Abstract:
:Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related mortality worldwide. We have previously reported that LASP-1 is a downstream protein of the urokinase type plasminogen activator (uPA). Here we investigated the role of LASP-1 in HCC by a molecular and biological characterization of LASP-1 expression in human HCC specimens and in cultured HCC cells. We determined the LASP-1 mRNA expression levels in 55 HCC cases with different hepatic background disease. We identified 3 groups of patients with high, equal or low LASP-1 mRNA levels in HCC tissues compared to the peritumoral (PT) tissues. In particular we found that i) the HCCs displayed a higher LASP-1 mRNA level in HCC compared to PT tissues; ii) the expression levels of LASP-1 mRNA in female HCCs were significantly higher compared to male HCCs; iii) the cirrhotic HCCs displayed a higher LASP-1 mRNA. Further, the biological characterization of the ectopic LASP-1 overexpression in HCC cells, using MALDI-TOF mass spectrometer on the LASP-1 co-immunoprecipitated fractions, displayed vimentin as a novel putative partner of LASP-1. Our results suggest that LASP-1 mRNA overexpression may be mainly implicated in female HCCs and cirrhotic HCCs; and that LASP1 may play its role with vimentin in HCC cells.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Salvi A,Bongarzone I,Ferrari L,Abeni E,Arici B,De Bortoli M,Scuri S,Bonini D,Grossi I,Benetti A,Baiocchi G,Portolani N,De Petro Gdoi
10.3892/ijo.2015.2923subject
Has Abstractpub_date
2015-05-01 00:00:00pages
1901-12issue
5eissn
1019-6439issn
1791-2423journal_volume
46pub_type
杂志文章abstract::Diagnostic features of 102 consecutive cases of cancer reoccurrence in the conserved breast are reported. The sensitivity of palpation, mammography, cytology or ultrasonography was 75.5%, 58.7%, 77.4% and 77.8%, respectively. Mammography failures were not due to natural breast density and could probably be explained b...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.2.337
更新日期:1993-08-01 00:00:00
abstract::The aim of the present review was to summarize the current knowledge of the involvement of nestin in breast cancer (BC) pathogenesis. Nestin is a member of the class VI family of intermediate filament proteins, originally identified as a marker of neural stem cells and subsequently demonstrated to be expressed in BC a...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2018.4441
更新日期:2018-08-01 00:00:00
abstract::In solid tumours, necrosis is commonly found in the core region in response to metabolic stress that results from oxygen and glucose depletion (OGD) due to insufficient vascularization and has been implicated in tumour progression. We have previously shown that metabolic stress due to glucose depletion (GD) induces ne...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::Although radiation therapy is a powerful anticancer modality, radiation- induced stress response and gene expression with adaptive resistance may severely compromise the effectiveness of radiation. The function of rotundic acid (RA) on inducing apoptosis in the human breast cancer cell line MCF-7 has been investigated...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4544
更新日期:2018-11-01 00:00:00
abstract::The Pleomorphic adenoma gene 1 (PLAG1) is involved in various human neoplasias, including pleomorphic adenomas of the salivary glands. Moreover, the oncogenic role of PLAG1 was clearly demonstrated in two independent PLAG1 transgenic mouse founders, in which PLAG1 expression could be targeted to different tissues usin...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2008-05-01 00:00:00
abstract::CD34 is commonly used as an endothelial cell marker of tumor vessels. However, this marker detects not only newly formed, but also pre-existing large blood vessels. Nestin, a class VI intermediate filament protein, has recently received attention as a marker for detecting newly formed endothelial cells. In this study,...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-03-01 00:00:00
abstract::Survivin is a member of the inhibitor of apoptosis family, which is known to inhibit mitochondrial apoptosis. Survivin is highly expressed in cancers and plays an important role in cancer cell survival, and increased survivin expression is an unfavorable prognostic marker in cancer patients. YM155, a novel small-molec...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3077
更新日期:2015-09-01 00:00:00
