Abstract:
:We report the discovery and confirmation of 23 novel mutations with previously undocumented role in isoniazid (INH) drug resistance, in catalase-peroxidase (katG) gene of Mycobacterium tuberculosis (Mtb) isolates. With these mutations, a synonymous mutation in fabG1 (g609a), and two canonical mutations, we were able to explain 98% of the phenotypic resistance observed in 366 clinical Mtb isolates collected from four high tuberculosis (TB)-burden countries: India, Moldova, Philippines, and South Africa. We conducted overlapping targeted and whole-genome sequencing for variant discovery in all clinical isolates with a variety of INH-resistant phenotypes. Our analysis showed that just two canonical mutations (katG 315AGC-ACC and inhA promoter-15C-T) identified 89.5% of resistance phenotypes in our collection. Inclusion of the 23 novel mutations reported here, and the previously documented point mutation in fabG1, increased the sensitivity of these mutations as markers of INH resistance to 98%. Only six (2%) of the 332 resistant isolates in our collection did not harbor one or more of these mutations. The third most prevalent substitution, at inhA promoter position -8, present in 39 resistant isolates, was of no diagnostic significance since it always co-occurred with katG 315. 79% of our isolates harboring novel mutations belong to genetic group 1 indicating a higher tendency for this group to go down an uncommon evolutionary path and evade molecular diagnostics. The results of this study contribute to our understanding of the mechanisms of INH resistance in Mtb isolates that lack the canonical mutations and could improve the sensitivity of next generation molecular diagnostics.
journal_name
Emerg Microbes Infectjournal_title
Emerging microbes & infectionsauthors
Torres JN,Paul LV,Rodwell TC,Victor TC,Amallraja AM,Elghraoui A,Goodmanson AP,Ramirez-Busby SM,Chawla A,Zadorozhny V,Streicher EM,Sirgel FA,Catanzaro D,Rodrigues C,Gler MT,Crudu V,Catanzaro A,Valafar Fdoi
10.1038/emi.2015.42subject
Has Abstractpub_date
2015-07-01 00:00:00pages
e42issue
7issn
2222-1751journal_volume
4pub_type
杂志文章abstract::The isolation and characterization of monoclonal broadly neutralizing antibodies (nAbs) from natural HIV-1-infected individuals play very important roles in understanding nAb responses to HIV-1 infection and designing vaccines and therapeutics. Many broadly nAbs have been isolated from individuals infected with HIV-1 ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/s41426-018-0175-1
更新日期:2018-11-01 00:00:00
abstract::Three leading infectious disease experts in China were invited to share their bedside observations in the management of COVID-19 patients. Professor Taisheng Li was sent to Wuhan to provide frontline medical care. He depicts the clinical course of SARS-CoV-2 infection. Furthermore, he observes the significant abnormal...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2020.1741327
更新日期:2020-12-01 00:00:00
abstract::The recently emerged novel coronavirus, "severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)", caused a highly contagious disease called coronavirus disease 2019 (COVID-19). The virus was first reported from Wuhan city in China in December, 2019, which in less than three months spread throughout the globe and...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
doi:10.1080/22221751.2020.1775132
更新日期:2020-12-01 00:00:00
abstract::We conducted this study to describe the dynamics of the acquisition of respiratory pathogens, their potential interactions and risk factors for possible lower respiratory tract infection symptoms (LRTI) among French pilgrims during the 2018 Hajj. Each participant underwent four successive systematic nasopharyngeal swa...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1693247
更新日期:2019-01-01 00:00:00
abstract::Norovirus (NoV) is now recognized as a leading cause of nonbacterial acute gastroenteritis; however, the NoV GII.17 genotype has rarely been reported as the predominant genotype in clinical diarrhea cases. During the winter of 2014–2015, the GII.17 genotype, together with the NoV GII.4 genotype, dominated in sporadic ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/emi.2015.67
更新日期:2015-11-01 00:00:00
abstract::The Beijing genotype comprises a highly disseminated strain type that is frequently associated with multidrug resistant (MDR) tuberculosis (TB) and increased transmissibility but, countries such as Portugal and Guinea-Bissau fall outside the regions phylogeographically associated with this specific genotype. Neverthel...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2020.1774425
更新日期:2020-12-01 00:00:00
abstract::The continuing pandemic threat posed by avian influenza A/H5N1 viruses calls for improved insights into their evolution during human infection. We performed whole genome deep sequencing of respiratory specimens from 44 H5N1-infected individuals from Indonesia and found substantial within-host viral diversity. At nearl...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1575700
