The preparation of periapical lesions for flow cytometry.

Abstract:

AIM:To devise an optimal protocol and to analyse the leucocyte composition of periapical (PA) lesions by flow cytometry. METHODOLOGY:PA lesions were mechanically agitated, with and without proteolysis. This was with either 0.2% collagenase alone, or in combination with 0.02% DNA-ase in serial incubations until all tissue was digested. The efficacy of each method was assessed by counting total cell yield and cell viability. Phenotype stability was gauged by the percentage of peripheral blood leucocytes (PBL) which expressed CD45RB, CD3, CD20, CD4 and CD8 before and after mechanical and collagenase treatment. RESULTS:Disaggregation of PA lesions was superior if collagenase was present, but cell clumping was problematic unless the DNA-ase was also added, and serial digestion with this combination produced optimal cell yield and viability. Nevertheless, the total number of cells released rarely exceeded 105, though viability was in excess of 80%. Mechanical agitation and proteolysis adversely affected PBL phenotypes, but collagenase digestion limited to 10 min caused least damage. Flow cytometric analysis of disaggregated PA lesions failed to identify more than 7.9% (mean, range 6-10%) CD45RB + cells. CONCLUSIONS:Because of the necessity for single cell suspensions, flow cytometry is not easily applied to the analysis of leucocytes in PA lesions, and further refinements in tissue disaggregation and cell preparation are required.

journal_name

Int Endod J

authors

Fernando K,Gulabivala K,Barrett AW

doi

10.1046/j.1365-2591.2000.00294.x

keywords:

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

103-12

issue

2

eissn

0143-2885

issn

1365-2591

journal_volume

33

pub_type

杂志文章
  • Incidence and antimicrobial susceptibility of Porphyromonas gingivalis isolated from mixed endodontic infections.

    abstract:AIM:To investigate the prevalence of Porphyromonas gingivalis in root canals of infected teeth with periapical abscesses and to investigate the antimicrobial susceptibility of this species to some frequently prescribed antibiotics. METHODOLOGY:Samples were obtained from 70 root canals of abscessed teeth. Microbial sam...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2005.01053.x

    authors: Jacinto RC,Gomes BP,Shah HN,Ferraz CC,Zaia AA,Souza-Filho FJ

    更新日期:2006-01-01 00:00:00

  • Fracture resistance of root filled premolar teeth restored with direct composite resin with or without cusp coverage.

    abstract:AIM:To evaluate the fracture resistance and fracture patterns of root filled maxillary first premolar teeth (with mesio-occlusal cavity preparation) restored with several composite restoration designs. METHODOLOGY:One hundred extracted sound human maxillary first premolars were randomly divided into five groups. Group...

    journal_title:International endodontic journal

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1365-2591.2011.02005.x

    authors: Xie KX,Wang XY,Gao XJ,Yuan CY,Li JX,Chu CH

    更新日期:2012-06-01 00:00:00

  • Effect of delayed scanning on imaging and on the diagnostic accuracy of vertical root fractures in two photostimulable phosphor plates digital systems.

    abstract:AIM:To evaluate the influence of delayed scanning on images obtained with two PSPs digital systems and on the diagnostic accuracy of vertical root fracture (VRF) by means of objective and subjective analyses. METHODOLOGY:Forty single-rooted human teeth were divided into two groups, one without VRFs and another with VR...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12547

    authors: Nascimento HA,Visconti MA,Ferreira LM,Suarez MA,Haiter Neto F,Freitas DQ

    更新日期:2016-10-01 00:00:00

  • Influence of cusp coverage on the fracture resistance of premolars with endodontic access cavities.

    abstract:AIM:To assess the influence of cusp reduction and coverage with composite resin on the fracture resistance of premolars with prepared access cavities. METHODOLOGY:Endodontic access cavities were prepared in 60 premolar teeth that were divided into four test groups: R1, R2, R3 and NR (n=15). In all test groups, MOD cav...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2011.01859.x

    authors: ElAyouti A,Serry MI,Geis-Gerstorfer J,Löst C

    更新日期:2011-06-01 00:00:00

  • Second-generation platelet concentrate (PRF) as a pulpotomy medicament in a permanent molar with pulpitis: a case report.

    abstract:AIM:  To discuss the clinical and radiographic success of a pulpotomy with second-generation platelet concentrate (PRF), in a human mature permanent molar tooth. SUMMARY:  A 19-year-old female patient reported to the Department of Conservative Dentistry and Endodontics with established pulpitis in tooth 36. The tooth ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2011.01973.x

    authors: Hiremath H,Saikalyan S,Kulkarni SS,Hiremath V

    更新日期:2012-01-01 00:00:00

  • Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein-Barr virus.

