract:AIM:To analyse torque and force generated whilst shaping curved canals using rotary instruments. METHODOLOGY:A specially designed computer-controlled testing platform was used to record events during the shaping of straight and curved canals in plastic blocks and in extracted human teeth using ProFile.04 instruments. ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.0143-2885.2001.00494.x

    authors: Peters OA,Barbakow F

    更新日期:2002-04-01 00:00:00

  • Bovine teeth can reliably substitute human dentine in an intra-tooth push-out bond strength model?

    abstract:AIM:To investigate the reliability of using bovine teeth as an alternative to human teeth in an intra-tooth push-out model by comparing the bond strength of three root canal sealers in both tooth substrates. METHODOLOGY:From human and bovine incisors (12 per group), one 1-mm disc was obtained from each root. On each d...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.13085

    authors: Silva EJNL,Carvalho NK,Prado MC,Senna PM,Souza EM,De-Deus G

    更新日期:2019-07-01 00:00:00

  • Curved molar root canal preparations using Hero 642 rotary nickel-titanium instruments.

    abstract:AIM:The Bramante method was used to study how the natural curvature of root canals was modified when using the Hero 642 nickel-titanium rotary system. METHODOLOGY:Twenty-four canals were selected in freshly extracted human mandibular molars. Every root canal had an angle of curvature greater than 20 degrees. Roots wer...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.2001.00440.x

    authors: Bertrand MF,Lupi-Pégurier L,Médioni E,Muller M,Bolla M

    更新日期:2001-12-01 00:00:00

  • Vascular endothelial growth factor A/Vascular endothelial growth factor receptor 2 axis promotes human dental pulp stem cell migration via the FAK/PI3K/Akt and p38 MAPK signalling pathways.

    abstract:AIM:To investigate the effects of vascular endothelial growth factor A (VEGFA) and the underlying molecular mechanisms on the migration of human dental pulp stem cells (hDPSCs). METHODOLOGY:The expression of VEGFA in inflammatory pulp tissue and lipopolysaccharide (LPS)-stimulated dental pulp cells was examined by imm...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.13179

    authors: Sun X,Meng L,Qiao W,Yang R,Gao Q,Peng Y,Bian Z

    更新日期:2019-12-01 00:00:00

  • Cyclic fatigue resistance of ProTaper Next nickel-titanium rotary files.

    abstract:AIM:To compare the cyclic fatigue resistance of ProTaper Next files (PTN; Dentsply Maillefer, Ballaigues, Switzerland) with Twisted Files (TF; SybronEndo, Orange, CA, USA), HyFlex CM (HF; ColténeEndo/Whaledent, Inc, Cuyahoga Falls, OH, USA) and ProTaper Universal (PT; Dentsply Maillefer). METHODOLOGY:Size 25, .06 tape...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12244

    authors: Elnaghy AM

    更新日期:2014-11-01 00:00:00

  • Shear bond strength of Resilon to a methacrylate-based root canal sealer.

    abstract:AIM:To evaluate the adhesive strength of Resilon to Next root canal sealant (Heraeus-Kulzer), a methacrylate-based root canal sealer, using a modified microshear bond testing design. METHODOLOGY:Flat Resilon surfaces of different roughnesses (smooth surface and surface roughness equivalent to 320-grit and 180-grit) we...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2005.01012.x

    authors: Hiraishi N,Papacchini F,Loushine RJ,Weller RN,Ferrari M,Pashley DH,Tay FR

    更新日期:2005-10-01 00:00:00

  • Fate of vital pulps beneath a metal-ceramic crown or a bridge retainer.

    abstract:AIM:To investigate the incidence of and factors associated with pulpal necrosis in vital teeth restored with metal-ceramic crowns (CMCs) or crowned as part of a fixed-fixed bridge. METHODOLOGY:Patients who had a CMC or bridge retainer (BR) placed on a tooth with no previous history of root canal treatment from 1981 to...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2005.00982.x

    authors: Cheung GS,Lai SC,Ng RP

    更新日期:2005-08-01 00:00:00

  • Octenidine in root canal and dentine disinfection ex vivo.

    abstract:AIM:The aim of the present study was to investigate the antimicrobial activity of octenidine on Enterococcus faecalis ATCC 29212 in a dentine block model. METHODOLOGY:Fifty-six root segments of extracted human teeth were infected with E. faecalis for 4 weeks. Octenidine-phenoxyethanol gel (1 : 1) was applied for diffe...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2007.01279.x

    authors: Tandjung L,Waltimo T,Hauser I,Heide P,Decker EM,Weiger R

    更新日期:2007-11-01 00:00:00

  • Relationship between unintentional canal overfilling and the long-term outcome of primary root canal treatments and nonsurgical retreatments: a retrospective radiographic assessment.

    abstract:AIM:To determine retrospectively the long-term radiographic outcome of root canal treatments and root canal retreatments with unintentional root canal overfilling. METHODOLOGY:A total of 220 root canal treatments (143 primary/77 retreatments) with postoperative unintentional canal overfilling and performed by two endo...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.13209

    authors: Goldberg F,Cantarini C,Alfie D,Macchi RL,Arias A

    更新日期:2020-01-01 00:00:00

  • A new classification system for the restoration of root filled teeth.

    abstract::The aim of this report is to (i) review the current literature on the status of root filled teeth, (ii) analyse the most important factors in decision-making, (iii) discuss the current restorative concepts, and (iv) classify both the evidence and clinical practice in a way that seeks to be clear, understandable and he...

    journal_title:International endodontic journal

    pub_type: 杂志文章,评审

    doi:10.1111/iej.12847

    authors: Zarow M,Ramírez-Sebastià A,Paolone G,de Ribot Porta J,Mora J,Espona J,Durán-Sindreu F,Roig M

    更新日期:2018-03-01 00:00:00

  • European Society of Endodontology position statement: Surgical extrusion, intentional replantation and tooth autotransplantation: European Society of Endodontology developed by.

    abstract::This European Society of Endodontology (ESE) position statement on surgical extrusion, intentional replantation and tooth autotransplantation represents the consensus of an expert committee, convened by the ESE. A narrative review in the International Endodontic Journal (Plotino et al. 2020, international Endodontic J...

    journal_title:International endodontic journal

    pub_type: 杂志文章,评审

    doi:10.1111/iej.13456

    authors: Plotino G,Abella Sans F,Duggal MS,Grande NM,Krastl G,Nagendrababu V,Gambarini G

    更新日期:2021-01-26 00:00:00

  • The long-term sealing ability of an epoxy resin root canal sealer used with five gutta percha obturation techniques.

    abstract:AIM:The aim of the present study was to evaluate the long-term sealing ability (both apical and coronal) of an epoxy resin root canal sealer (AH26) when used with different gutta-percha obturation techniques. METHODOLOGY:Straight single-rooted teeth with mature apices were divided into 10 groups of 75 teeth and one gr...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.2002.00481.x

    authors: De Moor RJ,Hommez GM

    更新日期:2002-03-01 00:00:00

  • Root canal morphology of mandibular first premolars in an Indian population: a laboratory study.

    abstract:AIM:To determine the root canal morphology of mandibular first premolar teeth in an Indian population using a decalcification and clearing technique. METHODOLOGY:One hundred extracted adult mandibular first premolar teeth were studied following decalcification and clearing. The shape of the canal orifice, root canal p...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2008.01494.x

    authors: Velmurugan N,Sandhya R

    更新日期:2009-01-01 00:00:00