当前位置： SCI文献检索 > JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS期刊下所有文献 > A new model of detrusor overactivity in conscious rats induced by retinyl acetate instillation.

A new model of detrusor overactivity in conscious rats induced by retinyl acetate instillation.

Abstract：

INTRODUCTION:A credible animal overactive bladder model used in basic research is an indispensable harbinger of safe and ethical clinical trials on human subjects. Our objective was to develop a new animal model of a hyperactive bladder that will be void of inflammatory urothelium lesions and display significant sensitivity to muscarinic receptor antagonists. METHODS:To examine the influence of 0.75% retinyl acetate solution on cystometric parameters, it was infused into the bladder for 5min. Cystometric studies with physiological saline were performed in conscious unrestrained rats 3days later. To examine the influence of retinyl acetate, acetic acid or cyclophosphamide on morphology of urinary bladders, the bladders were subjected to histopathological examination. RESULTS:We demonstrated that in rats subject to previous 5-minute bladder instillations with retinyl acetate, an increase of basal pressure, threshold pressure, micturition voiding pressure, bladder contraction duration, relaxation time, detrusor overactivity index, nonvoiding contraction frequency and amplitude occurs. On the other hand, a decrease in voided volume, post-void residual, volume threshold, voiding efficiency, intercontraction interval, bladder compliance and volume threshold to elicit nonvoiding contractions was observed. Administration of oxybutynin chloride (0.5mg/kg, i.v.) reversed changes of cystometric parameters evoked by retinyl acetate. Contrary to acetic acid and cyclophosphamide, bladders subjected to retinyl acetate infusion had no signs of bladder inflammation. DISCUSSION:The results obtained indicate that transient infusion of 0.75% retinyl acetate can induce detrusor overactivity, which is often observed in patients with overactive bladder syndrome (OAB). In addition, it was demonstrated that stimulating afferent C-fibres using retinyl acetate did not induce evident histopathological inflammatory lesions in the urinary bladder wall. It appears that in the future this model can prove useful in gaining more knowledge on the pathophysiology of OAB, and contribute to the preparation of new, more effective options of OAB pharmacotherapy.

journal_name

J Pharmacol Toxicol Methods

journal_title

Journal of pharmacological and toxicological methods

authors

Wróbel A,Łańcut M,Rechberger T

doi

10.1016/j.vascn.2015.04.006

subject

Has Abstract

pub_date

2015-07-01 00:00:00

pages

7-16

eissn

1056-8719

issn

1873-488X

pii

S1056-8719(15)00044-1

journal_volume

74

pub_type

杂志文章

相关文献

JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS文献大全
  • Determination of nitric oxide generation in mammalian neurons using dichlorofluorescin diacetate and flow cytometry.

    abstract：:A method for the rapid detection of intracellular nitric oxide (NO) generation in dissociated cerebellar granule cells using dichlorofluorescin (DCFH) and flow cytometry was developed. DCFH can be oxidized specifically by NO and this was assessed by 1) the use of SIN-1 (10 nM-100 microM), an NO donor, that induced a c...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/s1056-8719(97)00066-x

    authors： Gabriel C,Camins A,Sureda FX,Aquirre L,Escubedo E,Pallàs M,Camarasa J

    更新日期：1997-10-01 00:00:00

  • A simple, automatic method for morphometric analysis of the left ventricle in rats with myocardial infarction.

    abstract：:Induction of acute myocardial infarction in the rat is an established model for studying effects of therapeutic interventions. Images of sections of the rat left ventricle, stained with nitroblue tetrazolium, were digitized and several parameters estimated by dedicated software on an image analyzer (IBAS 2.0). The met...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/1056-8719(95)00019-e

    authors： Porzio S,Masseroli M,Messori A,Forloni G,Olivetti G,Jeremic G,Riva E,Luvarà G,Latini R

    更新日期：1995-08-01 00:00:00

  • Progress in computational toxicology.