更新日期:2019-01-01 00:00:00
abstract::Saffold virus (SAFV), a newly discovered human cardiovirus of the Picornaviridae family, causes widespread infection among children, as shown by previous seroprevalence studies. To determine the host cell range of SAFV and its cytopathogenicity, eight mammalian cell lines that were available in the laboratory were scr...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/emi.2014.15
更新日期:2014-02-01 00:00:00
abstract::West Nile virus (WNV) is an arthropod-borne flavivirus of high medical and veterinary importance. The main vectors for WNV are mosquito species of the Culex genus that transmit WNV among birds, and occasionally to humans and horses, which are 'dead-end' hosts. Recently, several studies have been published that aimed t...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
doi:10.1038/emi.2017.82
更新日期:2017-11-08 00:00:00
abstract::Highly pathogenic avian influenza H5N1 viruses were first isolated in Bangladesh in February 2007. Subsequently, clades 2.2.2, 2.3.4.2 and 2.3.2.1a were identified in Bangladesh, and our previous surveillance data revealed that by the end of 2014, the circulating viruses exclusively comprised clade 2.3.2.1a. We recent...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/emi.2017.60
更新日期:2017-08-09 00:00:00
abstract::Previous studies have shown that livestock (LA)-MRSA ST398 evolved from a human-adapted methicillin-susceptible S. aureus (MSSA) clone. However, detailed information regarding ST9 is still unclear. Here, we characterized a community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) ST9-SCCmec XII isolat...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2020.1848354
更新日期:2020-12-01 00:00:00
abstract::Emerging studies have indicated that some penaeidins restrict virus infection; however, the mechanism(s) involved are poorly understood. In the present study, we uncovered that penaeidins are a novel family of antiviral effectors against white spot syndrome virus (WSSV), which antagonize the envelope proteins to block...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2020.1729068
更新日期:2020-12-01 00:00:00
abstract::Interleukin 17 (IL-17) and its closest relative, IL-17F, have recently drawn much attention in the field of immunology. IL-17 and IL-17F are expressed by a distinct type of T cells, T helper 17 cells and certain other lymphocytes. These cytokines play key regulatory roles in host defense and inflammatory diseases. In ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
doi:10.1038/emi.2013.58
更新日期:2013-09-01 00:00:00
abstract::The three unprecedented outbreaks of emerging human coronavirus (HCoV) infections at the beginning of the twenty-first century have highlighted the necessity for readily available, accurate and fast diagnostic testing methods. The laboratory diagnostic methods for human coronavirus infections have evolved substantiall...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
doi:10.1080/22221751.2020.1745095
更新日期:2020-12-01 00:00:00
abstract::Accumulating evidence indicates that bacterial metabolism plays an important role in virulence. Acetyl phosphate (AcP), the high-energy intermediate of the phosphotransacetylase-acetate kinase pathway, is the major acetyl donor in E. coli. PhoP is an essential transcription factor for bacterial virulence. Here, we sho...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2018.1558963
更新日期:2019-01-01 00:00:00
abstract::We report the first case of severe pneumonia due to co-infection with the emerging avian influenza A (H5N1) virus subclade 2.3.2.1 and Mycoplasma pneumoniae. The patient was a returning traveller who had visited a poultry market in South China. We then review the epidemiology, virology, interspecies barrier limiting p...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
doi:10.1038/emi.2012.24
更新日期:2012-09-01 00:00:00
abstract::Zika virus (ZIKV) infection during pregnancy is associated with congenital brain abnormalities, a finding that highlights the urgent need to understand mother-to-fetus transmission mechanisms. Human umbilical cord mesenchymal stem cells (hUCMSCs) are susceptible to ZIKV infection but the underlying mechanisms of viral...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2020.1821581
更新日期:2020-12-01 00:00:00
abstract::Candida albicans is a major human fungal pathogen, capable of switching among a range of morphological types, such as the yeast form, including white and opaque cell types and the GUT (gastrointestinally induced transition) cell type, the filamentous form, including hyphal and pseudohyphal cell types, and chlamydospor...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2020.1729067
更新日期:2020-02-21 00:00:00
