Abstract:
:Cell-penetrating peptides (CPPs) are efficient vehicles to transport bioactive molecules into the cells. Despite numerous studies the exact mechanism by which CPPs facilitate delivery of cargo to its intracellular target is still debated. The current work presents methods that can be used for tracking CPP/pDNA complexes through endosomal transport and show the role of endosomal transport in the delivery of cargo. Separation of endosomal vesicles by differential centrifugation enables to pinpoint the localization of delivered cargo without labeling it and gives important quantitative information about pDNA trafficing in certain endosomal compartments. Single particle tracking (SPT) allows following individual CPP/cargo complex through endosomal path in live cells, using fluoresently labled cargo and green fluoresent protein expressing cells. These two different methods show similar results about tested NickFect/pDNA complexes intracellular trafficing. NF51 facilitates rapid internalization of complexes into the cells, prolongs their stay in early endosomes and promotes release to cytosol. NF1 is less capable to induce endosomal release and higher amount of complexes are routed to lysosomes for degradation. Our findings offer potential delivery vector for in vivo applications, NF51, where endosomal entrapment has been allayed. Furthermore, these methods are valuable tools to study other CPP-based delivery systems.
journal_name
J Drug Targetjournal_title
Journal of drug targetingauthors
Pärnaste L,Arukuusk P,Zagato E,Braeckmans K,Langel Üdoi
10.3109/1061186X.2015.1095194subject
Has Abstractpub_date
2016-01-01 00:00:00pages
508-19issue
6eissn
1061-186Xissn
1029-2330journal_volume
24pub_type
杂志文章abstract::Cancer is one of the most common diseases all over the world; many people suffer from diverse types of cancer. However, currently there is no exact cure or therapy developed for cancer. On the other hand, nanoparticles are defined as microscopic particles that have dimensions less than 100 nm and they are known for th...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
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journal_title:Journal of drug targeting
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doi:10.3109/10611869308996076
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journal_title:Journal of drug targeting
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doi:10.3109/1061186X.2012.712128
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abstract::The aim of this study was to evaluate the pharmacokinetics (PK), tissue distribution, and the specific drug targeting of cefpiramide sodium-loaded liposomes (CPMS-Lips) compared with cefpiramide sodium solution (CPMS-Sol) in mice. CPMS-Lips were prepared by reverse phase evaporation method. In the PK and biodistributi...
journal_title:Journal of drug targeting
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journal_title:Journal of drug targeting
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journal_title:Journal of drug targeting
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journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2013.840639
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journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
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journal_title:Journal of drug targeting
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journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2016.1144059
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journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2010.492521
更新日期:2011-04-01 00:00:00
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journal_title:Journal of drug targeting
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doi:10.3109/10611869909085521
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journal_title:Journal of drug targeting
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journal_title:Journal of drug targeting
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doi:10.3109/10611869408996811
更新日期:1994-01-01 00:00:00
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journal_title:Journal of drug targeting
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doi:10.1080/10611860410001724468
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journal_title:Journal of drug targeting
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doi:10.3109/1061186X.2014.916712
更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:High expression of glucagon-like peptide-1 receptor (GLP-1R) in insulinoma supplies a potential drug target for tumor imaging. Exendin-4 can specifically bind to GLP-1R as an agonist and its analogs are extensively used in receptor imaging studies. PURPOSE:A new GLP-1R imaging agent, [(18)F]AlF-NOTA-MAL-Cys...
journal_title:Journal of drug targeting
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doi:10.3109/1061186X.2015.1020808
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journal_title:Journal of drug targeting
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journal_title:Journal of drug targeting
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journal_title:Journal of drug targeting
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journal_title:Journal of drug targeting
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journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869808995875
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journal_title:Journal of drug targeting
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doi:10.3109/10611869909085514
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journal_title:Journal of drug targeting
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doi:10.3109/10611869509059216
更新日期:1995-01-01 00:00:00
abstract::Chemotherapy is still a major challenge for in vivo drug targeting to macrophages. Toxicity remains the major obstacle for the most potent drugs already known in the therapy of leishmaniasis. Thus, new drugs and new delivery systems are sought. By using different vesicular delivery modes e.g. liposomes, niosomes, micr...
journal_title:Journal of drug targeting
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journal_title:Journal of drug targeting
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更新日期:2002-03-01 00:00:00
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journal_title:Journal of drug targeting
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doi:10.3109/10611869308996086
更新日期:1993-01-01 00:00:00
abstract::Targeting of anti-tumor drugs to the urinary bladder for the treatment of bladder carcinoma may be useful, since these agents generally have a low degree of urinary excretion and are highly toxic elsewhere in the body. The anti-tumor drug doxorubicin was coupled to the low-molecular weight protein lysozyme via the aci...
journal_title:Journal of drug targeting
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doi:10.1080/10611860290007568
更新日期:2002-02-01 00:00:00
abstract::Nanoparticles form the fundamental building blocks for many exciting applications in various scientific disciplines due to its unique features such as large surface to mass ratio, targeting potential, ability to adsorbed and carry other compound which makes them suitable for biomedical applications. However, the probl...
journal_title:Journal of drug targeting
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