    abstract：INTRODUCTION:Computational methods have been widely applied to toxicology across pharmaceutical, consumer product and environmental fields over the past decade. Progress in computational toxicology is now reviewed. METHODS:A literature review was performed on computational models for hepatotoxicity (e.g. for drug-indu...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章,评审

    doi：10.1016/j.vascn.2013.12.003

    authors： Ekins S

    更新日期：2014-03-01 00:00:00

  • A novel predictive pharmacokinetic/pharmacodynamic model of repolarization prolongation derived from the effects of terfenadine, cisapride and E-4031 in the conscious chronic av node--ablated, His bundle-paced dog.

    abstract：INTRODUCTION:Terfenadine, cisapride, and E-4031, three drugs that prolong ventricular repolarization, were selected to evaluate the sensitivity of the conscious chronic atrioventricular node--ablated, His bundle-paced Dog for defining drug induced cardiac repolarization prolongation. A novel predictive pharmacokinetic/...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2005.02.003

    authors： Nolan ER,Feng MR,Koup JR,Liu J,Turluck D,Zhang Y,Paulissen JB,Olivier NB,Miller T,Bailie MB

    更新日期：2006-01-01 00:00:00

  • Rhesus monkey model for concurrent analyses of in vivo selectivity, pharmacokinetics and pharmacodynamics of aldosterone synthase inhibitors.

    abstract：INTRODUCTION:In vivo profiles of aldosterone synthase inhibitors (ASIs) have been investigated utilizing various rodent models. Due to lack of CYP17 activity, rodents produce corticosterone rather than cortisol as that of humans, which raised concern to their effectiveness in translational pharmacological characterizat...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2014.09.011

    authors： Cai TQ,Stribling S,Tong X,Xu L,Wisniewski T,Fontenot JA,Struthers M,Akinsanya KO

    更新日期：2015-01-01 00:00:00

  • Radioimmunoassay of substance P in lung perfusate.

    abstract：:A radioimmunoassay of the undecapeptide substance P (SP) and its application in rabbit lung superfusate has been developed. The assay was based on the use of a radioactive tracer, 125I-SP-Tyr8, which showed, with an excess of antibodies, a specific binding greater than 90% and a nonspecific binding lower than 2%. This...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/s1056-8719(98)00012-4

    authors： Nemmar A,Gustin P,Delaunois A,Beckers JF,Sulon J

    更新日期：1998-03-01 00:00:00

  • Critical care of sub-lethal irradiated transgenic mice using a complete soft food formula-DietGel76A™.

    abstract：:The objective of this research is to determine whether the administration of a complete soft food formula to sub-lethal irradiated animals from three different transgenic mouse strains over a period of 21 consecutive days will have a significant impact on the clinical signs, and the general survival rate of the animal...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2014.12.003

    authors： Jumanca OI,Palmer J

    更新日期：2015-01-01 00:00:00

  • Origins, practices and future of safety pharmacology.

    abstract：:The origins of safety pharmacology are grounded upon observations that organ functions (like organ structures) can be toxicological targets in humans exposed to novel therapeutic agents, and that drug effects on organ functions (unlike organ structures) are not readily detected by standard toxicological testing. Safet...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章,评审

    doi：10.1016/j.vascn.2004.02.007

    authors： Bass A,Kinter L,Williams P

    更新日期：2004-05-01 00:00:00

  • ECG arrhythmias in non-implanted vs. telemetry-implanted dogs: need for screening before and sufficient recovery time after implantation.

    abstract：INTRODUCTION:The purpose of this study was: (1) to characterize and assess the incidence of spontaneous arrhythmias in totally naive Beagle dogs (n=51; 10 males+41 females): (2) to study the effects of permanent ECG- and LVP-probe telemetry implants both acutely and up to 233days after surgery in a subset of 11 female ...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2011.04.001

    authors： Cools F,Janssens S,Vanlommel A,Teisman A,Towart R,Gallacher DJ

    更新日期：2011-07-01 00:00:00

  • The PEG-switch assay: a fast semi-quantitative method to determine protein reversible cysteine oxidation.

