Non-linear optical flow cytometry using a scanned, Bessel beam light-sheet.

Abstract:

:Modern flow cytometry instruments have become vital tools for high-throughput analysis of single cells. However, as issues with the cellular labeling techniques often used in flow cytometry have become more of a concern, the development of label-free modalities for cellular analysis is increasingly desired. Non-linear optical phenomena (NLO) are of growing interest for label-free analysis because of the ability to measure the intrinsic optical response of biomolecules found in cells. We demonstrate that a light-sheet consisting of a scanned Bessel beam is an optimal excitation geometry for efficiently generating NLO signals in a microfluidic environment. The balance of photon density and cross-sectional area provided by the light-sheet allowed significantly larger two-photon fluorescence intensities to be measured in a model polystyrene microparticle system compared to measurements made using other excitation focal geometries, including a relaxed Gaussian excitation beam often used in conventional flow cytometers.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Collier BB,Awasthi S,Lieu DK,Chan JW

doi

10.1038/srep10751

subject

Has Abstract

pub_date

2015-05-29 00:00:00

pages

10751

issn

2045-2322

pii

srep10751

journal_volume

5

pub_type

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