abstract::Chemotherapy (CT) resistance in ovarian cancer is related to multiple factors, and assessment of these factors is necessary for the development of new drugs and therapeutic regimens. In an effort to identify such determinants, we evaluated the expression of approximately 21,000 genes using DNA microarray screening in ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Three genes on the short arm of chromosome 11 (WT1, IGF2 and HRAS) were hypomethylated in Wilms' tumour tissue compared to normal kidney tissue, and one (CALCA) was hypermethylated. IGF2 and HRAS showed evidence of allele-specific methylation, which may indicate genomic imprinting of the 11p15 region in some kidney ce...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::An altered expression of microRNAs (miRNAs) contributes both to the development of cancer and to the progression of the disease. Malignant tumours and tumour cell lines have widespread deregulated expressions of miRNAs compared to normal tissues. In this study, we investigated the expression profiles of 340 mammalian ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1420
更新日期:2012-07-01 00:00:00
abstract::The expression of microRNA-375 (miR-375) is significantly reduced in cancer tissues of maxillary sinus squamous cell carcinoma (MSSCC). The aim of this study was to investigate the functional significance of miR-375 and a possible regulatory role in the MSSCC networks. Restoration o...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1196
更新日期:2012-01-01 00:00:00
abstract::The tumour suppressor gene hypermethylated in cancer 1 (HIC1) is a transcriptional repressor, which functionally cooperates with p53. Loss of HIC1 function is associated with the development of various tumor entities. The aim of this study was to elucidate the relevance of CpG islan...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1367
更新日期:2012-05-01 00:00:00
abstract::The neuroepithelioma cell line CHP100 expresses low but detectable amounts of N-myc protein together with large amounts of c-myc protein. We have recently demonstrated that antisense inhibition of N-myc expression in CHP100 cells leads to decreased in vitro growth and alterations in cellular morphology without affecti...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.1.175
更新日期:1995-01-01 00:00:00
abstract::Conjugated linoleic acid (CLA) refers to a group of naturally occurring positional and geometrical conjugated dienoic isomers of linoleic acid (C18:2), of which the cis-9,trans-11 (c9,t11) and trans-10,cis-12 (t10,c12) isomers predominate. Accumulating evidence has demonstrated that CLA isomers are capable of inhibiti...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::Expression of the tumor suppressor gene p53 and the ras oncogene were examined in 46 tumor and nodal specimens of non-small cell lung cancer (NSCLC) using the antibodies p53 pAb 240 and ras Y13-259 respectively. p53 expression was elevated in 46% and ras p21 was over-expressed in 85% of the tumor specimens analyzed. F...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.1.4.403
更新日期:1992-09-01 00:00:00
abstract::To determine the relation between apoptosis and cytotoxicity the number of apoptotic (AP) cells was measured in MOLT-4 and HL-60 cultures treated with adriamycin, etoposide, cisplatin and 1-B-D-arabinofuranosylcytosine (ara-C) in the dose range inducing 20-95% growth inhibition. DNA degradation in individual AP cells ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.2.481
更新日期:1994-02-01 00:00:00
abstract::Tumor-bearing mice with two different locally growing malignant tumors (epithelial like, MCG 101; malignant melanoma, K1735-M2) were used to evaluate the putative role of prostaglandins for survival and local tumor growth in experimental cancer. Daily systemic injections of indomethacin (1 mu g/g bw) were used to bloc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.6.1405
更新日期:1995-12-01 00:00:00
abstract::Thermal ablation of solid tumors using conductive interstitial thermal therapy (CITT) produces coagulative necrosis in the center of ablation. Local changes in homeostasis for surviving tumor and systemic changes in circulation and distant organs must be understood and monitored in order to prevent tumor re-growth and...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.2185
更新日期:2014-02-01 00:00:00
abstract::Breast cancer ranks as the second most common cause of cancer death among women in the United States. Anticancer agents are an important component of breast cancer therapy. Drugs frequently used to treat breast cancer include methotrexate, 5-fluorouracil (5-FU), cyclophosphamide, anthracyclines, taxanes, trastuzumab, ...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
更新日期:2005-11-01 00:00:00
abstract::We previously reported in a retrospective study that CEA-based RT-PCR was useful for predicting the prognosis of patients with node-negative colorectal cancer. RT-PCR is well established for laboratory use, but many issues remain to be resolved prior to its clinical application. In addition to the false positive rate ...