    abstract:AIM:To investigate whether apical periodontitis lesions infected by Epstein-Barr virus (EBV) exhibit higher levels of oxidative stress biomarkers [8-hydroxydeoxyguanosine (8-OHdG) and oxidized glutathione (GSSG)] and bone resorption regulators [receptor activator of nuclear factor (NF-κB) ligand (RANKL) and osteoproteg...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12886

    authors: Jakovljevic A,Andric M,Nikolic N,Coric V,Krezovic S,Carkic J,Knezevic A,Beljic-Ivanovic K,Pljesa-Ercegovac M,Miletic M,Soldatovic I,Radosavljevic T,Jovanovic T,Simic T,Ivanovic V,Milasin J

    更新日期:2018-06-01 00:00:00

  • Evaluation of the effect of EDTA, EDTAC and citric acid on the microhardness of root dentine.

    abstract:AIM:To evaluate the effect of citric acid, ethylenediaminetetraacetic acid (EDTA) and ethylenediaminetetraacetic acid plus Cetavlon (EDTAC) solutions on the microhardness of human root canal dentine. METHODOLOGY:Sixteen maxillary human canines were sectioned transversely at the cemento-enamel junction and the crowns w...

    journal_title:International endodontic journal

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1365-2591.2006.01094.x

    authors: De-Deus G,Paciornik S,Mauricio MH

    更新日期:2006-05-01 00:00:00

  • A maxillary lateral incisor with four root canals.

    abstract:AIM:To describe the successful non-surgical endodontic management of a permanent maxillary lateral incisor tooth with four root canals. SUMMARY:Aberrant morphology of root canals can occur in any tooth, including maxillary lateral incisors. In the presented case, clinical examination and radiographs clearly depicted t...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2011.01984.x

    authors: Kottoor J,Murugesan R,Albuquerque DV

    更新日期:2012-04-01 00:00:00

  • Shaping ability of the M4 handpiece and Safety Hedstrom files in simulated root canals.

    abstract::The aim of this study was to assess the shaping ability of the M4 reciprocating handpiece and Safety Hedstrom files in simulated canals. A total of 40 simulated canals of various angles and positions of curvature were prepared with an M4 handpiece using Safety Hedstrom files oriented with the ground, flattened surface...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.1997.tb01094.x

    authors: Lloyd A,Jaunberzins A,Dhopatkar A,Bryant S,Dummer PM

    更新日期:1997-01-01 00:00:00

  • Radiopacity of root filling materials using digital radiography.

    abstract:AIM:To evaluate radiopacity of root filling materials using digital radiography. METHODOLOGY:The sealers tested were AH Plus, Endofill, EndoREZ and Epiphany. Gutta-percha (Dentsply Maillefer) and Resilon cones were also tested. Acrylic plates, containing six wells, measuring 1 mm in depth and 5 mm in diameter, were pr...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2007.01246.x

    authors: Carvalho-Junior JR,Correr-Sobrinho L,Correr AB,Sinhoreti MA,Consani S,Sousa-Neto MD

    更新日期:2007-07-01 00:00:00

  • Vertical root fractures in adjacent maxillary premolars: an endodontic-prosthetic perplexity.

    abstract::Vertical root fractures in endodontically treated teeth occur frequently in teeth or roots in which their mesiodistal dimension is narrow, such as the maxillary upper premolars. Two cases of vertical root fractures in two adjacent maxillary reconstructed teeth are presented. As in many cases of vertical root fractures...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.1998.00129.x

    authors: Tamse A,Zilburg I,Halpern J

    更新日期:1998-03-01 00:00:00

  • An in-vitro investigation of the antibacterial effect of nisin in root canals and canal wall radicular dentine.

    abstract:AIM:To determine whether nisin, a bacteriocin, would be effective at killing Enterococcus faecalis and Streptococcus gordonii cells in solution and within the root canal system. METHODOLOGY:Bacterial isolates of E. faecalis and S. gordonii were grown from glycerol stocks in closed tubes containing BHY broth at 37 degr...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2004.00846.x

    authors: Turner SR,Love RM,Lyons KM

    更新日期:2004-10-01 00:00:00

  • Microflora in teeth associated with apical periodontitis: a methodological observational study comparing two protocols and three microscopy techniques.

    abstract:AIM:The aim of this study was to compare two protocols to examine bacterial colonization in teeth associated with chronic apical periodontitis with acute episodes (ap), using light microscopy (LM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). METHODOLOGY:Nine root samples (seven teeth...

    journal_title:International endodontic journal

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1365-2591.2009.01594.x

    authors: Richardson N,Mordan NJ,Figueiredo JA,Ng YL,Gulabivala K

    更新日期:2009-10-01 00:00:00

  • Coronal restoration of the root filled tooth - a qualitative analysis of the dentists' decision-making process.

