Lactate dehydrogenase-A inhibition induces human glioblastoma multiforme stem cell differentiation and death.


:Therapies that target the signal transduction and metabolic pathways of cancer stem cells (CSCs) are innovative strategies to effectively reduce the recurrence and significantly improve the outcome of glioblastoma multiforme (GBM). CSCs exhibit an increased rate of glycolysis, thus rendering them intrinsically more sensitive to prospective therapeutic strategies based on the inhibition of the glycolytic pathway. The enzyme lactate dehydrogenase-A (LDH-A), which catalyses the interconversion of pyruvate and lactate, is up-regulated in human cancers, including GBM. Although several papers have explored the benefits of targeting cancer metabolism in GBM, the effects of direct LDH-A inhibition in glial tumours have not yet been investigated, particularly in the stem cell subpopulation. Here, two representative LDH-A inhibitors (NHI-1 and NHI-2) were studied in GBM-derived CSCs and compared to differentiated tumour cells. LDH-A inhibition was particularly effective in CSCs isolated from different GBM cell lines, where the two compounds blocked CSC formation and elicited long-lasting effects by triggering both apoptosis and cellular differentiation. These data demonstrate that GBM, particularly the stem cell subpopulation, is sensitive to glycolytic inhibition and shed light on the therapeutic potential of LDH-A inhibitors in this tumour type.


Sci Rep


Scientific reports


Daniele S,Giacomelli C,Zappelli E,Granchi C,Trincavelli ML,Minutolo F,Martini C




Has Abstract


2015-10-23 00:00:00










  • TLR-3 stimulation improves anti-tumor immunity elicited by dendritic cell exosome-based vaccines in a murine model of melanoma.

    abstract::Dendritic cell (DC)-derived exosomes (Dexo) contain the machinery necessary to activate potent antigen-specific immune responses. As promising cell-free immunogens, Dexo have been tested in previous clinical trials for cancer vaccine immunotherapy, yet resulted in limited therapeutic benefit. Here, we explore a novel ...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Damo M,Wilson DS,Simeoni E,Hubbell JA

    更新日期:2015-12-03 00:00:00

  • A change in the aggregation pathway of bovine serum albumin in the presence of arginine and its derivatives.

    abstract::Chemical chaperones including arginine and its derivatives are widely used by biochemists working on the design of agents, which are able to efficiently suppress protein aggregation. To elucidate the mechanisms of anti-aggregation activity of chemical chaperones, methods based on registration of the increment in light...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Borzova VA,Markossian KA,Kleymenov SY,Kurganov BI

    更新日期:2017-06-21 00:00:00

  • Sex-Related Differences in Impact on Safety of Pharmacogenetic Profile for Colon Cancer Patients Treated with FOLFOX-4 or XELOX Adjuvant Chemotherapy.

    abstract::Polymorphisms contribute to inter-individual differences and show a promising predictive role for chemotherapy-related toxicity in colon cancer (CC). TOSCA is a multicentre, randomized, non-inferiority, phase III study conducted in high-risk stage II/stage III CC patients treated with 6 vs 3 months of FOLFOX-4 or XELO...

    journal_title:Scientific reports

    pub_type: 杂志文章,随机对照试验


    authors: Ruzzo A,Graziano F,Galli F,Galli F,Rulli E,Lonardi S,Ronzoni M,Massidda B,Zagonel V,Pella N,Mucciarini C,Labianca R,Ionta MT,Bagaloni I,Veltri E,Sozzi P,Barni S,Ricci V,Foltran L,Nicolini M,Biondi E,Bramati A,

    更新日期:2019-08-08 00:00:00

  • Promotion of a cancer-like phenotype, through chronic exposure to inflammatory cytokines and hypoxia in a bronchial epithelial cell line model.

    abstract::Globally, lung cancer accounts for approximately 20% of all cancer related deaths. Five-year survival is poor and rates have remained unchanged for the past four decades. There is an urgent need to identify markers of lung carcinogenesis and new targets for therapy. Given the recent successes of immune modulators in c...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Baird AM,Gray SG,Richard DJ,O'Byrne KJ

    更新日期:2016-01-13 00:00:00

  • Experimental demonstration of plasmon enhanced energy transfer rate in NaYF4:Yb(3+),Er(3+) upconversion nanoparticles.

    abstract::Energy transfer upconversion (ETU) is known to be the most efficient frequency upconversion mechanism. Surface plasmon can further enhance the upconversion process, opening doors to many applications. However, ETU is a complex process involving competing transitions between multiple energy levels and it has been diffi...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Lu D,Mao C,Cho SK,Ahn S,Park W

    更新日期:2016-01-07 00:00:00

  • A rare subpopulation of melanoma cells with low expression of metastasis suppressor NME1 is highly metastatic in vivo.

