Abstract：
INTRODUCTION:The purpose of this study was to optimize carbon tetrachloride-induced hepatotoxicity in the rat with respect to dose, route of injection, and time course. METHODS:Male Wistar albino rats, 4 to 6 weeks old and weighing 130-180 g were used. Hepatotoxicity was evaluated by measuring the activity of serum enzymes (alkaline phosphatase [ALP], alanine aminotransferase [ALT], and aspartate aminotransferase [AST]) as well as serum total bilirubin level. RESULTS:Intraperitoneal injection of carbon tetrachloride (CCl(4)) increased the activity of ALP (from 64.9 to 137.3 U/l), ALT (from 106.6 to 693.1 U/l), and AST (from 113.8 to 693.9 U/l). Plasma bilirubin level increased (from 0.119 to 0.42 mg/dl). In contrast, subcutaneous injection of CCl(4) had no effect on these variables. The optimum intraperitoneal dose of CCl(4) was found to be 2 ml/kg body weight (dissolved in an equal volume of olive oil), and this increased the level of bilirubin and the activity of the three enzymes significantly, without causing death of the animals. Hepatotoxicity was observed within 2 h of intraperitoneal injection of CCl(4) and reached a peak after 24 h. Bilirubin level and serum enzyme activities declined gradually to normal levels by 3 days after CCl(4) injection. CONCLUSION:It is possible to reliably evoke reversible hepatotoxicity in rats by intraperitoneal injection of 2 ml/kg CCl(4).
journal_name
J Pharmacol Toxicol Methodsjournal_title
Journal of pharmacological and toxicological methodsauthors
Janakat S,Al-Merie Hdoi
10.1016/S1056-8719(03)00019-4keywords:
subject
Has Abstractpub_date
2002-07-01 00:00:00pages
41-4issue
1eissn
1056-8719issn
1873-488Xpii
S1056-8719(03)00019-4journal_volume
48pub_type
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journal_title：Journal of pharmacological and toxicological methods
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journal_title：Journal of pharmacological and toxicological methods
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journal_title：Journal of pharmacological and toxicological methods
pub_type： 杂志文章
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journal_title：Journal of pharmacological and toxicological methods
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journal_title：Journal of pharmacological and toxicological methods
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journal_title：Journal of pharmacological and toxicological methods
pub_type： 杂志文章
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