Abstract:
INTRODUCTION:Accurate preclinical modeling of diabetic complications such as retinopathy, nephropathy and neuropathy is crucial to enable the development of novel preventative therapies. The aims of this study were to establish a model of long-term diabetes with sustained medium scale hyperglycemia and characterize the pathological changes detectable after 4months, with particular respect to dependence on the degree of hyperglycemia. METHODS:Streptozotocin-induced diabetic CFY rats were subjected to four different insulin substitution protocols to achieve different levels of glycemic control (Diabetic 1-4 groups). Eyes were investigated by ophthalmoscopy, kidney function by urine analysis, and neuropathy by functional tests. Retinal and renal morphological evaluations were performed by histology, immuno-histochemistry and electron microscopy. RESULTS:Rats of the Diabetic 3 group showed massive hyperglycemia-dependent anterior segment neovascularization, enhanced total retinal score and retinal apoptotic cell number, degeneration of dopaminergic amacrine cells, increased glomerular PAS-positivity, altered excreted total protein/creatinine ratio and cold allodynia, parallel with medium scale hyperglycemia (blood glucose level between 22 and 25mmol/L) and satisfying state of health. DISCUSSION:We established a treatment protocol in rats enabling complex investigation of diabetic retinopathy, nephropathy and neuropathy on a long-term period. Clearly hyperglycemic dependent parameters of these complications serve as good outcome measures for preclinical trials. Our results provide a useful basis for designing studies for testing preventative treatments as well as other translational medical research in this field.
journal_name
J Pharmacol Toxicol Methodsjournal_title
Journal of pharmacological and toxicological methodsauthors
Hajna Z,Szabadfi K,Balla Z,Biró Z,Degrell P,Molnár GA,Kőszegi T,Tékus V,Helyes Z,Dobos A,Farkas S,Szűcs G,Gábriel R,Pintér Edoi
10.1016/j.vascn.2015.11.003subject
Has Abstractpub_date
2016-03-01 00:00:00pages
1-12eissn
1056-8719issn
1873-488Xpii
S1056-8719(15)30001-0journal_volume
78pub_type
杂志文章abstract::Induction of acute myocardial infarction in the rat is an established model for studying effects of therapeutic interventions. Images of sections of the rat left ventricle, stained with nitroblue tetrazolium, were digitized and several parameters estimated by dedicated software on an image analyzer (IBAS 2.0). The met...
journal_title:Journal of pharmacological and toxicological methods
pub_type: 杂志文章
doi:10.1016/1056-8719(95)00019-e
更新日期:1995-08-01 00:00:00
abstract::High-content screening (HCS) systems can be used for high-throughput screening of drugs in human embryonic stem cells (hESCs). However, hESCs require immunofluorescence staining with stemness markers (e.g., Oct-4) prior to HCS, which can be time consuming and labor intensive. In this study, we employed transgenic hESC...
journal_title:Journal of pharmacological and toxicological methods
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journal_title:Journal of pharmacological and toxicological methods
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journal_title:Journal of pharmacological and toxicological methods
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abstract::Evaluation of angiotensin II (AII) receptor binding often necessitates freezing of the tissue of interest prior to assay of radioligand binding. This study evaluated the effects of freezing of various rat tissues at different rates on 125I-sarcosine1, isoleucine8 angiotensin II (125I-SI AII) binding to AII receptor su...
journal_title:Journal of pharmacological and toxicological methods
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journal_title:Journal of pharmacological and toxicological methods
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abstract:INTRODUCTION:We characterised the development of Type 2 diabetes and associated changes in islet appearance in female ZDF rats and explored its suitability for studies with novel therapeutic agents. METHODS:Female ZDF rats were either chow or high fat (60%) fed for up to 36 days and blood glucose and plasma insulin co...
