Abstract:
AIM:To devise an optimal protocol and to analyse the leucocyte composition of periapical (PA) lesions by flow cytometry. METHODOLOGY:PA lesions were mechanically agitated, with and without proteolysis. This was with either 0.2% collagenase alone, or in combination with 0.02% DNA-ase in serial incubations until all tissue was digested. The efficacy of each method was assessed by counting total cell yield and cell viability. Phenotype stability was gauged by the percentage of peripheral blood leucocytes (PBL) which expressed CD45RB, CD3, CD20, CD4 and CD8 before and after mechanical and collagenase treatment. RESULTS:Disaggregation of PA lesions was superior if collagenase was present, but cell clumping was problematic unless the DNA-ase was also added, and serial digestion with this combination produced optimal cell yield and viability. Nevertheless, the total number of cells released rarely exceeded 105, though viability was in excess of 80%. Mechanical agitation and proteolysis adversely affected PBL phenotypes, but collagenase digestion limited to 10 min caused least damage. Flow cytometric analysis of disaggregated PA lesions failed to identify more than 7.9% (mean, range 6-10%) CD45RB + cells. CONCLUSIONS:Because of the necessity for single cell suspensions, flow cytometry is not easily applied to the analysis of leucocytes in PA lesions, and further refinements in tissue disaggregation and cell preparation are required.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Fernando K,Gulabivala K,Barrett AWdoi
10.1046/j.1365-2591.2000.00294.xkeywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
103-12issue
2eissn
0143-2885issn
1365-2591journal_volume
33pub_type
杂志文章abstract:AIM:To investigate the prevalence of Porphyromonas gingivalis in root canals of infected teeth with periapical abscesses and to investigate the antimicrobial susceptibility of this species to some frequently prescribed antibiotics. METHODOLOGY:Samples were obtained from 70 root canals of abscessed teeth. Microbial sam...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2005.01053.x
更新日期:2006-01-01 00:00:00
abstract:AIM:To evaluate the fracture resistance and fracture patterns of root filled maxillary first premolar teeth (with mesio-occlusal cavity preparation) restored with several composite restoration designs. METHODOLOGY:One hundred extracted sound human maxillary first premolars were randomly divided into five groups. Group...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2011.02005.x
更新日期:2012-06-01 00:00:00
abstract:AIM:To evaluate the influence of delayed scanning on images obtained with two PSPs digital systems and on the diagnostic accuracy of vertical root fracture (VRF) by means of objective and subjective analyses. METHODOLOGY:Forty single-rooted human teeth were divided into two groups, one without VRFs and another with VR...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12547
更新日期:2016-10-01 00:00:00
abstract:AIM:To assess the influence of cusp reduction and coverage with composite resin on the fracture resistance of premolars with prepared access cavities. METHODOLOGY:Endodontic access cavities were prepared in 60 premolar teeth that were divided into four test groups: R1, R2, R3 and NR (n=15). In all test groups, MOD cav...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01859.x
更新日期:2011-06-01 00:00:00
abstract:AIM: To discuss the clinical and radiographic success of a pulpotomy with second-generation platelet concentrate (PRF), in a human mature permanent molar tooth. SUMMARY: A 19-year-old female patient reported to the Department of Conservative Dentistry and Endodontics with established pulpitis in tooth 36. The tooth ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01973.x
更新日期:2012-01-01 00:00:00
abstract:AIM:To investigate whether apical periodontitis lesions infected by Epstein-Barr virus (EBV) exhibit higher levels of oxidative stress biomarkers [8-hydroxydeoxyguanosine (8-OHdG) and oxidized glutathione (GSSG)] and bone resorption regulators [receptor activator of nuclear factor (NF-κB) ligand (RANKL) and osteoproteg...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12886
