Abstract:
AIM:To study the role of Twist gene in gastric cancer by gene silencing, including the potential of induction of apoptosis, cell cycle arrest, and proliferation inhibition in human malignant gastric SGC7901 cells. METHODS:The expression level of Twist in gastric cancer samples was measured by immunohistochemistry. The effects of Twist gene silencing were detected at both mRNA and protein levels by RT-PCR and Western blot. We also evaluated the cell proliferation and apoptosis by CCK-8 assay and flow cytometry. We determined the activity of caspase-3 and caspase-9 with a caspase activity assay kit. Cell cycle distribution was analyzed by flow cytometry. Cell migration and invasion ability was evaluated by wound scratch assay and Boyden chamber assay. RESULTS:Twist protein was highly expressed in gastric cancer samples. Twist gene silencing significantly induced apoptosis, cell cycle arrest at G0/G1 phase, proliferation inhibition, and reduced the ability of migration and invasion in human gastric cancer SGC7901 cells. Meanwhile, both caspase-3 and caspase-9 were activated. CONCLUSION:The Twist gene could serve as a potential molecular target for gene therapy of gastric cancer with targeted small interfering RNA.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Zhang H,Gong J,Kong D,Liu HYdoi
10.3748/wjg.v21.i10.2926subject
Has Abstractpub_date
2015-03-14 00:00:00pages
2926-36issue
10eissn
1007-9327issn
2219-2840journal_volume
21pub_type
杂志文章abstract:AIM:To explore the correlation between expression of somatostatin (SS), gastrin (GAS) and cell apoptosis regulation gene bcl-2/bax in large intestine carcinoma. METHODS:Sixty-two large intestine cancer tissue samples were randomly and retrospectively selected from patients with large intestine carcinoma. Immunohistoch...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i5.721
更新日期:2005-02-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v8.i5.857
更新日期:2002-10-01 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v18.i26.3409
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pub_type: 杂志文章,meta分析,评审
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更新日期:2016-03-28 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 社论,评审
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更新日期:2015-09-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,meta分析
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pub_type: 杂志文章,评审
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更新日期:2009-02-14 00:00:00
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pub_type: 杂志文章
doi:10.3748/wjg.v8.i3.505
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pub_type: 杂志文章,评审
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更新日期:2014-05-21 00:00:00
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pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i10.2542
更新日期:2014-03-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 临床试验,杂志文章
doi:10.3748/wjg.v19.i34.5671
更新日期:2013-09-14 00:00:00
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pub_type: 杂志文章,评审
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.3748/wjg.v9.i10.2211
更新日期:2003-10-01 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 社论,评审
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更新日期:2016-02-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v3.i4.225
更新日期:1997-12-15 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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更新日期:2015-11-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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更新日期:2013-04-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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更新日期:2003-04-01 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 临床试验,杂志文章
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更新日期:2003-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-01-14 00:00:00
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pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00