Abstract：
:Cyclic stretch is an artificial model of mechanical force loading, which induces the reorientation of vascular endothelial cells and their stress fibers in a direction perpendicular to the stretch axis. Rho family GTPases are crucial for cyclic-stretch-induced endothelial cell reorientation; however, the mechanism underlying stretch-induced activation of Rho family GTPases is unknown. A screen of short hairpin RNAs targeting 63 Rho guanine nucleotide exchange factors (Rho-GEFs) revealed that at least 11 Rho-GEFs – Abr, alsin, ARHGEF10, Bcr, GEF-H1 (also known as ARHGEF2), LARG (also known as ARHGEF12), p190RhoGEF (also known as ARHGEF28), PLEKHG1, P-REX2, Solo (also known as ARHGEF40) and α-PIX (also known as ARHGEF6) – which specifically or broadly target RhoA, Rac1 and/or Cdc42, are involved in cyclic-stretch-induced perpendicular reorientation of endothelial cells. Overexpression of Solo induced RhoA activation and F-actin accumulation at cell-cell and cell-substrate adhesion sites. Knockdown of Solo suppressed cyclic-stretch- or tensile-force-induced RhoA activation. Moreover, knockdown of Solo significantly reduced cyclic-stretch-induced perpendicular reorientation of endothelial cells when cells were cultured at high density, but not when they were cultured at low density or pretreated with EGTA or VE-cadherin-targeting small interfering RNAs. These results suggest that Solo is involved in cell-cell-adhesion-mediated mechanical signal transduction during cyclic-stretch-induced endothelial cell reorientation.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Abiko H,Fujiwara S,Ohashi K,Hiatari R,Mashiko T,Sakamoto N,Sato M,Mizuno Kdoi
10.1242/jcs.157503subject
Has Abstractpub_date
2015-05-01 00:00:00pages
1683-95issue
9eissn
0021-9533issn
1477-9137pii
jcs.157503journal_volume
128pub_type
杂志文章abstract：:Homophilic binding of E-cadherins through their ectodomains is fundamental to epithelial cell-cell adhesion. Despite this, E-cadherin ectodomains have evolved differently in the vertebrate and insect lineages. Of the five rod-like, tandemly aligned extracellular cadherin domains of vertebrate E-cadherin, the tip extra...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.189258
更新日期：2016-09-01 00:00:00
abstract：:Super-resolution fluorescence imaging by single-molecule photoactivation or photoswitching and position determination (localization microscopy) has the potential to fundamentally revolutionize our understanding of how cellular function is encoded at the molecular level. Among all powerful, high-resolution imaging tech...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.123612
更新日期：2013-08-15 00:00:00
abstract：:Ubiquitinated membrane proteins such as epidermal growth factor receptor (EGFR) are delivered to early endosomes and then sorted to lysosomes via multivesicular bodies (MVBs) for degradation. The regulatory mechanism underlying formation of intralumenal vesicles en route to generation of MVBs is not fully understood. ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.229179
更新日期：2019-10-03 00:00:00
abstract：:Besides regulating glucose levels, insulin has been reported to participate actively in many other functions, including modulating inflammatory reactions. In this study we investigated how topical insulin application would affect the diabetic wound healing process. We found that the excessive expression of insulin-deg...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.235838
更新日期：2020-10-12 00:00:00
abstract：:SNARE [soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor] proteins control the membrane-fusion events of eukaryotic membrane-trafficking pathways. Specific vesicular and target SNAREs operate in specific trafficking routes, but the degree of specificity of SNARE functions is still elus...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.007948
更新日期：2007-09-15 00:00:00
abstract：:A cDNA encoding rat CaBP1 has been isolated and sequenced. The deduced polypeptide chain consists of 440 amino acids including two internal thioredoxin-like domains and a C-terminal KDEL retention/retrieval signal. Regarding the high degree of identity to the hamster protein P5, CaBP1 is considered to be the homologou...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：:Guinea pig sperm were suspended in calcium-containing medium supplemented with various concentrations of the tetrasaccharide, stachyose. At concentrations up to and including 0.6 M, stachyose was without effect on the A23187-induced acrosome reaction. At 1.0 M stachyose, greater than 97% of sperm retained their acroso...