dPob/EMC is essential for biosynthesis of rhodopsin and other multi-pass membrane proteins in Drosophila photoreceptors.

abstract：:Deinococcus radiodurans (DR) survives in the presence of hundreds of double-stranded DNA (dsDNA) breaks by efficiently repairing such breaks. RecO, a protein that is essential for the extreme radioresistance of DR, is one of the major recombination mediator proteins in the RecA-loading process in the RecFOR pathway. H...

journal_title：eLife

authors： Hwang J,Kim JY,Kim C,Park S,Joo S,Kim SK,Lee NK

更新日期：2020-04-16 00:00:00

abstract：:Systemic blood pressure is determined, in part, by arterial smooth muscle cells (myocytes). Several Transient Receptor Potential (TRP) channels are proposed to be expressed in arterial myocytes, but it is unclear if these proteins control physiological blood pressure and contribute to hypertension in vivo. We generate...

journal_title：eLife

authors： Bulley S,Fernández-Peña C,Hasan R,Leo MD,Muralidharan P,Mackay CE,Evanson KW,Moreira-Junior L,Mata-Daboin A,Burris SK,Wang Q,Kuruvilla KP,Jaggar JH

更新日期：2018-12-04 00:00:00

abstract：:The straight-tusked elephants Palaeoloxodon spp. were widespread across Eurasia during the Pleistocene. Phylogenetic reconstructions using morphological traits have grouped them with Asian elephants (Elephas maximus), and many paleontologists place Palaeoloxodon within Elephas. Here, we report the recovery of full mit...

journal_title：eLife

authors： Meyer M,Palkopoulou E,Baleka S,Stiller M,Penkman KEH,Alt KW,Ishida Y,Mania D,Mallick S,Meijer T,Meller H,Nagel S,Nickel B,Ostritz S,Rohland N,Schauer K,Schüler T,Roca AL,Reich D,Shapiro B,Hofreiter M

更新日期：2017-06-06 00:00:00

abstract：:The restoration of homeostasis after tissue damage relies on proper spatial-temporal control of damage-induced apoptosis and compensatory proliferation. In Drosophila imaginal discs these processes are coordinated by the stress response pathway JNK. We demonstrate that JNK signaling induces a dose-dependent extension ...

journal_title：eLife

authors： Cosolo A,Jaiswal J,Csordás G,Grass I,Uhlirova M,Classen AK

更新日期：2019-02-08 00:00:00

abstract：:The link between the combined action of neuromodulators in the brain and global brain states remains a mystery. In this study, using biophysically realistic models of the thalamocortical network, we identified the critical intrinsic and synaptic mechanisms, associated with the putative action of acetylcholine (ACh), G...

journal_title：eLife

authors： Krishnan GP,Chauvette S,Shamie I,Soltani S,Timofeev I,Cash SS,Halgren E,Bazhenov M

更新日期：2016-11-16 00:00:00

abstract：:R-loops are features of chromatin consisting of a strand of DNA hybridized to RNA, as well as the expelled complementary DNA strand. R-loops are enriched at promoters where they have recently been shown to have important roles in modifying gene expression. However, the location of promoter-associated R-loops and the g...

journal_title：eLife

authors： Dumelie JG,Jaffrey SR

更新日期：2017-10-26 00:00:00

abstract：:Canonical transient receptor potential channels (TRPC) are involved in receptor-operated and/or store-operated Ca2+ signaling. Inhibition of TRPCs by small molecules was shown to be promising in treating renal diseases. In cells, the channels are regulated by calmodulin (CaM). Molecular details of both CaM and drug bi...

journal_title：eLife

authors： Vinayagam D,Quentin D,Yu-Strzelczyk J,Sitsel O,Merino F,Stabrin M,Hofnagel O,Yu M,Ledeboer MW,Nagel G,Malojcic G,Raunser S

更新日期：2020-11-25 00:00:00

abstract：:Responses of sensory neurons represent stimulus information, but are also influenced by internal state. For example, when monkeys direct their attention to a visual stimulus, the response gain of specific subsets of neurons in visual cortex changes. Here, we develop a functional model of population activity to investi...

journal_title：eLife

authors： Rabinowitz NC,Goris RL,Cohen M,Simoncelli EP

更新日期：2015-11-02 00:00:00

abstract：:Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher olfactory centre, the lateral horn (LH), is implicated in innate behaviour. However, our structural and functional understanding of the LH is scant, in large part due to a lack of sparse neurogenetic t...

journal_title：eLife

authors： Dolan MJ,Frechter S,Bates AS,Dan C,Huoviala P,Roberts RJ,Schlegel P,Dhawan S,Tabano R,Dionne H,Christoforou C,Close K,Sutcliffe B,Giuliani B,Li F,Costa M,Ihrke G,Meissner GW,Bock DD,Aso Y,Rubin GM,Jefferis GS