journal_title:Journal of pharmacological and toxicological methods
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journal_title:Journal of pharmacological and toxicological methods
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journal_title:Journal of pharmacological and toxicological methods
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journal_title:Journal of pharmacological and toxicological methods
pub_type: 杂志文章
doi:10.1016/j.vascn.2004.02.001
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journal_title:Journal of pharmacological and toxicological methods
pub_type: 杂志文章
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journal_title:Journal of pharmacological and toxicological methods
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更新日期:2020-09-01 00:00:00
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journal_title:Journal of pharmacological and toxicological methods
pub_type: 杂志文章
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journal_title:Journal of pharmacological and toxicological methods
pub_type: 杂志文章
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journal_title:Journal of pharmacological and toxicological methods
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doi:10.1016/j.vascn.2004.08.012
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journal_title:Journal of pharmacological and toxicological methods
pub_type: 杂志文章
doi:10.1016/j.vascn.2008.03.001
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abstract:INTRODUCTION:The evaluation of drug's cytotoxicity is a crucial step in the development of new pharmacological compounds. 31P NMR can be a tool for toxicological screening, as it enables the study of drugs' cytotoxicity and their effect on cell energy metabolism in a real-time, in a non- invasive and non-destructive wa...
journal_title:Journal of pharmacological and toxicological methods
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journal_title:Journal of pharmacological and toxicological methods
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journal_title:Journal of pharmacological and toxicological methods
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journal_title:Journal of pharmacological and toxicological methods
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journal_title:Journal of pharmacological and toxicological methods
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abstract:INTRODUCTION:Dipeptidyl peptidases (DPPs) 8 and 9 are homologous, cytoplasmic postproline-cutting enzymes, which have similar enzymatic activity and preferred substrates as DPP4. DPP4 is a well-known target for treating diabetes mellitus. With the increased concern of non-selectivity and toxicities caused by DPP4 inhib...
journal_title:Journal of pharmacological and toxicological methods
pub_type: 杂志文章
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journal_title:Journal of pharmacological and toxicological methods
pub_type: 杂志文章
doi:10.1016/j.vascn.2005.03.002
更新日期:2005-09-01 00:00:00
abstract::Fluorescent labeling of amine functional groups using dansyl chloride (DNS-Cl), and in sodium carbonate buffer, allowed the detection of 1 microg amounts of analytes. The methodology presented allows dansylation of primary, secondary, and tertiary amine groups at a temperature of 25 degrees C. The dansylation of terti...
journal_title:Journal of pharmacological and toxicological methods
pub_type: 杂志文章
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abstract:INTRODUCTION:Embryonic rat aortic smooth muscle cells, A7r5, have been used extensively as an in vitro vascular smooth muscle cell model. They are usually provided at 11th passage by supplier and generally used before 25th passage. However, the exact passage number (P#) used is not reported in general. METHODS:In this...
journal_title:Journal of pharmacological and toxicological methods
pub_type: 杂志文章
doi:10.1016/j.vascn.2014.03.001
更新日期:2014-07-01 00:00:00
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journal_title:Journal of pharmacological and toxicological methods
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journal_title:Journal of pharmacological and toxicological methods
pub_type: 杂志文章,评审
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更新日期:1992-09-01 00:00:00
abstract::The purpose of this study was to develop a simple and noninvasive method for the quantitative evaluation of neuromuscular blockade in mice. The sciatic nerve in anesthetized animals (etomidate, 50 mg/kg or mephenesin, 250 mg/kg) was stimulated using transcutaneous electrodes; repetitive supramaximal stimulations (3 Hz...
journal_title:Journal of pharmacological and toxicological methods
pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
abstract:BACKGROUND:Animal models of allergy and bronchial hyperresponsiveness (BHR) are useful in researching pulmonary diseases and evaluating drug effects on the airways. Neonatally immunised rabbits exhibit several features of asthma as adults, including early and late airway responses following antigen challenge, oedema an...
journal_title:Journal of pharmacological and toxicological methods
pub_type: 杂志文章
doi:10.1016/j.vascn.2011.08.003
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