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Ezrin/radixin/moesin (ERM) proteins are thought to play an important role in organizing cortical actin-based cytoskeletons through cross-linkage of actin filaments with integral membrane proteins. Recent in vitro biochemical studies have revealed that ERM proteins phosphorylated on their COOH-terminal threonine residu...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:Mammalian homologs of the Drosophila TRP protein have been shown to form cation-permeable channels in the plasma membrane but very little is known about the mechanisms that control their cell surface localization. Recently it has been demonstrated that the last three C-terminal amino acids (TRL) of TRPC4 comprise a PD...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：:This study describes the functional characterization of two cell lines derived from the proximal convoluted (PKSV-PCT cells) and proximal straight (PKSV-PR) tubules microdissected out from kidneys of transgenic mice harboring the simian virus 40 (SV40) large T and small t antigens placed under the control of the rat L...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:Following fertilization, there are rapid changes in the appearance of the Fucus egg. Large electron-translucent vesicles (V1) accumulate fibrillar material, and following pronuclear fusion, they are largely electron-opaque. These vesicles (V1) are formed originally in unfertilized eggs by smooth endoplasmic reticulum ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1976-03-01 00:00:00
abstract：:Nuclear domains called ND10 or PML nuclear bodies consist of an aggregation of several proteins, most notably PML and Sp100. PML is essential in the nucleation and formation of ND10 as well as in the recruitment of other ND10-associated proteins such as Daxx, pRb, BLM and Sp100. In cells induced to overexpress Sp100, ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Mitosis involves considerable membrane remodelling and vesicular trafficking to generate two independent cells. Consequently, endocytosis and endocytic proteins are required for efficient mitotic progression and completion. Several endocytic proteins also participate in mitosis in an endocytosis-independent manner. He...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.105981
更新日期：2012-09-15 00:00:00
abstract：:Experimentally introduced tension on kinetochores and their centromeres has been shown to stabilize kinetochore attachment to microtubules, modify kinetochore directional instability, and regulate cell-cycle progression into anaphase. In mitosis, kinetochore tension and the stretch of centromere chromatin are produced...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Isoamyl alcohol (IAA) induces a phenotype that resembles pseudohyphae in the budding yeast Saccharomyces cerevisiae. We show here that IAA causes the rapid formation of linear chains of anucleate buds, each of which is accompanied by the formation of a septin ring at its neck. This process requires the activity of Swe...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00634
更新日期：2003-08-15 00:00:00
abstract：:We have identified and characterized two proteins in rat brain that bind to the neuron-specific tropomyosin isoform, TMBr3. The two proteins were identified by blot overlay assay, in which the proteins immobilized on the membrane were probed by epitope-tagged TMBr3, followed by detection with anti-epitope antibody. We...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:Patients with myotonic dystrophy type 1 exhibit a diversity of symptoms that affect many different organs. Among these are cognitive dysfunctions, the origin of which has remained elusive, partly because of the difficulty in accessing neural cells. Here, we have taken advantage of pluripotent stem cell lines derived f...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.116285
更新日期：2013-04-15 00:00:00
abstract：:BAG6 participates in protein quality control and, here, we address its role in endoplasmic-reticulum-associated degradation (ERAD) by using the polytopic membrane protein OpD, an opsin degron mutant. Both BAG6 knockdown and BAG6 overexpression delay OpD degradation; however, our data suggest that these two perturbatio...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.145565
更新日期：2014-07-01 00:00:00
abstract：:Nedd1 is a new member of the gamma-tubulin ring complex (gammaTuRC) and targets the gammaTuRC to the centrosomes for microtubule nucleation and spindle assembly in mitosis. Although its role is known, its functional regulation mechanism remains unclear. Here we report that the function of Nedd1 is regulated by Cdk1 an...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.042747