journal_title:International journal of oncology
pub_type: 杂志文章,多中心研究
doi:
更新日期:2004-09-01 00:00:00
abstract::Epidemiologic studies on the protective role of omega-3 fatty acids (n:3) on breast cancer prevention remain inconclusive but studies in preclinical models provide more positive outcome. However, the mechanisms accounting for the protective effect of n:3 are not defined. In the present study, conducted in the N-methyl...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1133
更新日期:2011-11-01 00:00:00
abstract::Identification of sensitive and specific biomarkers for early detection and prognosis of prostate cancer is essential for timely and appropriate treatment of the disease in individual patients. We identified an RNA transcript with sequence homology to TRPM8 (melastatin-related transient receptor potential member 8) th...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00
abstract::Subsequently to the publication of the above paper, the authors have realized that Figs. 3 and 4 contained errors. Two sets of bands in Figs. 3D and 4B were erroneously placed during the process of figure preparation. In Fig. 3D, the β‑actin bands for the PANC‑1 and CFPAC‑1 cells were presented incorrectly, whereas in...
journal_title:International journal of oncology
pub_type: 杂志文章,已发布勘误
doi:10.3892/ijo.2019.4871
更新日期:2019-11-01 00:00:00
abstract::Hypericin is a potent agent in the photodynamic therapy of cancers and accumulates to a large extent in tumor tissue. To better understand the impact of hypericin aggregates present in the delivery vehicle on the biodistribution of the compound, we compared the in vivo tissue accumulation after administering hypericin...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-03-01 00:00:00
abstract::The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has emerged as a promising anticancer agent with high tumor-selective cytotoxicity. The congenital and acquired resistance of some cancer types including malignant melanoma and osteosarcoma impede the current TRAIL therapy of these cancers. Since fine...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4096
更新日期:2017-10-01 00:00:00
abstract::Adrenomedullin (AM) is a multifunctional 52-amino acid peptide. AM has several effects and acts as a growth factor in several types of cancer cells. Our previous study revealed that an AM antagonist (AMA) suppressed the growth of pancreatic tumors in mice, although its mechanism was not elucidated. In this study, we c...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000622
更新日期:2010-06-01 00:00:00
abstract::Although many chemotherapies have been developed for melanomas, successful therapy would be aided by the identification of intrinsic mechanisms that are crucial for melanoma survival. Here, we used resveratrol, a phytoalexin, as an anti-melanoma reagent. Applying resveratrol to various human and murine melanoma cell l...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2480
更新日期:2014-08-01 00:00:00
abstract::Recent studies have demonstrated that natural antisense transcripts, which are complementary sequences to messenger RNA, have important cellular functions such as the stabilization and silencing of mRNA. However, the possible contribution of antisense transcripts in hepatocellular c...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1382
更新日期:2012-06-01 00:00:00
abstract::The overall goal of the present study was to evaluate the chemotherapeutic and cancer‑protective properties of D‑erythro‑sphingosine (sphingosine) and C2‑ceramide using a human breast epithelial cell (HBEC) culture system, which represents multiple‑stages of breast carcinogenesis. The HBEC model includes Type I HBECs ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4641
更新日期:2019-02-01 00:00:00
abstract::Dideoxypetrosynol A, a polyacetylene from the marine sponge Petrosia sp., is known to exhibit significant selective cytotoxic activity against several human cancer cell lines. In the present study, we investigated further possible mechanisms by which dideoxypetrosynol A exerts its anti-proliferative action in cultured...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00