    abstract::Despite recent advances in melanoma treatment, metastasis and resistance to therapy remain serious clinical challenges. NME1 is a metastasis suppressor, a class of proteins which inhibits metastatic spread of cancer cells without impact on growth of the primary tumor. We have identified a rare subpopulation of cells w...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Snyder D,Wang Y,Kaetzel DM

    更新日期:2020-02-06 00:00:00

  • Association of plasma C-reactive protein level with the prevalence of colorectal adenoma: the Colorectal Adenoma Study in Tokyo.

    abstract::Epidemiologic studies have identified a positive association between obesity and colorectal neoplasia. Adiposity induces systemic low-grade inflammation, which is commonly assessed with a sensitive biomarker, C-reactive protein (CRP). To understand the molecular mechanisms of obesity in the etiology of colorectal neop...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Kigawa N,Budhathoki S,Yamaji T,Iwasaki M,Inoue M,Tsugane S

    更新日期:2017-06-30 00:00:00

  • No evidence for spectral jamming avoidance in echolocation behavior of foraging pipistrelle bats.

    abstract::Frequency shifts in signals of bats flying near conspecifics have been interpreted as a spectral jamming avoidance response (JAR). However, several prerequisites supporting a JAR hypothesis have not been controlled for in previous studies. We recorded flight and echolocation behavior of foraging Pipistrellus pipistrel...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Götze S,Koblitz JC,Denzinger A,Schnitzler HU

    更新日期:2016-08-09 00:00:00

  • Exploration of a New Source of Sustainable Nanomaterial from the Koh-e-Suleiman Mountain Range of Pakistan for Industrial Applications.

    abstract::The present study aimed to explore a new source of montmorillonite and to develop an extraction and purification protocol for its isolation from raw clay samples acquired from the Koh-e-Suleiman mountain range in Pakistan. The process involved the collection of raw clay from the source, identification and quantificati...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Nirwan JS,Farhaj S,Chaudhary MM,Khizer Z,Hasan SS,Angelis-Dimakis A,Gill A,Rasheed H,Abbas N,Arshad MS,Hussain T,Shahzad Y,Yousaf AM,Chohan TA,Hussain T,Merchant HA,Akram MR,Khan TM,Ashraf M,Conway BR,Ghori MU

    更新日期:2020-01-17 00:00:00

  • Investigation of thermal energy transport interface of hybrid graphene-carbon nanotube/polyethylene nanocomposites.

    abstract::It is well known the thermal properties of three-dimensional (3-D) hybrid graphene (GR)-carbon nanotube (CNT) structures are not superior to that of the individual GR and CNT, however, the 3-D hybrid GR-CNT structures can effectively improve the thermal properties of polymer matrix. Therefore, understanding the therma...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Liu F,Liu X,Hu N,Ning H,Atobe S,Yan C,Mo F,Fu S,Zhang J,Wang Y,Mu X

    更新日期:2017-10-31 00:00:00

  • Analogue Orientation Control of a Carbon Fibre in a Nematic Liquid Crystal.

    abstract::A carbon fibre is a rod-like microstructure, the longitudinal axis of which is aligned with the orientation of the director in a nematic liquid crystal. A nematic liquid crystal with negative dielectric anisotropy is mixed with carbon fibres. By applying an electric field perpendicular to the director, the carbon fibr...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Lee JY,Lev B,Kim JH

    更新日期:2019-12-27 00:00:00

  • Identification of the earliest collagen- and plant-based coatings from Neolithic artefacts (Nahal Hemar cave, Israel).

    abstract::Mortuary practices in human evolution record cognitive, social changes and technological innovations. The Neolithic Revolution in the Levant was a watershed in this domain that has long fascinated the archaeological community. Plaster modelled skulls are well known at Jericho and several other Neolithic sites, and in ...

    journal_title:Scientific reports

    pub_type: 历史文章,杂志文章


    authors: Solazzo C,Courel B,Connan J,van Dongen BE,Barden H,Penkman K,Taylor S,Demarchi B,Adam P,Schaeffer P,Nissenbaum A,Bar-Yosef O,Buckley M

    更新日期:2016-08-09 00:00:00

  • Determinants of Antibiotic Consumption - Development of a Model using Partial Least Squares Regression based on Data from India.

    abstract::Antibiotic resistance, a consequence of antibiotic use, is a threat to health, with severe consequences for resource constrained settings. If determinants for human antibiotic use in India, a lower middle income country, with one of the highest antibiotic consumption in the world could be understood, interventions cou...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Tamhankar AJ,Karnik SS,Stålsby Lundborg C

    更新日期:2018-04-23 00:00:00

  • Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib.