更新日期：2009-07-01 00:00:00
abstract：:The binding of GFP-tagged tetracycline repressor (TetR) molecules to chromosomally integrated tetracycline operator (tetO) sequence repeats has been used as a system to study chromosome behaviour microscopically in vivo. We found that these integrated transgenes influence the architecture of yeast interphase nuclei, a...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-03-15 00:00:00
abstract：:Microinjected GTP gamma S revealed three distinct steps in the exocytic transport of the temperature sensitive glycoprotein of vesicular stomatitis virus (ts-O45-G) from the ER to the cell surface in intact Vero cells. While COPII dependent export of ts-O45-G from the ER is blocked in cells injected with recombinant p...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:Major histocompatibility complex class I (MHC-I) molecules signal infection or transformation by engaging receptors on T lymphocytes. The spatial organization of MHC-I on the plasma membranes is important for this engagement. We and others have shown that MHC-I molecules, like other membrane proteins, are not uniforml...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.187112
更新日期：2016-09-01 00:00:00
abstract：:The repair of injured tissue must occur rapidly to prevent microbial invasion and maintain tissue integrity. Epithelial tissues in particular, which serve as a barrier against the external environment, must repair efficiently in order to restore their primary function. Here we analyze the effect of different parameter...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.109066
更新日期：2012-12-15 00:00:00
abstract：:Actin-based myosin motors have a pivotal role in intracellular trafficking in eukaryotic cells. The parasitic protozoan organism Leishmania expresses a novel class of myosin, myosin XXI (Myo21), which is preferentially localized at the proximal region of the flagellum. However, its function in this organism remains la...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.064725
更新日期：2010-06-15 00:00:00
abstract：:Actin polymerization is a crucial process during sperm capacitation. We have recently described the participation of FAK during actin polymerization in guinea pig spermatozoa. However, the mechanism by which FAK mediates these processes is unknown. Our previous data have shown that MAPK1 (hereafter referred to as ERK2...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.239186
更新日期：2020-04-28 00:00:00
abstract：:Recently, we demonstrated in cultured endothelial cells and in vivo that deficiency of an isoform of intersectin-1, ITSN-1s, impairs caveolae and clathrin-mediated endocytosis and functionally upregulates compensatory pathways and their morphological carriers (i.e. enlarged endocytic structures, membranous rings or tu...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.163030
更新日期：2015-04-15 00:00:00
abstract：:Phosphorylation regulates assembly and disassembly of proteins during endocytosis. In yeast, Prk1 and Ark1 phosphorylate factors after vesicle internalization leading to coat disassembly. Scd5, a protein phosphatase-1 (PP1)-targeting subunit, is proposed to regulate dephosphorylation of Prk1/Ark1 substrates to promote...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.098871
更新日期：2012-10-15 00:00:00
abstract：:Clathrin-mediated endocytosis requires cargo-specific adaptor proteins that recognize specific receptors and recruit them into coated pits. ARH [also called low-density lipoprotein receptor (LDLR) adaptor protein] serves as an adaptor for LDLR endocytosis in liver. However, ARH is dispensable for LDL uptake by some ot...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03217
更新日期：2006-10-15 00:00:00
abstract：:The intraerythrocytic developmental cycle of Plasmodium falciparum is completed with the release of up to 32 invasive daughter cells, the merozoites, into the blood stream. Before release, the final step of merozoite development is the assembly of the cortical pellicle, a multi-layered membrane structure. This unique ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.181230
更新日期：2016-02-15 00:00:00
abstract：:Cue1p is an N-terminally anchored endoplasmic reticulum (ER) protein essential for the activity of the two major yeast RING finger ubiquitin ligases (E3s) implicated in ER-associated degradation (ERAD). Cue1p contains a CUE domain, which for several proteins is known to bind ubiquitin. We now establish that the CUE do...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.044255
更新日期：2009-05-01 00:00:00