    abstract：INTRODUCTION:Reversible protein cysteine oxidation is recognised as a pivotal post-transitional modification that transduces physiological as well as pathological signalling. Pharmacological interventions that target specific sources of oxidant formation are currently being trialled to ascertain their potential ability...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2013.07.001

    authors： Burgoyne JR,Oviosu O,Eaton P

    更新日期：2013-11-01 00:00:00

  • A comparison of rectal and subcutaneous body temperature measurement in the common marmoset.

    abstract：:Two methods of measuring body temperature were compared in common marmosets. Subcutaneous temperatures were measured remotely via previously implanted subcutaneous microchips (Plexx BV, IPTT-100) prior to measurement of rectal temperature using a conventional rectal probe. Marmosets were treated with saline or the bra...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/s1056-8719(98)00030-6

    authors： Cilia J,Piper DC,Upton N,Hagan JJ

    更新日期：1998-07-01 00:00:00

  • An animal model for the assessment of gingival lesions.

    abstract：:A model of gingival inflammation was performed in Sprague-Dawley rats weighing 180-200 g. Mechanical bamboo stick-induced injury was inflammatory when bacteria contaminated the sticks. Bacteria were first obtained from gingival fluid collected from a patient with adult periodontitis. Another strain from Institut Paste...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/1056-8719(94)90070-1

    authors： Guessous F,Huynh C,N'Guyen H,Godeau G,Giroud JP,Meyer J,Hornebeck W,Roch-Arveiller M

    更新日期：1994-11-01 00:00:00

  • Prevention of ischemia-induced cerebral hypothermia by controlling the environmental temperature.

    abstract：:Regulation of brain temperature during and after cerebral ischemia plays an important role in the evolution of neuronal damage. Therefore, it is essential to maintain brain temperature at 37 degrees C during and after the ischemic insult. To get information about brain temperature during the experimental procedure, re...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/1056-8719(92)90016-t

    authors： Seif el Nasr M,Nuglisch J,Krieglstein J

    更新日期：1992-03-01 00:00:00

  • Transducer abstraction: a novel approach to the detection of partial agonist efficacy in radioligand binding studies.

    abstract：:The properties of the ternary complex model (TCM) of drug action at G protein-coupled receptors (GPCRs) were examined, using theoretical computer simulations, with regard to the predicted effects of the presence of a fixed concentration of one agonist on the competition binding profile of another. Subsequently, the bi...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/s1056-8719(00)00078-2

    authors： Christopoulos A,Grant MK,El-Fakahany EE

    更新日期：2000-01-01 00:00:00

  • CSAHi study: Evaluation of multi-electrode array in combination with human iPS cell-derived cardiomyocytes to predict drug-induced QT prolongation and arrhythmia--effects of 7 reference compounds at 10 facilities.

    abstract：INTRODUCTION:Drug-induced QT prolongation is a major safety issue during drug development because it may lead to lethal ventricular arrhythmias. In this study, we evaluated the utility of multi-electrode arrays (MEA) with human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) to predict drug-induced QT p...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.vascn.2015.12.002

    authors： Kitaguchi T,Moriyama Y,Taniguchi T,Ojima A,Ando H,Uda T,Otabe K,Oguchi M,Shimizu S,Saito H,Morita M,Toratani A,Asayama M,Yamamoto W,Matsumoto E,Saji D,Ohnaka H,Tanaka K,Washio I,Miyamoto N

    更新日期：2016-03-01 00:00:00

  • Characterization of immune cell, endothelial, and renal responses upon experimental human endotoxemia.

    abstract：INTRODUCTION:Although the effects of relatively high concentrations of endotoxin on endothelial activation/dysfunction and kidney markers has been described in literature, detailed insight in the LPS concentration-effect relationship, the magnitude, variability and timing of the response, and potential effects of endot...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vascn.2017.10.004

    authors： van Poelgeest EP,Dillingh MR,de Kam M,Malone KE,Kemper M,Stroes ESG,Burggraaf J,Moerland M

    更新日期：2018-01-01 00:00:00

  • Down for the count: The critical endpoint in ototoxicity remains the cytocochleogram.