更新日期：2019-05-21 00:00:00

abstract：:Striatal dysfunction plays an important role in dystonia, but the striatal cell types that contribute to abnormal movements are poorly defined. We demonstrate that conditional deletion of the DYT1 dystonia protein torsinA in embryonic progenitors of forebrain cholinergic and GABAergic neurons causes dystonic-like twis...

journal_title：eLife

authors： Pappas SS,Darr K,Holley SM,Cepeda C,Mabrouk OS,Wong JM,LeWitt TM,Paudel R,Houlden H,Kennedy RT,Levine MS,Dauer WT

更新日期：2015-06-08 00:00:00

abstract：:The expression of E-Cadherin, a protein best known for its role in cell adhesion, regulates the onset of embryonic differentiation. ...

journal_title：eLife

authors： Sancho M,Rodríguez TA

更新日期：2013-12-17 00:00:00

abstract：:Plasmodium falciparum causes malaria in humans with over 450,000 deaths annually. The asexual blood stage involves invasion of erythrocytes by merozoites, in which they grow and divide to release daughter merozoites, which in turn invade new erythrocytes perpetuating the cycle responsible for malaria. A key step in me...

journal_title：eLife

authors： Chen L,Xu Y,Wong W,Thompson JK,Healer J,Goddard-Borger ED,Lawrence MC,Cowman AF

更新日期：2017-02-14 00:00:00

abstract：:The sense of ownership of one's body is important for survival, e.g., in defending the body against a threat. However, in addition to affecting behavior, it also affects perception of the world. In the case of visuospatial perception, it has been shown that the sense of ownership causes external space to be perceptual...

journal_title：eLife

authors： van der Hoort B,Reingardt M,Ehrsson HH

更新日期：2017-08-17 00:00:00

abstract：:Wnt plays important role during development and in various diseases. Because Wnts are lipidated and highly hydrophobic, they can only be purified in the presence of detergents, limiting their use in various in vitro and in vivo assays. We purified N-terminally tagged recombinant Wnt3a secreted from cells and accidenta...

journal_title：eLife

authors： Mihara E,Hirai H,Yamamoto H,Tamura-Kawakami K,Matano M,Kikuchi A,Sato T,Takagi J

更新日期：2016-02-23 00:00:00

abstract：:Tissue elongation and its control by spatiotemporal signals is a major developmental question. Currently, it is thought that Drosophila ovarian follicular epithelium elongation requires the planar polarization of the basal domain cytoskeleton and of the extra-cellular matrix, associated with a dynamic process of rotat...

journal_title：eLife

authors： Alégot H,Pouchin P,Bardot O,Mirouse V

更新日期：2018-02-08 00:00:00

abstract：:Progression through the mitotic cell cycle requires periodic regulation of gene function at the levels of transcription, translation, protein-protein interactions, post-translational modification and degradation. However, the role of alternative splicing (AS) in the temporal control of cell cycle is not well understoo...

journal_title：eLife

authors： Dominguez D,Tsai YH,Weatheritt R,Wang Y,Blencowe BJ,Wang Z

更新日期：2016-03-25 00:00:00

abstract：:Plasma membrane lipid composition must be maintained during growth and under environmental insult. In yeast, signaling mediated by TOR Complex 2 (TORC2)-dependent protein kinase Ypk1 controls lipid abundance and distribution in response to membrane stress. Ypk1, among other actions, alleviates negative regulation of L...

journal_title：eLife

authors： Muir A,Ramachandran S,Roelants FM,Timmons G,Thorner J

更新日期：2014-10-03 00:00:00

abstract：:Most neurogenesis in the mammalian brain is completed embryonically, but in certain areas the production of neurons continues throughout postnatal life. The functional properties of mature postnatally generated neurons often match those of their embryonically produced counterparts. However, we show here that in the ol...

journal_title：eLife

authors： Galliano E,Franzoni E,Breton M,Chand AN,Byrne DJ,Murthy VN,Grubb MS

更新日期：2018-04-20 00:00:00

abstract：:Analysis of the spatial distribution of endomembrane trafficking is fundamental to understand the mechanisms controlling cellular dynamics, cell homeostasy, and cell interaction with its external environment in normal and pathological situations. We present a semi-parametric framework to quantitatively analyze and vis...

journal_title：eLife

authors： Pécot T,Zengzhen L,Boulanger J,Salamero J,Kervrann C

更新日期：2018-08-09 00:00:00

abstract：:Journals are exploring new approaches to peer review in order to reduce bias, increase transparency and respond to author preferences. Funders are also getting involved. ...

journal_title：eLife

authors： Rodgers P

更新日期：2017-09-29 00:00:00

abstract：:Experience-dependent reorganisation of functional maps in the cerebral cortex is well described in the primary sensory cortices. However, there is relatively little evidence for such cortical reorganisation over the short-term. Using human somatosensory cortex as a model, we investigated the effects of a 24 hr gluing ...

journal_title：eLife

authors： Kolasinski J,Makin TR,Logan JP,Jbabdi S,Clare S,Stagg CJ,Johansen-Berg H