更新日期:2018-06-01 00:00:00
abstract:AIM:To evaluate the effect of citric acid, ethylenediaminetetraacetic acid (EDTA) and ethylenediaminetetraacetic acid plus Cetavlon (EDTAC) solutions on the microhardness of human root canal dentine. METHODOLOGY:Sixteen maxillary human canines were sectioned transversely at the cemento-enamel junction and the crowns w...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2006.01094.x
更新日期:2006-05-01 00:00:00
abstract:AIM:To describe the successful non-surgical endodontic management of a permanent maxillary lateral incisor tooth with four root canals. SUMMARY:Aberrant morphology of root canals can occur in any tooth, including maxillary lateral incisors. In the presented case, clinical examination and radiographs clearly depicted t...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01984.x
更新日期:2012-04-01 00:00:00
abstract::The aim of this study was to assess the shaping ability of the M4 reciprocating handpiece and Safety Hedstrom files in simulated canals. A total of 40 simulated canals of various angles and positions of curvature were prepared with an M4 handpiece using Safety Hedstrom files oriented with the ground, flattened surface...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1997.tb01094.x
更新日期:1997-01-01 00:00:00
abstract:AIM:To evaluate radiopacity of root filling materials using digital radiography. METHODOLOGY:The sealers tested were AH Plus, Endofill, EndoREZ and Epiphany. Gutta-percha (Dentsply Maillefer) and Resilon cones were also tested. Acrylic plates, containing six wells, measuring 1 mm in depth and 5 mm in diameter, were pr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01246.x
更新日期:2007-07-01 00:00:00
abstract::Vertical root fractures in endodontically treated teeth occur frequently in teeth or roots in which their mesiodistal dimension is narrow, such as the maxillary upper premolars. Two cases of vertical root fractures in two adjacent maxillary reconstructed teeth are presented. As in many cases of vertical root fractures...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1998.00129.x
更新日期:1998-03-01 00:00:00
abstract:AIM:To determine whether nisin, a bacteriocin, would be effective at killing Enterococcus faecalis and Streptococcus gordonii cells in solution and within the root canal system. METHODOLOGY:Bacterial isolates of E. faecalis and S. gordonii were grown from glycerol stocks in closed tubes containing BHY broth at 37 degr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00846.x
更新日期:2004-10-01 00:00:00
abstract:AIM:The aim of this study was to compare two protocols to examine bacterial colonization in teeth associated with chronic apical periodontitis with acute episodes (ap), using light microscopy (LM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). METHODOLOGY:Nine root samples (seven teeth...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2009.01594.x
更新日期:2009-10-01 00:00:00
abstract:AIM:To describe the decision-making process of the general dental practitioner (GDP) underlying the choice of coronal restoration of a root filled tooth. METHODOLOGY:GDPs were strategically selected with respect to gender, age, undergraduate dental school, service affiliation and duration of professional experience. S...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13442
更新日期:2020-11-07 00:00:00
abstract:AIM:The aim of this study was to determine the percentage of anatomical canal configurations of the mesiobuccal root of the maxillary first molar in Japanese patients. METHODOLOGY:Three hundred teeth were obtained from general dentists who knew absolutely that they were extracted from Japanese patients. The distobucca...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00186.x
更新日期:1999-03-01 00:00:00
abstract:AIM:The aim of this study is to test the hypothesis that more patients with failed root-canal treatment or other endodontic problems are referred for periradicular surgery rather than nonsurgical re-treatment. METHODOLOGY:Three sets of 100 periapical radiographs representing typical cases referred for surgical treatme...