    abstract:AIM:To describe the decision-making process of the general dental practitioner (GDP) underlying the choice of coronal restoration of a root filled tooth. METHODOLOGY:GDPs were strategically selected with respect to gender, age, undergraduate dental school, service affiliation and duration of professional experience. S...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.13442

    authors: Dawson VS,Fransson H,Wolf E

    更新日期:2020-11-07 00:00:00

  • Canal configuration of the mesiobuccal root of the maxillary first molar of a Japanese sub-population.

    abstract:AIM:The aim of this study was to determine the percentage of anatomical canal configurations of the mesiobuccal root of the maxillary first molar in Japanese patients. METHODOLOGY:Three hundred teeth were obtained from general dentists who knew absolutely that they were extracted from Japanese patients. The distobucca...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.1999.00186.x

    authors: Weine FS,Hayami S,Hata G,Toda T

    更新日期:1999-03-01 00:00:00

  • Radiographic evaluation of cases referred for surgical endodontics.

    abstract:AIM:The aim of this study is to test the hypothesis that more patients with failed root-canal treatment or other endodontic problems are referred for periradicular surgery rather than nonsurgical re-treatment. METHODOLOGY:Three sets of 100 periapical radiographs representing typical cases referred for surgical treatme...

    journal_title:International endodontic journal

    pub_type: 杂志文章,多中心研究

    doi:10.1046/j.1365-2591.2002.00510.x

    authors: Doornbusch H,Broersma L,Boering G,Wesselink PR

    更新日期:2002-05-01 00:00:00

  • Chelating agents in root canal treatment: mode of action and indications for their use.

    abstract::Chelating agents were introduced into endodontics as an aid for the preparation of narrow and calcified root canals in 1957 by Nygaard-Østby. A liquid solution of ethylenediaminetetraacetic acid (EDTA) was thought to chemically soften the root canal dentine and dissolve the smear layer, as well as to increase dentine ...

    journal_title:International endodontic journal

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2591.2003.00754.x

    authors: Hülsmann M,Heckendorff M,Lennon A

    更新日期:2003-12-01 00:00:00

  • Effects of ultrasonic removal of fractured files from the middle third of root canals on dentinal cracks: a micro-computed tomography study.

    abstract:AIM:To evaluate the frequency of dentinal microcracks after ultrasonic removal of fractured files from the middle third of root canals using micro-computed tomography (micro-CT). METHODOLOGY:Eighteen bilaterally matched pairs of human mandibular incisors extracted for periodontal reasons were included. The matched pai...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12909

    authors: Fu M,Huang X,He W,Hou B

    更新日期:2018-09-01 00:00:00

  • Behaviour of human dental pulp cells cultured in a collagen hydrogel scaffold cross-linked with cinnamaldehyde.

    abstract:AIM:To investigate the effects of the cross-linking agent cinnamaldehyde (CA) on differentiation of human dental pulp cells (hDPCs) cultured in a collagen hydrogel, which may be useful as a scaffold for regenerative endodontic therapy. METHODOLOGY:The odontogenic potential of hDPCs exposed to CA was examined using alk...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12592

    authors: Kwon YS,Lee SH,Hwang YC,Rosa V,Lee KW,Min KS

    更新日期:2017-01-01 00:00:00

  • Percutaneous injuries amongst Greek endodontists: a national questionnaire survey.

    abstract:AIM:To investigate amongst Greek endodontists the incidence of percutaneous injuries, the circumstances associated with them, the therapeutic measures taken after the injuries and their compliance with infection control measures. METHODOLOGY:One hundred and forty-seven endodontists met the inclusion criteria and were ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2012.02126.x

    authors: Zarra T,Lambrianidis T

    更新日期:2013-03-01 00:00:00

  • Efficacy of a sustained-release device containing chlorhexidine and Ca(OH)2 in preventing secondary infection of dentinal tubules.

    abstract::This study was conducted to evaluate the efficacy of the antibacterial activity of Ca(OH)2 and a sustained-release device containing chlorhexidine (SRD) in both sterilization and prevention of secondary infection of the root canal system. Bovine root dentine specimens previously incubated with Streptococcus faecalis w...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.1992.tb00944.x

    authors: Heling I,Steinberg D,Kenig S,Gavrilovich I,Sela MN,Friedman M

    更新日期:1992-01-01 00:00:00

  • Periapical changes following root-canal treatment observed 20-27 years postoperatively.

    abstract:AIM:The aim of the present study was to identify periapical changes 20-27 years after root-canal treatment. METHODOLOGY:The periapical condition of 265 roots filled by undergraduate students was evaluated in two series of intraoral radiographs taken 10-17 and 20-27 years after treatment. Roots (72) not recorded with a...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.2002.00568.x

    authors: Molven O,Halse A,Fristad I,MacDonald-Jankowski D

    更新日期:2002-09-01 00:00:00

  • Biocompatibility of dental materials used in contemporary endodontic therapy: a review. Part 2. Root-canal-filling materials.

