Absence of unsaturated fatty acid synthesis in murine T lymphocytes.

abstract：:The techniques of in vivo magnetic resonance (MR) imaging and spectroscopy have been established over the past two decades. Recent applications of these methods to study human brain function have become a rapidly growing area of research. The development of methods using standard MR contrast agents within the cerebral...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shulman RG,Blamire AM,Rothman DL,McCarthy G

更新日期：1993-04-15 00:00:00

abstract：:Engraftment and maintenance of hematopoietic stem and progenitor cells (HSPC) depend on their ability to respond to extracellular signals from the bone marrow microenvironment, but the critical intracellular pathways integrating these signals remain poorly understood. Furthermore, recent studies provide contradictory ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sanchez-Aguilera A,Lee YJ,Lo Celso C,Ferraro F,Brumme K,Mondal S,Kim C,Dorrance A,Luo HR,Scadden DT,Williams DA

更新日期：2011-06-07 00:00:00

abstract：:Sera from 30 patients with astrocytoma were tested for antibody reacting with cell surface antigens of cultured autologous astrocytoma cells. Ten percent of the patients had antibody detectable by mixed hemadsorption assays, approximately 50% by immune adherence and protein A assays, and 100% by anti-C3-mixed hemadsor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pfreundschuh M,Shiku H,Takahashi T,Ueda R,Ransohoff J,Oettgen HF,Old LJ

更新日期：1978-10-01 00:00:00

abstract：:Ribosome crystal structures have revealed that two small subunit proteins, S9 and S13, have C-terminal tails, which, together with several features of 16S rRNA, contact the anticodon stem-loop of P-site tRNA. To test the functional importance of these protein tails, we created genomic deletions of the C-terminal regio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoang L,Fredrick K,Noller HF

更新日期：2004-08-24 00:00:00

abstract：:A general and efficient method has been developed to generate large numbers of single-base substitution mutations simply and rapidly. A unique f1 phage recombinant DNA cloning vector is described, which contains the phi X174 origin of viral strand DNA synthesis and allows one to direct mutagenesis to any specific segm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abarzúa P,Marians KJ

更新日期：1984-04-01 00:00:00

abstract：:Polyketides represent an extremely diverse class of secondary metabolites often explored for their bioactive traits. These molecules are also attractive building blocks for chemical catalysis and polymerization. However, the use of polyketides in larger scale chemistry applications is stymied by limited titers and yie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Markham KA,Palmer CM,Chwatko M,Wagner JM,Murray C,Vazquez S,Swaminathan A,Chakravarty I,Lynd NA,Alper HS

更新日期：2018-02-27 00:00:00

abstract：:Protein acetylation, especially histone acetylation, is the subject of both research and clinical investigation. At least four small-molecule histone deacetylase inhibitors are currently in clinical trials for the treatment of cancer. These and other inhibitors also affect microtubule acetylation. A multidimensional, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haggarty SJ,Koeller KM,Wong JC,Grozinger CM,Schreiber SL

更新日期：2003-04-15 00:00:00

abstract：:We examine the quantum confinement in the photoemission ionization energy in air and optical band gap of carbon nanoparticles (CNPs). Premixed, stretched-stabilized ethylene flames are used to generate the CNPs reproducibly over the range of 4-23 nm in volume median diameter. The results reveal that flame-formed CNPs ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu C,Singh AV,Saggese C,Tang Q,Chen D,Wan K,Vinciguerra M,Commodo M,De Falco G,Minutolo P,D'Anna A,Wang H

更新日期：2019-06-25 00:00:00

abstract：:Echoviruses are enteroviruses that belong to Picornaviridae. Many echoviruses use decay-accelerating factor (DAF) as their cellular receptor. DAF is a glycosylphosphatidyl inositol-anchored complement regulatory protein found on most cell surfaces. It functions to protect cells from complement attack. The cryo-electro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He Y,Lin F,Chipman PR,Bator CM,Baker TS,Shoham M,Kuhn RJ,Medof ME,Rossmann MG

更新日期：2002-08-06 00:00:00

abstract：:The fact that animal introns are not spliced out in plants suggests that recognition of pre-mRNA splice sites differs between the two kingdoms. In plants, little is known about proteins required for splicing, as no plant in vitro splicing system is available. Several essential splicing factors from animals, such as SF...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lopato S,Mayeda A,Krainer AR,Barta A

更新日期：1996-04-02 00:00:00

abstract：:The respiratory burst oxidase of neutrophils can be activated in a cell-free system in which plasma membranes, cytosol, Mg2+, and a membrane-perturbing detergent, such as arachidonate or sodium dodecyl sulfate, are all required. Using the technique of preparative isoelectric focusing, the cytosol factor required for o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Curnutte JT,Scott PJ,Mayo LA