    abstract：:There are three major assays that must be conducted in standard investigational new drug (IND) -enabling ototoxicity study designs: 1) functional acoustic threshold measurements (Auditory Brainstem Respsonse, ABR); 2) otohistopathology and 3) cytocochleograms. We provide evidence to demonstrate the unreliability of au...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章,评审

    doi：10.1016/j.vascn.2017.09.254

    authors： Gauvin DV,Yoder J,Koch A,Zimmermann ZJ,Tapp RL

    更新日期：2017-01-01 00:00:00

  • A capillary electrophoresis based approach for the identification of anti-drug antibodies against camelid VHH biologics (Nanobodies®).

    abstract：:Undesired immune responses against protein therapeutics may adversely affect the pharmacokinetics, efficacy, and safety of the product. The presence of anti-drug-antibodies (ADA) has been the key determinant of immunogenic responses. Here we describe the use of a capillary electrophoresis platform for the identificati...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2020.106872

    authors： Wiswell D,Neupane D,Chen M,Bowman EP,Linn D,Sawant A,Chackerian A,Zhang S,Escandón E

    更新日期：2020-01-01 00:00:00

  • An integrated system for video and telemetric electroencephalographic recording to measure behavioural and physiological parameters.

    abstract：INTRODUCTION:The combined evaluation of physiology and behaviour allows a complete and more comprehensive pre-clinical assessment of central nervous system (CNS) function. An integrated video-telemetric electroencephalography (Video-tEEG) system, which allows the simultaneous and continuous recording of EEG and video i...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2010.04.004

    authors： Moscardo E,Rostello C

    更新日期：2010-07-01 00:00:00

  • Automated enzyme inhibition assay method for the determination of atorvastatin-derived HMG-CoA reductase inhibitors in human plasma using radioactivity detection.

    abstract：INTRODUCTION:A Tecan-based enzyme inhibition assay has been developed for the determination of atorvastatin-derived 'active' and 'total' (active inhibitors plus atorvastatin lactone and other potential inhibitors following base hydrolysis) 3-hydroxy-3-methylglutaryl-Coenzyme A (HMG-CoA) reductase inhibitor concentratio...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2007.06.002

    authors： Valesky RJ,Liu L,Musson DG,Zhao JJ

    更新日期：2008-01-01 00:00:00

  • High-content imaging analysis for detecting the loss of drebrin clusters along dendrites in cultured hippocampal neurons.

    abstract：INTRODUCTION:Detection of drug effects on neuronal synapses is important for predicting their adverse effects. We have used drebrin as a marker to detect the synaptic changes in cultured neurons. High concentration of glutamate decreases the amount of drebrin in synapses. To increase the availability of this method for...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2019.106607

    authors： Hanamura K,Koganezawa N,Kamiyama K,Tanaka N,Oka T,Yamamura M,Sekino Y,Shirao T

    更新日期：2019-01-01 00:00:00

  • Cholemic transgenic mice: a novel animal model to investigate the effects of bile acids.

    abstract：INTRODUCTION:Laboratory investigations into cholestatic liver disease and the effects of cholemia on organ function are long-standing subjects of scientific enquiry. A widely-used strategy to investigate these topics relies on animal-based research using experimental animal models. Targeted inactivation of the spgp gen...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2004.06.002

    authors： Ljubuncic P,Yousef I,Bomzon A

    更新日期：2004-11-01 00:00:00

  • Safety pharmacology methods and models in an evolving regulatory environment.

    abstract：:This editorial prefaces the annual themed issue on safety pharmacology (SP) methods published in the Journal of Pharmacological and Toxicological Methods (JPTM). We highlight here the content derived from the recent 2016 Safety Pharmacology Society (SPS), Canadian Society of Pharmacology and Therapeutics (CSPT), and J...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 社论

    doi：10.1016/j.vascn.2017.04.012

    authors： Pugsley MK,de Korte T,Authier S,Huang H,Accardi MV,Curtis MJ

    更新日期：2017-09-01 00:00:00

  • Physiologic and pharmacologic factors that affect myocardial relaxation.