    abstract::Species generalization in the profound, modality-specific effects of Hedgehog pathway inhibition (HPI) in taste organ homeostasis and sensation is shown. With the HPI, cancer drug sonidegib, we demonstrate that the rat taste system, in addition to mouse, is regulated by Hedgehog signaling. After sonidegib treatment fo...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Kumari A,Yokota Y,Li L,Bradley RM,Mistretta CM

    更新日期:2018-11-01 00:00:00

  • Realizing Broadband and Invertible Linear-to-circular Polarization Converter with Ultrathin Single-layer Metasurface.

    abstract::The arbitrary control of the polarization states of light has attracted the interest of the scientific community because of the wide range of modern optical applications that such control can afford. However, conventional polarization control setups are bulky and very often operate only within a narrow wavelength rang...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Li Z,Liu W,Cheng H,Chen S,Tian J

    更新日期:2015-12-15 00:00:00

  • Structure and nucleic acid binding properties of KOW domains 4 and 6-7 of human transcription elongation factor DSIF.

    abstract::The human transcription elongation factor DSIF is highly conserved throughout all kingdoms of life and plays multiple roles during transcription. DSIF is a heterodimer, consisting of Spt4 and Spt5 that interacts with RNA polymerase II (RNAP II). DSIF binds to the elongation complex and induces promoter-proximal pausin...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Zuber PK,Hahn L,Reinl A,Schweimer K,Knauer SH,Gottesman ME,Rösch P,Wöhrl BM

    更新日期:2018-08-03 00:00:00

  • Ligand-Mediated and Copper-Catalyzed C(sp(3))-H Bond Functionalization of Aryl Ketones with Sodium Sulfinates under Mild Conditions.

    abstract::A novel and convenient copper (II) bromide and 1,8-diazabicyclo[5.4.1]undec-7-ene (DBU) or 1,10-phenanthroline catalysis protocol for the construction of α-alkyl-β-keto sulfones via C(sp(3))-H bond functionalization followed by C(sp(3))-S bond formation between aryl ketones and sodium sulfinates at room temperature ha...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Lan XW,Wang NX,Bai CB,Zhang W,Xing Y,Wen JL,Wang YJ,Li YH

    更新日期:2015-12-18 00:00:00

  • Repercussion of Solid state vs. Liquid state synthesized p-n heterojunction RGO-copper phosphate on proton reduction potential in water.

    abstract::The same copper phosphate catalysts were synthesized by obtaining the methods involving solid state as well as liquid state reactions in this work. And then the optimised p-n hybrid junction photocatalysts have been synthesized following the same solid/liquid reaction pathways. The synthesized copper phosphate photoca...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Samal A,Das DP,Madras G

    更新日期:2018-02-13 00:00:00

  • Targeted mutations in myostatin by zinc-finger nucleases result in double-muscled phenotype in Meishan pigs.

    abstract::Myostatin (MSTN) is a dominant inhibitor of skeletal muscle development and growth. Mutations in MSTN gene can lead to muscle hypertrophy or double-muscled (DM) phenotype in cattle, sheep, dog and human. However, there has not been reported significant muscle phenotypes in pigs in association with MSTN mutations. Pigs...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Qian L,Tang M,Yang J,Wang Q,Cai C,Jiang S,Li H,Jiang K,Gao P,Ma D,Chen Y,An X,Li K,Cui W

    更新日期:2015-09-24 00:00:00

  • Picosecond supercontinuum generation in large mode area photonic crystal fibers for coherent anti-Stokes Raman scattering microspectroscopy.

    abstract::We perform a detailed theoretical and experimental investigation of supercontinuum generation in large-mode-area photonic crystal fibers pumped by a high-energy, high-repetition rate picosecond Nd:YVO4 laser, with the goal of using it as the Stokes beam in coherent anti-Stokes Raman scattering setup. We analyze the in...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Shen Y,Voronin AA,Zheltikov AM,O'Connor SP,Yakovlev VV,Sokolov AV,Scully MO

    更新日期:2018-06-22 00:00:00

  • Disturbance type determines how connectivity shapes ecosystem resilience.

    abstract::Connectivity is fundamentally important for shaping the resilience of complex human and natural networks when systems are disturbed. Ecosystem resilience is, in part, shaped by the spatial arrangement of habitats, the permeability and fluxes between them, the stabilising functions performed by organisms, their dispers...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Pearson RM,Schlacher TA,Jinks KI,Olds AD,Brown CJ,Connolly RM

    更新日期:2021-01-13 00:00:00

  • Cd(II) Sorption on Montmorillonite-Humic acid-Bacteria Composites.