更新日期：2016-12-30 00:00:00

abstract：:Dephosphorylation of eukaryotic translation initiation factor 2a (eIF2a) restores protein synthesis at the waning of stress responses and requires a PP1 catalytic subunit and a regulatory subunit, PPP1R15A/GADD34 or PPP1R15B/CReP. Surprisingly, PPP1R15-PP1 binary complexes reconstituted in vitro lacked substrate selec...

journal_title：eLife

authors： Chen R,Rato C,Yan Y,Crespillo-Casado A,Clarke HJ,Harding HP,Marciniak SJ,Read RJ,Ron D

更新日期：2015-03-16 00:00:00

abstract：:Bacterial cells are encased in a peptidoglycan (PG) exoskeleton that protects them from osmotic lysis and specifies their distinct shapes. Cell wall hydrolases are required to enlarge this covalently closed macromolecule during growth, but how these autolytic enzymes are regulated remains poorly understood. Bacillus s...

journal_title：eLife

authors： Dobihal GS,Brunet YR,Flores-Kim J,Rudner DZ

更新日期：2019-12-06 00:00:00

abstract：:Advances in DNA sequencing have revolutionized our ability to read genomes. However, even in the most well-studied of organisms, the bacterium Escherichia coli, for ≈65% of promoters we remain ignorant of their regulation. Until we crack this regulatory Rosetta Stone, efforts to read and write genomes will remain haph...

journal_title：eLife

authors： Ireland WT,Beeler SM,Flores-Bautista E,McCarty NS,Röschinger T,Belliveau NM,Sweredoski MJ,Moradian A,Kinney JB,Phillips R

更新日期：2020-09-21 00:00:00

abstract：:Centrioles are microtubule-based organelles crucial for cell division, sensing and motility. In Caenorhabditis elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional equivalents across eukaryotic evolution. Whereas the molecular architecture of SAS-6 and its role...

journal_title：eLife

authors： Rogala KB,Dynes NJ,Hatzopoulos GN,Yan J,Pong SK,Robinson CV,Deane CM,Gönczy P,Vakonakis I

更新日期：2015-05-29 00:00:00

abstract：:The mechanisms underlying axon regeneration in mature neurons are relevant to the understanding of normal nervous system maintenance and for developing therapeutic strategies for injury. Here, we report novel pathways in axon regeneration, identified by extending our previous function-based screen using the C. elegans...

journal_title：eLife

authors： Kim KW,Tang NH,Piggott CA,Andrusiak MG,Park S,Zhu M,Kurup N,Cherra SJ 3rd,Wu Z,Chisholm AD,Jin Y

更新日期：2018-11-21 00:00:00

abstract：:Neurons form bona fide synapses with oligodendrocyte precursor cells (OPCs), but the circuit context of these neuron to OPC synapses remains incompletely understood. Using monosynaptically-restricted rabies virus tracing of OPC afferents, we identified extensive afferent synaptic inputs to OPCs residing in secondary m...

journal_title：eLife

authors： Mount CW,Yalçın B,Cunliffe-Koehler K,Sundaresh S,Monje M

更新日期：2019-10-18 00:00:00

abstract：:Mouse GnT1IP-L, and membrane-bound GnT1IP-S (MGAT4D) expressed in cultured cells inhibit MGAT1, the N-acetylglucosaminyltransferase that initiates the synthesis of hybrid and complex N-glycans. However, it is not known where in the secretory pathway GnT1IP-L inhibits MGAT1, nor whether GnT1IP-L inhibits other N-glycan...

journal_title：eLife

authors： Huang HH,Hassinen A,Sundaram S,Spiess AN,Kellokumpu S,Stanley P

更新日期：2015-09-15 00:00:00

abstract：:Allosteric HIV-1 integrase (IN) inhibitors (ALLINIs) are a promising new class of antiretroviral agents that disrupt proper viral maturation by inducing hyper-multimerization of IN. Here we show that lead pyridine-based ALLINI KF116 exhibits striking selectivity for IN tetramers versus lower order protein oligomers. I...

journal_title：eLife

authors： Koneru PC,Francis AC,Deng N,Rebensburg SV,Hoyte AC,Lindenberger J,Adu-Ampratwum D,Larue RC,Wempe MF,Engelman AN,Lyumkis D,Fuchs JR,Levy RM,Melikyan GB,Kvaratskhelia M

更新日期：2019-05-23 00:00:00

abstract：:Genomic rearrangements associated with speciation often result in variation in chromosome number among closely related species. Malassezia species show variable karyotypes ranging between six and nine chromosomes. Here, we experimentally identified all eight centromeres in M. sympodialis as 3-5-kb long kinetochore-bou...

journal_title：eLife

authors： Sankaranarayanan SR,Ianiri G,Coelho MA,Reza MH,Thimmappa BC,Ganguly P,Vadnala RN,Sun S,Siddharthan R,Tellgren-Roth C,Dawson TL Jnr,Heitman J,Sanyal K

更新日期：2020-01-20 00:00:00