更新日期：1989-02-01 00:00:00

abstract：:An in vitro system [Weil, P. A., Luse, D. S., Segall, J. & Roeder, R. G. (1979) Cell 18, 469-484 and Manley, J. L., Fire, A., Cano, A., Sharp, P. A. & Gefter, M. L., (1980) Proc. Natl. Acad. Sci USA 77, 3855-3859] was adapted for studying initiation of transcription of the ovalbumin gene. The DNA template was a cloned...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai SY,Tsai MJ,O'Malley BW

更新日期：1981-02-01 00:00:00

abstract：:Cyclin-dependent kinase 4/6 (CDK4/6) inhibitors are an established treatment in estrogen receptor-positive breast cancer and are currently in clinical development in melanoma, a tumor that exhibits high rates of CDK4 activation. We analyzed melanoma cells with acquired resistance to the CDK4/6 inhibitor palbociclib an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： AbuHammad S,Cullinane C,Martin C,Bacolas Z,Ward T,Chen H,Slater A,Ardley K,Kirby L,Chan KT,Brajanovski N,Smith LK,Rao AD,Lelliott EJ,Kleinschmidt M,Vergara IA,Papenfuss AT,Lau P,Ghosh P,Haupt S,Haupt Y,Sanij E,

更新日期：2019-09-03 00:00:00

abstract：:p53 is a master regulatory, sequence-specific transcription factor that directly controls expression of over 100 genes in response to various stress signals. Transactivation is generally considered to occur through p53 binding to a consensus response element (RE) composed of two 5'-RRRCWWGYYY-3' decamers. Recently, st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Menendez D,Inga A,Resnick MA

更新日期：2010-01-26 00:00:00

abstract：:IL-7 functions as a trophic factor during T lymphocyte development by a mechanism that is partly based on the induction of Bcl-2, which protects cells from apoptosis. Here we report a mechanism by which cytokine withdrawal activates the prodeath protein Bax. On loss of IL-7 in a dependent cell line, Bax protein transl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khaled AR,Kim K,Hofmeister R,Muegge K,Durum SK

更新日期：1999-12-07 00:00:00

abstract：:Ligands of the endothelial-enriched tunica interna endothelial cell kinase 2 (Tie2) are markedly imbalanced in severe infections associated with vascular leakage, yet regulation of the receptor itself has been understudied in this context. Here, we show that TIE2 gene expression may constitute a novel vascular barrier...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ghosh CC,David S,Zhang R,Berghelli A,Milam K,Higgins SJ,Hunter J,Mukherjee A,Wei Y,Tran M,Suber F,Kobzik L,Kain KC,Lu S,Santel A,Yano K,Guha P,Dumont DJ,Christiani DC,Parikh SM

更新日期：2016-03-01 00:00:00

abstract：:Thermotaxis by individual amoebae of Dictyostelium discoideum on a temperature gradient is described. These amoebae show positive thermotaxis at temperatures between 14 degrees C and 28 degrees C shortly (3 hr) after food depletion. Increasing time on the gradient reduces the positive thermotactic response at the lowe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong CB,Fontana DR,Poff KL

更新日期：1983-09-01 00:00:00

abstract：:In most human follicular B cell lymphomas the bcl-2 gene is up-regulated as a result of the t(14;18) chromosomal translocation generating a hybrid bcl-2-IgH mRNA. Recently, we have identified in t(14;18)-positive cells a bcl-2-IgH mRNA in the antisense orientation, putatively responsible for the overexpression of bcl-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morelli S,Delia D,Capaccioli S,Quattrone A,Schiavone N,Bevilacqua A,Tomasini S,Nicolin A

更新日期：1997-07-22 00:00:00

abstract：:Physiological sensing of O(2) tension (partial O(2) pressure, pO(2)) plays an important role in some mammalian cellular systems, but striated muscle generally is not considered to be among them. Here we describe a molecular mechanism in skeletal muscle that acutely couples changes in pO(2) to altered calcium release t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun QA,Hess DT,Nogueira L,Yong S,Bowles DE,Eu J,Laurita KR,Meissner G,Stamler JS

更新日期：2011-09-20 00:00:00

abstract：:The Alzheimer A beta amyloid peptide (A beta) is the principal proteinaceous component of amyloid associated with Alzheimer disease (AD). We have determined the relative abundance of A beta structural variants present in amyloid from brains of 10 individuals with sporadic AD, 2 individuals with familial AD carrying sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Näslund J,Schierhorn A,Hellman U,Lannfelt L,Roses AD,Tjernberg LO,Silberring J,Gandy SE,Winblad B,Greengard P