abstract::Background: The COVID-19 pandemic represents an unprecedented global challenge and implicates a wide range of burden on medical professionals. Here, we evaluated the perception of the COVID-19 pandemic among medical professionals in Germany. Methods: A total of n = 2827 medical professionals participated in an online ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2020.1785951
更新日期:2020-12-01 00:00:00
abstract::Macrolides such as erythromycin are the empirical treatment of Bordetella pertussis infections. China has experienced an increase in erythromycin-resistant B. pertussis isolates since they were first reported in 2013. Here, we undertook a genomic study on Chinese B. pertussis isolates from 2012 to 2015 to elucidate th...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1587315
更新日期:2019-01-01 00:00:00
abstract::Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with droplets and contact as the main means of transmission. Since the first case appeared in Wuhan, China, in December 2019, the outbreak has gradually spread nationwide. Up to now, acco...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2020.1746199
更新日期:2020-12-01 00:00:00
abstract::Monoclonal antibodies (mAbs), which currently constitute the main class of biotherapeutics, are now recognized as major medical tools that are increasingly being considered to fight severe viral infections. Indeed, the number of antiviral mAbs developed in recent years has grown exponentially. Although their direct ef...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
doi:10.1038/emi.2016.97
更新日期:2016-08-17 00:00:00
abstract::Occult hepatitis B virus infection (OBI) is a low-level asymptomatic phase of HBV infection. Evidence of OBI clinical relevance is emerging but the mechanisms of its occurrence remain unclear. In this study, the molecular characteristics of 97 confirmed OBI from Chinese blood donors were analyzed and relevant mutation...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1663130
更新日期:2019-01-01 00:00:00
abstract::The progressive anthropization of caves for food resources or economic purposes increases human exposure to pathogens that naturally infect cave-dwelling animals. The presence of wild or domestic animals in the immediate surroundings of caves also may contribute to increasing the risk of emergence of such pathogens. S...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
doi:10.1038/emi.2017.6
更新日期:2017-04-12 00:00:00
abstract::A novel equine parvovirus, equine parvovirus-hepatitis (EqPV-H), was first discovered in a horse that died of equine serum hepatitis in the USA in 2018. EqPV-H was shown to be a novel etiological agent associated with equine serum hepatitis. Following this initial report, no additional studies on EqPV-H have been publ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/s41426-018-0174-2
更新日期:2018-10-23 00:00:00
abstract::Serology detection is recognized for its sensitivity in convalescent patients with COVID-19, in comparison with nucleic acid amplification tests (NAATs). This article aimed to evaluate the diagnostic accuracy of serologic methods for COVID-19 based on assay design and post-symptom-onset intervals. Two authors independ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,meta分析
doi:10.1080/22221751.2020.1826362
更新日期:2020-12-01 00:00:00
abstract::The highly pathogenic avian influenza A (H5N6) virus has caused sporadic human infections with a high case fatality rate. Due to the continuous evolution of this virus subtype and its ability to transmit to humans, there is an urgent need to develop effective antiviral therapeutics. In this study, a murine monoclonal ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1708215
更新日期:2020-01-06 00:00:00
abstract::COVID-19, the worst pandemic in 100 years, has rapidly spread to the entire world in 2 months since its early report in January 2020. Based on the publicly available data sources, we developed a simple mathematic modeling approach to track the outbreaks of COVID-19 in the US and three selected states: New York, Michig...
journal_title:Emerging microbes & infections
pub_type: 信件
doi:10.1080/22221751.2020.1760146
更新日期:2020-12-01 00:00:00
abstract::Acinetobacter baumannii is a notorious pathogen that has emerged as a healthcare nightmare in recent years because it causes serious infections that are associated with high morbidity and mortality rates. Due to its exceptional ability to acquire resistance to almost all available antibiotics, A. baumannii is currentl...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
doi:10.1038/s41426-018-0030-4
更新日期:2018-03-21 00:00:00
abstract::Lentiviruses harbour high genetic variability for efficient evasion from host immunity. An attenuated equine infectious anaemia (EIA) vaccine was developed decades ago in China and presented remarkably robust protection against EIA. The vaccine was recently proven to have high genomic diversity, particular in env. How...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2020.1773323
更新日期:2020-12-01 00:00:00