    abstract::Soil components (e.g., clays, bacteria and humic substances) are known to produce mineral-organic composites in natural systems. Herein, batch sorption isotherms, isothermal titration calorimetry (ITC), and Cd K-edge EXAFS spectroscopy were applied to investigate the binding characteristics of Cd on montmorillonite(Mo...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Du H,Chen W,Cai P,Rong X,Dai K,Peacock CL,Huang Q

    更新日期:2016-01-21 00:00:00

  • Dynamic representation of time in brain states.

    abstract::The ability to process time on the scale of milliseconds and seconds is essential for behaviour. A growing number of studies have started to focus on brain dynamics as a mechanism for temporal encoding. Although there is growing evidence in favour of this view from computational and in vitro studies, there is still a ...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Bueno FD,Morita VC,de Camargo RY,Reyes MB,Caetano MS,Cravo AM

    更新日期:2017-04-10 00:00:00

  • A microfluidic chip for screening individual cancer cells via eavesdropping on autophagy-inducing crosstalk in the stroma niche.

    abstract::Autophagy is a cellular homeostatic mechanism where proteins and organelles are digested and recycled to provide an alternative source of building blocks and energy to cells. The role of autophagy in cancer microenvironment is still poorly understood. Here, we present a microfluidic system allowing monitoring of the c...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Karakas HE,Kim J,Park J,Oh JM,Choi Y,Gozuacik D,Cho YK

    更新日期:2017-05-17 00:00:00

  • Dynamic contact networks of patients and MRSA spread in hospitals.

    abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is a difficult-to-treat infection. Increasing efforts have been taken to mitigate the epidemics and to avoid potential outbreaks in low endemic settings. Understanding the population dynamics of MRSA is essential to identify the causal mechanisms driving the epidemics...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Rocha LEC,Singh V,Esch M,Lenaerts T,Liljeros F,Thorson A

    更新日期:2020-06-09 00:00:00

  • Early-onset obesity dysregulates pulmonary adipocytokine/insulin signaling and induces asthma-like disease in mice.

    abstract::Childhood obesity is a risk factor for asthma, but the molecular mechanisms linking both remain elusive. Since obesity leads to chronic low-grade inflammation and affects metabolic signaling we hypothesized that postnatal hyperalimentation (pHA) induced by maternal high-fat-diet during lactation leads to early-onset o...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Dinger K,Kasper P,Hucklenbruch-Rother E,Vohlen C,Jobst E,Janoschek R,Bae-Gartz I,van Koningsbruggen-Rietschel S,Plank C,Dötsch J,Alejandre Alcázar MA

    更新日期:2016-04-18 00:00:00

  • Physiological demands of a swimming-based video game: Influence of gender, swimming background, and exergame experience.

    abstract::Active video games (exergames) may provide short-term increase in energy expenditure. We explored the effects of gender and prior experience on aerobic and anaerobic energy systems contributions, and the activity profiles of 40 participants playing with a swimming exergame. We recorded oxygen consumption and assessed ...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Soltani P,Figueiredo P,Ribeiro J,Fernandes RJ,Vilas-Boas JP

    更新日期:2017-07-12 00:00:00

  • Simple and compact optode for real-time in-situ temperature detection in very small samples.

    abstract::Real-time in-situ temperature detection is essential in many applications. In this paper, a simple and robust optode, which uses Ruthenium (II) complex as a temperature indicator, has been developed for rapid and sensitive temperature detection in small volume samples (<5 μL). Transmission of excitation light and coll...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Long F,Shi H

    更新日期:2014-05-30 00:00:00

  • Preserved antiviral adaptive immunity following polyclonal antibody immunotherapy for severe murine influenza infection.

    abstract::Passive immunotherapy may have particular benefits for the treatment of severe influenza infection in at-risk populations, however little is known of the impact of passive immunotherapy on the formation of memory responses to the virus. Ideally, passive immunotherapy should attenuate the severity of infection while st...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Stevens NE,Hatjopolous A,Fraser CK,Alsharifi M,Diener KR,Hayball JD

    更新日期:2016-07-06 00:00:00

  • Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells.

    abstract::Resolving the immunogenicity of cells derived from induced pluripotent stem cells (iPSCs) remains an important challenge for cell transplant strategies that use banked allogeneic cells. Thus, we evaluated the immunogenicity of mouse fetal neural stem/progenitor cells (fetus-NSPCs) and iPSC-derived neural stem/progenit...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Itakura G,Ozaki M,Nagoshi N,Kawabata S,Nishiyama Y,Sugai K,Iida T,Kashiwagi R,Ookubo T,Yastake K,Matsubayashi K,Kohyama J,Iwanami A,Matsumoto M,Nakamura M,Okano H

    更新日期:2017-10-11 00:00:00