Abstract:
:Hybridization analysis of mRNA with a cDNA probe for human sucrase-isomaltase, pulse-chase experiments with L-[35S]-methionine followed by SDS-PAGE, and immunofluorescence detection of sucrase-isomaltase were used to analyze the level(s) at which forskolin interferes with the expression of the enzyme in Caco-2 cells in culture. Three effects are observed in Caco-2 cells treated with forskolin: 1) a marked decrease in the level of sucrase-isomaltase mRNA, 2) a marked decrease in the biosynthesis of the enzyme without any alteration of its stability, and 3) an almost total inhibition of its transport to the brush border membrane. All three effects are reversible when the drug is removed from the culture medium, though this reversibility is asynchronous: transport to the brush border membrane resumes after 24 h, sucrase-isomaltase mRNA levels are back to the normal after 5 days, whereas the biosynthesis of the enzyme, although increasing progressively, remains lower than in control cells, even 10 days after removal of the drug. The possibility that some effects are directly dependent on cAMP and others a consequence of changes in glucose metabolism is discussed.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Rousset M,Chantret I,Darmoul D,Trugnan G,Sapin C,Green F,Swallow D,Zweibaum Adoi
10.1002/jcp.1041410322subject
Has Abstractpub_date
1989-12-01 00:00:00pages
627-35issue
3eissn
0021-9541issn
1097-4652journal_volume
141pub_type
杂志文章abstract::Indian hedgehog (Ihh) is produced by growth plate pre-hypertrophic chondrocytes, and is an important regulator of endochondral ossification. However, little is known about the regulation of Ihh in chondrocytes. We have examined the role of integrins and mitogen-activated protein (MAP) kinases in Ihh mRNA regulation in...
journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:1992-09-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of cellular physiology
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pub_type: 杂志文章,评审
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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abstract::The coupled action of the Na(+)/H(+) exchanger NHE1 and the HCO3(-)/Cl(-) exchanger AE2 constitutes the principal mechanism for acute correction of decreased cell volume in mammalian somatic cells, while, when acting separately, they regulate intracellular pH. It was previously found that AE2 becomes inactivated durin...
journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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更新日期:2006-11-01 00:00:00
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更新日期:2019-11-01 00:00:00
abstract::Tumor necrosis factor-α (TNF-α) is a pluripotent signaling molecule. The biological effect of TNF-α includes slowing down osteogenic differentiation, which can lead to bone dysplasia in long-term inflammatory microenvironments. Signal transducer and activator of transcription 3 (STAT3)-interacting protein 1 (StIP1, al...
journal_title:Journal of cellular physiology
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更新日期:2019-08-01 00:00:00