更新日期：1994-08-30 00:00:00

abstract：:Node characteristics and behaviors are often correlated with the structure of social networks over time. While evidence of this type of assortative mixing and temporal clustering of behaviors among linked nodes is used to support claims of peer influence and social contagion in networks, homophily may also explain suc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aral S,Muchnik L,Sundararajan A

更新日期：2009-12-22 00:00:00

abstract：:We show that the nonlinear mechanical response of networks formed from un-cross-linked fibrin or collagen type I continually changes in response to repeated large-strain loading. We demonstrate that this dynamic evolution of the mechanical response arises from a shift of a characteristic nonlinear stress-strain relati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Münster S,Jawerth LM,Leslie BA,Weitz JI,Fabry B,Weitz DA

更新日期：2013-07-23 00:00:00

abstract：:Container-shaped molecules provide structured environments that impart geometric bounds on the motions and conformations of smaller molecular occupants. Moreover, they provide "solvation" that is constrained in time and space. When inwardly directed functional groups are present, they can interact chemically with the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Purse BW,Rebek J Jr

更新日期：2005-08-02 00:00:00

abstract：:Addition of acetylcholine to growing cultures of mouse neuroblastoma cells induced a 37-fold increase in the specific activity of acetylcholinesterase (EC 3.1.1.7). Morphological changes, consisting of neurite-extensions, were also observed during the logarithmic phase of growth of cells stimulated with acetylcholine....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harkins J,Arsenault M,Schlesinger K,Kates J

更新日期：1972-11-01 00:00:00

abstract：:The effect of reflex splanchnic nerve stimulation on proenkephalin A biosynthesis was investigated in the rat adrenal medulla. Tissue levels of native [Met5]enkephalin-like immunoreactivity (IR) (measured by direct RIA of tissue extracts), cryptic [Met5]enkephalin-like IR (calculated as the increase in [Met5]enkephali...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kanamatsu T,Unsworth CD,Diliberto EJ Jr,Viveros OH,Hong JS

更新日期：1986-12-01 00:00:00

abstract：:HLA-DR2 is associated with susceptibility to multiple sclerosis (MS). A peptide from human myelin basic protein (MBP, residues 85-99) was previously found to bind to purified HLA-DR2 (DRA, DRB1*1501) and to be recognized by human MBP-specific T cell clones. Soluble HLA-DR2 was expressed in the baculovirus system by re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gauthier L,Smith KJ,Pyrdol J,Kalandadze A,Strominger JL,Wiley DC,Wucherpfennig KW

更新日期：1998-09-29 00:00:00

abstract：:We have determined the localization and orientation of two genetic probes within the human major histocompatibility complex by chromosomal in situ hybridization. Our data indicate that a cloned genomic probe cross-hybridizing to HLA-A, -B, and -C heavy chain loci is homologous to sequences located on chromosome 6 at b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morton CC,Kirsch IR,Nance WE,Evans GA,Korman AJ,Strominger JL

更新日期：1984-05-01 00:00:00

abstract：:Two high molecular weight heat shock proteins, HSP90 (Mr, 90,000) and HSP100 (Mr, 100,000), were separately purified from extracts of cultured cells of a mouse lymphoma cell line, L5178Y. Both of the HSPs exist in homodimeric form under physiological conditions. Their physicochemical properties are quite similar to ea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koyasu S,Nishida E,Kadowaki T,Matsuzaki F,Iida K,Harada F,Kasuga M,Sakai H,Yahara I

更新日期：1986-11-01 00:00:00

abstract：:Naive CD4 T cells differentiate into several effector lineages, which generate a stronger and more rapid response to previously encountered immunological challenges. Although effector function is a key feature of adaptive immunity, the molecular basis of this process is poorly understood. Here, we investigated the spa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zorca CE,Kim LK,Kim YJ,Krause MR,Zenklusen D,Spilianakis CG,Flavell RA

更新日期：2015-03-31 00:00:00

abstract：:Fuchs endothelial corneal dystrophy (FECD) is a leading cause of corneal endothelial (CE) degeneration resulting in impaired visual acuity. It is a genetically complex and age-related disorder, with higher incidence in females. In this study, we established a nongenetic FECD animal model based on the physiologic outco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu C,Miyajima T,Melangath G,Miyai T,Vasanth S,Deshpande N,Kumar V,Ong Tone S,Gupta R,Zhu S,Vojnovic D,Chen Y,Rogan EG,Mondal B,Zahid M,Jurkunas UV

更新日期：2020-01-07 00:00:00