journal_title:International endodontic journal
pub_type: 杂志文章,多中心研究
doi:10.1046/j.1365-2591.2002.00510.x
更新日期:2002-05-01 00:00:00
abstract::Chelating agents were introduced into endodontics as an aid for the preparation of narrow and calcified root canals in 1957 by Nygaard-Østby. A liquid solution of ethylenediaminetetraacetic acid (EDTA) was thought to chemically soften the root canal dentine and dissolve the smear layer, as well as to increase dentine ...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2591.2003.00754.x
更新日期:2003-12-01 00:00:00
abstract:AIM:To evaluate the frequency of dentinal microcracks after ultrasonic removal of fractured files from the middle third of root canals using micro-computed tomography (micro-CT). METHODOLOGY:Eighteen bilaterally matched pairs of human mandibular incisors extracted for periodontal reasons were included. The matched pai...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12909
更新日期:2018-09-01 00:00:00
abstract:AIM:To investigate the effects of the cross-linking agent cinnamaldehyde (CA) on differentiation of human dental pulp cells (hDPCs) cultured in a collagen hydrogel, which may be useful as a scaffold for regenerative endodontic therapy. METHODOLOGY:The odontogenic potential of hDPCs exposed to CA was examined using alk...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12592
更新日期:2017-01-01 00:00:00
abstract:AIM:To investigate amongst Greek endodontists the incidence of percutaneous injuries, the circumstances associated with them, the therapeutic measures taken after the injuries and their compliance with infection control measures. METHODOLOGY:One hundred and forty-seven endodontists met the inclusion criteria and were ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2012.02126.x
更新日期:2013-03-01 00:00:00
abstract::This study was conducted to evaluate the efficacy of the antibacterial activity of Ca(OH)2 and a sustained-release device containing chlorhexidine (SRD) in both sterilization and prevention of secondary infection of the root canal system. Bovine root dentine specimens previously incubated with Streptococcus faecalis w...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1992.tb00944.x
更新日期:1992-01-01 00:00:00
abstract:AIM:The aim of the present study was to identify periapical changes 20-27 years after root-canal treatment. METHODOLOGY:The periapical condition of 265 roots filled by undergraduate students was evaluated in two series of intraoral radiographs taken 10-17 and 20-27 years after treatment. Roots (72) not recorded with a...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00568.x
更新日期:2002-09-01 00:00:00
abstract::Root-canal-filling materials are either placed directly onto vital periapical tissues or may leach through dentine. The tissue response to these materials therefore becomes important and may influence the outcome of endodontic treatment. This paper is a review of the biocompatibility of contemporary orthograde and ret...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.2003.00637.x
更新日期:2003-03-01 00:00:00
abstract:AIM:To evaluate the impact of activated platelets on the mitogenic expansion of human dental pulp-derived cells (DPC) in vitro. METHODOLOGY:The effect of supernatants released from activated platelets (PRS) and the corresponding platelet membranes (MEM) on proliferation and protein synthesis of DPC was evaluated. The ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01650.x
更新日期:2010-02-01 00:00:00
abstract:AIM:To evaluate the effect of a calcium aluminate-based cement (CAC+) on the development of the osteogenic phenotype in vitro. METHODOLOGY:Rat calvaria-derived cells were grown on Thermanox® coverslips for 24 h and then exposed to either samples (4-h set) of CAC+ or mineral trioxide aggregate (MTA) placed on Transwell...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12682
更新日期:2017-08-01 00:00:00
abstract:AIM:To evaluate the efficacy of a prototype root canal dressing containing surface pre-reacted glass-ionomer (S-PRG) fillers on repairing induced periapical lesions in a rat model. Calcium hydroxide [Ca(OH)2 ] was applied as a comparison in the healing process. METHODOLOGY:The pulp chambers of the maxillary first mola...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13414
更新日期:2021-02-01 00:00:00
abstract::The thermal effects of a CO2 laser on the external root surface and inside the root canal were studied in vitro by means of computerized infrared (IR) thermography and a digital thermometer. One-hundred-and-eighty tooth roots with single root canals were irradiated internally and externally with laser power set at 0.5...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1993.tb00573.x
更新日期:1993-09-01 00:00:00
abstract::The assurance of the quality of a service rendered by a member of the dental profession is an essential feature of any system of peer review in dentistry. This document addresses two essential elements: (i) appropriateness of treatment modality and (ii) quality or level of treatment rendered. In revising these guideli...
journal_title:International endodontic journal
pub_type: 共识发展会议,杂志文章,实务指引
doi:10.1111/j.1365-2591.2006.01180.x
更新日期:2006-12-01 00:00:00
abstract:AIM:The aim of this study was to test the null hypothesis that sodium hypochlorite irrigation of root canals does not alter the properties of dentine and contribute to the weakening of root-treated teeth. METHODOLOGY:The effect of two concentrations (0.5%, 5.25%) of sodium hypochlorite (NaOCl) and saline on (i) the el...
journal_title:International endodontic journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-2591.2001.00357.x
更新日期:2001-03-01 00:00:00
abstract:AIM:To evaluate the clinical outcome of 'Lesion Sterilization and Tissue Repair' (LSTR) therapy for endodontic treatment of primary teeth. METHODOLOGY:A mixture of metronidazole, ciprofloxacin, and minocycline (3Mix) in ointment (macrogol mixed with propylene glycol: MP) or in a root canal sealer was used to disinfect...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.0143-2885.2004.00771.x
更新日期:2004-02-01 00:00:00