    abstract::Root-canal-filling materials are either placed directly onto vital periapical tissues or may leach through dentine. The tissue response to these materials therefore becomes important and may influence the outcome of endodontic treatment. This paper is a review of the biocompatibility of contemporary orthograde and ret...

    journal_title:International endodontic journal

    pub_type: 杂志文章,评审

    doi:10.1046/j.1365-2591.2003.00637.x

    authors: Hauman CH,Love RM

    更新日期:2003-03-01 00:00:00

  • Activated platelets increase proliferation and protein synthesis of human dental pulp-derived cells.

    abstract:AIM:To evaluate the impact of activated platelets on the mitogenic expansion of human dental pulp-derived cells (DPC) in vitro. METHODOLOGY:The effect of supernatants released from activated platelets (PRS) and the corresponding platelet membranes (MEM) on proliferation and protein synthesis of DPC was evaluated. The ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2009.01650.x

    authors: Agis H,Stampfl B,Watzek G,Gruber R

    更新日期:2010-02-01 00:00:00

  • Osteogenic cell response to calcium aluminate-based cement.

    abstract:AIM:To evaluate the effect of a calcium aluminate-based cement (CAC+) on the development of the osteogenic phenotype in vitro. METHODOLOGY:Rat calvaria-derived cells were grown on Thermanox® coverslips for 24 h and then exposed to either samples (4-h set) of CAC+ or mineral trioxide aggregate (MTA) placed on Transwell...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12682

    authors: Castro-Raucci LMS,Teixeira LN,Oliveira IR,Raucci-Neto W,Jacobovitz M,Rosa AL,de Oliveira PT

    更新日期:2017-08-01 00:00:00

  • The potential of a surface pre-reacted glass root canal dressing for treating apical periodontitis in rats.

    abstract:AIM:To evaluate the efficacy of a prototype root canal dressing containing surface pre-reacted glass-ionomer (S-PRG) fillers on repairing induced periapical lesions in a rat model. Calcium hydroxide [Ca(OH)2 ] was applied as a comparison in the healing process. METHODOLOGY:The pulp chambers of the maxillary first mola...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.13414

    authors: Xiong B,Shirai K,Matsumoto K,Abiko Y,Furuichi Y

    更新日期:2021-02-01 00:00:00

  • Temperature and surface changes of dentine and cementum induced by CO2 laser exposure.

    abstract::The thermal effects of a CO2 laser on the external root surface and inside the root canal were studied in vitro by means of computerized infrared (IR) thermography and a digital thermometer. One-hundred-and-eighty tooth roots with single root canals were irradiated internally and externally with laser power set at 0.5...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.1993.tb00573.x

    authors: Anić I,Dzubur A,Vidović D,Tudja M

    更新日期:1993-09-01 00:00:00

  • Quality guidelines for endodontic treatment: consensus report of the European Society of Endodontology.

    abstract::The assurance of the quality of a service rendered by a member of the dental profession is an essential feature of any system of peer review in dentistry. This document addresses two essential elements: (i) appropriateness of treatment modality and (ii) quality or level of treatment rendered. In revising these guideli...

    journal_title:International endodontic journal

    pub_type: 共识发展会议,杂志文章,实务指引

    doi:10.1111/j.1365-2591.2006.01180.x

    authors: European Society of Endodontology.

    更新日期:2006-12-01 00:00:00

  • Effect of sodium hypochlorite on mechanical properties of dentine and tooth surface strain.

    abstract:AIM:The aim of this study was to test the null hypothesis that sodium hypochlorite irrigation of root canals does not alter the properties of dentine and contribute to the weakening of root-treated teeth. METHODOLOGY:The effect of two concentrations (0.5%, 5.25%) of sodium hypochlorite (NaOCl) and saline on (i) the el...

    journal_title:International endodontic journal

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1046/j.1365-2591.2001.00357.x

    authors: Sim TP,Knowles JC,Ng YL,Shelton J,Gulabivala K

    更新日期:2001-03-01 00:00:00

  • Endodontic treatment of primary teeth using a combination of antibacterial drugs.

    abstract:AIM:To evaluate the clinical outcome of 'Lesion Sterilization and Tissue Repair' (LSTR) therapy for endodontic treatment of primary teeth. METHODOLOGY:A mixture of metronidazole, ciprofloxacin, and minocycline (3Mix) in ointment (macrogol mixed with propylene glycol: MP) or in a root canal sealer was used to disinfect...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.0143-2885.2004.00771.x

    authors: Takushige T,Cruz EV,Asgor Moral A,Hoshino E

    更新日期:2004-02-01 00:00:00