    abstract：:Evaluation of the myocardial relaxation has become important in the last years. An impaired relaxation may precede contractile dysfunctions and even cause heart failure. To treat this impaired lusitropism it is necessary to properly assess the lusitropic state of the heart and understand how drugs affect the cellular ...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章,评审

    doi：10.1016/1056-8719(94)90011-6

    authors： Vittone L,Mundiña-Weilenmann C,Mattiazzi A,Cingolani H

    更新日期：1994-09-01 00:00:00

  • Validation of a voltage-sensitive dye (di-4-ANEPPS)-based method for assessing drug-induced delayed repolarisation in beagle dog left ventricular midmyocardial myocytes.

    abstract：INTRODUCTION:Evaluation of drug candidates in in-vitro assays of action potential duration (APD) is one component of preclinical safety assessment. Current assays are limited by technically-demanding, time-consuming electrophysiological methods. This study aimed to assess whether a voltage-sensitive dye-based assay cou...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2009.03.005

    authors： Hardy ME,Pollard CE,Small BG,Bridgland-Taylor M,Woods AJ,Valentin JP,Abi-Gerges N

    更新日期：2009-07-01 00:00:00

  • Effect of multiple freeze-thaw cycles of cytoplasm samples on the activity of antioxidant enzymes.

    abstract：INTRODUCTION:In this paper the activity of cytoplasmatic antioxidant enzymes after multiple freeze-thaw cycles of samples of cytoplasm was assayed in order to characterise the limits that would be necessary to impose on experimental design. METHODS:The activities of antioxidant enzymes in samples of rat liver cytoplas...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2005.03.002

    authors： Murias M,Rachtan M,Jodynis-Liebert J

    更新日期：2005-09-01 00:00:00

  • Ventricular cycle length irregularity affects the correlation between ventricular rate and coronary flow in isolated, Langendorff perfused guinea pig hearts.

    abstract：INTRODUCTION:Heart rate affects coronary flow, but the mechanism is complex. The relationship between rhythm and flow is unclear, especially in experimental settings used for determining drug actions. The present study examined whether ventricular irregularity influences coronary flow independently of heart rate. METH...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2015.10.001

    authors： Takács H,Kui P,Farkas AS,Sarusi A,Forster T,Papp JG,Varró A,Curtis MJ,Shattock MJ,Farkas A

    更新日期：2016-01-01 00:00:00

  • The drug discrimination assay: Interpretative value of partial generalization for drug schedule control actions.

    abstract：:The U.S. Food & Drug Administration (FDA) has issued a final guidance document on the preclinical determination of abuse potential that must be conducted in animals for all new molecular entities (NMEs) submitted for a new drug application (NDA). Under statutory restrictions government guidance documents serve only as...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2017.11.005

    authors： Gauvin DV,Zimmermann ZJ,Code R,Baird TJ

    更新日期：2018-01-01 00:00:00

  • Rapid HPLC assay with coulometric detection for norepinephrine and 3-methoxy-4-hydroxyphenylglycol in the mouse brain.

    abstract：:For the rapid assay of norepinephrine (NE) and its major metabolite, 3-methoxy-4-hydroxyphenylglycol (MHPG) in the mouse brain, we developed a simple method using isocratic HPLC with coulometric detection. This method permits NE and MHPG assay within 5 min in one chromatographic run. Within-run coefficients of variati...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/1056-8719(94)90060-4

    authors： Murai S,Saito H,Masuda Y,Ohkubo N,Itoh T

    更新日期：1994-10-01 00:00:00

  • The influence of vancomycin concentration and the pH of plasma on vancomycin protein binding.

    abstract：:A review of numerous studies of the protein binding of vancomycin suggests major discrepancies among their results. The reported percent protein binding of vancomycin varies from 0% to 98%. The influence of pH and concentration on the protein binding of vancomycin was investigated in this study. There was a significan...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/1056-8719(92)90066-a

    authors： Chen Y,Norris RL,Schneider JJ,Ravenscroft PJ

    更新日期：1992-08-01 00:00:00